BMP-2 gene modified tissue-engineered bone repairing segmental tibial bone defects in goats
To evaluate the effectiveness of the tissue-engineered bone substitute loaded with adenovirus mediated human bone morphogenetic protein-2 gene (Adv-hBMP-2) transfected bone marrow derived mesenchymal stem cells (BMSC) in the repair of diaphyseal segmental bone defect of large animal. The right tibia...
Gespeichert in:
| Veröffentlicht in: | Zhong hua yi xue za zhi 2003-08, Vol.83 (15), p.1345-1349 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | chi |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1349 |
|---|---|
| container_issue | 15 |
| container_start_page | 1345 |
| container_title | Zhong hua yi xue za zhi |
| container_volume | 83 |
| creator | Dai, Ke-rong Xu, Xiao-liang Tang, Ting-ting Zhu, Zhen-an Yu, Chao-feng Xu, Min Zhu, Liu-long Hao, Yong-qiang Lou, Jue-ren |
| description | To evaluate the effectiveness of the tissue-engineered bone substitute loaded with adenovirus mediated human bone morphogenetic protein-2 gene (Adv-hBMP-2) transfected bone marrow derived mesenchymal stem cells (BMSC) in the repair of diaphyseal segmental bone defect of large animal.
The right tibial bone defects (2.6 cm) model of 26 goats were established and divided into 5 groups: I. Adv-hBMP-2 transfected BMSC/calcined bone (CB) group (n = 9); II. adenovirus-beta-galactosidase (Adv-betagal) gene transfected BMSC/CB group (n = 6); III. untransfected BMSC/CB group (n = 6); IV. single CB group (n = 3); VI. untreated group (n = 2). The above tissue-engineered bone substitutes were implanted in the bone defects respectively except group VI. Roentgenography, histomorphometrical analysis and biomechanical measurement were studied at various times.
X-ray: at 4 - 8th weeks after implantation, more bony callus was found in the bone defects of group I. The complete healing rates of group I, II, III, IV, and V were 5/ |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_73578983</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>73578983</sourcerecordid><originalsourceid>FETCH-LOGICAL-p122t-adc7c43dd78aafe669ebfd62db9a540c6dd7d1e748c66770e43e332aabd02e103</originalsourceid><addsrcrecordid>eNo1kLtOxDAURF2A2BXsLyBXdJH8WjsuYcVLWgQFVBSRHzeRpcQJtlPw91iwVCPNOZpiztCWcCUbJjTdoF3OwRKhuGaE0Qu0oUxzIjXbos-7l7eG4QEi4Gn2oQ_gcQk5r9BAHEIESLWxc-UJFhNSiAPOMEwQixmrakONX-6hB1cyDhEPsyn5Cp33ZsywO-Ul-ni4fz88NcfXx-fD7bFZKGOlMd4pJ7j3qjWmByk12N5L5q02e0GcrMRTUKJ1UipFQHDgnBljPWFACb9EN3-7S5q_Vsilm0J2MI4mwrzmTvG9anXLq3h9Elc7ge-WFCaTvrv_O_gPQlJc8w</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>73578983</pqid></control><display><type>article</type><title>BMP-2 gene modified tissue-engineered bone repairing segmental tibial bone defects in goats</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Dai, Ke-rong ; Xu, Xiao-liang ; Tang, Ting-ting ; Zhu, Zhen-an ; Yu, Chao-feng ; Xu, Min ; Zhu, Liu-long ; Hao, Yong-qiang ; Lou, Jue-ren</creator><creatorcontrib>Dai, Ke-rong ; Xu, Xiao-liang ; Tang, Ting-ting ; Zhu, Zhen-an ; Yu, Chao-feng ; Xu, Min ; Zhu, Liu-long ; Hao, Yong-qiang ; Lou, Jue-ren</creatorcontrib><description>To evaluate the effectiveness of the tissue-engineered bone substitute loaded with adenovirus mediated human bone morphogenetic protein-2 gene (Adv-hBMP-2) transfected bone marrow derived mesenchymal stem cells (BMSC) in the repair of diaphyseal segmental bone defect of large animal.
The right tibial bone defects (2.6 cm) model of 26 goats were established and divided into 5 groups: I. Adv-hBMP-2 transfected BMSC/calcined bone (CB) group (n = 9); II. adenovirus-beta-galactosidase (Adv-betagal) gene transfected BMSC/CB group (n = 6); III. untransfected BMSC/CB group (n = 6); IV. single CB group (n = 3); VI. untreated group (n = 2). The above tissue-engineered bone substitutes were implanted in the bone defects respectively except group VI. Roentgenography, histomorphometrical analysis and biomechanical measurement were studied at various times.
