HLA–DQA1 is not an apparent risk factor for microchimerism in patients with various autoimmune diseases and in healthy individuals
Objective Microchimeric cells have been identified in lesions and peripheral blood of patients with systemic sclerosis (SSc) and idiopathic inflammatory myopathies (IIM), and HLA–DQA1*0501 is a risk factor for these diseases in some populations. Furthermore, DQA1*0501 has been associated with T lymp...
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| Veröffentlicht in: | Arthritis and rheumatism 2003-09, Vol.48 (9), p.2567-2572 |
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| container_title | Arthritis and rheumatism |
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| creator | Artlett, Carol M. O'Hanlon, Terrence P. Lopez, Ana M. Song, Yeong Wook Miller, Frederick W. Rider, Lisa G. |
| description | Objective
Microchimeric cells have been identified in lesions and peripheral blood of patients with systemic sclerosis (SSc) and idiopathic inflammatory myopathies (IIM), and HLA–DQA1*0501 is a risk factor for these diseases in some populations. Furthermore, DQA1*0501 has been associated with T lymphocyte microchimerism in SSc. To better define the strength of this association, we assessed the relationship among DQA1 alleles and microchimerism.
Methods
DNA from whole peripheral blood or magnetically sorted T cells was tested for microchimeric cells by polymerase chain reaction of the Y chromosome or of HLA–Cw in 87 SSc patients, 28 juvenile IIM patients, and 88 healthy controls. Thirty‐seven mother–son pairs were also analyzed for microchimerism and DQA1*0501.
Results
We were unable to demonstrate that DQA1*0501 is associated with microchimerism in T lymphocytes or in whole peripheral blood DNA in patients with SSc or juvenile IIM or in healthy individuals. In the 37 mother–son pairs, we were unable to demonstrate an association of DQA1*0501 with microchimerism in peripheral blood DNA or T lymphocytes, and compatibility between the donor's and recipient's HLA alleles did not influence microchimerism in the recipient.
Conclusion
These data suggest that HLA–DQA1 alleles do not appear to play a role in the persistence of microchimerism in the peripheral blood or T lymphocytes of patients with selected autoimmune diseases or in healthy individuals. |
| doi_str_mv | 10.1002/art.11235 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73634891</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>73634891</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3515-54d1c3ededb7bf507e25bb3cfd5336c2ba79e0982f02d7df9e70a16f68168c8b3</originalsourceid><addsrcrecordid>eNp1kMtKLDEQhoMc0fGy8AUkGw-4aE06k74sB483GBBF1011UmGifTNJK7Nz4Rv4hj6J0RlwdRahkqovf1X9hBxwdsIZS0_BhRPOUyE3yITLtEwYF_wPmTDGpomQJd8mO94_xmdkxBbZjmXBpnk2Ie9X89nn28e_2xmn1tOuDxQ6CsMADrtAnfVP1IAKvaMmntYq16uFbTFWWmo7OkCwkfT01YYFfQFn-9FTGENv23bskGrrETzGXKe_PywQmrBYxqu2L1aP0Pg9smliwP113CUPF-f3Z1fJ_Oby-mw2T5SQXCZyqrkSqFHXeW0kyzGVdS2U0VKITKU15CWyskgNS3WuTYk5A56ZrOBZoYpa7JK_K93B9c8j-lC11itsGugwTl3lIhPTouQRPF6BcVvvHZpqcLYFt6w4q74tr6Ll1Y_lkT1ci451i_qXXHscgaM1AF5BYxx0yvpfLq5WyB-h0xX3ahtc_r9jNbu7X7X-Aonjmvk</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>73634891</pqid></control><display><type>article</type><title>HLA–DQA1 is not an apparent risk factor for microchimerism in patients with various autoimmune diseases and in healthy individuals</title><source>MEDLINE</source><source>Wiley Journals</source><creator>Artlett, Carol M. ; O'Hanlon, Terrence P. ; Lopez, Ana M. ; Song, Yeong Wook ; Miller, Frederick W. ; Rider, Lisa G.</creator><creatorcontrib>Artlett, Carol M. ; O'Hanlon, Terrence P. ; Lopez, Ana M. ; Song, Yeong Wook ; Miller, Frederick W. ; Rider, Lisa G.</creatorcontrib><description>Objective
Microchimeric cells have been identified in lesions and peripheral blood of patients with systemic sclerosis (SSc) and idiopathic inflammatory myopathies (IIM), and HLA–DQA1*0501 is a risk factor for these diseases in some populations. Furthermore, DQA1*0501 has been associated with T lymphocyte microchimerism in SSc. To better define the strength of this association, we assessed the relationship among DQA1 alleles and microchimerism.
