Improved and simple micro assay for sulfated glycosaminoglycans quantification in biological extracts and its use in skin and muscle tissue studies

This article describes a simple and selective procedure used for direct measurement of sulfated glycosaminoglycans (GAGs) in biological samples and its application to the determination of GAGs during tissue regeneration and myogenic differentiation. We describe a modified procedure of previous GAG a...

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Veröffentlicht in:	Glycobiology (Oxford) 2003-09, Vol.13 (9), p.647-653
Hauptverfasser:	Barbosa, Isabelle, Garcia, Stéphanie, Barbier-Chassefière, Véronique, Caruelle, Jean-Pierre, Martelly, Isabelle, Papy-García, Dulce
Format:	Artikel
Sprache:	eng
Schlagworte:	9-dimethylmethylene blue Animals Calibration cell culture Cell Differentiation Cell Line chondroitin sulfate coefficient of variation DAPI diaminophenyl indole DMEM DMMB Dulbecco modified Eagle medium FBS fetal bovine serum GAG glycosaminoglycan glycosaminoglycans Glycosaminoglycans - analysis guanidine hydrochloride GuHCl heparan sulfate Heparitin Sulfate Male Methylene Blue - analogs & derivatives Mice Muscles - cytology Muscles - metabolism Rats Rats, Wistar Regeneration Reproducibility of Results Sensitivity and Specificity skeletal muscle skin Skin - metabolism Skin - pathology
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