Somatic hybrids between Arabidopsis thaliana and cytoplasmic male-sterile radish (Rapkanus sativus)
Somatic hybrids were produced by protoplast fusion between Arabidopsis thaliana ecotype Columbia and a male-sterile radish line MS-Gensuke ( Raphanus sativus) with the Ogura cytoplasm. Forty-one shoots were differentiated from the regenerated calli and established as shoot cultures in vitro. About 2...
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| Veröffentlicht in: | Plant cell reports 2003-08, Vol.22 (1), p.52-58 |
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| description | Somatic hybrids were produced by protoplast fusion between Arabidopsis thaliana ecotype Columbia and a male-sterile radish line MS-Gensuke ( Raphanus sativus) with the Ogura cytoplasm. Forty-one shoots were differentiated from the regenerated calli and established as shoot cultures in vitro. About 20 of these shoots were judged to be hybrids based on growth characteristics and morphology. Molecular analyses of 11 shoots were performed, confirming the hybrid features. Of these 11 shoots, eight were established as rooted plants in the greenhouse. Polymerase chain reaction and randomly amplified polymorphic DNA analyses of the nuclear genomes of all analyzed shoots and plants confirmed that they contained hybrid DNA patterns. Their chromosome numbers also supported the hybrid nature of the plants. Investigations of the organelles in the hybrids revealed that the chloroplast (cp) genome was exclusively represented by radish cpDNA, while the mitochondrial DNA configuration showed a combination of both parental genomes as well as fragments unique to the hybrids. Hybrid plants that flowered were male-sterile independent of the presence of the Ogura CMS-gene orf138. |
| doi_str_mv | 10.1007/s00299-003-0655-0 |
| format | Article |
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Forty-one shoots were differentiated from the regenerated calli and established as shoot cultures in vitro. About 20 of these shoots were judged to be hybrids based on growth characteristics and morphology. Molecular analyses of 11 shoots were performed, confirming the hybrid features. Of these 11 shoots, eight were established as rooted plants in the greenhouse. Polymerase chain reaction and randomly amplified polymorphic DNA analyses of the nuclear genomes of all analyzed shoots and plants confirmed that they contained hybrid DNA patterns. Their chromosome numbers also supported the hybrid nature of the plants. Investigations of the organelles in the hybrids revealed that the chloroplast (cp) genome was exclusively represented by radish cpDNA, while the mitochondrial DNA configuration showed a combination of both parental genomes as well as fragments unique to the hybrids. Hybrid plants that flowered were male-sterile independent of the presence of the Ogura CMS-gene orf138.</description><identifier>ISSN: 0721-7714</identifier><identifier>EISSN: 1432-203X</identifier><identifier>DOI: 10.1007/s00299-003-0655-0</identifier><identifier>PMID: 12827437</identifier><identifier>CODEN: PCRPD8</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Agronomy. Soil science and plant productions ; Arabidopsis - genetics ; Arabidopsis - growth & development ; Biological and medical sciences ; Biotechnology ; Culture Techniques ; Deoxyribonucleic acid ; DNA ; Eukaryotic cell cultures ; Fertility - genetics ; Fundamental and applied biological sciences. Psychology ; Genetics ; Genetics and breeding of economic plants ; Haploidy, in vitro culture applications, somatic hybrids ; Hybridization, Genetic - genetics ; Hybridization, Genetic - physiology ; Hybrids ; Methods. Procedures. Technologies ; Mitochondrial DNA ; Plant breeding: fundamental aspects and methodology ; Plant cells and fungal cells ; Plant Shoots - genetics ; Plant Shoots - growth & development ; Plants, Genetically Modified ; Ploidies ; Protoplast preparation, cell fusion and regeneration ; Raphanus - genetics ; Raphanus - growth & development</subject><ispartof>Plant cell reports, 2003-08, Vol.22 (1), p.52-58</ispartof><rights>2004 INIST-CNRS</rights><rights>Springer-Verlag 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c451t-bd3c5abef189a7340e3ea80268997c94268697a422b7eb5513c756f534738b4e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15071445$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12827437$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>YAMAGISHI, H</creatorcontrib><creatorcontrib>GLIMELIUS, K</creatorcontrib><title>Somatic hybrids between Arabidopsis thaliana and cytoplasmic male-sterile radish (Rapkanus sativus)</title><title>Plant cell reports</title><addtitle>Plant Cell Rep</addtitle><description>Somatic hybrids were produced by protoplast fusion between Arabidopsis thaliana ecotype Columbia and a male-sterile radish line MS-Gensuke ( Raphanus sativus) with the Ogura cytoplasm. Forty-one shoots were differentiated from the regenerated calli and established as shoot cultures in vitro. About 20 of these shoots were judged to be hybrids based on growth characteristics and morphology. Molecular analyses of 11 shoots were performed, confirming the hybrid features. Of these 11 shoots, eight were established as rooted plants in the greenhouse. Polymerase chain reaction and randomly amplified polymorphic DNA analyses of the nuclear genomes of all analyzed shoots and plants confirmed that they contained hybrid DNA patterns. Their chromosome numbers also supported the hybrid nature of the plants. Investigations of the organelles in the hybrids revealed that the chloroplast (cp) genome was exclusively represented by radish cpDNA, while the mitochondrial DNA configuration showed a combination of both parental genomes as well as fragments unique to the hybrids. Hybrid plants that flowered were male-sterile independent of the presence of the Ogura CMS-gene orf138.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - growth & development</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Culture Techniques</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Eukaryotic cell cultures</subject><subject>Fertility - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetics</subject><subject>Genetics and breeding of economic plants</subject><subject>Haploidy, in vitro culture applications, somatic hybrids</subject><subject>Hybridization, Genetic - genetics</subject><subject>Hybridization, Genetic - physiology</subject><subject>Hybrids</subject><subject>Methods. Procedures. Technologies</subject><subject>Mitochondrial DNA</subject><subject>Plant breeding: fundamental aspects and methodology</subject><subject>Plant cells and fungal cells</subject><subject>Plant Shoots - genetics</subject><subject>Plant Shoots - growth & development</subject><subject>Plants, Genetically Modified</subject><subject>Ploidies</subject><subject>Protoplast preparation, cell fusion and regeneration</subject><subject>Raphanus - genetics</subject><subject>Raphanus - growth & development</subject><issn>0721-7714</issn><issn>1432-203X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkcuKFEEQRRNRnHb0A9xIISi6SI18Vy2HwRcMCD7AXRKVlUXnWC8zqpT-e7PphgE3riIgzr0QHMaeCngjANxbApBNwwEUB2sMh3tsJ7SSXIL6cZ_twEnBnRP6gj0iugUoR2cfsgsha-nKvmPh6zzimkK1P7Q5dVS1cf0T41RdZWxTNy-UqFr3OCScsMKpq8JhnZcBaSyhEYfIaY05DbHK2CXaV6--4PITp40qKsW_N3r9mD3ocaD45Dwv2ff3775df-Q3nz98ur664UEbsfK2U8FgG3tRN-iUhqgi1iBt3TQuNLostnGopWxdbI0RKjhje6O0U3Wro7pkL0-9S55_bZFWPyYKcRhwivNG3ikrARrzX1DUNVjduAI-_we8nbc8lSd8LYwSVttjmzhBIc9EOfZ-yWnEfPAC_NGTP3nyxZM_evJQMs_OxVs7xu4ucRZTgBdnACng0GecQqI7zkDRqo36C6pxmi8</recordid><startdate>20030801</startdate><enddate>20030801</enddate><creator>YAMAGISHI, H</creator><creator>GLIMELIUS, K</creator><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7QO</scope><scope>7X8</scope></search><sort><creationdate>20030801</creationdate><title>Somatic hybrids between Arabidopsis thaliana and cytoplasmic male-sterile radish (Rapkanus sativus)</title><author>YAMAGISHI, H ; GLIMELIUS, K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c451t-bd3c5abef189a7340e3ea80268997c94268697a422b7eb5513c756f534738b4e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - growth & development</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Culture Techniques</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Eukaryotic cell cultures</topic><topic>Fertility - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetics</topic><topic>Genetics and breeding of economic plants</topic><topic>Haploidy, in vitro culture applications, somatic hybrids</topic><topic>Hybridization, Genetic - genetics</topic><topic>Hybridization, Genetic - physiology</topic><topic>Hybrids</topic><topic>Methods. Procedures. Technologies</topic><topic>Mitochondrial DNA</topic><topic>Plant breeding: fundamental aspects and methodology</topic><topic>Plant cells and fungal cells</topic><topic>Plant Shoots - genetics</topic><topic>Plant Shoots - growth & development</topic><topic>Plants, Genetically Modified</topic><topic>Ploidies</topic><topic>Protoplast preparation, cell fusion and regeneration</topic><topic>Raphanus - genetics</topic><topic>Raphanus - growth & development</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>YAMAGISHI, H</creatorcontrib><creatorcontrib>GLIMELIUS, K</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant cell reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>YAMAGISHI, H</au><au>GLIMELIUS, K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Somatic hybrids between Arabidopsis thaliana and cytoplasmic male-sterile radish (Rapkanus sativus)</atitle><jtitle>Plant cell reports</jtitle><addtitle>Plant Cell Rep</addtitle><date>2003-08-01</date><risdate>2003</risdate><volume>22</volume><issue>1</issue><spage>52</spage><epage>58</epage><pages>52-58</pages><issn>0721-7714</issn><eissn>1432-203X</eissn><coden>PCRPD8</coden><abstract>Somatic hybrids were produced by protoplast fusion between Arabidopsis thaliana ecotype Columbia and a male-sterile radish line MS-Gensuke ( Raphanus sativus) with the Ogura cytoplasm. Forty-one shoots were differentiated from the regenerated calli and established as shoot cultures in vitro. About 20 of these shoots were judged to be hybrids based on growth characteristics and morphology. Molecular analyses of 11 shoots were performed, confirming the hybrid features. Of these 11 shoots, eight were established as rooted plants in the greenhouse. Polymerase chain reaction and randomly amplified polymorphic DNA analyses of the nuclear genomes of all analyzed shoots and plants confirmed that they contained hybrid DNA patterns. Their chromosome numbers also supported the hybrid nature of the plants. Investigations of the organelles in the hybrids revealed that the chloroplast (cp) genome was exclusively represented by radish cpDNA, while the mitochondrial DNA configuration showed a combination of both parental genomes as well as fragments unique to the hybrids. Hybrid plants that flowered were male-sterile independent of the presence of the Ogura CMS-gene orf138.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>12827437</pmid><doi>10.1007/s00299-003-0655-0</doi><tpages>7</tpages></addata></record> |
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| subjects | Agronomy. Soil science and plant productions Arabidopsis - genetics Arabidopsis - growth & development Biological and medical sciences Biotechnology Culture Techniques Deoxyribonucleic acid DNA Eukaryotic cell cultures Fertility - genetics Fundamental and applied biological sciences. Psychology Genetics Genetics and breeding of economic plants Haploidy, in vitro culture applications, somatic hybrids Hybridization, Genetic - genetics Hybridization, Genetic - physiology Hybrids Methods. Procedures. Technologies Mitochondrial DNA Plant breeding: fundamental aspects and methodology Plant cells and fungal cells Plant Shoots - genetics Plant Shoots - growth & development Plants, Genetically Modified Ploidies Protoplast preparation, cell fusion and regeneration Raphanus - genetics Raphanus - growth & development |
| title | Somatic hybrids between Arabidopsis thaliana and cytoplasmic male-sterile radish (Rapkanus sativus) |
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