Temporal regulation of chondrocyte metabolism in agarose constructs subjected to dynamic compression
The temporal response of chondrocyte metabolism in agarose constructs subjected to different dynamic compression regimes was investigated. The current study explored the effects of continuous or intermittent compression using various duty cycles of dynamic compressive loading, over a 48 h culture pe...
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| Veröffentlicht in: | Archives of biochemistry and biophysics 2003-09, Vol.417 (1), p.105-111 |
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| container_title | Archives of biochemistry and biophysics |
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| creator | Chowdhury, Tina T Bader, Dan L Shelton, Julia C Lee, David A |
| description | The temporal response of chondrocyte metabolism in agarose constructs subjected to different dynamic compression regimes was investigated. The current study explored the effects of continuous or intermittent compression using various duty cycles of dynamic compressive loading, over a 48
h culture period. For the continuous compression experiments, duty cycles ranged from 5400 to 172,800 and intermittent compression delivered a total of 86,400 cycles. Large numbers of duty cycles significantly stimulated proteoglycan synthesis with maximal levels obtained for constructs subjected to 12
h of intermittent compression. The shortest duration of intermittent compression suggested that further cycles are inhibitory for cell proliferation. Nitrite release was independent of the length or type of compressive regime applied. The uncoupled nature of the metabolic response determined in this study suggests that mechanical conditioning regimes may be fine tuned to selectively stimulate key metabolic parameters of relevance to cartilage tissue engineering. |
| doi_str_mv | 10.1016/S0003-9861(03)00340-0 |
| format | Article |
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h culture period. For the continuous compression experiments, duty cycles ranged from 5400 to 172,800 and intermittent compression delivered a total of 86,400 cycles. Large numbers of duty cycles significantly stimulated proteoglycan synthesis with maximal levels obtained for constructs subjected to 12
h of intermittent compression. The shortest duration of intermittent compression suggested that further cycles are inhibitory for cell proliferation. Nitrite release was independent of the length or type of compressive regime applied. The uncoupled nature of the metabolic response determined in this study suggests that mechanical conditioning regimes may be fine tuned to selectively stimulate key metabolic parameters of relevance to cartilage tissue engineering.</description><identifier>ISSN: 0003-9861</identifier><identifier>EISSN: 1096-0384</identifier><identifier>DOI: 10.1016/S0003-9861(03)00340-0</identifier><identifier>PMID: 12921786</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Cartilage, Articular - cytology ; Cartilage, Articular - metabolism ; Cattle ; Cell Division ; Cells, Cultured ; Chondrocyte metabolism ; Chondrocytes - cytology ; Chondrocytes - metabolism ; Compressive Strength ; Dynamic compression ; Male ; Mechanical conditioning ; Nitrites - metabolism ; Proteoglycans - biosynthesis ; Sepharose ; Stress, Mechanical ; Sulfates - metabolism ; Sulfur Radioisotopes ; Thymidine - metabolism ; Time Factors</subject><ispartof>Archives of biochemistry and biophysics, 2003-09, Vol.417 (1), p.105-111</ispartof><rights>2003 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c427t-ddfca08cc4f509266326d735d778deb5c5e152ef2f7ea818c56c81e4ab55b3303</citedby><cites>FETCH-LOGICAL-c427t-ddfca08cc4f509266326d735d778deb5c5e152ef2f7ea818c56c81e4ab55b3303</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0003986103003400$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12921786$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chowdhury, Tina T</creatorcontrib><creatorcontrib>Bader, Dan L</creatorcontrib><creatorcontrib>Shelton, Julia C</creatorcontrib><creatorcontrib>Lee, David A</creatorcontrib><title>Temporal regulation of chondrocyte metabolism in agarose constructs subjected to dynamic compression</title><title>Archives of biochemistry and biophysics</title><addtitle>Arch Biochem Biophys</addtitle><description>The temporal response of chondrocyte metabolism in agarose constructs subjected to different dynamic compression regimes was investigated. The current study explored the effects of continuous or intermittent compression using various duty cycles of dynamic compressive loading, over a 48
h culture period. For the continuous compression experiments, duty cycles ranged from 5400 to 172,800 and intermittent compression delivered a total of 86,400 cycles. Large numbers of duty cycles significantly stimulated proteoglycan synthesis with maximal levels obtained for constructs subjected to 12
