Quantitative imaging of protein-protein interactions by multiphoton fluorescence lifetime imaging microscopy using a streak camera

Fluorescence lifetime imaging microscopy (FLIM) using multiphoton excitation techniques is now finding an important place in quantitative imaging of protein-protein interactions and intracellular physiology. Recent developments in multiphoton FLIM methods are reviewed and a novel multiphoton FLIM sy...

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Veröffentlicht in:	Journal of Biomedical Optics 2003-07, Vol.8 (3), p.362-367
Hauptverfasser:	Krishnan, R. V, Masuda, A, Centonze, V. E, Herman, B
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacterial Proteins Caspase 2 Caspases - drug effects Caspases - metabolism Cells, Cultured Cricetinae Equipment Design Equipment Failure Analysis Feasibility Studies fluorescence lifetime imaging microscopy Fluorescence Resonance Energy Transfer - instrumentation Fluorescence Resonance Energy Transfer - methods Green Fluorescent Proteins Kidney - cytology Kidney - drug effects Kidney - enzymology Kidney - metabolism Luminescent Proteins Macromolecular Substances Microscopy, Fluorescence, Multiphoton - instrumentation Microscopy, Fluorescence, Multiphoton - methods Microscopy, Video - instrumentation Microscopy, Video - methods Protein Binding Recombinant Fusion Proteins - metabolism tert-Butylhydroperoxide - pharmacology Time Factors
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