Mechanism of metronidazole-resistance by isolates of nitroreductase-producing Enterococcus gallinarum and Enterococcus casseliflavus from the human intestinal tract
Enterococcus casseliflavus and Enterococcus gallinarum strains resistant to metronidazole, nitrofurantoin and nitrofurazone were isolated from fecal samples of a patient with recurrent ulcerative colitis treated with metronidazole. Unlike other metronidazole-resistant bacteria, these strains produce...
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| Veröffentlicht in: | FEMS microbiology letters 2003-08, Vol.225 (2), p.195-200 |
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| creator | Rafii, Fatemeh Wynne, Rebecca Heinze, Thomas M. Paine, Donald D. |
| description | Enterococcus casseliflavus and
Enterococcus gallinarum strains resistant to metronidazole, nitrofurantoin and nitrofurazone were isolated from fecal samples of a patient with recurrent ulcerative colitis treated with metronidazole. Unlike other metronidazole-resistant bacteria, these strains produced nitroreductase but metabolized metronidazole to compounds that could not be detected by liquid chromatography with UV or mass spectral analysis. Metronidazole-susceptible
Clostridium perfringens grew equally well in spent cultures of
Enterococcus spp. incubated with or without metronidazole. These data indicate that the nitroreductases produced by these
Enterococcus strains did not activate metronidazole to bactericidal metabolites and these bacteria may reduce the effectiveness of metronidazole. We have indirect evidence for an alternative pathway that results in metronidazole resistance. These strains of enterococcus had nitroreductase so resistance should not have occurred. |
| doi_str_mv | 10.1016/S0378-1097(03)00513-5 |
| format | Article |
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Enterococcus gallinarum strains resistant to metronidazole, nitrofurantoin and nitrofurazone were isolated from fecal samples of a patient with recurrent ulcerative colitis treated with metronidazole. Unlike other metronidazole-resistant bacteria, these strains produced nitroreductase but metabolized metronidazole to compounds that could not be detected by liquid chromatography with UV or mass spectral analysis. Metronidazole-susceptible
Clostridium perfringens grew equally well in spent cultures of
Enterococcus spp. incubated with or without metronidazole. These data indicate that the nitroreductases produced by these
Enterococcus strains did not activate metronidazole to bactericidal metabolites and these bacteria may reduce the effectiveness of metronidazole. We have indirect evidence for an alternative pathway that results in metronidazole resistance. These strains of enterococcus had nitroreductase so resistance should not have occurred.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1016/S0378-1097(03)00513-5</identifier><identifier>PMID: 12951241</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Elsevier B.V</publisher><subject>Bacteriology ; Biological and medical sciences ; Chromatography, High Pressure Liquid ; Clostridium perfringens - growth & development ; Drug Resistance, Bacterial ; Enterococcus ; Enterococcus - drug effects ; Enterococcus - growth & development ; Enterococcus - isolation & purification ; Enterococcus - metabolism ; Feces - microbiology ; Fundamental and applied biological sciences. Psychology ; Humans ; Intestinal bacteria ; Intestines - microbiology ; Metronidazole ; Metronidazole - metabolism ; Metronidazole - pharmacology ; Microbiology ; Nitroreductase ; Nitroreductases - biosynthesis ; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains ; Spectrometry, Mass, Electrospray Ionization</subject><ispartof>FEMS microbiology letters, 2003-08, Vol.225 (2), p.195-200</ispartof><rights>2003 Federation of European Microbiological Societies</rights><rights>2003 Federation of European Microbiological Societies 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5135-30c966f5da8df121d0f3525afc40ff0897b6476d9a320cc8dd49a1c48bdeac8a3</citedby><cites>FETCH-LOGICAL-c5135-30c966f5da8df121d0f3525afc40ff0897b6476d9a320cc8dd49a1c48bdeac8a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2FS0378-1097%2803%2900513-5$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1016%2FS0378-1097%2803%2900513-5$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15244603$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12951241$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rafii, Fatemeh</creatorcontrib><creatorcontrib>Wynne, Rebecca</creatorcontrib><creatorcontrib>Heinze, Thomas M.</creatorcontrib><creatorcontrib>Paine, Donald D.</creatorcontrib><title>Mechanism of metronidazole-resistance by isolates of nitroreductase-producing Enterococcus gallinarum and Enterococcus casseliflavus from the human intestinal tract</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>Enterococcus casseliflavus and
Enterococcus gallinarum strains resistant to metronidazole, nitrofurantoin and nitrofurazone were isolated from fecal samples of a patient with recurrent ulcerative colitis treated with metronidazole. Unlike other metronidazole-resistant bacteria, these strains produced nitroreductase but metabolized metronidazole to compounds that could not be detected by liquid chromatography with UV or mass spectral analysis. Metronidazole-susceptible
Clostridium perfringens grew equally well in spent cultures of
Enterococcus spp. incubated with or without metronidazole. These data indicate that the nitroreductases produced by these
