An immunofluorescence method for monitoring DNA synthesis by flow cytometry

An immunofluorescence method for monitoring DNA synthesis in single cells has been developed for flow cytometry. With antiserum which is specific for 5‐bromodeoxyuridine (BrdUrd) and a second fluorescent label, BrdUrd‐incorporation pulses of 30 min are detectable. The fluorescence intensity of the i...

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Veröffentlicht in:	Cytometry (New York, N.Y.) N.Y.), 1981-05, Vol.1 (6), p.385-389
Hauptverfasser:	Gratzner, H. G., Leif, R. C.
Format:	Artikel
Sprache:	eng
Schlagworte:	anti‐bromouridine antibodies Bromodeoxyuridine - immunology Bromodeoxyuridine - metabolism cell kinetics Cell Line DNA - biosynthesis DNA synthesis flow cytometry Flow Cytometry - methods Fluorescent Antibody Technique Humans Immune Sera immunofluorescence
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