Investigating a new cryopreservation protocol for yams (Dioscorea spp.)
Experiments were initiated to develop protocols for four species of Dioscorea, namely D. bulbifera L., D. oppositifolia L., D. alata L. and D. cayenensis Lam., and to assess the effects of various factors on the survival and regrowth of explants after rewarming. The treatments consisted of three dif...
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| Veröffentlicht in: | Plant cell reports 2003-08, Vol.21 (12), p.1159-1166 |
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| creator | LEUNUFNA, S KELLER, E. R. J |
| description | Experiments were initiated to develop protocols for four species of Dioscorea, namely D. bulbifera L., D. oppositifolia L., D. alata L. and D. cayenensis Lam., and to assess the effects of various factors on the survival and regrowth of explants after rewarming. The treatments consisted of three different methods-vitrification, droplet and a modified droplet method-as well as the following variables-cryoprotective solution, sucrose concentration in the preadaptation medium and cold acclimation. While most of the protocols resulted in low to zero survival and regrowth rates, maximum survival rates of 100% and 75% were obtained for D. oppositifolia L. and D. cayenensis Lam., respectively, using two different protocols of the modified droplet method. A higher average survival rate was obtained using the droplet and modified droplet techniques than for the original vitrification methods. When the average of all the experiments was taken, more than one-half (52%) of the surviving explants developed further within 1 month with the modified droplet method compared to zero regrowth with the two other methods. The optimum protocol for cryopreservation is specific for each genotype. |
| doi_str_mv | 10.1007/s00299-003-0652-3 |
| format | Article |
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R. J</creator><creatorcontrib>LEUNUFNA, S ; KELLER, E. R. J</creatorcontrib><description>Experiments were initiated to develop protocols for four species of Dioscorea, namely D. bulbifera L., D. oppositifolia L., D. alata L. and D. cayenensis Lam., and to assess the effects of various factors on the survival and regrowth of explants after rewarming. The treatments consisted of three different methods-vitrification, droplet and a modified droplet method-as well as the following variables-cryoprotective solution, sucrose concentration in the preadaptation medium and cold acclimation. While most of the protocols resulted in low to zero survival and regrowth rates, maximum survival rates of 100% and 75% were obtained for D. oppositifolia L. and D. cayenensis Lam., respectively, using two different protocols of the modified droplet method. A higher average survival rate was obtained using the droplet and modified droplet techniques than for the original vitrification methods. When the average of all the experiments was taken, more than one-half (52%) of the surviving explants developed further within 1 month with the modified droplet method compared to zero regrowth with the two other methods. The optimum protocol for cryopreservation is specific for each genotype.</description><identifier>ISSN: 0721-7714</identifier><identifier>EISSN: 1432-203X</identifier><identifier>DOI: 10.1007/s00299-003-0652-3</identifier><identifier>PMID: 12819924</identifier><identifier>CODEN: PCRPD8</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Acclimatization ; Agronomy. Soil science and plant productions ; Biological and medical sciences ; Cryopreservation - methods ; Dioscorea ; Dioscorea alata ; Dioscorea bulbifera ; Dioscorea cayenensis ; Dioscorea oppositifolia ; Fundamental and applied biological sciences. Psychology ; Generalities. Genetics. 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R. J</creatorcontrib><title>Investigating a new cryopreservation protocol for yams (Dioscorea spp.)</title><title>Plant cell reports</title><addtitle>Plant Cell Rep</addtitle><description>Experiments were initiated to develop protocols for four species of Dioscorea, namely D. bulbifera L., D. oppositifolia L., D. alata L. and D. cayenensis Lam., and to assess the effects of various factors on the survival and regrowth of explants after rewarming. The treatments consisted of three different methods-vitrification, droplet and a modified droplet method-as well as the following variables-cryoprotective solution, sucrose concentration in the preadaptation medium and cold acclimation. While most of the protocols resulted in low to zero survival and regrowth rates, maximum survival rates of 100% and 75% were obtained for D. oppositifolia L. and D. cayenensis Lam., respectively, using two different protocols of the modified droplet method. A higher average survival rate was obtained using the droplet and modified droplet techniques than for the original vitrification methods. When the average of all the experiments was taken, more than one-half (52%) of the surviving explants developed further within 1 month with the modified droplet method compared to zero regrowth with the two other methods. The optimum protocol for cryopreservation is specific for each genotype.</description><subject>Acclimatization</subject><subject>Agronomy. Soil science and plant productions</subject><subject>Biological and medical sciences</subject><subject>Cryopreservation - methods</subject><subject>Dioscorea</subject><subject>Dioscorea alata</subject><subject>Dioscorea bulbifera</subject><subject>Dioscorea cayenensis</subject><subject>Dioscorea oppositifolia</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Generalities. Genetics. 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R. J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Investigating a new cryopreservation protocol for yams (Dioscorea spp.)</atitle><jtitle>Plant cell reports</jtitle><addtitle>Plant Cell Rep</addtitle><date>2003-08-01</date><risdate>2003</risdate><volume>21</volume><issue>12</issue><spage>1159</spage><epage>1166</epage><pages>1159-1166</pages><issn>0721-7714</issn><eissn>1432-203X</eissn><coden>PCRPD8</coden><abstract>Experiments were initiated to develop protocols for four species of Dioscorea, namely D. bulbifera L., D. oppositifolia L., D. alata L. and D. cayenensis Lam., and to assess the effects of various factors on the survival and regrowth of explants after rewarming. The treatments consisted of three different methods-vitrification, droplet and a modified droplet method-as well as the following variables-cryoprotective solution, sucrose concentration in the preadaptation medium and cold acclimation. While most of the protocols resulted in low to zero survival and regrowth rates, maximum survival rates of 100% and 75% were obtained for D. oppositifolia L. and D. cayenensis Lam., respectively, using two different protocols of the modified droplet method. A higher average survival rate was obtained using the droplet and modified droplet techniques than for the original vitrification methods. When the average of all the experiments was taken, more than one-half (52%) of the surviving explants developed further within 1 month with the modified droplet method compared to zero regrowth with the two other methods. The optimum protocol for cryopreservation is specific for each genotype.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>12819924</pmid><doi>10.1007/s00299-003-0652-3</doi><tpages>8</tpages></addata></record> |
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| subjects | Acclimatization Agronomy. Soil science and plant productions Biological and medical sciences Cryopreservation - methods Dioscorea Dioscorea alata Dioscorea bulbifera Dioscorea cayenensis Dioscorea oppositifolia Fundamental and applied biological sciences. Psychology Generalities. Genetics. Plant material Genetic resources, diversity Genetics and breeding of economic plants Methods Plant material Regrowth Survival |
| title | Investigating a new cryopreservation protocol for yams (Dioscorea spp.) |
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