Detection of ABO blood group polymorphism by denaturing gradient gel electrophoresis
We report the use of a polymerase chain reaction (PCR) format together with denaturing gradient gel electrophoresis (DGGE) which allows rapid identification of the 6 major genotypes (AA, AO, BB, BO, AB and OO) of the human ABO blood group polymorphism in a single amplification. The procedure also di...
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| Veröffentlicht in: | Human molecular genetics 1992-08, Vol.1 (5), p.341-344 |
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| container_title | Human molecular genetics |
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| creator | Johnson, P. H. Hopkinson, D. A. |
| description | We report the use of a polymerase chain reaction (PCR) format together with denaturing gradient gel electrophoresis (DGGE) which allows rapid identification of the 6 major genotypes (AA, AO, BB, BO, AB and OO) of the human ABO blood group polymorphism in a single amplification. The procedure also distinguishes hitherto undescribed polymorphisms associated with the O and B alleles. Thus in testing 95 unrelated European individuals 4 different O alleles, 2 B alleles and 1 A allele were identified by DGGE and the level of recognisable heterozygosity, and hence the information content of the locus as a genetic marker, was raised from 3/95 (3%) to 66/95 (70%). The procedure is robust, genotyping is rapid and clear-cut, and has immediate implications for the use of the ABO locus in linkage analysis on chromosome 9q, the investigation of disease associations and forensic identification. |
| doi_str_mv | 10.1093/hmg/1.5.341 |
| format | Article |
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H. ; Hopkinson, D. A.</creator><creatorcontrib>Johnson, P. H. ; Hopkinson, D. A.</creatorcontrib><description>We report the use of a polymerase chain reaction (PCR) format together with denaturing gradient gel electrophoresis (DGGE) which allows rapid identification of the 6 major genotypes (AA, AO, BB, BO, AB and OO) of the human ABO blood group polymorphism in a single amplification. The procedure also distinguishes hitherto undescribed polymorphisms associated with the O and B alleles. Thus in testing 95 unrelated European individuals 4 different O alleles, 2 B alleles and 1 A allele were identified by DGGE and the level of recognisable heterozygosity, and hence the information content of the locus as a genetic marker, was raised from 3/95 (3%) to 66/95 (70%). The procedure is robust, genotyping is rapid and clear-cut, and has immediate implications for the use of the ABO locus in linkage analysis on chromosome 9q, the investigation of disease associations and forensic identification.</description><identifier>ISSN: 0964-6906</identifier><identifier>EISSN: 1460-2083</identifier><identifier>DOI: 10.1093/hmg/1.5.341</identifier><identifier>PMID: 1303212</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>ABO Blood-Group System - genetics ; ABO system ; Alleles ; Antigens ; Base Sequence ; Biological and medical sciences ; Blood group antigens ; blood groups ; DNA - genetics ; Electrophoresis - methods ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Genotype ; genotyping ; Heterozygote ; Homozygote ; Humans ; man ; Molecular immunology ; Molecular Sequence Data ; Polymerase Chain Reaction ; Polymorphism, Genetic</subject><ispartof>Human molecular genetics, 1992-08, Vol.1 (5), p.341-344</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c363t-15aa3f54fe08b96a61e50cf3cbf29a3632b5b3cdb8245814b49ffee6b88ed3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5621670$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1303212$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Johnson, P. H.</creatorcontrib><creatorcontrib>Hopkinson, D. A.</creatorcontrib><title>Detection of ABO blood group polymorphism by denaturing gradient gel electrophoresis</title><title>Human molecular genetics</title><addtitle>Hum Mol Genet</addtitle><description>We report the use of a polymerase chain reaction (PCR) format together with denaturing gradient gel electrophoresis (DGGE) which allows rapid identification of the 6 major genotypes (AA, AO, BB, BO, AB and OO) of the human ABO blood group polymorphism in a single amplification. The procedure also distinguishes hitherto undescribed polymorphisms associated with the O and B alleles. Thus in testing 95 unrelated European individuals 4 different O alleles, 2 B alleles and 1 A allele were identified by DGGE and the level of recognisable heterozygosity, and hence the information content of the locus as a genetic marker, was raised from 3/95 (3%) to 66/95 (70%). The procedure is robust, genotyping is rapid and clear-cut, and has immediate implications for the use of the ABO locus in linkage analysis on chromosome 9q, the investigation of disease associations and forensic identification.