Identification of eight members of the Argonaute family in the human genome
A number of genes have been identified as members of the Argonaute family in various nonhuman organisms and these genes are considered to play important roles in the development and maintenance of germ-line stem cells. In this study, we identified the human Argonaute family, consisting of eight memb...
Gespeichert in:
| Veröffentlicht in: | Genomics (San Diego, Calif.) Calif.), 2003-09, Vol.82 (3), p.323-330 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 330 |
|---|---|
| container_issue | 3 |
| container_start_page | 323 |
| container_title | Genomics (San Diego, Calif.) |
| container_volume | 82 |
| creator | Sasaki, Takashi Shiohama, Aiko Minoshima, Shinsei Shimizu, Nobuyoshi |
| description | A number of genes have been identified as members of the Argonaute family in various nonhuman organisms and these genes are considered to play important roles in the development and maintenance of germ-line stem cells. In this study, we identified the human Argonaute family, consisting of eight members. Proteins to be produced from these family members retain a common architecture with the PAZ motif in the middle and Piwi motif in the C-terminal region. Based on the sequence comparison, eight members of the Argonaute family were classified into two subfamilies: the PIWI subfamily (
PIWIL1/HIWI, PIWIL2/HILI, PIWIL3, and
PIWIL4/HIWI2) and the eIF2C/AGO subfamily (
EIF2C1/hAGO1, EIF2C2/hAGO2, EIF2C3/hAGO3, and
EIF2C4/hAGO4). PCR analysis using human multitissue cDNA panels indicated that all four members of the PIWI subfamily are expressed mainly in the testis, whereas all four members of the eIF2C/AGO subfamily are expressed in a variety of adult tissues. Immunoprecipitation and affinity binding experiments using human HEK293 cells cotransfected with cDNAs for FLAG-tagged DICER, a member of the ribonuclease III family, and the His-tagged members of the Argonaute family suggested that the proteins from members of both subfamilies are associated with DICER. We postulate that at least some members of the human Argonaute family may be involved in the development and maintenance of stem cells through the RNA-mediated gene-quelling mechanisms associated with DICER. |
| doi_str_mv | 10.1016/S0888-7543(03)00129-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73541665</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0888754303001290</els_id><sourcerecordid>73541665</sourcerecordid><originalsourceid>FETCH-LOGICAL-c540t-9901056cbce98f80ed8a78e4a551b02d1981ee43388d9e4547a34063f7bd5ed33</originalsourceid><addsrcrecordid>eNqFkN9r2zAQgMXoWNJ0f8KKX1raB6-nSLLPT6OUtisr7GHbs5Clc6Jhy5lkD_Lf12lC-xh0oOP47gcfY184fOXAi5tfgIh5qaS4AnENwJdVDh_YnANWORayOGHzN2TGTlP6CwCVwOUnNptgKFCVc_bjyVEYfOOtGXwfsr7JyK_WQ9ZRV1NMu8Kwpuw2rvpgxoGyxnS-3WY-vNbXY2dCtqLQd3TGPjamTfT58C_Yn4f733ff8-efj093t8-5VRKGvKqAgypsbanCBoEcmhJJGqV4DUvHK-REUghEV5FUsjRCQiGasnaKnBALdrmfu4n9v5HSoDufLLWtCdSPSZdCSV4U6ijIEZfTKydQ7UEb-5QiNXoTfWfiVnPQO936VbfeudQwxU73lCzY-WHBWHfk3rsOfifg4gCYZE3bRBOsT--cAlGCwon7tudo8vbfU9TJegqWnI9kB-16f-SUF9hqmws</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>18828287</pqid></control><display><type>article</type><title>Identification of eight members of the Argonaute family in the human genome</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Sasaki, Takashi ; Shiohama, Aiko ; Minoshima, Shinsei ; Shimizu, Nobuyoshi</creator><creatorcontrib>Sasaki, Takashi ; Shiohama, Aiko ; Minoshima, Shinsei ; Shimizu, Nobuyoshi</creatorcontrib><description>A number of genes have been identified as members of the Argonaute family in various nonhuman organisms and these genes are considered to play important roles in the development and maintenance of germ-line stem cells. In this study, we identified the human Argonaute family, consisting of eight members. Proteins to be produced from these family members retain a common architecture with the PAZ motif in the middle and Piwi motif in the C-terminal region. Based on the sequence comparison, eight members of the Argonaute family were classified into two subfamilies: the PIWI subfamily (
