SODIUM-DEPENDENT RELAXATION IN ARTERIAL SMOOTH MUSCLE
SUMMARY 1. The effects of isosmotic substitution of choline for sodium on resting tension and on relaxation after noradrenaline‐induced contraction was studied in rabbit isolated aortic strips immersed in Hepes‐buffered physiologic salt solution (PSS) warmed to 37°C and gassed with 100% O2. 2. Isosm...
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| Veröffentlicht in: | Clinical and experimental pharmacology & physiology 1981-06, Vol.8 (3), p.209-214 |
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| container_title | Clinical and experimental pharmacology & physiology |
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| creator | Gillespie, Mark N. Niehaus, Karl E. Rodger, Ian W. Diamond, Louis |
| description | SUMMARY
1. The effects of isosmotic substitution of choline for sodium on resting tension and on relaxation after noradrenaline‐induced contraction was studied in rabbit isolated aortic strips immersed in Hepes‐buffered physiologic salt solution (PSS) warmed to 37°C and gassed with 100% O2.
2. Isosmotic substitution of choline for sodium produced a sustained increase in resting tension which effectively prevented any evaluation of the influence of sodium on relaxation. The increase in resting tension was insensitive to 10−8 mol/1 atropine but was abolished by 10 min exposure to calcium‐free PSS prior to replacement of sodium.
3. Under sodium‐calcium free conditions which eliminated the increase in resting tension observed in sodium‐free PSS, stimulation with 10−5 mol/1 noradrenaline initiated contractions that were 55 ± 7.5% of the control response in normal PSS. Washout of noradrenaline with sodium‐calcium‐free PSS failed to produce any decrement in tension. However, restoration of the normal sodium resulted in gradual relaxation.
4. These results suggest that sodium is required for relaxation after noradrenaline‐induced contraction of arterial smooth muscle. |
| doi_str_mv | 10.1111/j.1440-1681.1981.tb00153.x |
| format | Article |
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1. The effects of isosmotic substitution of choline for sodium on resting tension and on relaxation after noradrenaline‐induced contraction was studied in rabbit isolated aortic strips immersed in Hepes‐buffered physiologic salt solution (PSS) warmed to 37°C and gassed with 100% O2.
2. Isosmotic substitution of choline for sodium produced a sustained increase in resting tension which effectively prevented any evaluation of the influence of sodium on relaxation. The increase in resting tension was insensitive to 10−8 mol/1 atropine but was abolished by 10 min exposure to calcium‐free PSS prior to replacement of sodium.
3. Under sodium‐calcium free conditions which eliminated the increase in resting tension observed in sodium‐free PSS, stimulation with 10−5 mol/1 noradrenaline initiated contractions that were 55 ± 7.5% of the control response in normal PSS. Washout of noradrenaline with sodium‐calcium‐free PSS failed to produce any decrement in tension. However, restoration of the normal sodium resulted in gradual relaxation.
4. These results suggest that sodium is required for relaxation after noradrenaline‐induced contraction of arterial smooth muscle.</description><identifier>ISSN: 0305-1870</identifier><identifier>EISSN: 1440-1681</identifier><identifier>DOI: 10.1111/j.1440-1681.1981.tb00153.x</identifier><identifier>PMID: 7249409</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; Aorta, Thoracic - physiology ; calcium ; Calcium - metabolism ; In Vitro Techniques ; Male ; Muscle Contraction - drug effects ; Muscle Relaxation - drug effects ; Muscle, Smooth, Vascular - physiology ; noradrenaline ; Norepinephrine - pharmacology ; Rabbits ; relaxation ; sodium ; Sodium - pharmacology ; vascular smooth muscle</subject><ispartof>Clinical and experimental pharmacology & physiology, 1981-06, Vol.8 (3), p.209-214</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3509-ff8a7b2dd812dca1a142f1dcb4dab5141c0e046b9f7653375619f66ce6bb35dd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1440-1681.1981.tb00153.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1440-1681.1981.tb00153.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7249409$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gillespie, Mark N.</creatorcontrib><creatorcontrib>Niehaus, Karl E.</creatorcontrib><creatorcontrib>Rodger, Ian W.</creatorcontrib><creatorcontrib>Diamond, Louis</creatorcontrib><title>SODIUM-DEPENDENT RELAXATION IN ARTERIAL SMOOTH MUSCLE</title><title>Clinical and experimental pharmacology & physiology</title><addtitle>Clin Exp Pharmacol Physiol</addtitle><description>SUMMARY
1. The effects of isosmotic substitution of choline for sodium on resting tension and on relaxation after noradrenaline‐induced contraction was studied in rabbit isolated aortic strips immersed in Hepes‐buffered physiologic salt solution (PSS) warmed to 37°C and gassed with 100% O2.
2. Isosmotic substitution of choline for sodium produced a sustained increase in resting tension which effectively prevented any evaluation of the influence of sodium on relaxation. The increase in resting tension was insensitive to 10−8 mol/1 atropine but was abolished by 10 min exposure to calcium‐free PSS prior to replacement of sodium.
3. Under sodium‐calcium free conditions which eliminated the increase in resting tension observed in sodium‐free PSS, stimulation with 10−5 mol/1 noradrenaline initiated contractions that were 55 ± 7.5% of the control response in normal PSS. Washout of noradrenaline with sodium‐calcium‐free PSS failed to produce any decrement in tension. However, restoration of the normal sodium resulted in gradual relaxation.
