Purification and characterization of a milk clotting protease from Mucor bacilliformis

An acid protease having milk clotting activity has been isolated from Mucor bacilliformis cultures. The enzyme was basically purified by ionic exchange chromatography. An average yield of 29 mg purified product was obtained from 100 mL crude extract. As purity criteria, SDS-PAGE, reverse-phase HPLC,...

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Veröffentlicht in:	Applied biochemistry and biotechnology 1992-12, Vol.37 (3), p.283-294
Hauptverfasser:	ARECES, L. B, DE JIMENEZ BONINO, M. B, PARRY, M. A. A, FRAILE, E. R, FERNANDEZ, H. M, CASCONE, O
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acids - analysis Animals Aspartic Acid Endopeptidases - isolation & purification Aspartic Acid Endopeptidases - metabolism Biological and medical sciences Biotechnology Caseins - metabolism Chromatography, High Pressure Liquid Chromatography, Ion Exchange cuajo Culture Media Electrophoresis, Polyacrylamide Gel Enzyme engineering Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration Improved methods for extraction and purification of enzymes Kinetics Methods. Procedures. Technologies microorganisme microorganismos microorganisms Milk - metabolism Molecular Weight mucor Mucor - enzymology presure proteolisis proteolyse proteolysis purificacion purification rennet
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