Protonation of deoxycytidine residues in dC4 tetraloops: UV spectrophotometric study of dC10 and d(A14C4T14)

It is shown that component analysis could be applied to study the UV difference spectra of cytidine oligomers and hairpin oligonucleotides with cytidines in the loop region in order to account for the melting and titration results in terms of cytidine stacking and protonation. Upon acid titration, t...

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Veröffentlicht in:	Biophysical chemistry 2003-06, Vol.104 (2), p.429-447
Hauptverfasser:	Raukas, E, Kooli, K
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Pairing Deoxycytidine - chemistry Hydrogen-Ion Concentration Nucleic Acid Denaturation Oligodeoxyribonucleotides - chemistry Protons Spectrophotometry, Ultraviolet Thermodynamics Titrimetry Transition Temperature
Online-Zugang:	Volltext
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