PIWIL1/HIWI, PIWIL2/HILI, PIWIL3, and
PIWIL4/HIWI2) and the eIF2C/AGO subfamily (
EIF2C1/hAGO1, EIF2C2/hAGO2, EIF2C3/hAGO3, and
EIF2C4/hAGO4). PCR analysis using human multitissue cDNA panels indicated that all four members of the PIWI subfamily are expressed mainly in the testis, whereas all four members of the eIF2C/AGO subfamily are expressed in a variety of adult tissues. Immunoprecipitation and affinity binding experiments using human HEK293 cells cotransfected with cDNAs for FLAG-tagged DICER, a member of the ribonuclease III family, and the His-tagged members of the Argonaute family suggested that the proteins from members of both subfamilies are associated with DICER. We postulate that at least some members of the human Argonaute family may be involved in the development and maintenance of stem cells through the RNA-mediated gene-quelling mechanisms associated with DICER.</description><identifier>ISSN: 0888-7543</identifier><identifier>EISSN: 1089-8646</identifier><identifier>DOI: 10.1016/S0888-7543(03)00129-0</identifier><identifier>PMID: 12906857</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>AGO ; Amino Acid Motifs ; Argonaute Proteins ; Biological and medical sciences ; Cell physiology ; eIF2C ; Eukaryotic Initiation Factor-2 ; Eukaryotic Initiation Factors - genetics ; Eukaryotic Initiation Factors - immunology ; Fundamental and applied biological sciences. Psychology ; Genome, Human ; HIWI ; Humans ; Molecular and cellular biology ; Molecular Sequence Data ; Organ Specificity ; PAZ motif ; Peptide Initiation Factors - genetics ; Peptide Initiation Factors - immunology ; PIWI ; Piwi motif ; Precipitin Tests ; Proteins - genetics ; Proteins - immunology ; PTGS ; Ribonuclease III - immunology ; RNAi ; Signal transduction ; Stem cell</subject><ispartof>Genomics (San Diego, Calif.), 2003-09, Vol.82 (3), p.323-330</ispartof><rights>2003 Elsevier Science (USA)</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c540t-9901056cbce98f80ed8a78e4a551b02d1981ee43388d9e4547a34063f7bd5ed33</citedby><cites>FETCH-LOGICAL-c540t-9901056cbce98f80ed8a78e4a551b02d1981ee43388d9e4547a34063f7bd5ed33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0888754303001290$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15037058$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12906857$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sasaki, Takashi</creatorcontrib><creatorcontrib>Shiohama, Aiko</creatorcontrib><creatorcontrib>Minoshima, Shinsei</creatorcontrib><creatorcontrib>Shimizu, Nobuyoshi</creatorcontrib><title>Identification of eight members of the Argonaute family in the human genome</title><title>Genomics (San Diego, Calif.)</title><addtitle>Genomics</addtitle><description>A number of genes have been identified as members of the Argonaute family in various nonhuman organisms and these genes are considered to play important roles in the development and maintenance of germ-line stem cells. In this study, we identified the human Argonaute family, consisting of eight members. Proteins to be produced from these family members retain a common architecture with the PAZ motif in the middle and Piwi motif in the C-terminal region. Based on the sequence comparison, eight members of the Argonaute family were classified into two subfamilies: the PIWI subfamily (
PIWIL1/HIWI, PIWIL2/HILI, PIWIL3, and
PIWIL4/HIWI2) and the eIF2C/AGO subfamily (
EIF2C1/hAGO1, EIF2C2/hAGO2, EIF2C3/hAGO3, and
