Technetium-99m scintigraphy to visualize T-cell homing in vivo: a preclinical study
The knowledge of lymphocyte distribution is very usefulness in monitoring therapeutic treatments. We present here a method employed in clinical practice, the scintigraphy, to study in the rat the physiologic lymphocyte traffic. Rat T cells labeled with 99mTc were injected in syngeneic animals, and t...
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| Veröffentlicht in: | Nuclear medicine and biology 2003-08, Vol.30 (6), p.633-642 |
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| creator | Fulgenzi, Alessandro Ferrero, Elisabetta Gasparini, Massimo Casati, Rosangela Riccardo Colombo, Fabio Gerundini, Paolo Elena Ferrero, Maria |
| description | The knowledge of lymphocyte distribution is very usefulness in monitoring therapeutic treatments. We present here a method employed in clinical practice, the scintigraphy, to study in the rat the physiologic lymphocyte traffic.
Rat T cells labeled with
99mTc were injected in syngeneic animals, and their fate was studied by serial scintigraphic scanning. Sorted naïve CD4
+ CD45RC
bright T cells homed to lymphoid organs and accumulated in spleen. CD4
+ CD45RC
dim memory lymphocytes first reached the liver and the lungs and recirculated.
The results obtained by using the scintigraphic method to in vivo study the lymphocyte homing in rats are comparable to those obtained with previously used experimental methods. We consider the scintigraphic method a useful tool to in vivo track lymphocytes and to address therapeutic treatment in men. |
| doi_str_mv | 10.1016/S0969-8051(03)00051-9 |
| format | Article |
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Rat T cells labeled with
99mTc were injected in syngeneic animals, and their fate was studied by serial scintigraphic scanning. Sorted naïve CD4
+ CD45RC
bright T cells homed to lymphoid organs and accumulated in spleen. CD4
+ CD45RC
dim memory lymphocytes first reached the liver and the lungs and recirculated.
The results obtained by using the scintigraphic method to in vivo study the lymphocyte homing in rats are comparable to those obtained with previously used experimental methods. We consider the scintigraphic method a useful tool to in vivo track lymphocytes and to address therapeutic treatment in men.</description><identifier>ISSN: 0969-8051</identifier><identifier>EISSN: 1872-9614</identifier><identifier>DOI: 10.1016/S0969-8051(03)00051-9</identifier><identifier>PMID: 12900289</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Animals ; Biological and medical sciences ; CD4 Lymphocyte Count - methods ; CD4-Positive T-Lymphocytes - diagnostic imaging ; CD4-Positive T-Lymphocytes - immunology ; CD4-Positive T-Lymphocytes - physiology ; Cell homing ; Cell Movement - immunology ; Cell Movement - physiology ; Lymphoid Tissue - diagnostic imaging ; Lymphoid Tissue - immunology ; Male ; Medical sciences ; Memory cells ; Naïve cells ; Organ Specificity ; Radionuclide Imaging ; Radiopharmaceuticals ; Rat ; Rats ; Rats, Inbred F344 ; Scintigraphy ; T lymphocytes ; T-Lymphocytes - diagnostic imaging ; T-Lymphocytes - immunology ; T-Lymphocytes - physiology ; Technetium Tc 99m Exametazime</subject><ispartof>Nuclear medicine and biology, 2003-08, Vol.30 (6), p.633-642</ispartof><rights>2003 Elsevier Inc.</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-f549d868614a18b79a3ecace312e932e1ea6711a3a4ea5ca294403340169fa223</citedby><cites>FETCH-LOGICAL-c391t-f549d868614a18b79a3ecace312e932e1ea6711a3a4ea5ca294403340169fa223</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0969-8051(03)00051-9$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15063928$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12900289$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fulgenzi, Alessandro</creatorcontrib><creatorcontrib>Ferrero, Elisabetta</creatorcontrib><creatorcontrib>Gasparini, Massimo</creatorcontrib><creatorcontrib>Casati, Rosangela</creatorcontrib><creatorcontrib>Riccardo Colombo, Fabio</creatorcontrib><creatorcontrib>Gerundini, Paolo</creatorcontrib><creatorcontrib>Elena Ferrero, Maria</creatorcontrib><title>Technetium-99m scintigraphy to visualize T-cell homing in vivo: a preclinical study</title><title>Nuclear medicine and biology</title><addtitle>Nucl Med Biol</addtitle><description>The knowledge of lymphocyte distribution is very usefulness in monitoring therapeutic treatments. We present here a method employed in clinical practice, the scintigraphy, to study in the rat the physiologic lymphocyte traffic.
