Identification of Hypothalamic Nuclei Involved in the Orexigenic Effect of Melanin-Concentrating Hormone
The hypothalamic neuropeptide melanin-concentrating hormone (MCH) increases feeding when injected intracerebroventricularly in rats. To identify the hypothalamic nuclei responsible for the orexigenic effect, we injected the peptide into discrete hypothalamic nuclei known to express the MCH receptor,...
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| Veröffentlicht in: | Endocrinology (Philadelphia) 2003-09, Vol.144 (9), p.3943-3949 |
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| description | The hypothalamic neuropeptide melanin-concentrating hormone (MCH) increases feeding when injected intracerebroventricularly in rats. To identify the hypothalamic nuclei responsible for the orexigenic effect, we injected the peptide into discrete hypothalamic nuclei known to express the MCH receptor, MCH1R. MCH (0.6 nmol) elicited a rapid and significant increase in feeding in satiated rats following injection into the arcuate nucleus (0–1 h: 421 ± 60%; P < 0.01). An elevation in feeding was also observed following injection into the paraventricular nucleus, which was sustained up to 4 h post injection (0–4 h: 218 ± 29%; P < 0.01). A significant increase in feeding during this time period was also observed following injection into the dorsomedial nucleus (0–4 h: 155 ± 12%; P < 0.05). No significant alteration in feeding was observed following injection into the supraoptic nucleus, lateral hypothalamic area, medial preoptic area, anterior hypothalamic area, or ventromedial nucleus of the hypothalamus. To identify the neurotransmitters that may be potentially involved in this effect, we examined their release from hypothalamic explants in vitro following exogenous MCH administration. MCH (1 μm) increased the release of the orexigenic neurotransmitters neuropeptide Y (37.8 ± 6.0 fmol/explant vs. basal 30.2 ± 4.3 fmol/explant; P < 0.05) and agouti-related peptide (4.1 ± 0.6 fmol/explant vs. basal 2.4 ± 0.2 fmol/explant; P < 0.05) and decreased the release of the anorectic neurotransmitters α-MSH (41.7 ± 6.8 fmol/explant vs. basal 65.9 ± 11.0 fmol/explant; P < 0.01) and cocaine- and amphetamine-regulated transcript (112.3 ± 12.4 fmol/explant vs. basal 167.4 ± 13.0 fmol/explant; P < 0.001). These studies suggest that the orexigenic effect of MCH may be mediated via activation or inhibition of these feeding circuits within the arcuate nucleus and paraventricular nucleus of the hypothalamus. |
| doi_str_mv | 10.1210/en.2003-0149 |
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To identify the hypothalamic nuclei responsible for the orexigenic effect, we injected the peptide into discrete hypothalamic nuclei known to express the MCH receptor, MCH1R. MCH (0.6 nmol) elicited a rapid and significant increase in feeding in satiated rats following injection into the arcuate nucleus (0–1 h: 421 ± 60%; P < 0.01). An elevation in feeding was also observed following injection into the paraventricular nucleus, which was sustained up to 4 h post injection (0–4 h: 218 ± 29%; P < 0.01). A significant increase in feeding during this time period was also observed following injection into the dorsomedial nucleus (0–4 h: 155 ± 12%; P < 0.05). No significant alteration in feeding was observed following injection into the supraoptic nucleus, lateral hypothalamic area, medial preoptic area, anterior hypothalamic area, or ventromedial nucleus of the hypothalamus. To identify the neurotransmitters that may be potentially involved in this effect, we examined their release from hypothalamic explants in vitro following exogenous MCH administration. MCH (1 μm) increased the release of the orexigenic neurotransmitters neuropeptide Y (37.8 ± 6.0 fmol/explant vs. basal 30.2 ± 4.3 fmol/explant; P < 0.05) and agouti-related peptide (4.1 ± 0.6 fmol/explant vs. basal 2.4 ± 0.2 fmol/explant; P < 0.05) and decreased the release of the anorectic neurotransmitters α-MSH (41.7 ± 6.8 fmol/explant vs. basal 65.9 ± 11.0 fmol/explant; P < 0.01) and cocaine- and amphetamine-regulated transcript (112.3 ± 12.4 fmol/explant vs. basal 167.4 ± 13.0 fmol/explant; P < 0.001). These studies suggest that the orexigenic effect of MCH may be mediated via activation or inhibition of these feeding circuits within the arcuate nucleus and paraventricular nucleus of the hypothalamus.]]