EIF2C4/hAGO4). PCR analysis using human multitissue cDNA panels indicated that all four members of the PIWI subfamily are expressed mainly in the testis, whereas all four members of the eIF2C/AGO subfamily are expressed in a variety of adult tissues. Immunoprecipitation and affinity binding experiments using human HEK293 cells cotransfected with cDNAs for FLAG-tagged DICER, a member of the ribonuclease III family, and the His-tagged members of the Argonaute family suggested that the proteins from members of both subfamilies are associated with DICER. We postulate that at least some members of the human Argonaute family may be involved in the development and maintenance of stem cells through the RNA-mediated gene-quelling mechanisms associated with DICER.</description><subject>AGO</subject><subject>Amino Acid Motifs</subject><subject>Argonaute Proteins</subject><subject>Biological and medical sciences</subject><subject>Cell physiology</subject><subject>eIF2C</subject><subject>Eukaryotic Initiation Factor-2</subject><subject>Eukaryotic Initiation Factors - genetics</subject><subject>Eukaryotic Initiation Factors - immunology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genome, Human</subject><subject>HIWI</subject><subject>Humans</subject><subject>Molecular and cellular biology</subject><subject>Molecular Sequence Data</subject><subject>Organ Specificity</subject><subject>PAZ motif</subject><subject>Peptide Initiation Factors - genetics</subject><subject>Peptide Initiation Factors - immunology</subject><subject>PIWI</subject><subject>Piwi motif</subject><subject>Precipitin Tests</subject><subject>Proteins - genetics</subject><subject>Proteins - immunology</subject><subject>PTGS</subject><subject>Ribonuclease III - immunology</subject><subject>RNAi</subject><subject>Signal transduction</subject><subject>Stem cell</subject><issn>0888-7543</issn><issn>1089-8646</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkN9r2zAQgMXoWNJ0f8KKX1raB6-nSLLPT6OUtisr7GHbs5Clc6Jhy5lkD_Lf12lC-xh0oOP47gcfY184fOXAi5tfgIh5qaS4AnENwJdVDh_YnANWORayOGHzN2TGTlP6CwCVwOUnNptgKFCVc_bjyVEYfOOtGXwfsr7JyK_WQ9ZRV1NMu8Kwpuw2rvpgxoGyxnS-3WY-vNbXY2dCtqLQd3TGPjamTfT58C_Yn4f733ff8-efj093t8-5VRKGvKqAgypsbanCBoEcmhJJGqV4DUvHK-REUghEV5FUsjRCQiGasnaKnBALdrmfu4n9v5HSoDufLLWtCdSPSZdCSV4U6ijIEZfTKydQ7UEb-5QiNXoTfWfiVnPQO936VbfeudQwxU73lCzY-WHBWHfk3rsOfifg4gCYZE3bRBOsT--cAlGCwon7tudo8vbfU9TJegqWnI9kB-16f-SUF9hqmws</recordid><startdate>20030901</startdate><enddate>20030901</enddate><creator>Sasaki, Takashi</creator><creator>Shiohama, Aiko</creator><creator>Minoshima, Shinsei</creator><creator>Shimizu, Nobuyoshi</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20030901</creationdate><title>Identification of eight members of the Argonaute family in the human genome</title><author>Sasaki, Takashi ; Shiohama, Aiko ; Minoshima, Shinsei ; Shimizu, Nobuyoshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c540t-9901056cbce98f80ed8a78e4a551b02d1981ee43388d9e4547a34063f7bd5ed33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>AGO</topic><topic>Amino Acid Motifs</topic><topic>Argonaute Proteins</topic><topic>Biological and medical sciences</topic><topic>Cell physiology</topic><topic>eIF2C</topic><topic>Eukaryotic Initiation Factor-2</topic><topic>Eukaryotic Initiation Factors - genetics</topic><topic>Eukaryotic Initiation Factors - immunology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genome, Human</topic><topic>HIWI</topic><topic>Humans</topic><topic>Molecular and cellular biology</topic><topic>Molecular Sequence Data</topic><topic>Organ Specificity</topic><topic>PAZ motif</topic><topic>Peptide Initiation Factors - genetics</topic><topic>Peptide Initiation Factors - immunology</topic><topic>PIWI</topic><topic>Piwi motif</topic><topic>Precipitin Tests</topic><topic>Proteins - genetics</topic><topic>Proteins - immunology</topic><topic>PTGS</topic><topic>Ribonuclease III - immunology</topic><topic>RNAi</topic><topic>Signal transduction</topic><topic>Stem cell</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sasaki, Takashi</creatorcontrib><creatorcontrib>Shiohama, Aiko</creatorcontrib><creatorcontrib>Minoshima, Shinsei</creatorcontrib><creatorcontrib>Shimizu, Nobuyoshi</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Genomics (San Diego, Calif.