Quantitative imaging of protein-protein interactions by multiphoton fluorescence lifetime imaging microscopy using a streak camera
Fluorescence lifetime imaging microscopy (FLIM) using multiphoton excitation techniques is now finding an important place in quantitative imaging of protein-protein interactions and intracellular physiology. Recent developments in multiphoton FLIM methods are reviewed and a novel multiphoton FLIM sy...
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| Veröffentlicht in: | Journal of Biomedical Optics 2003-07, Vol.8 (3), p.362-367 |
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| container_end_page | 367 |
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| container_issue | 3 |
| container_start_page | 362 |
| container_title | Journal of Biomedical Optics |
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| creator | Krishnan, R. V Masuda, A Centonze, V. E Herman, B |
| description | Fluorescence lifetime imaging microscopy (FLIM) using multiphoton excitation techniques is now finding an important place in quantitative imaging of protein-protein interactions and intracellular physiology. Recent developments in multiphoton FLIM methods are reviewed and a novel multiphoton FLIM system using a streak camera is described. An example of a typical application of the system is provided in which the fluorescence resonance energy transfer between a donor-acceptor pair of fluorescent proteins within a cellular specimen is measured. © |
| doi_str_mv | 10.1117/1.1577574 |
| format | Article |
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V</creatorcontrib><creatorcontrib>Masuda, A</creatorcontrib><creatorcontrib>Centonze, V. E</creatorcontrib><creatorcontrib>Herman, B</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Biomedical Optics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Krishnan, R. V</au><au>Masuda, A</au><au>Centonze, V. E</au><au>Herman, B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantitative imaging of protein-protein interactions by multiphoton fluorescence lifetime imaging microscopy using a streak camera</atitle><jtitle>Journal of Biomedical Optics</jtitle><addtitle>J Biomed Opt</addtitle><date>2003-07-01</date><risdate>2003</risdate><volume>8</volume><issue>3</issue><spage>362</spage><epage>367</epage><pages>362-367</pages><issn>1083-3668</issn><eissn>1560-2281</eissn><coden>JBOPFO</coden><abstract>Fluorescence lifetime imaging microscopy (FLIM) using multiphoton excitation techniques is now finding an important place in quantitative imaging of protein-protein interactions and intracellular physiology. Recent developments in multiphoton FLIM methods are reviewed and a novel multiphoton FLIM system using a streak camera is described. 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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals Bacterial Proteins Caspase 2 Caspases - drug effects Caspases - metabolism Cells, Cultured Cricetinae Equipment Design Equipment Failure Analysis Feasibility Studies fluorescence lifetime imaging microscopy Fluorescence Resonance Energy Transfer - instrumentation Fluorescence Resonance Energy Transfer - methods Green Fluorescent Proteins Kidney - cytology Kidney - drug effects Kidney - enzymology Kidney - metabolism Luminescent Proteins Macromolecular Substances Microscopy, Fluorescence, Multiphoton - instrumentation Microscopy, Fluorescence, Multiphoton - methods Microscopy, Video - instrumentation Microscopy, Video - methods Protein Binding Recombinant Fusion Proteins - metabolism tert-Butylhydroperoxide - pharmacology Time Factors |
| title | Quantitative imaging of protein-protein interactions by multiphoton fluorescence lifetime imaging microscopy using a streak camera |
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