Pasteurella multocida- and Pasteurella haemolytica-ghosts: new vaccine candidates
Pasteurella multocida is an important animal pathogen. Bacterial ghosts produced by the expression of phage PhiX174 lysis gene E are empty cells devoid of cytoplasmic and genomic material. Lysis of P. multocida 7A and P. haemolytica A1 carrying Pasteurella-specific lysis vectors (pSR2 and pSON2) occ...
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| Veröffentlicht in: | Vaccine 2003-09, Vol.21 (25-26), p.3988-3997 |
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| creator | Marchart, J. Dropmann, G. Lechleitner, S. Schlapp, T. Wanner, G. Szostak, M.P. Lubitz, W. |
| description | Pasteurella multocida is an important animal pathogen. Bacterial ghosts produced by the expression of phage PhiX174 lysis gene E are empty cells devoid of cytoplasmic and genomic material. Lysis of P. multocida 7A and P. haemolytica A1 carrying Pasteurella-specific lysis vectors (pSR2 and pSON2) occurred 140min after induction of gene E expression induced by temperature upshift. The E-mediated cell lysis and killing activity was the same in both Pasteurella species and no viable cells could be detected after lysis of P. multocida and P. haemolytica.
Pasteurella ghosts were used for immunization of rabbits and mice. Rabbits immunized subcutaneously with either P. multocida- or P. haemolytica-ghosts developed antibodies reacting with the immunizating strain, as well as with other Pasteurella strains. The number of proteins in whole cell protein extracts recognized by the sera constantly increased during the observation period of 51 days. In addition, dose-dependent protection against homologous challenge was observed in mice immunized with P. multocida-ghosts. Animals which received 1.15×108 ghosts and a challenge dose of up to 60cfu (LD90), showed 100% protection. According to these results, we suggest ghosts of P. multocida and P. haemolytica as new vaccine candidates. |
| doi_str_mv | 10.1016/S0264-410X(03)00383-9 |
| format | Article |
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Pasteurella ghosts were used for immunization of rabbits and mice. Rabbits immunized subcutaneously with either P. multocida- or P. haemolytica-ghosts developed antibodies reacting with the immunizating strain, as well as with other Pasteurella strains. The number of proteins in whole cell protein extracts recognized by the sera constantly increased during the observation period of 51 days. In addition, dose-dependent protection against homologous challenge was observed in mice immunized with P. multocida-ghosts. Animals which received 1.15×108 ghosts and a challenge dose of up to 60cfu (LD90), showed 100% protection. According to these results, we suggest ghosts of P. multocida and P. haemolytica as new vaccine candidates.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/S0264-410X(03)00383-9</identifier><identifier>PMID: 12922135</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Animals ; Antibodies ; Antigens ; Bacterial ghost ; Bacterial Vaccines - immunology ; Bacteriophage phi X 174 - genetics ; Blotting, Western ; Deoxyribonucleic acid ; DNA ; DNA, Bacterial - genetics ; DNA, Recombinant - genetics ; Dose-Response Relationship, Immunologic ; E coli ; Electrophoresis, Polyacrylamide Gel ; Expression vectors ; Gene expression ; Ghosts ; Homology ; Immunization ; Immunoglobulins ; Lysis ; Mannheimia haemolytica - immunology ; Mannheimia haemolytica - ultrastructure ; Mannheimia haemolytica - virology ; Mice ; Microscopy, Electron, Scanning ; Pasteurella ; Pasteurella multocida ; Pasteurella multocida - immunology ; Pasteurella multocida - ultrastructure ; Pasteurella multocida - virology ; Phages ; Plasmids ; Plasmids - genetics ; Protection ; Proteins ; Rabbits ; Salmonella ; Temperature ; Transformation, Bacterial ; Vaccine ; Vaccines</subject><ispartof>Vaccine, 2003-09, Vol.21 (25-26), p.3988-3997</ispartof><rights>2003 Elsevier Science Ltd</rights><rights>2003. Elsevier Science Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c455t-bf02877949b26d537e0fa05fdde1c03dc1812242f2a67e8039414ca7f14a5fd93</citedby><cites>FETCH-LOGICAL-c455t-bf02877949b26d537e0fa05fdde1c03dc1812242f2a67e8039414ca7f14a5fd93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/2198005623?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995,64385,64387,64389,72469</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12922135$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Marchart, J.</creatorcontrib><creatorcontrib>Dropmann, G.</creatorcontrib><creatorcontrib>Lechleitner, S.</creatorcontrib><creatorcontrib>Schlapp, T.</creatorcontrib><creatorcontrib>Wanner, G.</creatorcontrib><creatorcontrib>Szostak, M.P.</creatorcontrib><creatorcontrib>Lubitz, W.</creatorcontrib><title>Pasteurella multocida- and Pasteurella haemolytica-ghosts: new vaccine candidates</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>Pasteurella multocida is an important animal pathogen. Bacterial ghosts produced by the expression of phage PhiX174 lysis gene E are empty cells devoid of cytoplasmic and genomic material. Lysis of P. multocida 7A and P. haemolytica A1 carrying Pasteurella-specific lysis vectors (pSR2 and pSON2) occurred 140min after induction of gene E expression induced by temperature upshift. The E-mediated cell lysis and killing activity was the same in both Pasteurella species and no viable cells could be detected after lysis of P. multocida and P. haemolytica.
