Short-term effect of epidermal growth factor on glucose uptake in endoscopic biopsies
Epidermal growth factor (EGF) up-regulation of glucose absorption via increased Na+/glucose co-transporter (SGLT-1) activity has previously been described in rabbit jejunal brush-border membrane and in differentiated Caco-2 cells. The goal of the present study was to assess the in vitro effect of EG...
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| Veröffentlicht in: | Digestive diseases and sciences 2003-08, Vol.48 (8), p.1614-1618 |
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| creator | Tonb, Dalal Mehta, Raj Wang, Hugh Tung, John Mehta, Devendra I |
| description | Epidermal growth factor (EGF) up-regulation of glucose absorption via increased Na+/glucose co-transporter (SGLT-1) activity has previously been described in rabbit jejunal brush-border membrane and in differentiated Caco-2 cells. The goal of the present study was to assess the in vitro effect of EGF (200 ng/ml) on glucose uptake in human mucosal specimens, and we describe a simple procedure that uses endoscopic biopsies for short-term gludose uptake measurements. Uptake values for the EGF-treated biopsies ranged from 2.7 to 29.0, with a mean uptake of 10.65, while uptake values for the untreated biopsies ranged from 0.9 to 17.5, with a mean uptake of 7.99 (P < 0.05, paired t test). This early effect of EGF on human enterocytes may have important therapeutic implications. A role in increasing the rate of internal rehydration is suggested. |
| doi_str_mv | 10.1023/A:1024780226875 |
| format | Article |
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The goal of the present study was to assess the in vitro effect of EGF (200 ng/ml) on glucose uptake in human mucosal specimens, and we describe a simple procedure that uses endoscopic biopsies for short-term gludose uptake measurements. Uptake values for the EGF-treated biopsies ranged from 2.7 to 29.0, with a mean uptake of 10.65, while uptake values for the untreated biopsies ranged from 0.9 to 17.5, with a mean uptake of 7.99 (P < 0.05, paired t test). This early effect of EGF on human enterocytes may have important therapeutic implications. A role in increasing the rate of internal rehydration is suggested.</description><identifier>ISSN: 0163-2116</identifier><identifier>EISSN: 1573-2568</identifier><identifier>DOI: 10.1023/A:1024780226875</identifier><identifier>PMID: 12924656</identifier><identifier>CODEN: DDSCDJ</identifier><language>eng</language><publisher>United States: Springer Nature B.V</publisher><subject>Adolescent ; Biopsy ; Child ; Child, Preschool ; Culture Techniques ; Endoscopy, Gastrointestinal ; Enterocytes - drug effects ; Epidermal Growth Factor - pharmacology ; Female ; Glucose - metabolism ; Humans ; Infant ; Intestinal Absorption - drug effects ; Intestinal Mucosa - drug effects ; Male ; Membrane Glycoproteins - physiology ; Methylglucosides - metabolism ; Monosaccharide Transport Proteins - physiology ; Sodium-Glucose Transporter 1 ; Water-Electrolyte Balance - drug effects</subject><ispartof>Digestive diseases and sciences, 2003-08, Vol.48 (8), p.1614-1618</ispartof><rights>Copyright Kluwer Academic Publishers Aug 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12924656$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tonb, Dalal</creatorcontrib><creatorcontrib>Mehta, Raj</creatorcontrib><creatorcontrib>Wang, Hugh</creatorcontrib><creatorcontrib>Tung, John</creatorcontrib><creatorcontrib>Mehta, Devendra I</creatorcontrib><title>Short-term effect of epidermal growth factor on glucose uptake in endoscopic biopsies</title><title>Digestive diseases and sciences</title><addtitle>Dig Dis Sci</addtitle><description>Epidermal growth factor (EGF) up-regulation of glucose absorption via increased Na+/glucose co-transporter (SGLT-1) activity has previously been described in rabbit jejunal brush-border membrane and in differentiated Caco-2 cells. 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| subjects | Adolescent Biopsy Child Child, Preschool Culture Techniques Endoscopy, Gastrointestinal Enterocytes - drug effects Epidermal Growth Factor - pharmacology Female Glucose - metabolism Humans Infant Intestinal Absorption - drug effects Intestinal Mucosa - drug effects Male Membrane Glycoproteins - physiology Methylglucosides - metabolism Monosaccharide Transport Proteins - physiology Sodium-Glucose Transporter 1 Water-Electrolyte Balance - drug effects |
| title | Short-term effect of epidermal growth factor on glucose uptake in endoscopic biopsies |
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