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sasaki, Takashi</au><au>Shiohama, Aiko</au><au>Minoshima, Shinsei</au><au>Shimizu, Nobuyoshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of eight members of the Argonaute family in the human genome</atitle><jtitle>Genomics (San Diego, Calif.)</jtitle><addtitle>Genomics</addtitle><date>2003-09-01</date><risdate>2003</risdate><volume>82</volume><issue>3</issue><spage>323</spage><epage>330</epage><pages>323-330</pages><issn>0888-7543</issn><eissn>1089-8646</eissn><abstract>A number of genes have been identified as members of the Argonaute family in various nonhuman organisms and these genes are considered to play important roles in the development and maintenance of germ-line stem cells. In this study, we identified the human Argonaute family, consisting of eight members. Proteins to be produced from these family members retain a common architecture with the PAZ motif in the middle and Piwi motif in the C-terminal region. Based on the sequence comparison, eight members of the Argonaute family were classified into two subfamilies: the PIWI subfamily (
PIWIL1/HIWI, PIWIL2/HILI, PIWIL3, and
PIWIL4/HIWI2) and the eIF2C/AGO subfamily (
EIF2C1/hAGO1, EIF2C2/hAGO2, EIF2C3/hAGO3, and
EIF2C4/hAGO4). PCR analysis using human multitissue cDNA panels indicated that all four members of the PIWI subfamily are expressed mainly in the testis, whereas all four members of the eIF2C/AGO subfamily are expressed in a variety of adult tissues. Immunoprecipitation and affinity binding experiments using human HEK293 cells cotransfected with cDNAs for FLAG-tagged DICER, a member of the ribonuclease III family, and the His-tagged members of the Argonaute family suggested that the proteins from members of both subfamilies are associated with DICER. We postulate that at least some members of the human Argonaute family may be involved in the development and maintenance of stem cells through the RNA-mediated gene-quelling mechanisms associated with DICER.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>12906857</pmid><doi>10.1016/S0888-7543(03)00129-0</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0888-7543 |
| ispartof | Genomics (San Diego, Calif.), 2003-09, Vol.82 (3), p.323-330 |
| issn | 0888-7543 1089-8646 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73541665 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | AGO Amino Acid Motifs Argonaute Proteins Biological and medical sciences Cell physiology eIF2C Eukaryotic Initiation Factor-2 Eukaryotic Initiation Factors - genetics Eukaryotic Initiation Factors - immunology Fundamental and applied biological sciences. Psychology Genome, Human HIWI Humans Molecular and cellular biology Molecular Sequence Data Organ Specificity PAZ motif Peptide Initiation Factors - genetics Peptide Initiation Factors - immunology PIWI Piwi motif Precipitin Tests Proteins - genetics Proteins - immunology PTGS Ribonuclease III - immunology RNAi Signal transduction Stem cell |
| title | Identification of eight members of the Argonaute family in the human genome |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-04T20%3A04%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Identification%20of%20eight%20members%20of%20the%20Argonaute%20family%20in%20the%20human%20genome&rft.jtitle=Genomics%20(San%20Diego,%20Calif.)&rft.au=Sasaki,%20Takashi&rft.date=2003-09-01&rft.volume=82&rft.issue=3&rft.spage=323&rft.epage=330&rft.pages=323-330&rft.issn=0888-7543&rft.eissn=1089-8646&rft_id=info:doi/10.1016/S0888-7543(03)00129-0&rft_dat=%3Cproquest_cross%3E73541665%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=18828287&rft_id=info:pmid/12906857&rft_els_id=S0888754303001290&rfr_iscdi=true |