Genetic diversity of human isolates of Salmonella enterica serovar Enteritidis in Malaysia

Aims: The study was undertaken to determine clonal relationship and genetic diversity of the human strains of Salmonella enterica serovar Enteritidis isolated from 1995 to 2002 from different parts of Malaysia. Methods and Results: Antimicrobial susceptibility test, plasmid profiling and pulsed‐fiel...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of applied microbiology 2003-01, Vol.95 (4), p.773-780
Hauptverfasser: Bakeri, S.A., Yasin, R.M., Koh, Y.T., Puthucheary, S.D., Thong, K.L.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 780
container_issue 4
container_start_page 773
container_title Journal of applied microbiology
container_volume 95
creator Bakeri, S.A.
Yasin, R.M.
Koh, Y.T.
Puthucheary, S.D.
Thong, K.L.
description Aims: The study was undertaken to determine clonal relationship and genetic diversity of the human strains of Salmonella enterica serovar Enteritidis isolated from 1995 to 2002 from different parts of Malaysia. Methods and Results: Antimicrobial susceptibility test, plasmid profiling and pulsed‐field gel electrophoresis were applied to analyse 65 human isolates of S. Enteritidis obtained over an eight year period from different parts of Malaysia. Four nonhuman isolates were included for comparison. A total of 14 distinct XbaI–pulsed‐field profiles (PFPs) were observed, although a single PFP X1 was predominant and this particular clone was found to be endemic in Malaysia. The incidence of drug resistant S. Enteritidis remained relatively low with only 37% of the strains analysed being resistant to one or more antimicrobial agents. All except one resistant strain carried at least one plasmid ranging in size from 3·7 to 62 MDa giving nine plasmid profiles. The three isolates from raw milk and one from well‐water had similar PFPs to that of the human isolates. Conclusions: Salmonella Enteritidis strains were more diverse than was previously thought. Fourteen subtypes were noted although one predominant clone persisted in Malaysia. The combination of pulsed‐field gel electrophoresis, plasmid profiling and antibiograms provided additional discrimination to the highly clonal strains of S. Enteritidis. Significance and Impact of the Study: This is the first report to assess the genotypes of the predominant clinical S. Enteritidis in different parts of the country. As S. Enteritidis is highly endemic in Malaysia, the data generated would be useful for tracing the source during outbreaks of gastroenteritis in the study area.
doi_str_mv 10.1046/j.1365-2672.2003.02033.x
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73628705</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>18876467</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5193-a337cd52496687b1aa9ab42a454f0311b26572b49bde2b231de4b60f5363dd143</originalsourceid><addsrcrecordid>eNqNkUtv1EAMgEcIREvLX0AjJLglzNjzSA4cqqoUUCsO0AuXkZNMxKzyKDNJ6f57kt0VlXopJ1v2Z8vWxxiXIpdCmQ-bXKLRGRgLOQiBuQCBmN8_Y8f_Gs93ucq0sHDEXqW0EUKi0OYlO5JQmhJKecx-XvrBT6HmTbjzMYVpy8eW_5p7GnhIY0eTT2vlO3X9OPiuI-6HycdQE08-jncU-cWuMIUmJB4Gfk0dbVOgU_aipS7514d4wm4-Xfw4_5xdfbv8cn52ldValpgRoq0bDao0prCVJCqpUkBKq1aglBUYbaFSZdV4qABl41VlRKvRYNNIhSfs_X7vbRx_zz5Nrg-pXk8d_DgnZ9FAYYV-EpRFYY0ydgHfPgI34xyH5QkHCKXGEswCFXuojmNK0bfuNoae4tZJ4VZLbuNWGW6V4VZLbmfJ3S-jbw7756r3zcPgQcsCvDsAlGrq2khDHdIDpxeRWMDCfdxzf0Lnt_99gPt6dr1m-BdVeaxL</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>232953926</pqid></control><display><type>article</type><title>Genetic diversity of human isolates of Salmonella enterica serovar Enteritidis in Malaysia</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><source>Oxford University Press Journals All Titles (1996-Current)</source><creator>Bakeri, S.A. ; Yasin, R.M. ; Koh, Y.T. ; Puthucheary, S.D. ; Thong, K.L.</creator><creatorcontrib>Bakeri, S.A. ; Yasin, R.M. ; Koh, Y.T. ; Puthucheary, S.D. ; Thong, K.L.