The Chamber/Dish: An Improved Vessel for Cell and Explant Culture
A vessel consisting of a Teflon well former, metal base plate, rubber gasket, and variable growth surface has been constructed. It is nontoxic, autoclavable, and reusable. It provides for ready access into the growth chamber, plus an optically clear lower surface for improved microscopic resolution....
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| Veröffentlicht in: | In Vitro 1981-02, Vol.17 (2), p.91-97 |
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| Format: | Artikel |
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| container_end_page | 97 |
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| container_start_page | 91 |
| container_title | In Vitro |
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| creator | Gabridge, Michael G. |
| description | A vessel consisting of a Teflon well former, metal base plate, rubber gasket, and variable growth surface has been constructed. It is nontoxic, autoclavable, and reusable. It provides for ready access into the growth chamber, plus an optically clear lower surface for improved microscopic resolution. The vessel has been used successfully with several different eucaryotic and procaryotic cell systems. Morphology of cells and yields (as measured by total protein and ATP) are comparable to those noted with other vessels. This Chamber/Dish can accommodate almost any type of growth substrate in the form of films or sheets. It appears to have widespread application for the growth of cells and explants in vitro. |
| doi_str_mv | 10.1007/BF02618064 |
| format | Article |
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It appears to have widespread application for the growth of cells and explants in vitro.</description><identifier>ISSN: 0073-5655</identifier><identifier>EISSN: 1475-2689</identifier><identifier>DOI: 10.1007/BF02618064</identifier><identifier>PMID: 6792053</identifier><language>eng</language><publisher>United States: Tissue Culture Association, Inc</publisher><subject>Animals ; Bacteriological Techniques ; Bottles ; Cell culture techniques ; Cell Division ; Cell growth ; Cell Line ; Cells, Cultured - cytology ; Cricetinae ; Culture Techniques - instrumentation ; Cytological Techniques - instrumentation ; Fibroblasts ; Gaskets ; Humans ; Lungs ; Magnification ; Microscopy ; Mycoplasma pneumoniae - growth & development ; Rubber ; Vero cells</subject><ispartof>In Vitro, 1981-02, Vol.17 (2), p.91-97</ispartof><rights>Copyright 1981 Tissue Culture Association</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c303t-e98a9ffed6f41880bf66719c6f4836be648a02292982bcee9e94299cfa3eccd63</citedby><cites>FETCH-LOGICAL-c303t-e98a9ffed6f41880bf66719c6f4836be648a02292982bcee9e94299cfa3eccd63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4292477$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4292477$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6792053$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gabridge, Michael G.</creatorcontrib><title>The Chamber/Dish: An Improved Vessel for Cell and Explant Culture</title><title>In Vitro</title><addtitle>In Vitro</addtitle><description>A vessel consisting of a Teflon well former, metal base plate, rubber gasket, and variable growth surface has been constructed. 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It appears to have widespread application for the growth of cells and explants in vitro.</description><subject>Animals</subject><subject>Bacteriological Techniques</subject><subject>Bottles</subject><subject>Cell culture techniques</subject><subject>Cell Division</subject><subject>Cell growth</subject><subject>Cell Line</subject><subject>Cells, Cultured - cytology</subject><subject>Cricetinae</subject><subject>Culture Techniques - instrumentation</subject><subject>Cytological Techniques - instrumentation</subject><subject>Fibroblasts</subject><subject>Gaskets</subject><subject>Humans</subject><subject>Lungs</subject><subject>Magnification</subject><subject>Microscopy</subject><subject>Mycoplasma pneumoniae - growth & development</subject><subject>Rubber</subject><subject>Vero cells</subject><issn>0073-5655</issn><issn>1475-2689</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkEtLw0AUhQdRaq1uXCvMyoUQO49kHu5qbLVQcFPdhsnkDm1JmjqTiP57R1rq6nL5Pg6Hg9A1JQ-UEDl-mhEmqCIiPUFDmsosYULpUzSMkCeZyLJzdBHChhBOBKMDNBBSM5LxIZosV4DzlWlK8OPndVg94skWz5udb7-gwh8QAtTYtR7nUNfYbCs8_d7VZtvhvK-73sMlOnOmDnB1uCP0Ppsu89dk8fYyzyeLxHLCuwS0Mto5qIRLqVKkdEJIqm18FRcliFQZwphmWrHSAmjQKdPaOsPB2krwEbrb58Zqnz2ErmjWwcZSZgttHwrJBRMyI1G834vWtyF4cMXOrxvjfwpKir-9iv-9onx7SO3LBqqjehgo8ps934Su9Uccq7FUSv4L0OtswQ</recordid><startdate>198102</startdate><enddate>198102</enddate><creator>Gabridge, Michael G.</creator><general>Tissue Culture Association, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198102</creationdate><title>The Chamber/Dish: An Improved Vessel for Cell and Explant Culture</title><author>Gabridge, Michael G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c303t-e98a9ffed6f41880bf66719c6f4836be648a02292982bcee9e94299cfa3eccd63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1981</creationdate><topic>Animals</topic><topic>Bacteriological Techniques</topic><topic>Bottles</topic><topic>Cell culture techniques</topic><topic>Cell Division</topic><topic>Cell growth</topic><topic>Cell Line</topic><topic>Cells, Cultured - cytology</topic><topic>Cricetinae</topic><topic>Culture Techniques - instrumentation</topic><topic>Cytological Techniques - instrumentation</topic><topic>Fibroblasts</topic><topic>Gaskets</topic><topic>Humans</topic><topic>Lungs</topic><topic>Magnification</topic><topic>Microscopy</topic><topic>Mycoplasma pneumoniae - growth & development</topic><topic>Rubber</topic><topic>Vero cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gabridge, Michael G.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>In Vitro</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gabridge, Michael G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Chamber/Dish: An Improved Vessel for Cell and Explant Culture</atitle><jtitle>In Vitro</jtitle><addtitle>In Vitro</addtitle><date>1981-02</date><risdate>1981</risdate><volume>17</volume><issue>2</issue><spage>91</spage><epage>97</epage><pages>91-97</pages><issn>0073-5655</issn><eissn>1475-2689</eissn><abstract>A vessel consisting of a Teflon well former, metal base plate, rubber gasket, and variable growth surface has been constructed. 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| ispartof | In Vitro, 1981-02, Vol.17 (2), p.91-97 |
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| language | eng |
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| source | Jstor Complete Legacy; MEDLINE; SpringerLink Journals |
| subjects | Animals Bacteriological Techniques Bottles Cell culture techniques Cell Division Cell growth Cell Line Cells, Cultured - cytology Cricetinae Culture Techniques - instrumentation Cytological Techniques - instrumentation Fibroblasts Gaskets Humans Lungs Magnification Microscopy Mycoplasma pneumoniae - growth & development Rubber Vero cells |
| title | The Chamber/Dish: An Improved Vessel for Cell and Explant Culture |
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