An ELISA for the detection of TIMP-1 based on recombinant single chain Fv fusion proteins

Background: Altered serum levels of TIMP-1 are an indicator of various pathological states. To quantitate TIMP-1 in biological samples, we have previously isolated TIMP-1 specific single-chain Fv fragments (scFvs) using phage-display. In the work presented here, we have used these scFvs to establish...

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Veröffentlicht in:	Clinica chimica acta 2003-09, Vol.335 (1), p.49-57
Hauptverfasser:	Wozniak, Gordana, Obermayr, Eva, Jeras, Matjaz, Knezevic, Mio, Rüker, Florian
Format:	Artikel
Sprache:	eng
Schlagworte:	Alkaline phosphatase Biological and medical sciences Cloning, Molecular ELISA Enzyme-Linked Immunosorbent Assay - methods Escherichia coli Fusion protein Humans Immunoglobulin Fragments - immunology Immunoglobulin Variable Region - immunology Investigative techniques, diagnostic techniques (general aspects) Medical sciences Miscellaneous. Technology Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Phage display Recombinant Fusion Proteins - biosynthesis Single-chain Fv TIMP-1 Tissue Inhibitor of Metalloproteinase-1 - analysis Tissue Inhibitor of Metalloproteinase-1 - blood Tissue Inhibitor of Metalloproteinase-1 - immunology
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