</description><subject>ABO Blood-Group System - genetics</subject><subject>ABO system</subject><subject>Alleles</subject><subject>Antigens</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blood group antigens</subject><subject>blood groups</subject><subject>DNA - genetics</subject><subject>Electrophoresis - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Genotype</subject><subject>genotyping</subject><subject>Heterozygote</subject><subject>Homozygote</subject><subject>Humans</subject><subject>man</subject><subject>Molecular immunology</subject><subject>Molecular Sequence Data</subject><subject>Polymerase Chain Reaction</subject><subject>Polymorphism, Genetic</subject><issn>0964-6906</issn><issn>1460-2083</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0L1v1DAYBnALgcq1MDEjeUAsVa7-TjK2hfZAJ1WCDojFsp3Xd4YkDnYicf89RncqI9M7PD89evUg9IaSNSUtv9oPuyu6lmsu6DO0okKRipGGP0cr0ipRqZaol-g85x-EUCV4fYbOKCecUbZCjx9gBjeHOOLo8fXNA7Z9jB3epbhMeIr9YYhp2oc8YHvAHYxmXlIYdwWYLsA44x30GPrSkeK0jwlyyK_QC2_6DK9P9wJ9ufv4eLuptg_3n26vt5Xjis8VlcZwL4UH0thWGUVBEue5s561phBmpeWusw0TsqHCitZ7AGWbBjp-gd4fS6cUfy2QZz2E7KDvzQhxybrmklFF5X8hVUwRJlSBl0foUsw5gddTCoNJB02J_ru0LktrqqUuSxf99lS72AG6f_Y4bcnfnXKTnel9MqML-YlJVb6rSWHVkYU8w--n2KSfWtW8lnrz7bve3Mu77eftjf7K_wC_kpZv</recordid><startdate>199208</startdate><enddate>199208</enddate><creator>Johnson, P. H.</creator><creator>Hopkinson, D. A.</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T3</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>199208</creationdate><title>Detection of ABO blood group polymorphism by denaturing gradient gel electrophoresis</title><author>Johnson, P. H. ; Hopkinson, D. A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c363t-15aa3f54fe08b96a61e50cf3cbf29a3632b5b3cdb8245814b49ffee6b88ed3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>ABO Blood-Group System - genetics</topic><topic>ABO system</topic><topic>Alleles</topic><topic>Antigens</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blood group antigens</topic><topic>blood groups</topic><topic>DNA - genetics</topic><topic>Electrophoresis - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Genotype</topic><topic>genotyping</topic><topic>Heterozygote</topic><topic>Homozygote</topic><topic>Humans</topic><topic>man</topic><topic>Molecular immunology</topic><topic>Molecular Sequence Data</topic><topic>Polymerase Chain Reaction</topic><topic>Polymorphism, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Johnson, P. H.</creatorcontrib><creatorcontrib>Hopkinson, D. 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Thus in testing 95 unrelated European individuals 4 different O alleles, 2 B alleles and 1 A allele were identified by DGGE and the level of recognisable heterozygosity, and hence the information content of the locus as a genetic marker, was raised from 3/95 (3%) to 66/95 (70%). The procedure is robust, genotyping is rapid and clear-cut, and has immediate implications for the use of the ABO locus in linkage analysis on chromosome 9q, the investigation of disease associations and forensic identification.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>1303212</pmid><doi>10.1093/hmg/1.5.341</doi><tpages>4</tpages></addata></record> |
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| ispartof | Human molecular genetics, 1992-08, Vol.1 (5), p.341-344 |
| issn | 0964-6906 1460-2083 |
| language | eng |
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| source | MEDLINE; Oxford University Press Journals Digital Archive Legacy |
| subjects | ABO Blood-Group System - genetics ABO system Alleles Antigens Base Sequence Biological and medical sciences Blood group antigens blood groups DNA - genetics Electrophoresis - methods Fundamental and applied biological sciences. Psychology Fundamental immunology Genotype genotyping Heterozygote Homozygote Humans man Molecular immunology Molecular Sequence Data Polymerase Chain Reaction Polymorphism, Genetic |
| title | Detection of ABO blood group polymorphism by denaturing gradient gel electrophoresis |
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