New type of snRNP containing nuclear bodies in plant cells
In larch ( Larix decidua Mill.) microspores a new type of nuclear bodies has been found which are an element of the spatial organization of the splicing system in plant cell. These are bizonal bodies, ultrastructurally differentiated into a coiled part and a dense part. Using immunocytochemistry and...
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| Veröffentlicht in: | Biology of the cell 2003-07, Vol.95 (5), p.303-310 |
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| creator | Niedojadleo, J Gorska-Brylass, A |
| description | In larch (
Larix decidua Mill.) microspores a new type of nuclear bodies has been found which are an element of the spatial organization of the splicing system in plant cell. These are bizonal bodies, ultrastructurally differentiated into a coiled part and a dense part. Using immunocytochemistry and in situ hybridization at the EM level, the coiled part of the bizonal body was found to contain snRNA including U2 snRNA, Sm proteins and nucleolar proteins of the agyrophilic type and fibrillarin. The dense part contains Sm proteins but lacks snRNA. Such a separation of macromolecules related to splicing occurring within the bizonal bodies microspore is striking by the similarity of these bodies to amphibian oocyte snurposomes. The occurrence in plant cells, beside widely known coiled bodies (CBs), also of other nuclear bodies related to splicing proves that in plants similarly as for animals the differentiation among domains containing elements of the splicing system occurs. |
| doi_str_mv | 10.1016/S0248-4900(03)00061-3 |
| format | Article |
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Larix decidua Mill.) microspores a new type of nuclear bodies has been found which are an element of the spatial organization of the splicing system in plant cell. These are bizonal bodies, ultrastructurally differentiated into a coiled part and a dense part. Using immunocytochemistry and in situ hybridization at the EM level, the coiled part of the bizonal body was found to contain snRNA including U2 snRNA, Sm proteins and nucleolar proteins of the agyrophilic type and fibrillarin. The dense part contains Sm proteins but lacks snRNA. Such a separation of macromolecules related to splicing occurring within the bizonal bodies microspore is striking by the similarity of these bodies to amphibian oocyte snurposomes. The occurrence in plant cells, beside widely known coiled bodies (CBs), also of other nuclear bodies related to splicing proves that in plants similarly as for animals the differentiation among domains containing elements of the splicing system occurs.</description><identifier>ISSN: 0248-4900</identifier><identifier>EISSN: 1768-322X</identifier><identifier>DOI: 10.1016/S0248-4900(03)00061-3</identifier><identifier>PMID: 12941528</identifier><language>eng</language><publisher>Oxford, UK: Elsevier SAS</publisher><subject>ASm protein ; Bizonal body ; Cell Nucleolus - chemistry ; Cell Nucleus - ultrastructure ; Chromosomal Proteins, Non-Histone - analysis ; Coiled Bodies - chemistry ; Coiled Bodies - ultrastructure ; Coiled body ; DNA - analysis ; In Situ Hybridization - methods ; Interphase ; Intranuclear Space - chemistry ; Larix - cytology ; Larix - ultrastructure ; Larix decidua ; Microscopy, Immunoelectron - methods ; Microspore ; Pollen - cytology ; Pollen - ultrastructure ; Ribonucleoproteins (small nuclear) ; Ribonucleoproteins, Small Nuclear - analysis ; Ribonucleoproteins, Small Nuclear - genetics ; Ribonucleoproteins, Small Nuclear - physiology ; RNA Splicing ; RNA, Ribosomal, 18S - analysis ; RNA, Ribosomal, 18S - genetics ; Splicing</subject><ispartof>Biology of the cell, 2003-07, Vol.95 (5), p.303-310</ispartof><rights>2003 Éditions scientifiques et médicales Elsevier SAS</rights><rights>2003 