Analysis of intracellular distribution and apoptosis involvement of the Ufd1l gene product by over-expression studies
UFD1L is the human homologue of the yeast ubiquitin fusion degradation 1 (Ufd1) gene and maps on chromosome 22q11.2 in the typically deleted region (TDR) for DiGeorge/velocardiofacial syndromes (DGS/VCFS). In yeast, Ufd1 protein is involved in a degradation pathway for ubiquitin fused products (UFD...
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| Veröffentlicht in: | Cell biochemistry and function 2003-09, Vol.21 (3), p.263-267 |
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| creator | Amati, Francesca Condò, Ivano Conti, Emanuela Sangiuolo, Federica Dallapiccola, Bruno Testi, Roberto Novelli, Giuseppe |
| description | UFD1L is the human homologue of the yeast ubiquitin fusion degradation 1 (Ufd1) gene and maps on chromosome 22q11.2 in the typically deleted region (TDR) for DiGeorge/velocardiofacial syndromes (DGS/VCFS). In yeast, Ufd1 protein is involved in a degradation pathway for ubiquitin fused products (UFD pathway). Several studies have demonstrated that Ufd1 is a component of the Cdc48‐Ufd1‐Npl4 multiprotein complex which is active in the recognition of several polyubiquitin‐tagged proteins and facilitates their presentation to the 26S proteasome for protein degradation or even more specific processing. The multiprotein complex Cdc48‐Ufd‐Npl4 is also active in mammalian cells. The biochemical role of UFD1L protein in human cells is unknown, even though the interaction between UFD1L and NPL4 proteins has been maintained. In order to clarify this issue, we examined the intracellular distribution of the protein in different mammalian cells and studied its involvement in the Fas and ceramide factors‐mediated apoptotic pathways. We established that in mammalian cells, Ufd1l is localized around the nucleus and that it does not interfere with Fas‐and ceramide‐mediated apoptosis. Copyright © 2003 John Wiley & Sons, Ltd. |
| doi_str_mv | 10.1002/cbf.1021 |
| format | Article |
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In yeast, Ufd1 protein is involved in a degradation pathway for ubiquitin fused products (UFD pathway). Several studies have demonstrated that Ufd1 is a component of the Cdc48‐Ufd1‐Npl4 multiprotein complex which is active in the recognition of several polyubiquitin‐tagged proteins and facilitates their presentation to the 26S proteasome for protein degradation or even more specific processing. The multiprotein complex Cdc48‐Ufd‐Npl4 is also active in mammalian cells. The biochemical role of UFD1L protein in human cells is unknown, even though the interaction between UFD1L and NPL4 proteins has been maintained. In order to clarify this issue, we examined the intracellular distribution of the protein in different mammalian cells and studied its involvement in the Fas and ceramide factors‐mediated apoptotic pathways. We established that in mammalian cells, Ufd1l is localized around the nucleus and that it does not interfere with Fas‐and ceramide‐mediated apoptosis. Copyright © 2003 John Wiley & Sons, Ltd.</description><identifier>ISSN: 0263-6484</identifier><identifier>EISSN: 1099-0844</identifier><identifier>DOI: 10.1002/cbf.1021</identifier><identifier>PMID: 12910480</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Animals ; Antibodies, Monoclonal - pharmacology ; apoptosis ; Apoptosis - drug effects ; Apoptosis - physiology ; cellular localization ; Cercopithecus aethiops ; Cloning, Molecular ; COS Cells ; Gene Expression Regulation - genetics ; Genetic Vectors - genetics ; Green Fluorescent Proteins ; Humans ; Inhibitor of Apoptosis Proteins ; Intracellular Space - metabolism ; Luminescent Proteins - genetics ; Luminescent Proteins - metabolism ; Mice ; Microscopy, Fluorescence - methods ; NIH 3T3 Cells ; proteasome ; Proteins - genetics ; Proteins - metabolism ; Proteins - physiology ; Recombinant Fusion Proteins - genetics ; Recombinant Fusion Proteins - metabolism ; Sphingosine - analogs & derivatives ; Sphingosine - pharmacology ; Transfection - methods ; Tumor Cells, Cultured ; ubiquitin ; Viral Proteins - pharmacology</subject><ispartof>Cell biochemistry and function, 2003-09, Vol.21 (3), p.263-267</ispartof><rights>Copyright © 2003 John Wiley & Sons, Ltd.