Synthesis and Characterization of Thermoreversible Biopolymer Microgels Based on Hydrogen Bonded Nucleobase Pairing
We describe the synthesis and characterization of a thermoreversibly cross-linked biopolymer microgel based on protein, DNA, and peptide nucleic acid (PNA) components. The DNA assembles into a trifunctional three-way junction (TWJ) with single-stranded overhangs. PNA oligomers complementary to these...
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| Veröffentlicht in: | Journal of the American Chemical Society 2003-08, Vol.125 (34), p.10250-10256 |
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| container_issue | 34 |
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| container_title | Journal of the American Chemical Society |
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| creator | Cao, Rong Gu, Zhenyu Hsu, Lorraine Patterson, Gary D Armitage, Bruce A |
| description | We describe the synthesis and characterization of a thermoreversibly cross-linked biopolymer microgel based on protein, DNA, and peptide nucleic acid (PNA) components. The DNA assembles into a trifunctional three-way junction (TWJ) with single-stranded overhangs. PNA oligomers complementary to these overhangs and bearing terminal biotin groups hybridize to the DNA TWJ and simultaneously bind to the tetrafunctional protein avidin, leading to a cross-linked system. Dynamic light scattering experiments reveal that micron-sized particles are formed. Static light scattering was used to characterize the internal structure of these microgels, which were found to have a fractal dimension of 1.85, indicative of a loose network structure. Heating disrupts the weakest component in the system, namely the PNA−DNA hybrid, resulting in dissolution of the microgel, while cooling restores the hydrogen bonding leading to reassembly of the microgel. Variation of the nucleotide sequence permits tuning of the gelation temperature with fine control. |
| doi_str_mv | 10.1021/ja035211t |
| format | Article |
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The DNA assembles into a trifunctional three-way junction (TWJ) with single-stranded overhangs. PNA oligomers complementary to these overhangs and bearing terminal biotin groups hybridize to the DNA TWJ and simultaneously bind to the tetrafunctional protein avidin, leading to a cross-linked system. Dynamic light scattering experiments reveal that micron-sized particles are formed. Static light scattering was used to characterize the internal structure of these microgels, which were found to have a fractal dimension of 1.85, indicative of a loose network structure. Heating disrupts the weakest component in the system, namely the PNA−DNA hybrid, resulting in dissolution of the microgel, while cooling restores the hydrogen bonding leading to reassembly of the microgel. Variation of the nucleotide sequence permits tuning of the gelation temperature with fine control.</description><identifier>ISSN: 0002-7863</identifier><identifier>EISSN: 1520-5126</identifier><identifier>DOI: 10.1021/ja035211t</identifier><identifier>PMID: 12926948</identifier><identifier>CODEN: JACSAT</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Analytical, structural and metabolic biochemistry ; Avidin - chemistry ; Base Sequence ; Biological and medical sciences ; Biotin - chemistry ; DNA - chemical synthesis ; DNA - chemistry ; Fundamental and applied biological sciences. Psychology ; Gels ; General aspects, investigation methods ; Hot Temperature ; Hydrogen Bonding ; Light ; Molecular Sequence Data ; Nucleic Acid Conformation ; Nucleic Acid Hybridization ; Nucleic acids ; Oligonucleotides - chemical synthesis ; Oligonucleotides - chemistry ; Peptide Nucleic Acids - chemical synthesis ; Peptide Nucleic Acids - chemistry ; Proteins - chemical synthesis ; Proteins - chemistry ; Scattering, Radiation</subject><ispartof>Journal of the American Chemical Society, 2003-08, Vol.125 (34), p.10250-10256</ispartof><rights>Copyright © 2003 American Chemical