COMPARISON OF A POLYMERASE CHAIN REACTION AND THE KATO-KATZ TECHNIQUE FOR DIAGNOSING INFECTION WITH SCHISTOSOMA MANSONI
Fecal samples from 194 individuals living in an area of Brazil endemic for Schistosoma mansoni were analyzed by a polymerase chain reaction (PCR) and the Kato-Katz parasitologic examination. Statistical analysis of the results showed a kappa index of 0.8 between the two methods. The prevalence of in...
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| Veröffentlicht in: | The American journal of tropical medicine and hygiene 2003-06, Vol.68 (6), p.652-656 |
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| creator | PONTES, LUIS A OLIVEIRA, MARIA C KATZ, NAFTALE DIAS-NETO, EMMANUEL RABELLO, ANA |
| description | Fecal samples from 194 individuals living in an area of Brazil endemic for Schistosoma mansoni were analyzed by a polymerase chain reaction (PCR) and the Kato-Katz parasitologic examination. Statistical analysis of the results showed a kappa index of 0.8 between the two methods. The prevalence of infection was 30.9% in three fecal samples examined by the Kato-Katz method, but 38.1% in one fecal sample examined by the PCR technique. Repeated survey of discordant results showed that five (41.6%) of 12 parasitologically negative cases for which PCR gave positive results were misdiagnosed by Kato-Katz examinations. The PCR technique showed a sensitivity of 96.7% and a specificity of 88% when the parasitologic examination was used as the reference test. The efficacy of cure with praziquantel was 87.8% in three parasitologic stool examinations and 75.6% in one PCR survey. These results demonstrate that the PCR assay might be a valuable alternative for diagnosing Schistosoma infections. |
| doi_str_mv | 10.4269/ajtmh.2003.68.652 |
| format | Article |
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Statistical analysis of the results showed a kappa index of 0.8 between the two methods. The prevalence of infection was 30.9% in three fecal samples examined by the Kato-Katz method, but 38.1% in one fecal sample examined by the PCR technique. Repeated survey of discordant results showed that five (41.6%) of 12 parasitologically negative cases for which PCR gave positive results were misdiagnosed by Kato-Katz examinations. The PCR technique showed a sensitivity of 96.7% and a specificity of 88% when the parasitologic examination was used as the reference test. The efficacy of cure with praziquantel was 87.8% in three parasitologic stool examinations and 75.6% in one PCR survey. 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Statistical analysis of the results showed a kappa index of 0.8 between the two methods. The prevalence of infection was 30.9% in three fecal samples examined by the Kato-Katz method, but 38.1% in one fecal sample examined by the PCR technique. Repeated survey of discordant results showed that five (41.6%) of 12 parasitologically negative cases for which PCR gave positive results were misdiagnosed by Kato-Katz examinations. The PCR technique showed a sensitivity of 96.7% and a specificity of 88% when the parasitologic examination was used as the reference test. The efficacy of cure with praziquantel was 87.8% in three parasitologic stool examinations and 75.6% in one PCR survey. These results demonstrate that the PCR assay might be a valuable alternative for diagnosing Schistosoma infections.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Brazil - epidemiology</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Diseases caused by trematodes</subject><subject>Endemic Diseases</subject><subject>Feces - parasitology</subject><subject>Female</subject><subject>Helminthic diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Parasite Egg Count - methods</subject><subject>Parasitic diseases</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Predictive Value of Tests</subject><subject>Prevalence</subject><subject>Schistosoma mansoni - genetics</subject><subject>Schistosoma mansoni - isolation & purification</subject><subject>Schistosomiases</subject><subject>Schistosomiasis mansoni - diagnosis</subject><subject>Schistosomiasis mansoni - epidemiology</subject><subject>Schistosomiasis mansoni - parasitology</subject><subject>Sensitivity and Specificity</subject><subject>Tropical