Pasteurella ghosts were used for immunization of rabbits and mice. Rabbits immunized subcutaneously with either P. multocida- or P. haemolytica-ghosts developed antibodies reacting with the immunizating strain, as well as with other Pasteurella strains. The number of proteins in whole cell protein extracts recognized by the sera constantly increased during the observation period of 51 days. In addition, dose-dependent protection against homologous challenge was observed in mice immunized with P. multocida-ghosts. Animals which received 1.15×108 ghosts and a challenge dose of up to 60cfu (LD90), showed 100% protection. According to these results, we suggest ghosts of P. multocida and P. haemolytica as new vaccine candidates.</description><subject>Animals</subject><subject>Antibodies</subject><subject>Antigens</subject><subject>Bacterial ghost</subject><subject>Bacterial Vaccines - immunology</subject><subject>Bacteriophage phi X 174 - genetics</subject><subject>Blotting, Western</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Recombinant - genetics</subject><subject>Dose-Response Relationship, Immunologic</subject><subject>E coli</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Expression vectors</subject><subject>Gene expression</subject><subject>Ghosts</subject><subject>Homology</subject><subject>Immunization</subject><subject>Immunoglobulins</subject><subject>Lysis</subject><subject>Mannheimia haemolytica - immunology</subject><subject>Mannheimia haemolytica - ultrastructure</subject><subject>Mannheimia haemolytica - virology</subject><subject>Mice</subject><subject>Microscopy, Electron, Scanning</subject><subject>Pasteurella</subject><subject>Pasteurella multocida</subject><subject>Pasteurella multocida - immunology</subject><subject>Pasteurella multocida - ultrastructure</subject><subject>Pasteurella multocida - virology</subject><subject>Phages</subject><subject>Plasmids</subject><subject>Plasmids - genetics</subject><subject>Protection</subject><subject>Proteins</subject><subject>Rabbits</subject><subject>Salmonella</subject><subject>Temperature</subject><subject>Transformation, Bacterial</subject><subject>Vaccine</subject><subject>Vaccines</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqNkV1rFTEQhoMo9lj9CcqCUOrF1pl8bLLelFL8goKKCt6FnGTWpuxHTXZb-u_N6TmoeFG9ysU870xmHsaeIhwhYPPyM_BG1hLh2yGIFwDCiLq9x1ZotKi5QnOfrX4he-xRzhcAoAS2D9ke8pZzFGrFPn10eaYlUd-7alj6efIxuLpyY6j-LJ07Gqb-Zo7e1d_PpzznV9VI19WV8z6OVPkSKMGZ8mP2oHN9pie7d599ffP6y-m7-uzD2_enJ2e1l0rN9boDbrRuZbvmTVBCE3QOVBcCoQcRPBrkXPKOu0aTAdFKlN7pDqUrVCv22cG272WafiyUZzvE7DefHWlastVCGTANL-DhnSCqBlAgcvUfKIDW2ghZ0Od_oRfTksayseXYmnLqMrpQakv5NOWcqLOXKQ4u3VgEu_Fobz3ajSQLwt56tJvlnu26L-uBwu_UTlwBjrcAlRNfRUo2-0ijpxAT-dmGKf5jxE_6Jato</recordid><startdate>20030908</startdate><enddate>20030908</enddate><creator>Marchart, J.</creator><creator>Dropmann, G.</creator><creator>Lechleitner, S.</creator><creator>Schlapp, T.</creator><creator>Wanner, G.</creator><creator>Szostak, M.P.</creator><creator>Lubitz, W.