Society</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a476t-72400790a33d59da703ec3a3be592e6b9e4a62e6537dd98c328d2088710c93cc3</citedby><cites>FETCH-LOGICAL-a476t-72400790a33d59da703ec3a3be592e6b9e4a62e6537dd98c328d2088710c93cc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ja035211t$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ja035211t$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15080245$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12926948$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cao, Rong</creatorcontrib><creatorcontrib>Gu, Zhenyu</creatorcontrib><creatorcontrib>Hsu, Lorraine</creatorcontrib><creatorcontrib>Patterson, Gary D</creatorcontrib><creatorcontrib>Armitage, Bruce A</creatorcontrib><title>Synthesis and Characterization of Thermoreversible Biopolymer Microgels Based on Hydrogen Bonded Nucleobase Pairing</title><title>Journal of the American Chemical Society</title><addtitle>J. Am. Chem. Soc</addtitle><description>We describe the synthesis and characterization of a thermoreversibly cross-linked biopolymer microgel based on protein, DNA, and peptide nucleic acid (PNA) components. The DNA assembles into a trifunctional three-way junction (TWJ) with single-stranded overhangs. PNA oligomers complementary to these overhangs and bearing terminal biotin groups hybridize to the DNA TWJ and simultaneously bind to the tetrafunctional protein avidin, leading to a cross-linked system. Dynamic light scattering experiments reveal that micron-sized particles are formed. Static light scattering was used to characterize the internal structure of these microgels, which were found to have a fractal dimension of 1.85, indicative of a loose network structure. Heating disrupts the weakest component in the system, namely the PNA−DNA hybrid, resulting in dissolution of the microgel, while cooling restores the hydrogen bonding leading to reassembly of the microgel. Variation of the nucleotide sequence permits tuning of the gelation temperature with fine control.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Avidin - chemistry</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biotin - chemistry</subject><subject>DNA - chemical synthesis</subject><subject>DNA - chemistry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gels</subject><subject>General aspects, investigation methods</subject><subject>Hot Temperature</subject><subject>Hydrogen Bonding</subject><subject>Light</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Conformation</subject><subject>Nucleic Acid Hybridization</subject><subject>Nucleic acids</subject><subject>Oligonucleotides - chemical synthesis</subject><subject>Oligonucleotides - chemistry</subject><subject>Peptide Nucleic Acids - chemical synthesis</subject><subject>Peptide Nucleic Acids - chemistry</subject><subject>Proteins - chemical synthesis</subject><subject>Proteins - chemistry</subject><subject>Scattering, Radiation</subject><issn>0002-7863</issn><issn>1520-5126</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1vEzEQhi0EoqFw4A8gX0DisOCP9dp7JBFQUAuVGi5crFl70jhs1sHeRYRfj6tEzQWJ03w988p-h5DnnL3hTPC3G2BSCc7HB2TGlWCV4qJ5SGaMMVFp08gz8iTnTSlrYfhjcsZFK5q2NjOSb_bDuMYcMoXB08UaErgRU_gDY4gDjSu6XGPaxoS_MOXQ9UjnIe5iv99iolfBpXiLfaZzyOhp2bjY-7vWQOdx8KX1ZXI9xq6M6TWEFIbbp-TRCvqMz47xnHz78H65uKguv378tHh3WUGtm7HSomZMtwyk9Kr1oJlEJ0F2qFqBTddiDU1JlNTet8ZJYbxgxmjOXCudk-fk1UF3l-LPCfNotyE77HsYME7ZaqmMblT9X5BrY0zxrICvD2D5dc4JV3aXwhbS3nJm705h709R2BdH0anboj-RR-8L8PIIQHbQrxIMLuQTp5hholaFqw5cyCP-vp9D-mEbLbWyy-sb26qruVh8_m6XJ11w2W7ilIZi8j8e-BfCIKyR</recordid><startdate>20030827</startdate><enddate>20030827</enddate><creator>Cao, Rong</creator><creator>Gu, Zhenyu</creator><creator>Hsu, Lorraine</creator><creator>Patterson, Gary D</creator><creator>Armitage, Bruce A</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>20030827</creationdate><title>Synthesis and Characterization of Thermoreversible Biopolymer Microgels Based on Hydrogen Bonded Nucleobase Pairing</title><author>Cao, Rong ; Gu, Zhenyu ; Hsu, Lorraine ; Patterson, Gary D ; Armitage, Bruce A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a476t-72400790a33d59da703ec3a3be592e6b9e4a62e6537dd98c328d2088710c93cc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Avidin - chemistry</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biotin - chemistry</topic><topic>DNA - chemical synthesis</topic><topic>DNA - chemistry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gels</topic><topic>General aspects, investigation methods</topic><topic>Hot Temperature</topic><topic>Hydrogen Bonding</topic><topic>Light</topic><topic>Molecular Sequence Data</topic><topic>Nucleic Acid Conformation</topic><topic>Nucleic Acid Hybridization</topic><topic>Nucleic acids</topic><topic>Oligonucleotides - chemical synthesis</topic><topic>Oligonucleotides - chemistry</topic><topic>Peptide Nucleic Acids - chemical synthesis</topic><topic>Peptide Nucleic Acids - chemistry</topic><topic>Proteins - chemical synthesis</topic><topic>Proteins - chemistry</topic><topic>Scattering, Radiation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cao, Rong</creatorcontrib><creatorcontrib>Gu, Zhenyu</creatorcontrib><creatorcontrib>Hsu, Lorraine</creatorcontrib><creatorcontrib>Patterson, Gary D</creatorcontrib><creatorcontrib>Armitage, Bruce A</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of the American Chemical Society</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cao, Rong</au><au>Gu, Zhenyu</au><au>Hsu, Lorraine</au><au>Patterson, Gary D</au><au>Armitage, Bruce A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synthesis and Characterization of Thermoreversible Biopolymer Microgels Based on Hydrogen Bonded Nucleobase Pairing</atitle><jtitle>Journal of the American Chemical Society</jtitle><addtitle>J. Am. Chem. Soc</addtitle><date>2003-08-27</date><risdate>2003</risdate><volume>125</volume><issue>34</issue><spage>10250</spage><epage>10256</epage><pages>10250-10256</pages><issn>0002-7863</issn><eissn>1520-5126</eissn><coden>JACSAT</coden><abstract>We describe the synthesis and characterization of a thermoreversibly cross-linked biopolymer microgel based on protein, DNA, and peptide nucleic acid (PNA) components. The DNA assembles into a trifunctional three-way junction (TWJ) with single-stranded overhangs. PNA oligomers complementary to these overhangs and bearing terminal biotin groups hybridize to the DNA TWJ and simultaneously bind to the tetrafunctional protein avidin, leading to a cross-linked system. Dynamic light scattering experiments reveal that micron-sized particles are formed. Static light scattering was used to characterize the internal structure of these microgels, which were found to have a fractal dimension of 1.85, indicative of a loose network structure. Heating disrupts the weakest component in the system, namely the PNA−DNA hybrid, resulting in dissolution of the microgel, while cooling restores the hydrogen bonding leading to reassembly of the microgel. Variation of the nucleotide sequence permits tuning of the gelation temperature with fine control.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>12926948</pmid><doi>10.1021/ja035211t</doi><tpages>7</tpages></addata></record> |
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| subjects | Analytical, structural and metabolic biochemistry Avidin - chemistry Base Sequence Biological and medical sciences Biotin - chemistry DNA - chemical synthesis DNA - chemistry Fundamental and applied biological sciences. Psychology Gels General aspects, investigation methods Hot Temperature Hydrogen Bonding Light Molecular Sequence Data Nucleic Acid Conformation Nucleic Acid Hybridization Nucleic acids Oligonucleotides - chemical synthesis Oligonucleotides - chemistry Peptide Nucleic Acids - chemical synthesis Peptide Nucleic Acids - chemistry Proteins - chemical synthesis Proteins - chemistry Scattering, Radiation |
| title | Synthesis and Characterization of Thermoreversible Biopolymer Microgels Based on Hydrogen Bonded Nucleobase Pairing |
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