Vitellogenin expression in zebrafish Danio rerio: evaluation by histochemistry, immunohistochemistry, and in situ mRNA hybridisation

Several histological methods were tested for their potential to detect the in vivo induction of vitellogenin in zebrafish, after exposure to 17β-oestradiol (E2), and validated by correlating semi-quantitative measurements on digital images to vitellogenin plasma values measured by ELISA and morpholo...

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Veröffentlicht in:	Aquatic toxicology 2003-10, Vol.65 (1), p.1-11
Hauptverfasser:	Van der Ven, Leo T.M., Holbech, Henrik, Fenske, Martina, Van den Brandhof, Evert-Jan, Gielis-Proper, Francijntje K., Wester, Piet W.
Format:	Artikel
Sprache:	eng
Schlagworte:	17β-oestradiol Analytical Methods Animal, plant and microbial ecology Animals Applied ecology Biological and medical sciences Danio rerio Ecotoxicology, biological effects of pollution Endocrine disruption Enzyme-Linked Immunosorbent Assay Estradiol - pharmacology Freshwater Fundamental and applied biological sciences. Psychology Gene Expression - drug effects Gene Expression - genetics Hazard identification Immunohistochemistry In Situ Hybridization In situ mRNA hybridisation Liver - metabolism Myocardium - metabolism RNA, Messenger - genetics Techniques Teleost fish Vitellogenin Vitellogenins - biosynthesis Vitellogenins - genetics Zebrafish ( Danio rerio) Zebrafish - genetics
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