h of intermittent compression. The shortest duration of intermittent compression suggested that further cycles are inhibitory for cell proliferation. Nitrite release was independent of the length or type of compressive regime applied. The uncoupled nature of the metabolic response determined in this study suggests that mechanical conditioning regimes may be fine tuned to selectively stimulate key metabolic parameters of relevance to cartilage tissue engineering.</description><subject>Animals</subject><subject>Cartilage, Articular - cytology</subject><subject>Cartilage, Articular - metabolism</subject><subject>Cattle</subject><subject>Cell Division</subject><subject>Cells, Cultured</subject><subject>Chondrocyte metabolism</subject><subject>Chondrocytes - cytology</subject><subject>Chondrocytes - metabolism</subject><subject>Compressive Strength</subject><subject>Dynamic compression</subject><subject>Male</subject><subject>Mechanical conditioning</subject><subject>Nitrites - metabolism</subject><subject>Proteoglycans - biosynthesis</subject><subject>Sepharose</subject><subject>Stress, Mechanical</subject><subject>Sulfates - metabolism</subject><subject>Sulfur Radioisotopes</subject><subject>Thymidine - metabolism</subject><subject>Time Factors</subject><issn>0003-9861</issn><issn>1096-0384</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1P3DAQhq2qCBbKTyjyqSqHgD9iJzkhhNqChMQBerac8QSMknhrO0j77-tlV-XY01jyM--reQj5ytkFZ1xfPjLGZNW1mn9n8ry8a1axT2TFWacrJtv6M1n9Q47IcUqvjHFea3FIjrjoBG9avSLuCad1iHakEZ-X0WYfZhoGCi9hdjHAJiOdMNs-jD5N1M_UPtsYElIIc8pxgZxoWvpXhIyO5kDdZraTh_I_rSOmVAK_kIPBjglP9_OE_P754-nmtrp_-HV3c31fQS2aXDk3gGUtQD0o1gmtpdCukco1TeuwV6CQK4GDGBq0LW9BaWg51rZXqpeSyRPybZe7juHPgimbySfAcbQzhiWZkqW6RooCqh0I5ZQUcTDr6CcbN4Yzs9Vr3vWarTtT5rtesy042xcs_YTuY2vvswBXOwDLmW8eo0ngcQZ0PhZDxgX_n4q_Mk2Mag</recordid><startdate>20030901</startdate><enddate>20030901</enddate><creator>Chowdhury, Tina T</creator><creator>Bader, Dan L</creator><creator>Shelton, Julia C</creator><creator>Lee, David A</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20030901</creationdate><title>Temporal regulation of chondrocyte metabolism in agarose constructs subjected to dynamic compression</title><author>Chowdhury, Tina T ; Bader, Dan L ; Shelton, Julia C ; Lee, David A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c427t-ddfca08cc4f509266326d735d778deb5c5e152ef2f7ea818c56c81e4ab55b3303</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Cartilage, Articular - cytology</topic><topic>Cartilage, Articular - metabolism</topic><topic>Cattle</topic><topic>Cell Division</topic><topic>Cells, Cultured</topic><topic>Chondrocyte metabolism</topic><topic>Chondrocytes - cytology</topic><topic>Chondrocytes - metabolism</topic><topic>Compressive Strength</topic><topic>Dynamic compression</topic><topic>Male</topic><topic>Mechanical conditioning</topic><topic>Nitrites - metabolism</topic><topic>Proteoglycans - biosynthesis</topic><topic>Sepharose</topic><topic>Stress, Mechanical</topic><topic>Sulfates - metabolism</topic><topic>Sulfur Radioisotopes</topic><topic>Thymidine - metabolism</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chowdhury, Tina T</creatorcontrib><creatorcontrib>Bader, Dan L</creatorcontrib><creatorcontrib>Shelton, Julia C</creatorcontrib><creatorcontrib>Lee, David A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of biochemistry and biophysics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chowdhury, Tina T</au><au>Bader, Dan L</au><au>Shelton, Julia C</au><au>Lee, David A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Temporal regulation of chondrocyte metabolism in agarose constructs subjected to dynamic compression</atitle><jtitle>Archives of biochemistry and biophysics</jtitle><addtitle>Arch Biochem Biophys</addtitle><date>2003-09-01</date><risdate>2003</risdate><volume>417</volume><issue>1</issue><spage>105</spage><epage>111</epage><pages>105-111</pages><issn>0003-9861</issn><eissn>1096-0384</eissn><abstract>The temporal response of chondrocyte metabolism in agarose constructs subjected to different dynamic compression regimes was investigated. The current study explored the effects of continuous or intermittent compression using various duty cycles of dynamic compressive loading, over a 48
h culture period. For the continuous compression experiments, duty cycles ranged from 5400 to 172,800 and intermittent compression delivered a total of 86,400 cycles. Large numbers of duty cycles significantly stimulated proteoglycan synthesis with maximal levels obtained for constructs subjected to 12
h of intermittent compression. The shortest duration of intermittent compression suggested that further cycles are inhibitory for cell proliferation. Nitrite release was independent of the length or type of compressive regime applied. The uncoupled nature of the metabolic response determined in this study suggests that mechanical conditioning regimes may be fine tuned to selectively stimulate key metabolic parameters of relevance to cartilage tissue engineering.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>12921786</pmid><doi>10.1016/S0003-9861(03)00340-0</doi><tpages>7</tpages></addata></record> |
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| ispartof | Archives of biochemistry and biophysics, 2003-09, Vol.417 (1), p.105-111 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Cartilage, Articular - cytology Cartilage, Articular - metabolism Cattle Cell Division Cells, Cultured Chondrocyte metabolism Chondrocytes - cytology Chondrocytes - metabolism Compressive Strength Dynamic compression Male Mechanical conditioning Nitrites - metabolism Proteoglycans - biosynthesis Sepharose Stress, Mechanical Sulfates - metabolism Sulfur Radioisotopes Thymidine - metabolism Time Factors |
| title | Temporal regulation of chondrocyte metabolism in agarose constructs subjected to dynamic compression |
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