Rat T cells labeled with
99mTc were injected in syngeneic animals, and their fate was studied by serial scintigraphic scanning. Sorted naïve CD4
+ CD45RC
bright T cells homed to lymphoid organs and accumulated in spleen. CD4
+ CD45RC
dim memory lymphocytes first reached the liver and the lungs and recirculated.
The results obtained by using the scintigraphic method to in vivo study the lymphocyte homing in rats are comparable to those obtained with previously used experimental methods. We consider the scintigraphic method a useful tool to in vivo track lymphocytes and to address therapeutic treatment in men.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>CD4 Lymphocyte Count - methods</subject><subject>CD4-Positive T-Lymphocytes - diagnostic imaging</subject><subject>CD4-Positive T-Lymphocytes - immunology</subject><subject>CD4-Positive T-Lymphocytes - physiology</subject><subject>Cell homing</subject><subject>Cell Movement - immunology</subject><subject>Cell Movement - physiology</subject><subject>Lymphoid Tissue - diagnostic imaging</subject><subject>Lymphoid Tissue - immunology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Memory cells</subject><subject>Naïve cells</subject><subject>Organ Specificity</subject><subject>Radionuclide Imaging</subject><subject>Radiopharmaceuticals</subject><subject>Rat</subject><subject>Rats</subject><subject>Rats, Inbred F344</subject><subject>Scintigraphy</subject><subject>T lymphocytes</subject><subject>T-Lymphocytes - diagnostic imaging</subject><subject>T-Lymphocytes - immunology</subject><subject>T-Lymphocytes - physiology</subject><subject>Technetium Tc 99m Exametazime</subject><issn>0969-8051</issn><issn>1872-9614</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtKAzEUhoMoWquPoGSj6GI0l5npxI1I8QYFF63rcJo500bmUpOZQn160wt26SqBfH_Ofz5CLji744yn92OmUhVlLOE3TN4yFi6ROiA9ng1EpFIeH5LeH3JCTr3_YiEXc3ZMTrhQjIlM9ch4gmZeY2u7KlKqot7YurUzB4v5irYNXVrfQWl_kE4ig2VJ501l6xm1dXhaNg8U6MKhKW1tDZTUt12-OiNHBZQez3dnn3y-PE-Gb9Ho4_V9-DSKjFS8jYokVnmWZqEr8Gw6UCDRgEHJBSopkCOkA85BQoyQGBAqjpmUcdhCFSCE7JPr7b8L13x36FtdWb8uCTU2ndcDGSYkXAUw2YLGNd47LPTC2QrcSnOm1zb1xqZeq9JM6o1Nvc5d7gZ00wrzfWqnLwBXOwB8WL9wUBvr91zCUqlEFrjHLYdBx9Ki08Ez1gZzG-S1Om_sP1V-AXSWkIA</recordid><startdate>20030801</startdate><enddate>20030801</enddate><creator>Fulgenzi, Alessandro</creator><creator>Ferrero, Elisabetta</creator><creator>Gasparini, Massimo</creator><creator>Casati, Rosangela</creator><creator>Riccardo Colombo, Fabio</creator><creator>Gerundini, Paolo</creator><creator>Elena Ferrero, Maria</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20030801</creationdate><title>Technetium-99m scintigraphy to visualize T-cell homing in vivo: a preclinical study</title><author>Fulgenzi, Alessandro ; Ferrero, Elisabetta ; Gasparini, Massimo ; Casati, Rosangela ; Riccardo Colombo, Fabio ; Gerundini, Paolo ; Elena Ferrero, Maria</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-f549d868614a18b79a3ecace312e932e1ea6711a3a4ea5ca294403340169fa223</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>CD4 Lymphocyte Count - methods</topic><topic>CD4-Positive T-Lymphocytes - diagnostic imaging</topic><topic>CD4-Positive T-Lymphocytes - immunology</topic><topic>CD4-Positive T-Lymphocytes - physiology</topic><topic>Cell homing</topic><topic>Cell Movement - immunology</topic><topic>Cell Movement - physiology</topic><topic>Lymphoid Tissue - diagnostic