Determination of 20-hydroxyeicosatetraenoic acid in microsomal incubates using high-performance liquid chromatography–mass spectrometry (HPLC–MS)

20-HETE is a potent, vasoconstrictive arachidonic acid metabolite with a limited number of published methods for quantitative assessment of microsomal formation rate. The purpose of this study was to evaluate the utility of HPLC–MS (negative ESI) for quantitation of rat microsomal 20-HETE enzyme kin...

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Veröffentlicht in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2003-09, Vol.794 (2), p.363-372
Hauptverfasser: Bolcato, Christopher A, Frye, Reginald F, Zemaitis, Michael A, Poloyac, Samuel M
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Sprache:eng
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Zusammenfassung:20-HETE is a potent, vasoconstrictive arachidonic acid metabolite with a limited number of published methods for quantitative assessment of microsomal formation rate. The purpose of this study was to evaluate the utility of HPLC–MS (negative ESI) for quantitation of rat microsomal 20-HETE enzyme kinetics. Calibration curves were linear over 0.75–16 ng on-column ( r 2>0.996). The intra- and inter-assay precision and accuracy were
ISSN:1570-0232
1873-376X
DOI:10.1016/S1570-0232(03)00496-3