></description><identifier>ISSN: 0013-7227</identifier><identifier>EISSN: 1945-7170</identifier><identifier>DOI: 10.1210/en.2003-0149</identifier><identifier>PMID: 12933668</identifier><identifier>CODEN: ENDOAO</identifier><language>eng</language><publisher>Bethesda, MD: Endocrine Society</publisher><subject>Agouti-Related Protein ; alpha-MSH - metabolism ; Amphetamines ; Animals ; Appetite - drug effects ; Appetite - physiology ; Arcuate nucleus ; Arcuate Nucleus of Hypothalamus - drug effects ; Arcuate Nucleus of Hypothalamus - metabolism ; Biological and medical sciences ; Cocaine ; Cocaine- and amphetamine-regulated transcript protein ; Eating - drug effects ; Eating - physiology ; Explants ; Feeding ; Feeding Behavior - drug effects ; Feeding Behavior - physiology ; Fundamental and applied biological sciences. Psychology ; Hormones and neuropeptides. Regulation ; Hypothalamic Hormones - pharmacology ; Hypothalamus ; Hypothalamus (anterior) ; Hypothalamus (lateral) ; Hypothalamus (medial) ; Hypothalamus (ventromedial) ; Hypothalamus. Hypophysis. Epiphysis. Urophysis ; Injection ; Intercellular Signaling Peptides and Proteins ; Male ; Melanin ; Melanin-concentrating hormone ; Melanins - pharmacology ; Microinjections ; Neuropeptide Y ; Neuropeptide Y - metabolism ; Neuropeptides ; Neurotransmitters ; Nuclei ; Paraventricular Hypothalamic Nucleus - drug effects ; Paraventricular Hypothalamic Nucleus - metabolism ; Paraventricular nucleus ; Peptides ; Pituitary Hormones - pharmacology ; Preoptic area ; Preoptic area (medial) ; Proteins - metabolism ; Rats ; Rats, Wistar ; Supraoptic nucleus ; Vertebrates: endocrinology</subject><ispartof>Endocrinology (Philadelphia), 2003-09, Vol.144 (9), p.3943-3949</ispartof><rights>Copyright © 2003 by The Endocrine Society 2003</rights><rights>2004 INIST-CNRS</rights><rights>Copyright © 2003 by The Endocrine Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c525t-d86fdee8034204426df27628e1cfa0e9e7bc93ec3cc3bd3236095ec674344fc53</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15064639$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12933668$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Abbott, Caroline R</creatorcontrib><creatorcontrib>Kennedy, Adam R</creatorcontrib><creatorcontrib>Wren, Alison M</creatorcontrib><creatorcontrib>Rossi, Michela</creatorcontrib><creatorcontrib>Murphy, Kevin G</creatorcontrib><creatorcontrib>Seal, Leighton J</creatorcontrib><creatorcontrib>Todd, Jeannie F</creatorcontrib><creatorcontrib>Ghatei, Mohammad A</creatorcontrib><creatorcontrib>Small, Caroline J</creatorcontrib><creatorcontrib>Bloom, Stephen R</creatorcontrib><title>Identification of Hypothalamic Nuclei Involved in the Orexigenic Effect of Melanin-Concentrating Hormone</title><title>Endocrinology (Philadelphia)</title><addtitle>Endocrinology</addtitle><description><![CDATA[The hypothalamic neuropeptide melanin-concentrating hormone (MCH) increases feeding when injected intracerebroventricularly in rats. To identify the hypothalamic nuclei responsible for the orexigenic effect, we injected the peptide into discrete hypothalamic nuclei known to express the MCH receptor, MCH1R. MCH (0.6 nmol) elicited a rapid and significant increase in feeding in satiated rats following injection into the arcuate nucleus (0–1 h: 421 ± 60%; P < 0.01). An elevation in feeding was also observed following injection into the paraventricular nucleus, which was sustained