Detection of Escherichia coli serogroups O26, O103, O111 and O145 from bovine faeces using immunomagnetic separation and PCR/DNA probe techniques

Aims: The aim of this study was to isolate Escherichia coli O26, O103, O111 and O145 from 745 samples of bovine faeces using (i) immunomagnetic separation (IMS) beads coated with antibodies to lipopolysaccharide, and slide agglutination (SA) tests and (ii) PCR and DNA probes for the detection of the...

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Veröffentlicht in:	Letters in applied microbiology 2003-01, Vol.37 (3), p.207-212
Hauptverfasser:	Jenkins, C., Pearce, M.C., Smith, A.W., Knight, H.I., Shaw, D.J., Cheasty, T., Foster, G., Gunn, G.J., Dougan, G., Smith, H.R., Frankel, G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Agglutination Tests - methods Animals Cattle Cattle Diseases - microbiology DNA Probes - genetics E. coli Escherichia coli - classification Escherichia coli - genetics Escherichia coli - isolation & purification Escherichia coli Infections - microbiology Escherichia coli Infections - veterinary Feces - microbiology Female Immunomagnetic Separation - methods IMS PCR/DNA probes Polymerase Chain Reaction - methods Sensitivity and Specificity Serotyping Shiga Toxins - genetics VTEC
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