</rights><rights>Copyright 2003 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2701-935ed7d03b9c08c5c52b5477b7dd26b89199ad86c6dddbd007b099606f0c285e3</citedby><cites>FETCH-LOGICAL-c2701-935ed7d03b9c08c5c52b5477b7dd26b89199ad86c6dddbd007b099606f0c285e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fcbf.1021$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fcbf.1021$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12910480$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Amati, Francesca</creatorcontrib><creatorcontrib>Condò, Ivano</creatorcontrib><creatorcontrib>Conti, Emanuela</creatorcontrib><creatorcontrib>Sangiuolo, Federica</creatorcontrib><creatorcontrib>Dallapiccola, Bruno</creatorcontrib><creatorcontrib>Testi, Roberto</creatorcontrib><creatorcontrib>Novelli, Giuseppe</creatorcontrib><title>Analysis of intracellular distribution and apoptosis involvement of the Ufd1l gene product by over-expression studies</title><title>Cell biochemistry and function</title><addtitle>Cell Biochem. Funct</addtitle><description>UFD1L is the human homologue of the yeast ubiquitin fusion degradation 1 (Ufd1) gene and maps on chromosome 22q11.2 in the typically deleted region (TDR) for DiGeorge/velocardiofacial syndromes (DGS/VCFS). In yeast, Ufd1 protein is involved in a degradation pathway for ubiquitin fused products (UFD pathway). Several studies have demonstrated that Ufd1 is a component of the Cdc48‐Ufd1‐Npl4 multiprotein complex which is active in the recognition of several polyubiquitin‐tagged proteins and facilitates their presentation to the 26S proteasome for protein degradation or even more specific processing. The multiprotein complex Cdc48‐Ufd‐Npl4 is also active in mammalian cells. The biochemical role of UFD1L protein in human cells is unknown, even though the interaction between UFD1L and NPL4 proteins has been maintained. In order to clarify this issue, we examined the intracellular distribution of the protein in different mammalian cells and studied its involvement in the Fas and ceramide factors‐mediated apoptotic pathways. We established that in mammalian cells, Ufd1l is localized around the nucleus and that it does not interfere with Fas‐and ceramide‐mediated apoptosis. Copyright © 2003 John Wiley & Sons, Ltd.</description><subject>Animals</subject><subject>Antibodies, Monoclonal - pharmacology</subject><subject>apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Apoptosis - physiology</subject><subject>cellular localization</subject><subject>Cercopithecus aethiops</subject><subject>Cloning, Molecular</subject><subject>COS Cells</subject><subject>Gene Expression Regulation - genetics</subject><subject>Genetic Vectors - genetics</subject><subject>Green Fluorescent Proteins</subject><subject>Humans</subject><subject>Inhibitor of Apoptosis Proteins</subject><subject>Intracellular Space - metabolism</subject><subject>Luminescent Proteins - genetics</subject><subject>Luminescent Proteins - metabolism</subject><subject>Mice</subject><subject>Microscopy, Fluorescence - methods</subject><subject>NIH 3T3 Cells</subject><subject>proteasome</subject><subject>Proteins - genetics</subject><subject>Proteins - metabolism</subject><subject>Proteins - physiology</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>Sphingosine - analogs & derivatives</subject><subject>Sphingosine - pharmacology</subject><subject>Transfection - methods</subject><subject>Tumor Cells, Cultured</subject><subject>ubiquitin</subject><subject>Viral Proteins - pharmacology</subject><issn>0263-6484</issn><issn>1099-0844</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kMtu1DAUQC0EokNB4guQV4hN4DoPO162I_qQKkBoqi4tP27AkImD7QydvyfRRLBidb04Prr3EPKawXsGUH6wppsfJXtCNgykLKCt66dkAyWvCl639Rl5kdIPAJC8gufkjJWSQd3ChkwXg-6PyScaOuqHHLXFvp96HanzKUdvpuzDQPXgqB7DmMPC-uEQ-gPuccjLv_wd6X3nWE-_4YB0jMFNNlNzpOGAscDHMWJKiyblyXlML8mzTvcJX63znNxffdxtb4q7z9e324u7wpYCWCGrBp1wUBlpobWNbUrT1EIY4VzJTSuZlNq13HLnnHEAwszXc-Ad2LJtsDonb0_eeaVfE6as9j4tB-oBw5SUqJqS1zWfwXcn0MaQUsROjdHvdTwqBmpJrObEakk8o29W52T26P6Ba9MZKE7Ab9_j8b8itb28WoUrP_fGx7-8jj8VF5Vo1MOna8V3X2F3-UWqh-oPaCGWMw</recordid><startdate>200309</startdate><enddate>200309</enddate><creator>Amati, Francesca</creator><creator>Condò, Ivano</creator><creator>Conti, Emanuela</creator><creator>Sangiuolo, Federica</creator><creator>Dallapiccola, Bruno</creator><creator>Testi, Roberto</creator><creator>Novelli, Giuseppe</creator><general>John Wiley & Sons, Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200309</creationdate><title>Analysis of intracellular distribution and apoptosis involvement of the Ufd1l gene product by over-expression studies</title><author>Amati, Francesca ; Condò, Ivano ; Conti, Emanuela ; Sangiuolo, Federica ; Dallapiccola, Bruno ; Testi, Roberto ; Novelli, Giuseppe</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2701-935ed7d03b9c08c5c52b5477b7dd26b89199ad86c6dddbd007b099606f0c285e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal - pharmacology</topic><topic>apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Apoptosis - physiology</topic><topic>cellular localization</topic><topic>Cercopithecus aethiops</topic><topic>Cloning, Molecular</topic><topic>COS Cells</topic><topic>Gene Expression Regulation - genetics</topic><topic>Genetic Vectors - genetics</topic><topic>Green Fluorescent Proteins</topic><topic>Humans</topic><topic>Inhibitor of Apoptosis Proteins</topic><topic>Intracellular Space - metabolism</topic><topic>Luminescent Proteins - genetics</topic><topic>Luminescent Proteins - metabolism</topic><topic>Mice</topic><topic>Microscopy, Fluorescence - methods</topic><topic>NIH 3T3 Cells</topic><topic>proteasome</topic><topic>Proteins - genetics</topic><topic>Proteins - metabolism</topic><topic>Proteins - physiology</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Recombinant Fusion Proteins - metabolism</topic><topic>Sphingosine - analogs & derivatives</topic><topic>Sphingosine - pharmacology</topic><topic>Transfection - methods</topic><topic>Tumor Cells, Cultured</topic><topic>ubiquitin</topic><topic>Viral Proteins - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Amati, Francesca</creatorcontrib><creatorcontrib>Condò, Ivano</creatorcontrib><creatorcontrib>Conti, Emanuela</creatorcontrib><creatorcontrib>Sangiuolo, Federica</creatorcontrib><creatorcontrib>Dallapiccola, Bruno</creatorcontrib><creatorcontrib>Testi, Roberto</creatorcontrib><creatorcontrib>Novelli, Giuseppe</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cell biochemistry and function</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Amati, Francesca</au><au>Condò, Ivano</au><au>Conti, Emanuela</au><au>Sangiuolo, Federica</au><au>Dallapiccola, Bruno</au><au>Testi, Roberto</au><au>Novelli, Giuseppe</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of intracellular distribution and apoptosis involvement of the Ufd1l gene product by over-expression studies</atitle><jtitle>Cell biochemistry and function</jtitle><addtitle>Cell Biochem. Funct</addtitle><date>2003-09</date><risdate>2003</risdate><volume>21</volume><issue>3</issue><spage>263</spage><epage>267</epage><pages>263-267</pages><issn>0263-6484</issn><eissn>1099-0844</eissn><abstract>UFD1L is the human homologue of the yeast ubiquitin fusion degradation 1 (Ufd1) gene and maps on chromosome 22q11.2 in the typically deleted region (TDR) for DiGeorge/velocardiofacial syndromes (DGS/VCFS). In yeast, Ufd1 protein is involved in a degradation pathway for ubiquitin fused products (UFD pathway). Several studies have demonstrated that Ufd1 is a component of the Cdc48‐Ufd1‐Npl4 multiprotein complex which is active in the recognition of several polyubiquitin‐tagged proteins and facilitates their presentation to the 26S proteasome for protein degradation or even more specific processing. The multiprotein complex Cdc48‐Ufd‐Npl4 is also active in mammalian cells. The biochemical role of UFD1L protein in human cells is unknown, even though the interaction between UFD1L and NPL4 proteins has been maintained. In order to clarify this issue, we examined the intracellular distribution of the protein in different mammalian cells and studied its involvement in the Fas and ceramide factors‐mediated apoptotic pathways. We established that in mammalian cells, Ufd1l is localized around the nucleus and that it does not interfere with Fas‐and ceramide‐mediated apoptosis. Copyright © 2003 John Wiley & Sons, Ltd.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>12910480</pmid><doi>10.1002/cbf.1021</doi><tpages>5</tpages></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Antibodies, Monoclonal - pharmacology apoptosis Apoptosis - drug effects Apoptosis - physiology cellular localization Cercopithecus aethiops Cloning, Molecular COS Cells Gene Expression Regulation - genetics Genetic Vectors - genetics Green Fluorescent Proteins Humans Inhibitor of Apoptosis Proteins Intracellular Space - metabolism Luminescent Proteins - genetics Luminescent Proteins - metabolism Mice Microscopy, Fluorescence - methods NIH 3T3 Cells proteasome Proteins - genetics Proteins - metabolism Proteins - physiology Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Sphingosine - analogs & derivatives Sphingosine - pharmacology Transfection - methods Tumor Cells, Cultured ubiquitin Viral Proteins - pharmacology |
| title | Analysis of intracellular distribution and apoptosis involvement of the Ufd1l gene product by over-expression studies |
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