The structure of the capsular polysaccharide (K5 antigen) of urinary-tract-infective Escherichia coli 010:K5:H4. A polymer similar to desulfo-heparin
The capsular polysaccharide was isolated from Escherichia coli 010:K5:H4; it could not be obtained from a uncapsulated (K5-) mutant. It contains N-acetylglucosamine and glucuronic acid in a molar ratio of 1:1. Acid hydrolysis of the acidic polysaccharide as well as Smith degradation and degradation...
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| Veröffentlicht in: | European journal of biochemistry 1981-05, Vol.116 (2), p.359-364 |
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| container_end_page | 364 |
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| container_title | European journal of biochemistry |
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| creator | Vann, W F Schmidt, M A Jann, B Jann, K |
| description | The capsular polysaccharide was isolated from Escherichia coli 010:K5:H4; it could not be obtained from a uncapsulated (K5-) mutant. It contains N-acetylglucosamine and glucuronic acid in a molar ratio of 1:1. Acid hydrolysis of the acidic polysaccharide as well as Smith degradation and degradation by deamination of the carboxyl-reduced polysaccharide suggested that the polysaccharide is composed of a disaccharide repeating unit. The data obtained by methylation analysis and nuclear magnetic resonance spectroscopy indicated that the repeating sequence of the capsular polysaccharide is the 4-beta-glucuronyl-1,4-alpha-N-acetylglucosaminyl unit. This structure is similar to that of desulfo-heparin. |
| doi_str_mv | 10.1111/j.1432-1033.1981.tb05343.x |
| format | Article |
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This structure is similar to that of desulfo-heparin.</description><subject>Acetylation</subject><subject>Antigens - isolation & purification</subject><subject>Antigens, Bacterial</subject><subject>Antigens, Surface</subject><subject>Carbohydrate Sequence</subject><subject>Carbohydrates - analysis</subject><subject>Escherichia coli - immunology</subject><subject>Humans</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Mass Spectrometry</subject><subject>Methylation</subject><subject>Urinary Tract Infections - microbiology</subject><issn>0014-2956</issn><issn>1432-1033</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9Uctu1TAQtRCo3BY-AcligegiYRw7D99dVRWKWolNWVvOZEx8lcfFdlD7IfwvCb3qbEaaOQ_NHMY-CsjFWl8OuVCyyARImQvdiDy1UEol88dXbPeyes12AEJlhS6rt-w8xgMAVLqqz9hZDaLRoHfs70NPPKawYFoC8dnxtA7QHuMy2MCP8_AULWJvg--If74ruZ2S_0XT5YZdgp9seMpSsJgyPznC5P8Qv4nYU_DYe8txHjwHAfu7cn-rcn71X3SkwKMf_WaSZt7R6ufmrKfj6jS9Y2-cHSK9P_UL9vPrzcP1bXb_49v366v7DNcjU1ZI0tBK0NAJUTtdCNRt3ShoOuqcK6Com4qc1YpEKVyLTVEqxMYq7BwVKC_Yp2fdY5h_LxSTGX1EGgY70bxEU8tSVVrpFbh_BmKYYwzkzDH4cT3dCDBbJuZgtseb7fFmy8ScMjGPK_nDyWVpR-peqKcQ5D8nBorc</recordid><startdate>19810515</startdate><enddate>19810515</enddate><creator>Vann, W F</creator><creator>Schmidt, M A</creator><creator>Jann, B</creator><creator>Jann, K</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19810515</creationdate><title>The structure of the capsular polysaccharide (K5 antigen) of urinary-tract-infective Escherichia coli 010:K5:H4. 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| identifier | ISSN: 0014-2956 |
| ispartof | European journal of biochemistry, 1981-05, Vol.116 (2), p.359-364 |
| issn | 0014-2956 1432-1033 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73546949 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Acetylation Antigens - isolation & purification Antigens, Bacterial Antigens, Surface Carbohydrate Sequence Carbohydrates - analysis Escherichia coli - immunology Humans Magnetic Resonance Spectroscopy Mass Spectrometry Methylation Urinary Tract Infections - microbiology |
| title | The structure of the capsular polysaccharide (K5 antigen) of urinary-tract-infective Escherichia coli 010:K5:H4. A polymer similar to desulfo-heparin |
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