Platelet‐Rich Plasma‐Derived Fibrin Clot Formation Stimulates Collagen Synthesis in Periodontal Ligament and Osteoblastic Cells In Vitro
Background: Platelet‐rich plasma (PRP) contains several growth factors, including platelet‐derived growth factor (PDGF) and transforming growth factor‐β (TGF‐β), at high levels. We have demonstrated the PRP functions like TGF‐β to modulate cell proliferation in a cell‐type specific manner. In additi...
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| Veröffentlicht in: | Journal of periodontology (1970) 2003-06, Vol.74 (6), p.858-864 |
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| creator | Kawase, Tomoyuki Okuda, Kazuhiro Wolff, Larry F. Yoshie, Hiromasa |
| description | Background: Platelet‐rich plasma (PRP) contains several growth factors, including platelet‐derived growth factor (PDGF) and transforming growth factor‐β (TGF‐β), at high levels. We have demonstrated the PRP functions like TGF‐β to modulate cell proliferation in a cell‐type specific manner. In addition, PRP forms gel‐like materials in several, but not all, cell cultures tested. This study was designed to investigate PRP's action on extracellular matrix production in periodontal ligament (PDL) and osteoblastic MG63 cell cultures.
Methods: PRP was prepared from the plasma obtained from autologous blood of healthy volunteers and stored at −20°C until used. Cells treated with PRP (0.5% to 2%) were immunocytochemically stained for type I collagen and fibrin and the viscosity of the culture media was visually evaluated. Fibrinogen in PRP was detected by immunodot‐blotting while endogenous thrombin expression in cells was detected by a modified enzymelinked immunosorbent assay.
Results: Gel‐like material rapidly ( |
| doi_str_mv | 10.1902/jop.2003.74.6.858 |
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Methods: PRP was prepared from the plasma obtained from autologous blood of healthy volunteers and stored at −20°C until used. Cells treated with PRP (0.5% to 2%) were immunocytochemically stained for type I collagen and fibrin and the viscosity of the culture media was visually evaluated. Fibrinogen in PRP was detected by immunodot‐blotting while endogenous thrombin expression in cells was detected by a modified enzymelinked immunosorbent assay.
Results: Gel‐like material rapidly (<30 minutes) formed in cultures of either PDL or osteoblastic MG63 cell cultures after addition of PRP (≥0.5%). PRP changed cell shape and up‐regulated type I collagen at 24 hours. Fibrinogen was detected in the PRP preparations and insoluble fibrin networks were found in the newly formed gel‐like material. PRP's action on collagen synthesis was mimicked by purified fibrinogen and blocked by thrombin inhibitor. Thrombin was expressed both in PDL and MG63 cells.
Conclusions: These findings demonstrated that the gel‐like material formed in cell cultures of either PDL or MG63 cells is fibrin clot that is capable of up‐regulating collagen synthesis in the extracellular matrix. Our data suggest the possibility that fibrinogen, converted to fibrin, in combination with growth factors present in PRP might effectively promote wound healing at sites of injury in periodontal tissue. J Periodontol 2003;74:858‐864.