up to 4 h post injection (0–4 h: 218 ± 29%; P < 0.01). A significant increase in feeding during this time period was also observed following injection into the dorsomedial nucleus (0–4 h: 155 ± 12%; P < 0.05). No significant alteration in feeding was observed following injection into the supraoptic nucleus, lateral hypothalamic area, medial preoptic area, anterior hypothalamic area, or ventromedial nucleus of the hypothalamus. To identify the neurotransmitters that may be potentially involved in this effect, we examined their release from hypothalamic explants in vitro following exogenous MCH administration. MCH (1 μm) increased the release of the orexigenic neurotransmitters neuropeptide Y (37.8 ± 6.0 fmol/explant vs. basal 30.2 ± 4.3 fmol/explant; P < 0.05) and agouti-related peptide (4.1 ± 0.6 fmol/explant vs. basal 2.4 ± 0.2 fmol/explant; P < 0.05) and decreased the release of the anorectic neurotransmitters α-MSH (41.7 ± 6.8 fmol/explant vs. basal 65.9 ± 11.0 fmol/explant; P < 0.01) and cocaine- and amphetamine-regulated transcript (112.3 ± 12.4 fmol/explant vs. basal 167.4 ± 13.0 fmol/explant; P < 0.001). These studies suggest that the orexigenic effect of MCH may be mediated via activation or inhibition of these feeding circuits within the arcuate nucleus and paraventricular nucleus of the hypothalamus.]]></description><subject>Agouti-Related Protein</subject><subject>alpha-MSH - metabolism</subject><subject>Amphetamines</subject><subject>Animals</subject><subject>Appetite - drug effects</subject><subject>Appetite - physiology</subject><subject>Arcuate nucleus</subject><subject>Arcuate Nucleus of Hypothalamus - drug effects</subject><subject>Arcuate Nucleus of Hypothalamus - metabolism</subject><subject>Biological and medical sciences</subject><subject>Cocaine</subject><subject>Cocaine- and amphetamine-regulated transcript protein</subject><subject>Eating - drug effects</subject><subject>Eating - physiology</subject><subject>Explants</subject><subject>Feeding</subject><subject>Feeding Behavior - drug effects</subject><subject>Feeding Behavior - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hormones and neuropeptides. Regulation</subject><subject>Hypothalamic Hormones - pharmacology</subject><subject>Hypothalamus</subject><subject>Hypothalamus (anterior)</subject><subject>Hypothalamus (lateral)</subject><subject>Hypothalamus (medial)</subject><subject>Hypothalamus (ventromedial)</subject><subject>Hypothalamus. Hypophysis. Epiphysis. Urophysis</subject><subject>Injection</subject><subject>Intercellular Signaling Peptides and Proteins</subject><subject>Male</subject><subject>Melanin</subject><subject>Melanin-concentrating hormone</subject><subject>Melanins - pharmacology</subject><subject>Microinjections</subject><subject>Neuropeptide Y</subject><subject>Neuropeptide Y - metabolism</subject><subject>Neuropeptides</subject><subject>Neurotransmitters</subject><subject>Nuclei</subject><subject>Paraventricular Hypothalamic Nucleus - drug effects</subject><subject>Paraventricular Hypothalamic Nucleus - metabolism</subject><subject>Paraventricular nucleus</subject><subject>Peptides</subject><subject>Pituitary Hormones - pharmacology</subject><subject>Preoptic area</subject><subject>Preoptic area (medial)</subject><subject>Proteins - metabolism</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Supraoptic nucleus</subject><subject>Vertebrates: endocrinology</subject><issn>0013-7227</issn><issn>1945-7170</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10Etr3DAUBWAREpJp0l3XxRDabupUL9vjZRnSzkAem2QtNNdXGQVbciU7JP8-MmMYKO1KCD6de3UI-cToFeOM_kB3xSkVOWWyPiILVssir1hFj8mCUibyivPqjHyI8TldpZTilJwxXgtRlssF2W0adIM1FvRgvcu8ydZvvR92utWdhexuhBZttnEvvn3BJrMuG3aY3Qd8tU_okrg2BmGYHt5iq511-co7SKEhJbqnbO1D5x1ekBOj24gf5_OcPP66flit85v735vVz5scCl4MebMsTYO4pEJyKiUvG8Orki-RgdEUa6y2UAsEASC2jeCipHWBUFZSSGmgEOfk6z63D_7PiHFQnY2AbVoN_RhVlV5QxkWCl3_BZz8Gl3ZTgglapH5kmdT3vYLgYwxoVB9sp8ObYlRN_St0aupfTf0n_nkOHbcdNgc8F57AlxnoCLo1QTuw8eAKOk2dgr7tnR_7_43M55FiL9E1HoJ12AeM8fCbfy76DklfqlM</recordid><startdate>20030901</startdate><enddate>20030901</enddate><creator>Abbott, Caroline