Methods
DNA from whole peripheral blood or magnetically sorted T cells was tested for microchimeric cells by polymerase chain reaction of the Y chromosome or of HLA–Cw in 87 SSc patients, 28 juvenile IIM patients, and 88 healthy controls. Thirty‐seven mother–son pairs were also analyzed for microchimerism and DQA1*0501.
Results
We were unable to demonstrate that DQA1*0501 is associated with microchimerism in T lymphocytes or in whole peripheral blood DNA in patients with SSc or juvenile IIM or in healthy individuals. In the 37 mother–son pairs, we were unable to demonstrate an association of DQA1*0501 with microchimerism in peripheral blood DNA or T lymphocytes, and compatibility between the donor's and recipient's HLA alleles did not influence microchimerism in the recipient.
Conclusion
These data suggest that HLA–DQA1 alleles do not appear to play a role in the persistence of microchimerism in the peripheral blood or T lymphocytes of patients with selected autoimmune diseases or in healthy individuals.</description><identifier>ISSN: 0004-3591</identifier><identifier>EISSN: 1529-0131</identifier><identifier>DOI: 10.1002/art.11235</identifier><identifier>PMID: 13130476</identifier><identifier>CODEN: ARHEAW</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Adolescent ; Adult ; Aged ; Biological and medical sciences ; Child ; Child, Preschool ; Chimera ; Chromosomes, Human, Y ; Female ; Genetic Predisposition to Disease - epidemiology ; HLA-DQ alpha-Chains ; HLA-DQ Antigens - genetics ; Humans ; Male ; Maternal-Fetal Exchange - genetics ; Medical sciences ; Middle Aged ; Myositis - epidemiology ; Myositis - genetics ; Myositis - immunology ; Pregnancy ; Risk Factors ; Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis ; Scleroderma, Systemic - epidemiology ; Scleroderma, Systemic - genetics ; Scleroderma, Systemic - immunology ; T-Lymphocytes - physiology</subject><ispartof>Arthritis and rheumatism, 2003-09, Vol.48 (9), p.2567-2572</ispartof><rights>Copyright © 2003 by the American College of Rheumatology</rights><rights>2004 INIST-CNRS</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3515-54d1c3ededb7bf507e25bb3cfd5336c2ba79e0982f02d7df9e70a16f68168c8b3</citedby><cites>FETCH-LOGICAL-c3515-54d1c3ededb7bf507e25bb3cfd5336c2ba79e0982f02d7df9e70a16f68168c8b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fart.11235$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fart.11235$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15158535$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/13130476$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Artlett, Carol M.</creatorcontrib><creatorcontrib>O'Hanlon, Terrence P.</creatorcontrib><creatorcontrib>Lopez, Ana M.</creatorcontrib><creatorcontrib>Song, Yeong Wook</creatorcontrib><creatorcontrib>Miller, Frederick W.</creatorcontrib><creatorcontrib>Rider, Lisa G.</creatorcontrib><title>HLA–DQA1 is not an apparent risk factor for microchimerism in patients with various autoimmune diseases and in healthy individuals</title><title>Arthritis and rheumatism</title><addtitle>Arthritis Rheum</addtitle><description>Objective
Microchimeric cells have been identified in lesions and peripheral blood of patients with systemic sclerosis (SSc) and idiopathic inflammatory myopathies (IIM), and HLA–DQA1*0501 is a risk factor for these diseases in some populations. Furthermore, DQA1*0501 has been associated with T lymphocyte microchimerism in SSc. To better define the strength of this association, we assessed the relationship among DQA1 alleles and microchimerism.