</description><identifier>ISSN: 0022-3492</identifier><identifier>EISSN: 1943-3670</identifier><identifier>DOI: 10.1902/jop.2003.74.6.858</identifier><identifier>PMID: 12886997</identifier><language>eng</language><publisher>737 N. Michigan Avenue, Suite 800, Chicago, IL 60611‐2690, USA: American Academy of Periodontology</publisher><subject>Adult ; Blood Platelets - physiology ; Cell Culture Techniques ; Cell Line ; Cell Size - drug effects ; Collagen ; Collagen - biosynthesis ; Collagen - drug effects ; Collagen Type I - biosynthesis ; Collagen Type I - drug effects ; Dentistry ; Extracellular Matrix - drug effects ; Female ; fibrin ; Fibrin - drug effects ; Fibrinogen - analysis ; Humans ; Linear Models ; Male ; osteoblasts ; Osteoblasts - drug effects ; Osteoblasts - metabolism ; Periodontal Ligament - drug effects ; Periodontal Ligament - metabolism ; periodontal ligament/anatomy and histology ; plasma, platelet‐rich ; Platelet-Derived Growth Factor - pharmacology ; thrombin ; Thrombin - analysis ; Thrombin - antagonists & inhibitors ; Transforming Growth Factor beta - pharmacology ; Up-Regulation ; Viscosity</subject><ispartof>Journal of periodontology (1970), 2003-06, Vol.74 (6), p.858-864</ispartof><rights>2003 American Academy of Periodontology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4118-50f5ba383731d6f3003a8341a39d2062090dcb0707186a5300d4ea59c68740823</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1902%2Fjop.2003.74.6.858$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1902%2Fjop.2003.74.6.858$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12886997$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kawase, Tomoyuki</creatorcontrib><creatorcontrib>Okuda, Kazuhiro</creatorcontrib><creatorcontrib>Wolff, Larry F.</creatorcontrib><creatorcontrib>Yoshie, Hiromasa</creatorcontrib><title>Platelet‐Rich Plasma‐Derived Fibrin Clot Formation Stimulates Collagen Synthesis in Periodontal Ligament and Osteoblastic Cells In Vitro</title><title>Journal of periodontology (1970)</title><addtitle>J Periodontol</addtitle><description>Background: Platelet‐rich plasma (PRP) contains several growth factors, including platelet‐derived growth factor (PDGF) and transforming growth factor‐β (TGF‐β), at high levels. We have demonstrated the PRP functions like TGF‐β to modulate cell proliferation in a cell‐type specific manner. In addition, PRP forms gel‐like materials in several, but not all, cell cultures tested. This study was designed to investigate PRP's action on extracellular matrix production in periodontal ligament (PDL) and osteoblastic MG63 cell cultures.
Methods: PRP was prepared from the plasma obtained from autologous blood of healthy volunteers and stored at −20°C until used. Cells treated with PRP (0.5% to 2%) were immunocytochemically stained for type I collagen and fibrin and the viscosity of the culture media was visually evaluated. Fibrinogen in PRP was detected by immunodot‐blotting while endogenous thrombin expression in cells was detected by a modified enzymelinked immunosorbent assay.
Results: Gel‐like material rapidly (<30 minutes) formed in cultures of either PDL or osteoblastic MG63 cell cultures after addition of PRP (≥0.5%). PRP changed cell shape and up‐regulated type I collagen at 24 hours. Fibrinogen was detected in the PRP preparations and insoluble fibrin networks were found in the newly formed gel‐like material. PRP's action on collagen synthesis was mimicked by purified fibrinogen and blocked by thrombin inhibitor. Thrombin was expressed both in PDL and MG63 cells.
Conclusions: These findings demonstrated that the gel‐like material formed in cell cultures of either PDL or MG63 cells is fibrin clot that is capable of up‐regulating collagen synthesis in the extracellular matrix. Our data suggest the possibility that fibrinogen, converted to fibrin, in combination with growth factors present in PRP might effectively promote wound healing at sites of injury in periodontal tissue. J Periodontol 2003;74:858‐864.