Société Française des Microscopies and Société Biologie Cellulaire de France</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4817-3867c45368331e7d2db2f26fb2da49ac65d121100405053aa19f2d24648a7a8c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2FS0248-4900%2803%2900061-3$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1016%2FS0248-4900%2803%2900061-3$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12941528$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Niedojadleo, J</creatorcontrib><creatorcontrib>Gorska-Brylass, A</creatorcontrib><title>New type of snRNP containing nuclear bodies in plant cells</title><title>Biology of the cell</title><addtitle>Biol Cell</addtitle><description>In larch (
Larix decidua Mill.) microspores a new type of nuclear bodies has been found which are an element of the spatial organization of the splicing system in plant cell. These are bizonal bodies, ultrastructurally differentiated into a coiled part and a dense part. Using immunocytochemistry and in situ hybridization at the EM level, the coiled part of the bizonal body was found to contain snRNA including U2 snRNA, Sm proteins and nucleolar proteins of the agyrophilic type and fibrillarin. The dense part contains Sm proteins but lacks snRNA. Such a separation of macromolecules related to splicing occurring within the bizonal bodies microspore is striking by the similarity of these bodies to amphibian oocyte snurposomes. The occurrence in plant cells, beside widely known coiled bodies (CBs), also of other nuclear bodies related to splicing proves that in plants similarly as for animals the differentiation among domains containing elements of the splicing system occurs.</description><subject>ASm protein</subject><subject>Bizonal body</subject><subject>Cell Nucleolus - chemistry</subject><subject>Cell Nucleus - ultrastructure</subject><subject>Chromosomal Proteins, Non-Histone - analysis</subject><subject>Coiled Bodies - chemistry</subject><subject>Coiled Bodies - ultrastructure</subject><subject>Coiled body</subject><subject>DNA - analysis</subject><subject>In Situ Hybridization - methods</subject><subject>Interphase</subject><subject>Intranuclear Space - chemistry</subject><subject>Larix - cytology</subject><subject>Larix - ultrastructure</subject><subject>Larix decidua</subject><subject>Microscopy, Immunoelectron - methods</subject><subject>Microspore</subject><subject>Pollen - cytology</subject><subject>Pollen - ultrastructure</subject><subject>Ribonucleoproteins (small nuclear)</subject><subject>Ribonucleoproteins, Small Nuclear - analysis</subject><subject>Ribonucleoproteins, Small Nuclear - genetics</subject><subject>Ribonucleoproteins, Small Nuclear - physiology</subject><subject>RNA Splicing</subject><subject>RNA, Ribosomal, 18S - analysis</subject><subject>RNA, Ribosomal, 18S - genetics</subject><subject>Splicing</subject><issn>0248-4900</issn><issn>1768-322X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkMtuEzEUQC0EoqHwCSCvECyG-v3oBkFEH1KVAgXBznLsO8gw8QR7Qsnfd9KJyrKsvDnn3uuD0HNK3lBC1dEVYcI0whLyivDXhBBFG_4AzahWpuGMfX-IZnfIAXpS688REtbIx-iAMiuoZGaGjhdwjYftGnDf4po_Lz7i0OfBp5zyD5w3oQNf8LKPCSpOGa87nwccoOvqU_So9V2FZ_v3EH09-fBlftZcXJ6ez99dNEEYqhtulA5CcmU4p6Aji0vWMtUuWfTC-qBkpIzS8TYiieTeU9uyyIQSxmtvAj9EL6e569L_3kAd3CrV3QU-Q7-pTnNFpDL2XpBaJqS1bATlBIbS11qgdeuSVr5sHSVuV9fd1nW7dI5wd1vX8dF7sV-wWa4g_rP2OUfgeAKuUwfb_5vq3l_OqTZ6lJtJTnWAv3eyL7-c0lxL921x6gS9-mTOFHFi5N9OPIzx_yQoroYEOUBMBcLgYp_u-c8N43umew</recordid><startdate>200307</startdate><enddate>200307</enddate><creator>Niedojadleo, J</creator><creator>Gorska-Brylass, A</creator><general>Elsevier SAS</general><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>200307</creationdate><title>New type of snRNP containing nuclear bodies in plant cells</title><author>Niedojadleo, J ; Gorska-Brylass, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4817-3867c45368331e7d2db2f26fb2da49ac65d121100405053aa19f2d24648a7a8c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>ASm protein</topic><topic>Bizonal body</topic><topic>Cell Nucleolus - chemistry</topic><topic>Cell Nucleus - ultrastructure</topic><topic>Chromosomal Proteins, Non-Histone - analysis</topic><topic>Coiled Bodies - chemistry</topic><topic>Coiled Bodies - ultrastructure</topic><topic>Coiled body</topic><topic>DNA - analysis</topic><topic>In Situ Hybridization - methods</topic><topic>Interphase</topic><topic>Intranuclear Space - chemistry</topic><topic>Larix - cytology</topic><topic>Larix - ultrastructure</topic><topic>Larix decidua</topic><topic>Microscopy, Immunoelectron - methods</topic><topic>Microspore</topic><topic>Pollen - cytology</topic><topic>Pollen - ultrastructure</topic><topic>Ribonucleoproteins (small nuclear)</topic><topic>Ribonucleoproteins, Small Nuclear - analysis</topic><topic>Ribonucleoproteins, Small Nuclear - genetics</topic><topic>Ribonucleoproteins, Small Nuclear - physiology</topic><topic>RNA Splicing</topic><topic>RNA, Ribosomal, 18S - analysis</topic><topic>RNA, Ribosomal, 18S - genetics</topic><topic>Splicing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Niedojadleo, J</creatorcontrib><creatorcontrib>Gorska-Brylass, A</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of the cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Niedojadleo, J</au><au>Gorska-Brylass, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>New type of snRNP containing nuclear bodies in plant cells</atitle><jtitle>Biology of the cell</jtitle><addtitle>Biol Cell</addtitle><date>2003-07</date><risdate>2003</risdate><volume>95</volume><issue>5</issue><spage>303</spage><epage>310</epage><pages>303-310</pages><issn>0248-4900</issn><eissn>1768-322X</eissn><abstract>In larch (
Larix decidua Mill.) microspores a new type of nuclear bodies has been found which are an element of the spatial organization of the splicing system in plant cell. These are bizonal bodies, ultrastructurally differentiated into a coiled part and a dense part. Using immunocytochemistry and in situ hybridization at the EM level, the coiled part of the bizonal body was found to contain snRNA including U2 snRNA, Sm proteins and nucleolar proteins of the agyrophilic type and fibrillarin. The dense part contains Sm proteins but lacks snRNA. Such a separation of macromolecules related to splicing occurring within the bizonal bodies microspore is striking by the similarity of these bodies to amphibian oocyte snurposomes. The occurrence in plant cells, beside widely known coiled bodies (CBs), also of other nuclear bodies related to splicing proves that in plants similarly as for animals the differentiation among domains containing elements of the splicing system occurs.</abstract><cop>Oxford, UK</cop><pub>Elsevier SAS</pub><pmid>12941528</pmid><doi>10.1016/S0248-4900(03)00061-3</doi><tpages>8</tpages></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Alma/SFX Local Collection |
| subjects | ASm protein Bizonal body Cell Nucleolus - chemistry Cell Nucleus - ultrastructure Chromosomal Proteins, Non-Histone - analysis Coiled Bodies - chemistry Coiled Bodies - ultrastructure Coiled body DNA - analysis In Situ Hybridization - methods Interphase Intranuclear Space - chemistry Larix - cytology Larix - ultrastructure Larix decidua Microscopy, Immunoelectron - methods Microspore Pollen - cytology Pollen - ultrastructure Ribonucleoproteins (small nuclear) Ribonucleoproteins, Small Nuclear - analysis Ribonucleoproteins, Small Nuclear - genetics Ribonucleoproteins, Small Nuclear - physiology RNA Splicing RNA, Ribosomal, 18S - analysis RNA, Ribosomal, 18S - genetics Splicing |
| title | New type of snRNP containing nuclear bodies in plant cells |
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