medicine</subject><issn>0002-9637</issn><issn>1476-1645</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkM1u2zAQhImgReKmfYBeCl6am1xy9UcdCUW2iNpiaikI0gtBS3StQIpT0YbQty8dG_CFS3C_mSEGoa-UTAOIkh_6Zd9vp0CIP43YNArhCk1oEEcejYLwA5oQQsBLIj--QZ-sfSGEMqD0Gt1QYCwmABM0pnL5wFeilAWWM8zxg1w8L7MVLzOc5lwUeJXxtBJuzYt7XOUZ_skr6bnjN66yNC_Er8cMz-QK3ws-L2QpijkWxSw7iZ5EleMyzUVZyVIuOV7ywmWJz-jjRnfWfDnPW_Q4y6o09xZyLlK-8OqAkL2nadzEYWCg9ikYYgwl9SYAphMfWJwkhGoWMwJ0bWoG7tKEEK-bhuq1e4l8_xbdnXzfht3fg7F71be2Nl2nX83uYFXshwAUQgfSE1gPO2sHs1FvQ9vr4Z-iRB3bVu9tq2PbKmLKte00387mh3VvmoviXK8Dvp8BbWvdbQb9Wrf2wgWJ-67PLty2_bMd28Eo2-uuc7ZUjeN4jHsP_A9-aYt7</recordid><startdate>20030601</startdate><enddate>20030601</enddate><creator>PONTES, LUIS A</creator><creator>OLIVEIRA, MARIA C</creator><creator>KATZ, NAFTALE</creator><creator>DIAS-NETO, EMMANUEL</creator><creator>RABELLO, ANA</creator><general>ASTMH</general><general>Allen Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20030601</creationdate><title>COMPARISON OF A POLYMERASE CHAIN REACTION AND THE KATO-KATZ TECHNIQUE FOR DIAGNOSING INFECTION WITH SCHISTOSOMA MANSONI</title><author>PONTES, LUIS A ; OLIVEIRA, MARIA C ; KATZ, NAFTALE ; DIAS-NETO, EMMANUEL ; RABELLO, ANA</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c400t-a17d754e2c312e0ee10cf428a932879901a878021bec82802d527bdd1abbec633</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Brazil - epidemiology</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Diseases caused by trematodes</topic><topic>Endemic Diseases</topic><topic>Feces - parasitology</topic><topic>Female</topic><topic>Helminthic diseases</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Parasite Egg Count - methods</topic><topic>Parasitic diseases</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Predictive Value of Tests</topic><topic>Prevalence</topic><topic>Schistosoma mansoni - genetics</topic><topic>Schistosoma mansoni - isolation & purification</topic><topic>Schistosomiases</topic><topic>Schistosomiasis mansoni - diagnosis</topic><topic>Schistosomiasis mansoni - epidemiology</topic><topic>Schistosomiasis mansoni - parasitology</topic><topic>Sensitivity and Specificity</topic><topic>Tropical medicine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>PONTES, LUIS A</creatorcontrib><creatorcontrib>OLIVEIRA, MARIA C</creatorcontrib><creatorcontrib>KATZ, NAFTALE</creatorcontrib><creatorcontrib>DIAS-NETO, EMMANUEL</creatorcontrib><creatorcontrib>RABELLO, ANA</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The American journal of tropical medicine and hygiene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>PONTES, LUIS A</au><au>OLIVEIRA, MARIA C</au><au>KATZ, NAFTALE</au><au>DIAS-NETO, EMMANUEL</au><au>RABELLO, ANA</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>COMPARISON OF A POLYMERASE CHAIN REACTION AND THE KATO-KATZ TECHNIQUE FOR DIAGNOSING INFECTION WITH SCHISTOSOMA MANSONI</atitle><jtitle>The American journal of tropical medicine and hygiene</jtitle><addtitle>Am J Trop Med Hyg</addtitle><date>2003-06-01</date><risdate>2003</risdate><volume>68</volume><issue>6</issue><spage>652</spage><epage>656</epage><pages>652-656</pages><issn>0002-9637</issn><eissn>1476-1645</eissn><coden>AJTHAB</coden><abstract>Fecal samples from 194 individuals living in an area of Brazil endemic for Schistosoma mansoni were analyzed by a polymerase chain reaction (PCR) and the Kato-Katz parasitologic examination. 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| subjects | Adolescent Adult Aged Animals Biological and medical sciences Brazil - epidemiology Child Child, Preschool Diseases caused by trematodes Endemic Diseases Feces - parasitology Female Helminthic diseases Humans Infectious diseases Male Medical sciences Middle Aged Parasite Egg Count - methods Parasitic diseases Polymerase Chain Reaction - methods Predictive Value of Tests Prevalence Schistosoma mansoni - genetics Schistosoma mansoni - isolation & purification Schistosomiases Schistosomiasis mansoni - diagnosis Schistosomiasis mansoni - epidemiology Schistosomiasis mansoni - parasitology Sensitivity and Specificity Tropical medicine |
| title | COMPARISON OF A POLYMERASE CHAIN REACTION AND THE KATO-KATZ TECHNIQUE FOR DIAGNOSING INFECTION WITH SCHISTOSOMA MANSONI |
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