Methods
DNA from whole peripheral blood or magnetically sorted T cells was tested for microchimeric cells by polymerase chain reaction of the Y chromosome or of HLA–Cw in 87 SSc patients, 28 juvenile IIM patients, and 88 healthy controls. Thirty‐seven mother–son pairs were also analyzed for microchimerism and DQA1*0501.
Results
We were unable to demonstrate that DQA1*0501 is associated with microchimerism in T lymphocytes or in whole peripheral blood DNA in patients with SSc or juvenile IIM or in healthy individuals. In the 37 mother–son pairs, we were unable to demonstrate an association of DQA1*0501 with microchimerism in peripheral blood DNA or T lymphocytes, and compatibility between the donor's and recipient's HLA alleles did not influence microchimerism in the recipient.
Conclusion
These data suggest that HLA–DQA1 alleles do not appear to play a role in the persistence of microchimerism in the peripheral blood or T lymphocytes of patients with selected autoimmune diseases or in healthy individuals.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Biological and medical sciences</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Chimera</subject><subject>Chromosomes, Human, Y</subject><subject>Female</subject><subject>Genetic Predisposition to Disease - epidemiology</subject><subject>HLA-DQ alpha-Chains</subject><subject>HLA-DQ Antigens - genetics</subject><subject>Humans</subject><subject>Male</subject><subject>Maternal-Fetal Exchange - genetics</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Myositis - epidemiology</subject><subject>Myositis - genetics</subject><subject>Myositis - immunology</subject><subject>Pregnancy</subject><subject>Risk Factors</subject><subject>Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis</subject><subject>Scleroderma, Systemic - epidemiology</subject><subject>Scleroderma, Systemic - genetics</subject><subject>Scleroderma, Systemic - immunology</subject><subject>T-Lymphocytes - physiology</subject><issn>0004-3591</issn><issn>1529-0131</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kMtKLDEQhoMc0fGy8AUkGw-4aE06k74sB483GBBF1011UmGifTNJK7Nz4Rv4hj6J0RlwdRahkqovf1X9hBxwdsIZS0_BhRPOUyE3yITLtEwYF_wPmTDGpomQJd8mO94_xmdkxBbZjmXBpnk2Ie9X89nn28e_2xmn1tOuDxQ6CsMADrtAnfVP1IAKvaMmntYq16uFbTFWWmo7OkCwkfT01YYFfQFn-9FTGENv23bskGrrETzGXKe_PywQmrBYxqu2L1aP0Pg9smliwP113CUPF-f3Z1fJ_Oby-mw2T5SQXCZyqrkSqFHXeW0kyzGVdS2U0VKITKU15CWyskgNS3WuTYk5A56ZrOBZoYpa7JK_K93B9c8j-lC11itsGugwTl3lIhPTouQRPF6BcVvvHZpqcLYFt6w4q74tr6Ll1Y_lkT1ci451i_qXXHscgaM1AF5BYxx0yvpfLq5WyB-h0xX3ahtc_r9jNbu7X7X-Aonjmvk</recordid><startdate>200309</startdate><enddate>200309</enddate><creator>Artlett, Carol M.</creator><creator>O'Hanlon, Terrence P.</creator><creator>Lopez, Ana M.</creator><creator>Song, Yeong Wook</creator><creator>Miller, Frederick W.</creator><creator>Rider, Lisa G.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200309</creationdate><title>HLA–DQA1 is not an apparent risk factor for microchimerism in patients with various autoimmune diseases and in healthy individuals</title><author>Artlett, Carol M. ; O'Hanlon, Terrence P. ; Lopez, Ana M. ; Song, Yeong Wook ; Miller, Frederick W. ; Rider, Lisa G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3515-54d1c3ededb7bf507e25bb3cfd5336c2ba79e0982f02d7df9e70a16f68168c8b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Biological and medical sciences</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Chimera</topic><topic>Chromosomes, Human, Y</topic><topic>Female</topic><topic>Genetic Predisposition to Disease - epidemiology</topic><topic>HLA-DQ alpha-Chains</topic><topic>HLA-DQ Antigens - genetics</topic><topic>Humans</topic><topic>Male</topic><topic>Maternal-Fetal Exchange - genetics</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Myositis - epidemiology</topic><topic>Myositis - genetics</topic><topic>Myositis - immunology</topic><topic>Pregnancy</topic><topic>Risk Factors</topic><topic>Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis</topic><topic>Scleroderma, Systemic - epidemiology</topic><topic>Scleroderma, Systemic - genetics</topic><topic>Scleroderma, Systemic - immunology</topic><topic>T-Lymphocytes - physiology</topic><toplevel>online_resources</toplevel><creatorcontrib>Artlett, Carol M.