</description><subject>Adult</subject><subject>Blood Platelets - physiology</subject><subject>Cell Culture Techniques</subject><subject>Cell Line</subject><subject>Cell Size - drug effects</subject><subject>Collagen</subject><subject>Collagen - biosynthesis</subject><subject>Collagen - drug effects</subject><subject>Collagen Type I - biosynthesis</subject><subject>Collagen Type I - drug effects</subject><subject>Dentistry</subject><subject>Extracellular Matrix - drug effects</subject><subject>Female</subject><subject>fibrin</subject><subject>Fibrin - drug effects</subject><subject>Fibrinogen - analysis</subject><subject>Humans</subject><subject>Linear Models</subject><subject>Male</subject><subject>osteoblasts</subject><subject>Osteoblasts - drug effects</subject><subject>Osteoblasts - metabolism</subject><subject>Periodontal Ligament - drug effects</subject><subject>Periodontal Ligament - metabolism</subject><subject>periodontal ligament/anatomy and histology</subject><subject>plasma, platelet‐rich</subject><subject>Platelet-Derived Growth Factor - pharmacology</subject><subject>thrombin</subject><subject>Thrombin - analysis</subject><subject>Thrombin - antagonists & inhibitors</subject><subject>Transforming Growth Factor beta - pharmacology</subject><subject>Up-Regulation</subject><subject>Viscosity</subject><issn>0022-3492</issn><issn>1943-3670</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkb1u2zAUhYmiQeImeYAuBaduUi9_JFJjocZpCgMx8rcSlEQnDCjRJekW3voAGfqMfZLQsIGMnYhDfudc4h6EPhIoSQP0y7NflxSAlYKXdSkr-Q7NSMNZwWoB79EMgNKC8YaeoA8xPmdJOINjdEKolHXTiBl6WTqdjDPp35-_N7Z_wlnHUWf1zQT7ywx4brtgJ9w6n_Dch1En6yd8m-y42Vkjbr1z-tHku-2Unky0EWd-me1-8FPSDi_sox7NlLCeBnwdk_FdnpJsj1vjXMRXE36wKfgzdLTSLprzw3mK7ucXd-33YnF9edV-XRQ9J0QWFayqTjPJBCNDvWJ5A1oyTjRrBgo1hQaGvgMBgshaV_l94EZXTV9LwUFSdoo-73PXwf_cmJjUaGOfv6In4zdRCVZRRgXLINmDffAxBrNS62BHHbaKgNpVoHIFaleBElzVKleQPZ8O4ZtuNMOb47DzDIg98Ns6s_1_ovqxvLiBXfQrvfWWMw</recordid><startdate>200306</startdate><enddate>200306</enddate><creator>Kawase, Tomoyuki</creator><creator>Okuda, Kazuhiro</creator><creator>Wolff, Larry F.</creator><creator>Yoshie, Hiromasa</creator><general>American Academy of Periodontology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200306</creationdate><title>Platelet‐Rich Plasma‐Derived Fibrin Clot Formation Stimulates Collagen Synthesis in Periodontal Ligament and Osteoblastic Cells In Vitro</title><author>Kawase, Tomoyuki ; Okuda, Kazuhiro ; Wolff, Larry F. ; Yoshie, Hiromasa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4118-50f5ba383731d6f3003a8341a39d2062090dcb0707186a5300d4ea59c68740823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Adult</topic><topic>Blood Platelets - physiology</topic><topic>Cell Culture Techniques</topic><topic>Cell Line</topic><topic>Cell Size - drug effects</topic><topic>Collagen</topic><topic>Collagen - biosynthesis</topic><topic>Collagen - drug effects</topic><topic>Collagen Type I - biosynthesis</topic><topic>Collagen Type I - drug effects</topic><topic>Dentistry</topic><topic>Extracellular Matrix - drug effects</topic><topic>Female</topic><topic>fibrin</topic><topic>Fibrin - drug effects</topic><topic>Fibrinogen - analysis</topic><topic>Humans</topic><topic>Linear Models</topic><topic>Male</topic><topic>osteoblasts</topic><topic>Osteoblasts - drug effects</topic><topic>Osteoblasts - metabolism</topic><topic>Periodontal Ligament - drug effects</topic><topic>Periodontal Ligament - metabolism</topic><topic>periodontal ligament/anatomy and histology</topic><topic>plasma, platelet‐rich</topic><topic>Platelet-Derived Growth Factor - pharmacology</topic><topic>thrombin</topic><topic>Thrombin - analysis</topic><topic>Thrombin - antagonists & inhibitors</topic><topic>Transforming Growth Factor beta - pharmacology</topic><topic>Up-Regulation</topic><topic>Viscosity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kawase, Tomoyuki</creatorcontrib><creatorcontrib>Okuda, Kazuhiro</creatorcontrib><creatorcontrib>Wolff, Larry F.