Sequence heterogeneity of the merozoite surface protein-1 gene (MSP-1) of Plasmodium vivax wild isolates in southeastern Iran
The merozoite surface protein of Plasmodium vivax (PvMSP-1) has been considered as a vaccine candidate, which exhibits antigenic diversity among isolates. We investigated the extent of sequence variation in the polymorphic region 5 of PvMSP-1 in order to characterize the genetic structure and compos...
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| Veröffentlicht in: | Acta tropica 2003-09, Vol.88 (1), p.91-97 |
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| creator | Zakeri, Sedigheh Dinparast Djadid, Navid Zeinali, Sirous |
| description | The merozoite surface protein of
Plasmodium vivax (PvMSP-1) has been considered as a vaccine candidate, which exhibits antigenic diversity among isolates. We investigated the extent of sequence variation in the polymorphic region 5 of PvMSP-1 in order to characterize the genetic structure and composition of
P. vivax in clinical isolates from Iranshahr and Chahbahar districts of Sistan and Baluchistan province, Iran. The PvMSP-1 gene amplification revealed size variation among the isolates, ranging from 430 to 550 bp. Sequences were obtained for 15 Iranian and one Pakistani isolates and 14 different alleles were detected. Results also showed three distinct sequence types of the polymorphic region. Sequence analysis has shown several single nucleotide polymorphisms to occur in this block of PvMSP-1, creating different alleles in the progeny and also microheterogeneity in the region. Thus, this study provides preliminary evidence of sequence heterogeneity in the Iranian
P. vivax population. |
| doi_str_mv | 10.1016/S0001-706X(03)00192-X |
| format | Article |
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Plasmodium vivax (PvMSP-1) has been considered as a vaccine candidate, which exhibits antigenic diversity among isolates. We investigated the extent of sequence variation in the polymorphic region 5 of PvMSP-1 in order to characterize the genetic structure and composition of
P. vivax in clinical isolates from Iranshahr and Chahbahar districts of Sistan and Baluchistan province, Iran. The PvMSP-1 gene amplification revealed size variation among the isolates, ranging from 430 to 550 bp. Sequences were obtained for 15 Iranian and one Pakistani isolates and 14 different alleles were detected. Results also showed three distinct sequence types of the polymorphic region. Sequence analysis has shown several single nucleotide polymorphisms to occur in this block of PvMSP-1, creating different alleles in the progeny and also microheterogeneity in the region. Thus, this study provides preliminary evidence of sequence heterogeneity in the Iranian
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Plasmodium vivax (PvMSP-1) has been considered as a vaccine candidate, which exhibits antigenic diversity among isolates. We investigated the extent of sequence variation in the polymorphic region 5 of PvMSP-1 in order to characterize the genetic structure and composition of
P. vivax in clinical isolates from Iranshahr and Chahbahar districts of Sistan and Baluchistan province, Iran. The PvMSP-1 gene amplification revealed size variation among the isolates, ranging from 430 to 550 bp. Sequences were obtained for 15 Iranian and one Pakistani isolates and 14 different alleles were detected. Results also showed three distinct sequence types of the polymorphic region. Sequence analysis has shown several single nucleotide polymorphisms to occur in this block of PvMSP-1, creating different alleles in the progeny and also microheterogeneity in the region. Thus, this study provides preliminary evidence of sequence heterogeneity in the Iranian
P. vivax population.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Belem sequence (Bel-1)</subject><subject>Biological and medical sciences</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Human protozoal diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Iran</subject><subject>Malaria</subject><subject>Malaria, Vivax - blood</subject><subject>Medical sciences</subject><subject>Merozoit surface protein-1 (MSP-1)</subject><subject>Merozoite Surface Protein 1 - genetics</subject><subject>Middle Aged</subject><subject>Nucleic Acid Amplification Techniques</subject><subject>Parasitic diseases</subject><subject>Plasmodium vivax</subject><subject>Plasmodium vivax - genetics</subject><subject>Polymerase Chain Reaction</subject><subject>Polymerase chain reaction (PCR)</subject><subject>Protozoal diseases</subject><subject>Salvador 1 sequence (Sal-1)</subject><subject>Sequence type</subject><subject>Tropical medicine</subject><issn>0001-706X</issn><issn>1873-6254</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v1DAQxS0EotvCRwD5QtUeAmM7TpwTqqoClYqotCDtzfI6E2qUxMV2ForEd8fZXdHj-uI_-s288XuEvGLwlgGr3i0BgBU1VKszEOf53PBi9YQsmKpFUXFZPiWL_8gROY7xR77xWvLn5IjxphSNEgvyd4k_Jxwt0jtMGPx3HNGlB-o7mu6QDvnpj3cJaZxCZzJ2H3xCNxaMzig9-7y8Ldj5zN_2Jg6-ddNAN25jftNfrm-pi743CSN1I41-yk1NzEIjvQ5mfEGedaaP-HK_n5BvH66-Xn4qbr58vL68uClsKUUqENZggJdoOCis5LoTSsrK1kxAp1rFZItlK6RAtQYLnNVoTclQAsNGdkKckNNd3zx9_m5MenDRYt-bEf0UdS0qULypDoKCV_XWvEMgU3mxepaWO9AGH2PATt8HN5jwoBnoOUm9TVLPMWkQepukXuW613uBaT1g-1i1jy4Db_aAidb0XbbTuvjIScEbLlnm3u84zAZvHAYdrZsTb11Am3Tr3YFR_gF7cbtU</recordid><startdate>20030901</startdate><enddate>20030901</enddate><creator>Zakeri, Sedigheh</creator><creator>Dinparast Djadid, Navid</creator><creator>Zeinali, Sirous</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>20030901</creationdate><title>Sequence heterogeneity of the merozoite surface protein-1 gene (MSP-1) of Plasmodium vivax wild isolates in southeastern Iran</title><author>Zakeri, Sedigheh ; 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Plasmodium vivax (PvMSP-1) has been considered as a vaccine candidate, which exhibits antigenic diversity among isolates. We investigated the extent of sequence variation in the polymorphic region 5 of PvMSP-1 in order to characterize the genetic structure and composition of
P. vivax in clinical isolates from Iranshahr and Chahbahar districts of Sistan and Baluchistan province, Iran. The PvMSP-1 gene amplification revealed size variation among the isolates, ranging from 430 to 550 bp. Sequences were obtained for 15 Iranian and one Pakistani isolates and 14 different alleles were detected. Results also showed three distinct sequence types of the polymorphic region. Sequence analysis has shown several single nucleotide polymorphisms to occur in this block of PvMSP-1, creating different alleles in the progeny and also microheterogeneity in the region. Thus, this study provides preliminary evidence of sequence heterogeneity in the Iranian
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| subjects | Adolescent Adult Amino Acid Sequence Animals Belem sequence (Bel-1) Biological and medical sciences Child Child, Preschool Human protozoal diseases Humans Infectious diseases Iran Malaria Malaria, Vivax - blood Medical sciences Merozoit surface protein-1 (MSP-1) Merozoite Surface Protein 1 - genetics Middle Aged Nucleic Acid Amplification Techniques Parasitic diseases Plasmodium vivax Plasmodium vivax - genetics Polymerase Chain Reaction Polymerase chain reaction (PCR) Protozoal diseases Salvador 1 sequence (Sal-1) Sequence type Tropical medicine |
| title | Sequence heterogeneity of the merozoite surface protein-1 gene (MSP-1) of Plasmodium vivax wild isolates in southeastern Iran |
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