Nerve trophic effects: Partial purification from chick embryo brains of proteins that stimulate protein synthesis in cultured newt blastemata

Eighteen-day embryonic chick brain homogenates stimulate the incorporation of 14C-amino acids into proteins of cultured early to medium bud stage newt blastemata. DEAE-cellulose column chromatography of the homogenate yields a fraction which is highly active but unstable. Electrophoresis of the homo...

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Veröffentlicht in:	Experimental neurology 1981-01, Vol.73 (2), p.558-570
Hauptverfasser:	Choo, Alfred F., Logan, David M., Rathbone, Michel P.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Brain Chemistry Cells, Cultured Chick Embryo chick embryos Chromatography, Ion Exchange Electrophoresis, Polyacrylamide Gel isolation Molecular Weight Nerve Growth Factors Nerve Tissue Proteins - biosynthesis Nerve Tissue Proteins - isolation & purification Nerve Tissue Proteins - pharmacology neurotropin Regeneration - drug effects Salamandridae - physiology
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