</creatorcontrib><creatorcontrib>Yoshie, Hiromasa</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of periodontology (1970)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kawase, Tomoyuki</au><au>Okuda, Kazuhiro</au><au>Wolff, Larry F.</au><au>Yoshie, Hiromasa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Platelet‐Rich Plasma‐Derived Fibrin Clot Formation Stimulates Collagen Synthesis in Periodontal Ligament and Osteoblastic Cells In Vitro</atitle><jtitle>Journal of periodontology (1970)</jtitle><addtitle>J Periodontol</addtitle><date>2003-06</date><risdate>2003</risdate><volume>74</volume><issue>6</issue><spage>858</spage><epage>864</epage><pages>858-864</pages><issn>0022-3492</issn><eissn>1943-3670</eissn><abstract>Background: Platelet‐rich plasma (PRP) contains several growth factors, including platelet‐derived growth factor (PDGF) and transforming growth factor‐β (TGF‐β), at high levels. We have demonstrated the PRP functions like TGF‐β to modulate cell proliferation in a cell‐type specific manner. In addition, PRP forms gel‐like materials in several, but not all, cell cultures tested. This study was designed to investigate PRP's action on extracellular matrix production in periodontal ligament (PDL) and osteoblastic MG63 cell cultures.
Methods: PRP was prepared from the plasma obtained from autologous blood of healthy volunteers and stored at −20°C until used. Cells treated with PRP (0.5% to 2%) were immunocytochemically stained for type I collagen and fibrin and the viscosity of the culture media was visually evaluated. Fibrinogen in PRP was detected by immunodot‐blotting while endogenous thrombin expression in cells was detected by a modified enzymelinked immunosorbent assay.
Results: Gel‐like material rapidly (<30 minutes) formed in cultures of either PDL or osteoblastic MG63 cell cultures after addition of PRP (≥0.5%). PRP changed cell shape and up‐regulated type I collagen at 24 hours. Fibrinogen was detected in the PRP preparations and insoluble fibrin networks were found in the newly formed gel‐like material. PRP's action on collagen synthesis was mimicked by purified fibrinogen and blocked by thrombin inhibitor. Thrombin was expressed both in PDL and MG63 cells.
Conclusions: These findings demonstrated that the gel‐like material formed in cell cultures of either PDL or MG63 cells is fibrin clot that is capable of up‐regulating collagen synthesis in the extracellular matrix. Our data suggest the possibility that fibrinogen, converted to fibrin, in combination with growth factors present in PRP might effectively promote wound healing at sites of injury in periodontal tissue. J Periodontol 2003;74:858‐864.</abstract><cop>737 N. Michigan Avenue, Suite 800, Chicago, IL 60611‐2690, USA</cop><pub>American Academy of Periodontology</pub><pmid>12886997</pmid><doi>10.1902/jop.2003.74.6.858</doi><tpages>7</tpages></addata></record> |
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| subjects | Adult Blood Platelets - physiology Cell Culture Techniques Cell Line Cell Size - drug effects Collagen Collagen - biosynthesis Collagen - drug effects Collagen Type I - biosynthesis Collagen Type I - drug effects Dentistry Extracellular Matrix - drug effects Female fibrin Fibrin - drug effects Fibrinogen - analysis Humans Linear Models Male osteoblasts Osteoblasts - drug effects Osteoblasts - metabolism Periodontal Ligament - drug effects Periodontal Ligament - metabolism periodontal ligament/anatomy and histology plasma, platelet‐rich Platelet-Derived Growth Factor - pharmacology thrombin Thrombin - analysis Thrombin - antagonists & inhibitors Transforming Growth Factor beta - pharmacology Up-Regulation Viscosity |
| title | Platelet‐Rich Plasma‐Derived Fibrin Clot Formation Stimulates Collagen Synthesis in Periodontal Ligament and Osteoblastic Cells In Vitro |
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