Protective effect of melatonin on β-cell damage in streptozotocin-induced diabetes in rats
The aim of the present study was the evaluation of possible protective effects of melatonin against β-cell damage in streptozotocin-induced diabetes in rats. Malondialdehyde levels and glutathione peroxidase activity were measured in pancreatic homogenates. Pancreatic β-cells were examined by immuno...
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| Veröffentlicht in: | Acta histochemica 2003, Vol.105 (3), p.261-266 |
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| creator | Yavuz, Ozlem Cam, Meryem Bukan, Neslihan Guven, Aysel Silan, Fatma |
| description | The aim of the present study was the evaluation of possible protective effects of melatonin against β-cell damage in streptozotocin-induced diabetes in rats. Malondialdehyde levels and glutathione peroxidase activity were measured in pancreatic homogenates. Pancreatic β-cells were examined by immunohistochemical methods. Streptozotocin was injected intraperitoneally at a single dose of 60 mg/kg for induction of diabetes. Melatonin (200 μg/kg/day, ip) was injected for 3 days prior to administration of streptozotocin; these injections were continued until the end of the study (4 weeks). Streptozotocin induced a significant increase in malondialdehyde levels (p < 0.01) and a significant decrease in glutathione peroxidase activity (p < 0.05) in pancreatic tissue. Degeneration of islet cells and weak immunohistochemical staining of insulin was observed in diabetic rats. Treatment of diabetic rats with melatonin markedly reduced malondialdehyde production (p < 0.05) and increased glutathione peroxidase activity (p < 0.01) without affecting hyperglycemia. Increased staining of insulin and preservation of islet cells were apparent in the melatonin-treated diabetic rats. These data suggest that melatonin treatment has a therapeutic effect in diabetes by reduction of oxidative stress and preservation of pancreatic β-cell integrity. |
| doi_str_mv | 10.1078/0065-1281-00711 |
| format | Article |
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Malondialdehyde levels and glutathione peroxidase activity were measured in pancreatic homogenates. Pancreatic β-cells were examined by immunohistochemical methods. Streptozotocin was injected intraperitoneally at a single dose of 60 mg/kg for induction of diabetes. Melatonin (200 μg/kg/day, ip) was injected for 3 days prior to administration of streptozotocin; these injections were continued until the end of the study (4 weeks). Streptozotocin induced a significant increase in malondialdehyde levels (p < 0.01) and a significant decrease in glutathione peroxidase activity (p < 0.05) in pancreatic tissue. Degeneration of islet cells and weak immunohistochemical staining of insulin was observed in diabetic rats. Treatment of diabetic rats with melatonin markedly reduced malondialdehyde production (p < 0.05) and increased glutathione peroxidase activity (p < 0.01) without affecting hyperglycemia. Increased staining of insulin and preservation of islet cells were apparent in the melatonin-treated diabetic rats. These data suggest that melatonin treatment has a therapeutic effect in diabetes by reduction of oxidative stress and preservation of pancreatic β-cell integrity.</description><identifier>ISSN: 0065-1281</identifier><identifier>EISSN: 1618-0372</identifier><identifier>DOI: 10.1078/0065-1281-00711</identifier><identifier>PMID: 13677620</identifier><language>eng</language><publisher>Germany: Elsevier GmbH</publisher><subject>Animals ; anti-insulin antibody ; Blood Glucose - analysis ; Body Mass Index ; diabetes mellitus ; Diabetes Mellitus, Experimental - blood ; Diabetes Mellitus, Experimental - pathology ; Diabetes Mellitus, Experimental - prevention & control ; Islets of Langerhans - pathology ; Male ; melatonin ; Melatonin - therapeutic use ; oxidative stress ; Rats ; streptozotocin</subject><ispartof>Acta histochemica, 2003, Vol.105 (3), p.261-266</ispartof><rights>2003 Urban & Fischer Verlag</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c374t-221444bf86e47ce930c26195413ec9453516fae7cf52eee648d22baa3252dc833</citedby><cites>FETCH-LOGICAL-c374t-221444bf86e47ce930c26195413ec9453516fae7cf52eee648d22baa3252dc833</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1078/0065-1281-00711$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,782,786,3552,4026,27930,27931,27932,46002,64394</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/13677620$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yavuz, Ozlem</creatorcontrib><creatorcontrib>Cam, Meryem</creatorcontrib><creatorcontrib>Bukan, Neslihan</creatorcontrib><creatorcontrib>Guven, Aysel</creatorcontrib><creatorcontrib>Silan, Fatma</creatorcontrib><title>Protective effect of melatonin on β-cell damage in streptozotocin-induced diabetes in rats</title><title>Acta histochemica</title><addtitle>Acta Histochem</addtitle><description>The aim of the present study was the evaluation of possible protective effects of melatonin against β-cell damage in streptozotocin-induced diabetes in rats. Malondialdehyde levels and glutathione peroxidase activity were measured in pancreatic homogenates. Pancreatic β-cells were examined by immunohistochemical methods. Streptozotocin was injected intraperitoneally at a single dose of 60 mg/kg for induction of diabetes. Melatonin (200 μg/kg/day, ip) was injected for 3 days prior to administration of streptozotocin; these injections were continued until the end of the study (4 weeks). Streptozotocin induced a significant increase in malondialdehyde levels (p < 0.01) and a significant decrease in glutathione peroxidase activity (p < 0.05) in pancreatic tissue. Degeneration of islet cells and weak immunohistochemical staining of insulin was observed in diabetic rats. Treatment of diabetic rats with melatonin markedly reduced malondialdehyde production (p < 0.05) and increased glutathione peroxidase activity (p < 0.01) without affecting hyperglycemia. Increased staining of insulin and preservation of islet cells were apparent in the melatonin-treated diabetic rats. These data suggest that melatonin treatment has a therapeutic effect in diabetes by reduction of oxidative stress and preservation of pancreatic β-cell integrity.</description><subject>Animals</subject><subject>anti-insulin antibody</subject><subject>Blood Glucose - analysis</subject><subject>Body Mass Index</subject><subject>diabetes mellitus</subject><subject>Diabetes Mellitus, Experimental - blood</subject><subject>Diabetes Mellitus, Experimental - pathology</subject><subject>Diabetes Mellitus, Experimental - prevention & control</subject><subject>Islets of Langerhans - pathology</subject><subject>Male</subject><subject>melatonin</subject><subject>Melatonin - therapeutic use</subject><subject>oxidative stress</subject><subject>Rats</subject><subject>streptozotocin</subject><issn>0065-1281</issn><issn>1618-0372</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkD1LBDEQhoMoen7UdrKV3WomySa7pYhfcKCFVhYhl52VyO3mTHKC_ix_iL_JrHdoJVYZMs-8zDyEHAI9AarqU0plVQKroaRUAWyQCUioS8oV2ySTn-4O2Y3xmVLaUM62yQ5wqZRkdEIe74JPaJN7xQK7LleF74oe5yb5wQ2FH4rPj9LifF60pjdPWOTPmAIukn_3yVs3lG5olxbbonVmhgnjiAST4j7Z6sw84sH63SMPlxf359fl9Pbq5vxsWlquRCoZAyHErKslCmWx4dQyCU0lgKNtRMUrkJ1BZbuKIaIUdcvYzBjOKtbamvM9crzKXQT_ssSYdO_iuLIZ0C-jVlxyzvLp_4EcBFAGLIOnK9AGH2PATi-C601400D1KF6PavWoVn-LzxNH6-jlrMf2l1-bzkCzAjCbeHUYdLQOh-zNhWxdt979Gf4FFP-QXA</recordid><startdate>2003</startdate><enddate>2003</enddate><creator>Yavuz, Ozlem</creator><creator>Cam, Meryem</creator><creator>Bukan, Neslihan</creator><creator>Guven, Aysel</creator><creator>Silan, Fatma</creator><general>Elsevier GmbH</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>2003</creationdate><title>Protective effect of melatonin on β-cell damage in streptozotocin-induced