imaging</topic><topic>Lymphoid Tissue - immunology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Memory cells</topic><topic>Naïve cells</topic><topic>Organ Specificity</topic><topic>Radionuclide Imaging</topic><topic>Radiopharmaceuticals</topic><topic>Rat</topic><topic>Rats</topic><topic>Rats, Inbred F344</topic><topic>Scintigraphy</topic><topic>T lymphocytes</topic><topic>T-Lymphocytes - diagnostic imaging</topic><topic>T-Lymphocytes - immunology</topic><topic>T-Lymphocytes - physiology</topic><topic>Technetium Tc 99m Exametazime</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fulgenzi, Alessandro</creatorcontrib><creatorcontrib>Ferrero, Elisabetta</creatorcontrib><creatorcontrib>Gasparini, Massimo</creatorcontrib><creatorcontrib>Casati, Rosangela</creatorcontrib><creatorcontrib>Riccardo Colombo, Fabio</creatorcontrib><creatorcontrib>Gerundini, Paolo</creatorcontrib><creatorcontrib>Elena Ferrero, Maria</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Nuclear medicine and biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fulgenzi, Alessandro</au><au>Ferrero, Elisabetta</au><au>Gasparini, Massimo</au><au>Casati, Rosangela</au><au>Riccardo Colombo, Fabio</au><au>Gerundini, Paolo</au><au>Elena Ferrero, Maria</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Technetium-99m scintigraphy to visualize T-cell homing in vivo: a preclinical study</atitle><jtitle>Nuclear medicine and biology</jtitle><addtitle>Nucl Med Biol</addtitle><date>2003-08-01</date><risdate>2003</risdate><volume>30</volume><issue>6</issue><spage>633</spage><epage>642</epage><pages>633-642</pages><issn>0969-8051</issn><eissn>1872-9614</eissn><abstract>The knowledge of lymphocyte distribution is very usefulness in monitoring therapeutic treatments. We present here a method employed in clinical practice, the scintigraphy, to study in the rat the physiologic lymphocyte traffic.
Rat T cells labeled with
99mTc were injected in syngeneic animals, and their fate was studied by serial scintigraphic scanning. Sorted naïve CD4
+ CD45RC
bright T cells homed to lymphoid organs and accumulated in spleen. CD4
+ CD45RC
dim memory lymphocytes first reached the liver and the lungs and recirculated.
The results obtained by using the scintigraphic method to in vivo study the lymphocyte homing in rats are comparable to those obtained with previously used experimental methods. We consider the scintigraphic method a useful tool to in vivo track lymphocytes and to address therapeutic treatment in men.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>12900289</pmid><doi>10.1016/S0969-8051(03)00051-9</doi><tpages>10</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences CD4 Lymphocyte Count - methods CD4-Positive T-Lymphocytes - diagnostic imaging CD4-Positive T-Lymphocytes - immunology CD4-Positive T-Lymphocytes - physiology Cell homing Cell Movement - immunology Cell Movement - physiology Lymphoid Tissue - diagnostic imaging Lymphoid Tissue - immunology Male Medical sciences Memory cells Naïve cells Organ Specificity Radionuclide Imaging Radiopharmaceuticals Rat Rats Rats, Inbred F344 Scintigraphy T lymphocytes T-Lymphocytes - diagnostic imaging T-Lymphocytes - immunology T-Lymphocytes - physiology Technetium Tc 99m Exametazime |
| title | Technetium-99m scintigraphy to visualize T-cell homing in vivo: a preclinical study |
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