4. These results suggest that sodium is required for relaxation after noradrenaline‐induced contraction of arterial smooth muscle.</description><subject>Animals</subject><subject>Aorta, Thoracic - physiology</subject><subject>calcium</subject><subject>Calcium - metabolism</subject><subject>In Vitro Techniques</subject><subject>Male</subject><subject>Muscle Contraction - drug effects</subject><subject>Muscle Relaxation - drug effects</subject><subject>Muscle, Smooth, Vascular - physiology</subject><subject>noradrenaline</subject><subject>Norepinephrine - pharmacology</subject><subject>Rabbits</subject><subject>relaxation</subject><subject>sodium</subject><subject>Sodium - pharmacology</subject><subject>vascular smooth muscle</subject><issn>0305-1870</issn><issn>1440-1681</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkE1PwkAQhjdGg4j-BJPGg7fiTvejrQeTBipUS9tAUW-bfmwTEAS7EOHf29qGu3PYObwzz2QfhO4A96Gqh2UfKMU6cAv6YFfPLsUYGOkfzlD3FJ2jLiaY6WCZ-BJdKbXEGDPMSQd1TIPaFNtdxGbh0JtP9KEbucHQDWJt6vrOhxN7YaB5geZMY3fqOb42m4RhPNYm89nAd6_RRZGslLxpew_Nn914MNb9cOQNHF_PCMO2XhRWYqZGnltg5FkCCVCjgDxLaZ6kDChkWGLKU7swOSPEZBzsgvNM8jQlLM9JD9033G25-d5LtRPrhcrkapV8yc1eCZMww2JgVYOPzWBWbpQqZSG25WKdlEcBWNTOxFLUYkQtRtTOROtMHKrl2_bKPl3L_LTaSqrypyb_Wazk8R9kMXAj4w-gN4CF2snDCZCUn4Kb1bfFezAS9NV_id4sQ0TkF96dhXY</recordid><startdate>198106</startdate><enddate>198106</enddate><creator>Gillespie, Mark N.</creator><creator>Niehaus, Karl E.</creator><creator>Rodger, Ian W.</creator><creator>Diamond, Louis</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198106</creationdate><title>SODIUM-DEPENDENT RELAXATION IN ARTERIAL SMOOTH MUSCLE</title><author>Gillespie, Mark N. ; Niehaus, Karl E. ; Rodger, Ian W. ; Diamond, Louis</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3509-ff8a7b2dd812dca1a142f1dcb4dab5141c0e046b9f7653375619f66ce6bb35dd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1981</creationdate><topic>Animals</topic><topic>Aorta, Thoracic - physiology</topic><topic>calcium</topic><topic>Calcium - metabolism</topic><topic>In Vitro Techniques</topic><topic>Male</topic><topic>Muscle Contraction - drug effects</topic><topic>Muscle Relaxation - drug effects</topic><topic>Muscle, Smooth, Vascular - physiology</topic><topic>noradrenaline</topic><topic>Norepinephrine - pharmacology</topic><topic>Rabbits</topic><topic>relaxation</topic><topic>sodium</topic><topic>Sodium - pharmacology</topic><topic>vascular smooth muscle</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gillespie, Mark N.</creatorcontrib><creatorcontrib>Niehaus, Karl E.</creatorcontrib><creatorcontrib>Rodger, Ian W.</creatorcontrib><creatorcontrib>Diamond, Louis</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical and experimental pharmacology & physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gillespie, Mark N.</au><au>Niehaus, Karl E.</au><au>Rodger, Ian W.</au><au>Diamond, Louis</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>SODIUM-DEPENDENT RELAXATION IN ARTERIAL SMOOTH MUSCLE</atitle><jtitle>Clinical and experimental pharmacology & physiology</jtitle><addtitle>Clin Exp Pharmacol Physiol</addtitle><date>1981-06</date><risdate>1981</risdate><volume>8</volume><issue>3</issue><spage>209</spage><epage>214</epage><pages>209-214</pages><issn>0305-1870</issn><eissn>1440-1681</eissn><abstract>SUMMARY
1. The effects of isosmotic substitution of choline for sodium on resting tension and on relaxation after noradrenaline‐induced contraction was studied in rabbit isolated aortic strips immersed in Hepes‐buffered physiologic salt solution (PSS) warmed to 37°C and gassed with 100% O2.
2. Isosmotic substitution of choline for sodium produced a sustained increase in resting tension which effectively prevented any evaluation of the influence of sodium on relaxation. The increase in resting tension was insensitive to 10−8 mol/1 atropine but was abolished by 10 min exposure to calcium‐free PSS prior to replacement of sodium.
3. Under sodium‐calcium free conditions which eliminated the increase in resting tension observed in sodium‐free PSS, stimulation with 10−5 mol/1 noradrenaline initiated contractions that were 55 ± 7.5% of the control response in normal PSS. Washout of noradrenaline with sodium‐calcium‐free PSS failed to produce any decrement in tension. However, restoration of the normal sodium resulted in gradual relaxation.
4. These results suggest that sodium is required for relaxation after noradrenaline‐induced contraction of arterial smooth muscle.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>7249409</pmid><doi>10.1111/j.1440-1681.1981.tb00153.x</doi><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 0305-1870 |
| ispartof | Clinical and experimental pharmacology & physiology, 1981-06, Vol.8 (3), p.209-214 |
| issn | 0305-1870 1440-1681 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Aorta, Thoracic - physiology calcium Calcium - metabolism In Vitro Techniques Male Muscle Contraction - drug effects Muscle Relaxation - drug effects Muscle, Smooth, Vascular - physiology noradrenaline Norepinephrine - pharmacology Rabbits relaxation sodium Sodium - pharmacology vascular smooth muscle |
| title | SODIUM-DEPENDENT RELAXATION IN ARTERIAL SMOOTH MUSCLE |
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