X-ray: at 4 - 8th weeks after implantation, more bony callus was found in the bone defects of group I. The complete healing rates of group I, II, III, IV, and V were 5/</description><identifier>ISSN: 0376-2491</identifier><identifier>PMID: 12930692</identifier><language>chi</language><publisher>China</publisher><subject>Animals ; Biomechanical Phenomena ; Bone Diseases - therapy ; Bone Morphogenetic Protein 2 ; Bone Morphogenetic Proteins - genetics ; Genetic Therapy ; Goats ; Mesenchymal Stem Cell Transplantation ; Tibia ; Tissue Engineering ; Transforming Growth Factor beta ; Wound Healing</subject><ispartof>Zhong hua yi xue za zhi, 2003-08, Vol.83 (15), p.1345-1349</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12930692$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dai, Ke-rong</creatorcontrib><creatorcontrib>Xu, Xiao-liang</creatorcontrib><creatorcontrib>Tang, Ting-ting</creatorcontrib><creatorcontrib>Zhu, Zhen-an</creatorcontrib><creatorcontrib>Yu, Chao-feng</creatorcontrib><creatorcontrib>Xu, Min</creatorcontrib><creatorcontrib>Zhu, Liu-long</creatorcontrib><creatorcontrib>Hao, Yong-qiang</creatorcontrib><creatorcontrib>Lou, Jue-ren</creatorcontrib><title>BMP-2 gene modified tissue-engineered bone repairing segmental tibial bone defects in goats</title><title>Zhong hua yi xue za zhi</title><addtitle>Zhonghua Yi Xue Za Zhi</addtitle><description>To evaluate the effectiveness of the tissue-engineered bone substitute loaded with adenovirus mediated human bone morphogenetic protein-2 gene (Adv-hBMP-2) transfected bone marrow derived mesenchymal stem cells (BMSC) in the repair of diaphyseal segmental bone defect of large animal.
The right tibial bone defects (2.6 cm) model of 26 goats were established and divided into 5 groups: I. Adv-hBMP-2 transfected BMSC/calcined bone (CB) group (n = 9); II. adenovirus-beta-galactosidase (Adv-betagal) gene transfected BMSC/CB group (n = 6); III. untransfected BMSC/CB group (n = 6); IV. single CB group (n = 3); VI. untreated group (n = 2). The above tissue-engineered bone substitutes were implanted in the bone defects respectively except group VI. Roentgenography, histomorphometrical analysis and biomechanical measurement were studied at various times.
X-ray: at 4 - 8th weeks after implantation, more bony callus was found in the bone defects of group I. The complete healing rates of group I, II, III, IV, and V were 5/</description><subject>Animals</subject><subject>Biomechanical Phenomena</subject><subject>Bone Diseases - therapy</subject><subject>Bone Morphogenetic Protein 2</subject><subject>Bone Morphogenetic Proteins - genetics</subject><subject>Genetic Therapy</subject><subject>Goats</subject><subject>Mesenchymal Stem Cell Transplantation</subject><subject>Tibia</subject><subject>Tissue Engineering</subject><subject>Transforming Growth Factor beta</subject><subject>Wound Healing</subject><issn>0376-2491</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kLtOxDAURF2A2BXsLyBXdJH8WjsuYcVLWgQFVBSRHzeRpcQJtlPw91iwVCPNOZpiztCWcCUbJjTdoF3OwRKhuGaE0Qu0oUxzIjXbos-7l7eG4QEi4Gn2oQ_gcQk5r9BAHEIESLWxc-UJFhNSiAPOMEwQixmrakONX-6hB1cyDhEPsyn5Cp33ZsywO-Ul-ni4fz88NcfXx-fD7bFZKGOlMd4pJ7j3qjWmByk12N5L5q02e0GcrMRTUKJ1UipFQHDgnBljPWFACb9EN3-7S5q_Vsilm0J2MI4mwrzmTvG9anXLq3h9Elc7ge-WFCaTvrv_O_gPQlJc8w</recordid><startdate>20030810</startdate><enddate>20030810</enddate><creator>Dai, Ke-rong</creator><creator>Xu, Xiao-liang</creator><creator>Tang, Ting-ting</creator><creator>Zhu, Zhen-an</creator><creator>Yu, Chao-feng</creator><creator>Xu, Min</creator><creator>Zhu, Liu-long</creator><creator>Hao, Yong-qiang</creator><creator>Lou, Jue-ren</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20030810</creationdate><title>BMP-2 gene modified tissue-engineered bone repairing segmental tibial bone defects in goats</title><author>Dai, Ke-rong ; Xu, Xiao-liang ; Tang, Ting-ting ; Zhu, Zhen-an ; Yu, Chao-feng ; Xu, Min ; Zhu, Liu-long ; Hao, Yong-qiang ; Lou, Jue-ren</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p122t-adc7c43dd78aafe669ebfd62db9a540c6dd7d1e748c66770e43e332aabd02e103</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Biomechanical Phenomena</topic><topic>Bone Diseases - therapy</topic><topic>Bone Morphogenetic Protein 2</topic><topic>Bone Morphogenetic Proteins - genetics</topic><topic>Genetic Therapy</topic><topic>Goats</topic><topic>Mesenchymal Stem Cell Transplantation</topic><topic>Tibia</topic><topic>Tissue Engineering</topic><topic>Transforming Growth Factor beta</topic><topic>Wound Healing</topic><toplevel>online_resources</toplevel><creatorcontrib>Dai, Ke-rong</creatorcontrib><creatorcontrib>Xu, Xiao-liang</creatorcontrib><creatorcontrib>Tang, Ting-ting</creatorcontrib><creatorcontrib>Zhu, Zhen-an</creatorcontrib><creatorcontrib>Yu, Chao-feng</creatorcontrib><creatorcontrib>Xu, Min</creatorcontrib><creatorcontrib>Zhu, Liu-long</creatorcontrib><creatorcontrib>Hao, Yong-qiang</creatorcontrib><creatorcontrib>Lou, Jue-ren</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Zhong hua yi xue za zhi</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dai, Ke-rong</au><au>Xu, Xiao-liang</au><au>Tang, Ting-ting</au><au>Zhu, Zhen-an</au><au>Yu, Chao-feng</au><au>Xu, Min</au><au>Zhu, Liu-long</au><au>Hao, Yong-qiang</au><au>Lou, Jue-ren</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>BMP-2 gene modified tissue-engineered bone repairing segmental tibial bone defects in goats</atitle><jtitle>Zhong hua yi xue za zhi</jtitle><addtitle>Zhonghua Yi Xue Za Zhi</addtitle><date>2003-08-10</date><risdate>2003</risdate><volume>83</volume><issue>15</issue><spage>1345</spage><epage>1349</epage><pages>1345-1349</pages><issn>0376-2491</issn><abstract>To evaluate the effectiveness of the tissue-engineered bone substitute loaded with adenovirus mediated human bone morphogenetic protein-2 gene (Adv-hBMP-2) transfected bone marrow derived mesenchymal stem cells (BMSC) in the repair of diaphyseal segmental bone defect of large animal.
The right tibial bone defects (2.6 cm) model of 26 goats were established and divided into 5 groups: I. Adv-hBMP-2 transfected BMSC/calcined bone (CB) group (n = 9); II. adenovirus-beta-galactosidase (Adv-betagal) gene transfected BMSC/CB group (n = 6); III. untransfected BMSC/CB group (n = 6); IV. single CB group (n = 3); VI. untreated group (n = 2). The above tissue-engineered bone substitutes were implanted in the bone defects respectively except group VI. Roentgenography, histomorphometrical analysis and biomechanical measurement were studied at various times.
X-ray: at 4 - 8th weeks after implantation, more bony callus was found in the bone defects of group I. The complete healing rates of group I, II, III, IV, and V were 5/</abstract><cop>China</cop><pmid>12930692</pmid><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0376-2491 |
| ispartof | Zhong hua yi xue za zhi, 2003-08, Vol.83 (15), p.1345-1349 |
| issn | 0376-2491 |
| language | chi |
| recordid | cdi_proquest_miscellaneous_73578983 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Biomechanical Phenomena Bone Diseases - therapy Bone Morphogenetic Protein 2 Bone Morphogenetic Proteins - genetics Genetic Therapy Goats Mesenchymal Stem Cell Transplantation Tibia Tissue Engineering Transforming Growth Factor beta Wound Healing |
| title | BMP-2 gene modified tissue-engineered bone repairing segmental tibial bone defects in goats |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-01T17%3A24%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=BMP-2%20gene%20modified%20tissue-engineered%20bone%20repairing%20segmental%20tibial%20bone%20defects%20in%20goats&rft.jtitle=Zhong%20hua%20yi%20xue%20za%20zhi&rft.au=Dai,%20Ke-rong&rft.date=2003-08-10&rft.volume=83&rft.issue=15&rft.spage=1345&rft.epage=1349&rft.pages=1345-1349&rft.issn=0376-2491&rft_id=info:doi/&rft_dat=%3Cproquest_pubme%3E73578983%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=73578983&rft_id=info:pmid/12930692&rfr_iscdi=true |