</creatorcontrib><description>Aims: The study was undertaken to determine clonal relationship and genetic diversity of the human strains of Salmonella enterica serovar Enteritidis isolated from 1995 to 2002 from different parts of Malaysia. Methods and Results: Antimicrobial susceptibility test, plasmid profiling and pulsed‐field gel electrophoresis were applied to analyse 65 human isolates of S. Enteritidis obtained over an eight year period from different parts of Malaysia. Four nonhuman isolates were included for comparison. A total of 14 distinct XbaI–pulsed‐field profiles (PFPs) were observed, although a single PFP X1 was predominant and this particular clone was found to be endemic in Malaysia. The incidence of drug resistant S. Enteritidis remained relatively low with only 37% of the strains analysed being resistant to one or more antimicrobial agents. All except one resistant strain carried at least one plasmid ranging in size from 3·7 to 62 MDa giving nine plasmid profiles. The three isolates from raw milk and one from well‐water had similar PFPs to that of the human isolates. Conclusions: Salmonella Enteritidis strains were more diverse than was previously thought. Fourteen subtypes were noted although one predominant clone persisted in Malaysia. The combination of pulsed‐field gel electrophoresis, plasmid profiling and antibiograms provided additional discrimination to the highly clonal strains of S. Enteritidis. Significance and Impact of the Study: This is the first report to assess the genotypes of the predominant clinical S. Enteritidis in different parts of the country. As S. Enteritidis is highly endemic in Malaysia, the data generated would be useful for tracing the source during outbreaks of gastroenteritis in the study area.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1046/j.1365-2672.2003.02033.x</identifier><identifier>PMID: 12969291</identifier><identifier>CODEN: JAMIFK</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Anti-Bacterial Agents - pharmacology ; antibiogram ; Bacteriology ; Biological and medical sciences ; DNA, Bacterial - analysis ; Drug Resistance, Bacterial ; Electrophoresis, Gel, Pulsed-Field - methods ; Fundamental and applied biological sciences. Psychology ; Genetics ; Humans ; Malaysia ; Microbial Sensitivity Tests - methods ; Microbiology ; PFGE ; plasmids ; Plasmids - genetics ; Retrospective Studies ; Salmonella ; Salmonella Enteritidis ; Salmonella enteritidis - drug effects ; Salmonella enteritidis - genetics</subject><ispartof>Journal of applied microbiology, 2003-01, Vol.95 (4), p.773-780</ispartof><rights>2004 INIST-CNRS</rights><rights>Copyright Blackwell Science Ltd. 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5193-a337cd52496687b1aa9ab42a454f0311b26572b49bde2b231de4b60f5363dd143</citedby><cites>FETCH-LOGICAL-c5193-a337cd52496687b1aa9ab42a454f0311b26572b49bde2b231de4b60f5363dd143</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-2672.2003.02033.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-2672.2003.02033.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=15130382$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12969291$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bakeri, S.A.</creatorcontrib><creatorcontrib>Yasin, R.M.</creatorcontrib><creatorcontrib>Koh, Y.T.</creatorcontrib><creatorcontrib>Puthucheary, S.D.</creatorcontrib><creatorcontrib>Thong, K.L.</creatorcontrib><title>Genetic diversity of human isolates of Salmonella enterica serovar Enteritidis in Malaysia</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aims: The study was undertaken to determine clonal relationship and genetic diversity of the human strains of Salmonella enterica serovar Enteritidis isolated from 1995 to 2002 from different parts of Malaysia. Methods and Results: Antimicrobial susceptibility test, plasmid profiling and pulsed‐field gel electrophoresis were applied to analyse 65 human isolates of S. Enteritidis obtained over an eight year period from different parts of Malaysia. Four nonhuman isolates were included for comparison. A total of 14 distinct XbaI–pulsed‐field profiles (PFPs) were observed, although a single