Enterococcus strains did not activate metronidazole to bactericidal metabolites and these bacteria may reduce the effectiveness of metronidazole. We have indirect evidence for an alternative pathway that results in metronidazole resistance. These strains of enterococcus had nitroreductase so resistance should not have occurred.</description><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Clostridium perfringens - growth & development</subject><subject>Drug Resistance, Bacterial</subject><subject>Enterococcus</subject><subject>Enterococcus - drug effects</subject><subject>Enterococcus - growth & development</subject><subject>Enterococcus - isolation & purification</subject><subject>Enterococcus - metabolism</subject><subject>Feces - microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Intestinal bacteria</subject><subject>Intestines - microbiology</subject><subject>Metronidazole</subject><subject>Metronidazole - metabolism</subject><subject>Metronidazole - pharmacology</subject><subject>Microbiology</subject><subject>Nitroreductase</subject><subject>Nitroreductases - biosynthesis</subject><subject>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</subject><subject>Spectrometry, Mass, Electrospray Ionization</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkt9qFDEUh4Modq0-gpIbpV6MJpPJ_LkqUloVtnihXoezyUk3kpmsyUxlfZ4-qJnOYikI9SoJ-b6cc_iFkJecveOM1--_MtG0BWddc8LEW8YkF4V8RFZcNlVRd3X7mKz-IkfkWUo_GGNVyeqn5IiXneRlxVfk5hL1FgaXehos7XGMYXAGfgePRcTk0giDRrrZU5eChxHTzA0ucxHNpEdIWOxiyFs3XNHzYcQYdNB6SvQKvHcDxKmnMJj7dxpSQu-sh-t8sjH0dNwi3U49DNRlMo1Z9XSMoMfn5IkFn_DFYT0m3y_Ov519KtZfPn4--7AudJ5eFoLprq6tNNAay0tumBWylGB1xaxlbdds6qqpTQeiZFq3xlQdcF21G4OgWxDH5M3ybh7o55RbUL1LGr2HAcOUVCNqzhvBHwR52zalFGUG5QLqGFKKaNUuuh7iXnGm5hzVbY5qDkkxoW5zVDJ7rw4Fpk2P5s46BJeB1wcAkgZvY47JpTtOllVVM5G5buF-OY_7_6uuLi7XvJubYIsbpt2_zeKeWczK6aJgjunaYVRJO8w_yLiIelQmuAcm_wNbSN3Q</recordid><startdate>20030829</startdate><enddate>20030829</enddate><creator>Rafii, Fatemeh</creator><creator>Wynne, Rebecca</creator><creator>Heinze, Thomas M.</creator><creator>Paine, Donald D.</creator><general>Elsevier B.V</general><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20030829</creationdate><title>Mechanism of metronidazole-resistance by isolates of nitroreductase-producing Enterococcus gallinarum and Enterococcus casseliflavus from the human intestinal tract</title><author>Rafii, Fatemeh ; Wynne, Rebecca ; Heinze, Thomas M. ; Paine, Donald D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5135-30c966f5da8df121d0f3525afc40ff0897b6476d9a320cc8dd49a1c48bdeac8a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Clostridium perfringens - growth & development</topic><topic>Drug Resistance, Bacterial</topic><topic>Enterococcus</topic><topic>Enterococcus - drug effects</topic><topic>Enterococcus - growth & development</topic><topic>Enterococcus - isolation & purification</topic><topic>Enterococcus - metabolism</topic><topic>Feces - microbiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Intestinal bacteria</topic><topic>Intestines - microbiology</topic><topic>Metronidazole</topic><topic>Metronidazole - metabolism</topic><topic>Metronidazole - pharmacology</topic><topic>Microbiology</topic><topic>Nitroreductase</topic><topic>Nitroreductases - biosynthesis</topic><topic>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</topic><topic>Spectrometry, Mass, Electrospray Ionization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rafii, Fatemeh</creatorcontrib><creatorcontrib>Wynne, Rebecca</creatorcontrib><creatorcontrib>Heinze, Thomas M.</creatorcontrib><creatorcontrib>Paine, Donald D.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rafii, Fatemeh</au><au>Wynne, Rebecca</au><au>Heinze, Thomas M.</au><au>Paine, Donald D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mechanism of metronidazole-resistance by isolates of nitroreductase-producing Enterococcus gallinarum and Enterococcus casseliflavus from the human intestinal tract</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>2003-08-29</date><risdate>2003</risdate><volume>225</volume><issue>2</issue><spage>195</spage><epage>200</epage><pages>195-200</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>Enterococcus casseliflavus and
Enterococcus gallinarum strains resistant to metronidazole, nitrofurantoin and nitrofurazone were isolated from fecal samples of a patient with recurrent ulcerative colitis treated with metronidazole. Unlike other metronidazole-resistant bacteria, these strains produced nitroreductase but metabolized metronidazole to compounds that could not be detected by liquid chromatography with UV or mass spectral analysis. Metronidazole-susceptible
Clostridium perfringens grew equally well in spent cultures of
Enterococcus spp. incubated with or without metronidazole. These data indicate that the nitroreductases produced by these
Enterococcus strains did not activate metronidazole to bactericidal metabolites and these bacteria may reduce the effectiveness of metronidazole. We have indirect evidence for an alternative pathway that results in metronidazole resistance. These strains of enterococcus had nitroreductase so resistance should not have occurred.</abstract><cop>Oxford, UK</cop><pub>Elsevier B.V</pub><pmid>12951241</pmid><doi>10.1016/S0378-1097(03)00513-5</doi><tpages>6</tpages></addata></record> |
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| source | MEDLINE; Access via Wiley Online Library; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Bacteriology Biological and medical sciences Chromatography, High Pressure Liquid Clostridium perfringens - growth & development Drug Resistance, Bacterial Enterococcus Enterococcus - drug effects Enterococcus - growth & development Enterococcus - isolation & purification Enterococcus - metabolism Feces - microbiology Fundamental and applied biological sciences. Psychology Humans Intestinal bacteria Intestines - microbiology Metronidazole Metronidazole - metabolism Metronidazole - pharmacology Microbiology Nitroreductase Nitroreductases - biosynthesis Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Spectrometry, Mass, Electrospray Ionization |
| title | Mechanism of metronidazole-resistance by isolates of nitroreductase-producing Enterococcus gallinarum and Enterococcus casseliflavus from the human intestinal tract |
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