</creator><general>Elsevier Ltd</general><general>Elsevier Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7RV</scope><scope>7T2</scope><scope>7T5</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88C</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0R</scope><scope>M0S</scope><scope>M0T</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20030908</creationdate><title>Pasteurella multocida- and Pasteurella haemolytica-ghosts: new vaccine candidates</title><author>Marchart, J. ; Dropmann, G. ; Lechleitner, S. ; Schlapp, T. ; Wanner, G. ; Szostak, M.P. ; Lubitz, W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c455t-bf02877949b26d537e0fa05fdde1c03dc1812242f2a67e8039414ca7f14a5fd93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Antibodies</topic><topic>Antigens</topic><topic>Bacterial ghost</topic><topic>Bacterial Vaccines - 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Academic</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Marchart, J.</au><au>Dropmann, G.</au><au>Lechleitner, S.</au><au>Schlapp, T.</au><au>Wanner, G.</au><au>Szostak, M.P.</au><au>Lubitz, W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pasteurella multocida- and Pasteurella haemolytica-ghosts: new vaccine candidates</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2003-09-08</date><risdate>2003</risdate><volume>21</volume><issue>25-26</issue><spage>3988</spage><epage>3997</epage><pages>3988-3997</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><abstract>Pasteurella multocida is an important animal pathogen. Bacterial ghosts produced by the expression of phage PhiX174 lysis gene E are empty cells devoid of cytoplasmic and genomic material. Lysis of P. multocida 7A and P. haemolytica A1 carrying Pasteurella-specific lysis vectors (pSR2 and pSON2) occurred 140min after induction of gene E expression induced by temperature upshift. The E-mediated cell lysis and killing activity was the same in both Pasteurella species and no viable cells could be detected after lysis of P. multocida and P. haemolytica.
Pasteurella ghosts were used for immunization of rabbits and mice. Rabbits immunized subcutaneously with either P. multocida- or P. haemolytica-ghosts developed antibodies reacting with the immunizating strain, as well as with other Pasteurella strains. The number of proteins in whole cell protein extracts recognized by the sera constantly increased during the observation period of 51 days. In addition, dose-dependent protection against homologous challenge was observed in mice immunized with P. multocida-ghosts. Animals which received 1.15×108 ghosts and a challenge dose of up to 60cfu (LD90), showed 100% protection. According to these results, we suggest ghosts of P. multocida and P. haemolytica as new vaccine candidates.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>12922135</pmid><doi>10.1016/S0264-410X(03)00383-9</doi><tpages>10</tpages></addata></record> |
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| subjects | Animals Antibodies Antigens Bacterial ghost Bacterial Vaccines - immunology Bacteriophage phi X 174 - genetics Blotting, Western Deoxyribonucleic acid DNA DNA, Bacterial - genetics DNA, Recombinant - genetics Dose-Response Relationship, Immunologic E coli Electrophoresis, Polyacrylamide Gel Expression vectors Gene expression Ghosts Homology Immunization Immunoglobulins Lysis Mannheimia haemolytica - immunology Mannheimia haemolytica - ultrastructure Mannheimia haemolytica - virology Mice Microscopy, Electron, Scanning Pasteurella Pasteurella multocida Pasteurella multocida - immunology Pasteurella multocida - ultrastructure Pasteurella multocida - virology Phages Plasmids Plasmids - genetics Protection Proteins Rabbits Salmonella Temperature Transformation, Bacterial Vaccine Vaccines |
| title | Pasteurella multocida- and Pasteurella haemolytica-ghosts: new vaccine candidates |
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