PFP X1 was predominant and this particular clone was found to be endemic in Malaysia. The incidence of drug resistant S. Enteritidis remained relatively low with only 37% of the strains analysed being resistant to one or more antimicrobial agents. All except one resistant strain carried at least one plasmid ranging in size from 3·7 to 62 MDa giving nine plasmid profiles. The three isolates from raw milk and one from well‐water had similar PFPs to that of the human isolates. Conclusions: Salmonella Enteritidis strains were more diverse than was previously thought. Fourteen subtypes were noted although one predominant clone persisted in Malaysia. The combination of pulsed‐field gel electrophoresis, plasmid profiling and antibiograms provided additional discrimination to the highly clonal strains of S. Enteritidis. Significance and Impact of the Study: This is the first report to assess the genotypes of the predominant clinical S. Enteritidis in different parts of the country. As S. Enteritidis is highly endemic in Malaysia, the data generated would be useful for tracing the source during outbreaks of gastroenteritis in the study area.</description><subject>Anti-Bacterial Agents - pharmacology</subject><subject>antibiogram</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>DNA, Bacterial - analysis</subject><subject>Drug Resistance, Bacterial</subject><subject>Electrophoresis, Gel, Pulsed-Field - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetics</subject><subject>Humans</subject><subject>Malaysia</subject><subject>Microbial Sensitivity Tests - methods</subject><subject>Microbiology</subject><subject>PFGE</subject><subject>plasmids</subject><subject>Plasmids - genetics</subject><subject>Retrospective Studies</subject><subject>Salmonella</subject><subject>Salmonella Enteritidis</subject><subject>Salmonella enteritidis - drug effects</subject><subject>Salmonella enteritidis - genetics</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUtv1EAMgEcIREvLX0AjJLglzNjzSA4cqqoUUCsO0AuXkZNMxKzyKDNJ6f57kt0VlXopJ1v2Z8vWxxiXIpdCmQ-bXKLRGRgLOQiBuQCBmN8_Y8f_Gs93ucq0sHDEXqW0EUKi0OYlO5JQmhJKecx-XvrBT6HmTbjzMYVpy8eW_5p7GnhIY0eTT2vlO3X9OPiuI-6HycdQE08-jncU-cWuMIUmJB4Gfk0dbVOgU_aipS7514d4wm4-Xfw4_5xdfbv8cn52ldValpgRoq0bDao0prCVJCqpUkBKq1aglBUYbaFSZdV4qABl41VlRKvRYNNIhSfs_X7vbRx_zz5Nrg-pXk8d_DgnZ9FAYYV-EpRFYY0ydgHfPgI34xyH5QkHCKXGEswCFXuojmNK0bfuNoae4tZJ4VZLbuNWGW6V4VZLbmfJ3S-jbw7756r3zcPgQcsCvDsAlGrq2khDHdIDpxeRWMDCfdxzf0Lnt_99gPt6dr1m-BdVeaxL</recordid><startdate>20030101</startdate><enddate>20030101</enddate><creator>Bakeri, S.A.</creator><creator>Yasin, R.M.</creator><creator>Koh, Y.T.</creator><creator>Puthucheary, S.D.</creator><creator>Thong, K.L.</creator><general>Blackwell Science Ltd</general><general>Blackwell Science</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20030101</creationdate><title>Genetic diversity of human isolates of Salmonella enterica serovar Enteritidis in Malaysia</title><author>Bakeri, S.A. ; Yasin, R.M. ; Koh, Y.T. ; Puthucheary, S.D. ; Thong, K.L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5193-a337cd52496687b1aa9ab42a454f0311b26572b49bde2b231de4b60f5363dd143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Anti-Bacterial Agents - pharmacology</topic><topic>antibiogram</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>DNA, Bacterial - analysis</topic><topic>Drug Resistance, Bacterial</topic><topic>Electrophoresis, Gel, Pulsed-Field - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetics</topic><topic>Humans</topic><topic>Malaysia</topic><topic>Microbial Sensitivity Tests - methods</topic><topic>Microbiology</topic><topic>PFGE</topic><topic>plasmids</topic><topic>Plasmids - genetics</topic><topic>Retrospective Studies</topic><topic>Salmonella</topic><topic>Salmonella Enteritidis</topic><topic>Salmonella enteritidis - drug effects</topic><topic>Salmonella enteritidis - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bakeri, S.A.</creatorcontrib><creatorcontrib>Yasin, R.M.</creatorcontrib><creatorcontrib>Koh, Y.T.