diabetes in rats</title><author>Yavuz, Ozlem ; Cam, Meryem ; Bukan, Neslihan ; Guven, Aysel ; Silan, Fatma</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c374t-221444bf86e47ce930c26195413ec9453516fae7cf52eee648d22baa3252dc833</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>anti-insulin antibody</topic><topic>Blood Glucose - analysis</topic><topic>Body Mass Index</topic><topic>diabetes mellitus</topic><topic>Diabetes Mellitus, Experimental - blood</topic><topic>Diabetes Mellitus, Experimental - pathology</topic><topic>Diabetes Mellitus, Experimental - prevention & control</topic><topic>Islets of Langerhans - pathology</topic><topic>Male</topic><topic>melatonin</topic><topic>Melatonin - therapeutic use</topic><topic>oxidative stress</topic><topic>Rats</topic><topic>streptozotocin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yavuz, Ozlem</creatorcontrib><creatorcontrib>Cam, Meryem</creatorcontrib><creatorcontrib>Bukan, Neslihan</creatorcontrib><creatorcontrib>Guven, Aysel</creatorcontrib><creatorcontrib>Silan, Fatma</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Acta histochemica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yavuz, Ozlem</au><au>Cam, Meryem</au><au>Bukan, Neslihan</au><au>Guven, Aysel</au><au>Silan, Fatma</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protective effect of melatonin on β-cell damage in streptozotocin-induced diabetes in rats</atitle><jtitle>Acta histochemica</jtitle><addtitle>Acta Histochem</addtitle><date>2003</date><risdate>2003</risdate><volume>105</volume><issue>3</issue><spage>261</spage><epage>266</epage><pages>261-266</pages><issn>0065-1281</issn><eissn>1618-0372</eissn><abstract>The aim of the present study was the evaluation of possible protective effects of melatonin against β-cell damage in streptozotocin-induced diabetes in rats. Malondialdehyde levels and glutathione peroxidase activity were measured in pancreatic homogenates. Pancreatic β-cells were examined by immunohistochemical methods. Streptozotocin was injected intraperitoneally at a single dose of 60 mg/kg for induction of diabetes. Melatonin (200 μg/kg/day, ip) was injected for 3 days prior to administration of streptozotocin; these injections were continued until the end of the study (4 weeks). Streptozotocin induced a significant increase in malondialdehyde levels (p < 0.01) and a significant decrease in glutathione peroxidase activity (p < 0.05) in pancreatic tissue. Degeneration of islet cells and weak immunohistochemical staining of insulin was observed in diabetic rats. Treatment of diabetic rats with melatonin markedly reduced malondialdehyde production (p < 0.05) and increased glutathione peroxidase activity (p < 0.01) without affecting hyperglycemia. Increased staining of insulin and preservation of islet cells were apparent in the melatonin-treated diabetic rats. These data suggest that melatonin treatment has a therapeutic effect in diabetes by reduction of oxidative stress and preservation of pancreatic β-cell integrity.</abstract><cop>Germany</cop><pub>Elsevier GmbH</pub><pmid>13677620</pmid><doi>10.1078/0065-1281-00711</doi><tpages>6</tpages></addata></record> |
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| ispartof | Acta histochemica, 2003, Vol.105 (3), p.261-266 |
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| source | MEDLINE; Access via ScienceDirect (Elsevier); ProQuest Central UK/Ireland |
| subjects | Animals anti-insulin antibody Blood Glucose - analysis Body Mass Index diabetes mellitus Diabetes Mellitus, Experimental - blood Diabetes Mellitus, Experimental - pathology Diabetes Mellitus, Experimental - prevention & control Islets of Langerhans - pathology Male melatonin Melatonin - therapeutic use oxidative stress Rats streptozotocin |
| title | Protective effect of melatonin on β-cell damage in streptozotocin-induced diabetes in rats |
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