Chromatin superstructure: The next level of structure above the nucleosome has an alternating character. A two-nucleosome based series is generated by probes armed with DNAase-I acting on isolated nuclei
Intact nuclei were probed with DNAase-I-based probes and the resultant single strand pieces indicate that many or all of the nucleosomes exist in structures that cause every second nucleosome in a series to be resistant to attack. The effect is observed with chicken erythrocyte nuclei, rat liver nuc...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1981-04, Vol.99 (3), p.893-899 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Burgoyne, L.A. Skinner, J.D. |
| description | Intact nuclei were probed with DNAase-I-based probes and the resultant single strand pieces indicate that many or all of the nucleosomes exist in structures that cause every second nucleosome in a series to be resistant to attack. The effect is observed with chicken erythrocyte nuclei, rat liver nuclei and is weakly elicited by free DNAase-I. A method is described for the suppression of the intrinsic (CaMg) endonuclease of rat liver nuclei. |
| doi_str_mv | 10.1016/0006-291X(81)91247-X |
| format | Article |
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A method is described for the suppression of the intrinsic (CaMg) endonuclease of rat liver nuclei.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/0006-291X(81)91247-X</identifier><identifier>PMID: 6264918</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Cell Nucleus - analysis ; Cell Nucleus - drug effects ; Chickens ; Chromatin - analysis ; Chromatin - ultrastructure ; Deoxyribonuclease I ; Deoxyribonucleases - pharmacology ; Endonucleases - pharmacology ; Erythrocytes - analysis ; Liver - analysis ; Macromolecular Substances ; Nucleosomes - analysis ; Rats</subject><ispartof>Biochemical and biophysical research communications, 1981-04, Vol.99 (3), p.893-899</ispartof><rights>1981</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c272t-502760a8861b47054f5bdc4d06ae1471d4cb53ff1e4b854788272fd52ffb8ba03</citedby><cites>FETCH-LOGICAL-c272t-502760a8861b47054f5bdc4d06ae1471d4cb53ff1e4b854788272fd52ffb8ba03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0006291X8191247X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6264918$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Burgoyne, L.A.</creatorcontrib><creatorcontrib>Skinner, J.D.</creatorcontrib><title>Chromatin superstructure: The next level of structure above the nucleosome has an alternating character. 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A method is described for the suppression of the intrinsic (CaMg) endonuclease of rat liver nuclei.</description><subject>Animals</subject><subject>Cell Nucleus - analysis</subject><subject>Cell Nucleus - drug effects</subject><subject>Chickens</subject><subject>Chromatin - analysis</subject><subject>Chromatin - ultrastructure</subject><subject>Deoxyribonuclease I</subject><subject>Deoxyribonucleases - pharmacology</subject><subject>Endonucleases - pharmacology</subject><subject>Erythrocytes - analysis</subject><subject>Liver - analysis</subject><subject>Macromolecular Substances</subject><subject>Nucleosomes - analysis</subject><subject>Rats</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcFu1DAQhi0EKkvhDUCaE4JDip11nIQD0mqhpVIFlyLtzbKdSWOUxIvtbOkz8lJ1dlcrTj1ZM_P_33j0E_KW0QtGmfhEKRVZXrPNh4p9rFnOy2zzjCwYrWmWM8qfk8VJ8pK8CuE3pYxxUZ-RM5ELXrNqQf6tO-8GFe0IYdqiD9FPJk4eP8NthzDi3wg97rAH18JpCEq7HUKcFZPp0QU3IHQqgBpB9RH9OCPvwHTKK5PqC1hBvHfZf3KtAjYQ0FsMYAPc4YhexdTTD7D1Tqe28kOq723s4OuPVTJk15B4M9qNyeT6vWFPta_Ji1b1Ad8c33Py6_Lb7fp7dvPz6nq9uslMXuYxK2heCqqqSjDNS1rwttCN4Q0VChkvWcONLpZty5DrquBlVSVb2xR52-pKK7o8J-8P3PTJPxOGKAcbDPa9GtFNQZZLwepa8CTkB6HxLgSPrdx6Oyj_IBmVc4ZyDkjOAcmKyX2GcpNs7478SafzT6ZjaGn-5TDHdOTOopfBWBwNNtajibJx9ukFj2UDsHM</recordid><startdate>19810415</startdate><enddate>19810415</enddate><creator>Burgoyne, L.A.</creator><creator>Skinner, J.D.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19810415</creationdate><title>Chromatin superstructure: The next level of structure above the nucleosome has an alternating character. 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A two-nucleosome based series is generated by probes armed with DNAase-I acting on isolated nuclei</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1981-04-15</date><risdate>1981</risdate><volume>99</volume><issue>3</issue><spage>893</spage><epage>899</epage><pages>893-899</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Intact nuclei were probed with DNAase-I-based probes and the resultant single strand pieces indicate that many or all of the nucleosomes exist in structures that cause every second nucleosome in a series to be resistant to attack. The effect is observed with chicken erythrocyte nuclei, rat liver nuclei and is weakly elicited by free DNAase-I. 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| fulltext | fulltext |
| identifier | ISSN: 0006-291X |
| ispartof | Biochemical and biophysical research communications, 1981-04, Vol.99 (3), p.893-899 |
| issn | 0006-291X 1090-2104 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Cell Nucleus - analysis Cell Nucleus - drug effects Chickens Chromatin - analysis Chromatin - ultrastructure Deoxyribonuclease I Deoxyribonucleases - pharmacology Endonucleases - pharmacology Erythrocytes - analysis Liver - analysis Macromolecular Substances Nucleosomes - analysis Rats |
| title | Chromatin superstructure: The next level of structure above the nucleosome has an alternating character. A two-nucleosome based series is generated by probes armed with DNAase-I acting on isolated nuclei |
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