R</creator><creator>Kennedy, Adam R</creator><creator>Wren, Alison M</creator><creator>Rossi, Michela</creator><creator>Murphy, Kevin G</creator><creator>Seal, Leighton J</creator><creator>Todd, Jeannie F</creator><creator>Ghatei, Mohammad A</creator><creator>Small, Caroline J</creator><creator>Bloom, Stephen R</creator><general>Endocrine Society</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20030901</creationdate><title>Identification of Hypothalamic Nuclei Involved in the Orexigenic Effect of Melanin-Concentrating Hormone</title><author>Abbott, Caroline R ; Kennedy, Adam R ; Wren, Alison M ; Rossi, Michela ; Murphy, Kevin G ; Seal, Leighton J ; Todd, Jeannie F ; Ghatei, Mohammad A ; Small, Caroline J ; Bloom, Stephen R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c525t-d86fdee8034204426df27628e1cfa0e9e7bc93ec3cc3bd3236095ec674344fc53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Agouti-Related Protein</topic><topic>alpha-MSH - metabolism</topic><topic>Amphetamines</topic><topic>Animals</topic><topic>Appetite - drug effects</topic><topic>Appetite - physiology</topic><topic>Arcuate nucleus</topic><topic>Arcuate Nucleus of Hypothalamus - drug effects</topic><topic>Arcuate Nucleus of Hypothalamus - metabolism</topic><topic>Biological and medical sciences</topic><topic>Cocaine</topic><topic>Cocaine- and amphetamine-regulated transcript protein</topic><topic>Eating - drug effects</topic><topic>Eating - physiology</topic><topic>Explants</topic><topic>Feeding</topic><topic>Feeding Behavior - drug effects</topic><topic>Feeding Behavior - physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hormones and neuropeptides. Regulation</topic><topic>Hypothalamic Hormones - pharmacology</topic><topic>Hypothalamus</topic><topic>Hypothalamus (anterior)</topic><topic>Hypothalamus (lateral)</topic><topic>Hypothalamus (medial)</topic><topic>Hypothalamus (ventromedial)</topic><topic>Hypothalamus. Hypophysis. Epiphysis. Urophysis</topic><topic>Injection</topic><topic>Intercellular Signaling Peptides and Proteins</topic><topic>Male</topic><topic>Melanin</topic><topic>Melanin-concentrating hormone</topic><topic>Melanins - pharmacology</topic><topic>Microinjections</topic><topic>Neuropeptide Y</topic><topic>Neuropeptide Y - metabolism</topic><topic>Neuropeptides</topic><topic>Neurotransmitters</topic><topic>Nuclei</topic><topic>Paraventricular Hypothalamic Nucleus - drug effects</topic><topic>Paraventricular Hypothalamic Nucleus - metabolism</topic><topic>Paraventricular nucleus</topic><topic>Peptides</topic><topic>Pituitary Hormones - pharmacology</topic><topic>Preoptic area</topic><topic>Preoptic area (medial)</topic><topic>Proteins - metabolism</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Supraoptic nucleus</topic><topic>Vertebrates: endocrinology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Abbott, Caroline R</creatorcontrib><creatorcontrib>Kennedy, Adam R</creatorcontrib><creatorcontrib>Wren, Alison M</creatorcontrib><creatorcontrib>Rossi, Michela</creatorcontrib><creatorcontrib>Murphy, Kevin G</creatorcontrib><creatorcontrib>Seal, Leighton J</creatorcontrib><creatorcontrib>Todd, Jeannie F</creatorcontrib><creatorcontrib>Ghatei, Mohammad A</creatorcontrib><creatorcontrib>Small, Caroline J</creatorcontrib><creatorcontrib>Bloom, Stephen R</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Endocrinology (Philadelphia)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abbott, Caroline R</au><au>Kennedy, Adam R</au><au>Wren, Alison M</au><au>Rossi, Michela</au><au>Murphy, Kevin G</au><au>Seal, Leighton J</au><au>Todd, Jeannie F</au><au>Ghatei, Mohammad A</au><au>Small, Caroline J</au><au>Bloom, Stephen R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of