</creatorcontrib><creatorcontrib>Puthucheary, S.D.</creatorcontrib><creatorcontrib>Thong, K.L.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bakeri, S.A.</au><au>Yasin, R.M.</au><au>Koh, Y.T.</au><au>Puthucheary, S.D.</au><au>Thong, K.L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic diversity of human isolates of Salmonella enterica serovar Enteritidis in Malaysia</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2003-01-01</date><risdate>2003</risdate><volume>95</volume><issue>4</issue><spage>773</spage><epage>780</epage><pages>773-780</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><coden>JAMIFK</coden><abstract>Aims: The study was undertaken to determine clonal relationship and genetic diversity of the human strains of Salmonella enterica serovar Enteritidis isolated from 1995 to 2002 from different parts of Malaysia. Methods and Results: Antimicrobial susceptibility test, plasmid profiling and pulsed‐field gel electrophoresis were applied to analyse 65 human isolates of S. Enteritidis obtained over an eight year period from different parts of Malaysia. Four nonhuman isolates were included for comparison. A total of 14 distinct XbaI–pulsed‐field profiles (PFPs) were observed, although a single PFP X1 was predominant and this particular clone was found to be endemic in Malaysia. The incidence of drug resistant S. Enteritidis remained relatively low with only 37% of the strains analysed being resistant to one or more antimicrobial agents. All except one resistant strain carried at least one plasmid ranging in size from 3·7 to 62 MDa giving nine plasmid profiles. The three isolates from raw milk and one from well‐water had similar PFPs to that of the human isolates. Conclusions: Salmonella Enteritidis strains were more diverse than was previously thought. Fourteen subtypes were noted although one predominant clone persisted in Malaysia. The combination of pulsed‐field gel electrophoresis, plasmid profiling and antibiograms provided additional discrimination to the highly clonal strains of S. Enteritidis. Significance and Impact of the Study: This is the first report to assess the genotypes of the predominant clinical S. Enteritidis in different parts of the country. As S. Enteritidis is highly endemic in Malaysia, the data generated would be useful for tracing the source during outbreaks of gastroenteritis in the study area.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>12969291</pmid><doi>10.1046/j.1365-2672.2003.02033.x</doi><tpages>8</tpages></addata></record>
fulltext fulltext
identifier ISSN: 1364-5072
ispartof Journal of applied microbiology, 2003-01, Vol.95 (4), p.773-780
issn 1364-5072
1365-2672
language eng
recordid cdi_proquest_miscellaneous_73628705
source MEDLINE; Access via Wiley Online Library; Oxford University Press Journals All Titles (1996-Current)
subjects Anti-Bacterial Agents - pharmacology
antibiogram
Bacteriology
Biological and medical sciences
DNA, Bacterial - analysis
Drug Resistance, Bacterial
Electrophoresis, Gel, Pulsed-Field - methods
Fundamental and applied biological sciences. Psychology
Genetics
Humans
Malaysia
Microbial Sensitivity Tests - methods
Microbiology
PFGE
plasmids
Plasmids - genetics
Retrospective Studies
Salmonella
Salmonella Enteritidis
Salmonella enteritidis - drug effects
Salmonella enteritidis - genetics
title Genetic diversity of human isolates of Salmonella enterica serovar Enteritidis in Malaysia
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T10%3A16%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Genetic%20diversity%20of%20human%20isolates%20of%20Salmonella%20enterica%20serovar%20Enteritidis%20in%20Malaysia&rft.jtitle=Journal%20of%20applied%20microbiology&rft.au=Bakeri,%20S.A.&rft.date=2003-01-01&rft.volume=95&rft.issue=4&rft.spage=773&rft.epage=780&rft.pages=773-780&rft.issn=1364-5072&rft.eissn=1365-2672&rft.coden=JAMIFK&rft_id=info:doi/10.1046/j.1365-2672.2003.02033.x&rft_dat=%3Cproquest_cross%3E18876467%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=232953926&rft_id=info:pmid/12969291&rfr_iscdi=true