</creatorcontrib><creatorcontrib>O'Hanlon, Terrence P.</creatorcontrib><creatorcontrib>Lopez, Ana M.</creatorcontrib><creatorcontrib>Song, Yeong Wook</creatorcontrib><creatorcontrib>Miller, Frederick W.</creatorcontrib><creatorcontrib>Rider, Lisa G.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Arthritis and rheumatism</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Artlett, Carol M.</au><au>O'Hanlon, Terrence P.</au><au>Lopez, Ana M.</au><au>Song, Yeong Wook</au><au>Miller, Frederick W.</au><au>Rider, Lisa G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>HLA–DQA1 is not an apparent risk factor for microchimerism in patients with various autoimmune diseases and in healthy individuals</atitle><jtitle>Arthritis and rheumatism</jtitle><addtitle>Arthritis Rheum</addtitle><date>2003-09</date><risdate>2003</risdate><volume>48</volume><issue>9</issue><spage>2567</spage><epage>2572</epage><pages>2567-2572</pages><issn>0004-3591</issn><eissn>1529-0131</eissn><coden>ARHEAW</coden><abstract>Objective
Microchimeric cells have been identified in lesions and peripheral blood of patients with systemic sclerosis (SSc) and idiopathic inflammatory myopathies (IIM), and HLA–DQA1*0501 is a risk factor for these diseases in some populations. Furthermore, DQA1*0501 has been associated with T lymphocyte microchimerism in SSc. To better define the strength of this association, we assessed the relationship among DQA1 alleles and microchimerism.
Methods
DNA from whole peripheral blood or magnetically sorted T cells was tested for microchimeric cells by polymerase chain reaction of the Y chromosome or of HLA–Cw in 87 SSc patients, 28 juvenile IIM patients, and 88 healthy controls. Thirty‐seven mother–son pairs were also analyzed for microchimerism and DQA1*0501.
Results
We were unable to demonstrate that DQA1*0501 is associated with microchimerism in T lymphocytes or in whole peripheral blood DNA in patients with SSc or juvenile IIM or in healthy individuals. In the 37 mother–son pairs, we were unable to demonstrate an association of DQA1*0501 with microchimerism in peripheral blood DNA or T lymphocytes, and compatibility between the donor's and recipient's HLA alleles did not influence microchimerism in the recipient.
Conclusion
These data suggest that HLA–DQA1 alleles do not appear to play a role in the persistence of microchimerism in the peripheral blood or T lymphocytes of patients with selected autoimmune diseases or in healthy individuals.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>13130476</pmid><doi>10.1002/art.11235</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0004-3591 |
| ispartof | Arthritis and rheumatism, 2003-09, Vol.48 (9), p.2567-2572 |
| issn | 0004-3591 1529-0131 |
| language | eng |
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| source | MEDLINE; Wiley Journals |
| subjects | Adolescent Adult Aged Biological and medical sciences Child Child, Preschool Chimera Chromosomes, Human, Y Female Genetic Predisposition to Disease - epidemiology HLA-DQ alpha-Chains HLA-DQ Antigens - genetics Humans Male Maternal-Fetal Exchange - genetics Medical sciences Middle Aged Myositis - epidemiology Myositis - genetics Myositis - immunology Pregnancy Risk Factors Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis Scleroderma, Systemic - epidemiology Scleroderma, Systemic - genetics Scleroderma, Systemic - immunology T-Lymphocytes - physiology |
| title | HLA–DQA1 is not an apparent risk factor for microchimerism in patients with various autoimmune diseases and in healthy individuals |
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