Hypothalamic Nuclei Involved in the Orexigenic Effect of Melanin-Concentrating Hormone</atitle><jtitle>Endocrinology (Philadelphia)</jtitle><addtitle>Endocrinology</addtitle><date>2003-09-01</date><risdate>2003</risdate><volume>144</volume><issue>9</issue><spage>3943</spage><epage>3949</epage><pages>3943-3949</pages><issn>0013-7227</issn><eissn>1945-7170</eissn><coden>ENDOAO</coden><abstract><![CDATA[The hypothalamic neuropeptide melanin-concentrating hormone (MCH) increases feeding when injected intracerebroventricularly in rats. To identify the hypothalamic nuclei responsible for the orexigenic effect, we injected the peptide into discrete hypothalamic nuclei known to express the MCH receptor, MCH1R. MCH (0.6 nmol) elicited a rapid and significant increase in feeding in satiated rats following injection into the arcuate nucleus (0–1 h: 421 ± 60%; P < 0.01). An elevation in feeding was also observed following injection into the paraventricular nucleus, which was sustained up to 4 h post injection (0–4 h: 218 ± 29%; P < 0.01). A significant increase in feeding during this time period was also observed following injection into the dorsomedial nucleus (0–4 h: 155 ± 12%; P < 0.05). No significant alteration in feeding was observed following injection into the supraoptic nucleus, lateral hypothalamic area, medial preoptic area, anterior hypothalamic area, or ventromedial nucleus of the hypothalamus. To identify the neurotransmitters that may be potentially involved in this effect, we examined their release from hypothalamic explants in vitro following exogenous MCH administration. MCH (1 μm) increased the release of the orexigenic neurotransmitters neuropeptide Y (37.8 ± 6.0 fmol/explant vs. basal 30.2 ± 4.3 fmol/explant; P < 0.05) and agouti-related peptide (4.1 ± 0.6 fmol/explant vs. basal 2.4 ± 0.2 fmol/explant; P < 0.05) and decreased the release of the anorectic neurotransmitters α-MSH (41.7 ± 6.8 fmol/explant vs. basal 65.9 ± 11.0 fmol/explant; P < 0.01) and cocaine- and amphetamine-regulated transcript (112.3 ± 12.4 fmol/explant vs. basal 167.4 ± 13.0 fmol/explant; P < 0.001). These studies suggest that the orexigenic effect of MCH may be mediated via activation or inhibition of these feeding circuits within the arcuate nucleus and paraventricular nucleus of the hypothalamus.]]></abstract><cop>Bethesda, MD</cop><pub>Endocrine Society</pub><pmid>12933668</pmid><doi>10.1210/en.2003-0149</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals |
| subjects | Agouti-Related Protein alpha-MSH - metabolism Amphetamines Animals Appetite - drug effects Appetite - physiology Arcuate nucleus Arcuate Nucleus of Hypothalamus - drug effects Arcuate Nucleus of Hypothalamus - metabolism Biological and medical sciences Cocaine Cocaine- and amphetamine-regulated transcript protein Eating - drug effects Eating - physiology Explants Feeding Feeding Behavior - drug effects Feeding Behavior - physiology Fundamental and applied biological sciences. Psychology Hormones and neuropeptides. Regulation Hypothalamic Hormones - pharmacology Hypothalamus Hypothalamus (anterior) Hypothalamus (lateral) Hypothalamus (medial) Hypothalamus (ventromedial) Hypothalamus. Hypophysis. Epiphysis. Urophysis Injection Intercellular Signaling Peptides and Proteins Male Melanin Melanin-concentrating hormone Melanins - pharmacology Microinjections Neuropeptide Y Neuropeptide Y - metabolism Neuropeptides Neurotransmitters Nuclei Paraventricular Hypothalamic Nucleus - drug effects Paraventricular Hypothalamic Nucleus - metabolism Paraventricular nucleus Peptides Pituitary Hormones - pharmacology Preoptic area Preoptic area (medial) Proteins - metabolism Rats Rats, Wistar Supraoptic nucleus Vertebrates: endocrinology |
| title | Identification of Hypothalamic Nuclei Involved in the Orexigenic Effect of Melanin-Concentrating Hormone |
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