Pherokine‐2 and ‐3
Drosophila is a powerful model system to study the regulatory and effector mechanisms of innate immunity. To identify molecules induced in the course of viral infection in this insect, we have developed a model based on intrathoracic injection of the picorna‐like Drosophila C virus (DCV). We have us...
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| Veröffentlicht in: | European journal of biochemistry 2003-08, Vol.270 (16), p.3398-3407 |
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| container_title | European journal of biochemistry |
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| creator | Sabatier, Laurence Jouanguy, Emmanuelle Dostert, Catherine Zachary, Daniel Dimarcq, Jean‐Luc Bulet, Philippe Imler, Jean‐Luc |
| description | Drosophila is a powerful model system to study the regulatory and effector mechanisms of innate immunity. To identify molecules induced in the course of viral infection in this insect, we have developed a model based on intrathoracic injection of the picorna‐like Drosophila C virus (DCV). We have used MALDI‐TOF mass spectrometry to compare the hemolymph of DCV infected flies and control flies. By contrast with the strong humoral response triggered by injection of bacteria or fungal spores, we have identified only one molecule induced in the hemolymph of virus infected flies. This molecule, pherokine‐2 (Phk‐2), is related to OS‐D/A10 (Phk‐1), which was previously characterized as a putative odor/pheromone binding protein specifically expressed in antennae. The virus‐induced molecule is also similar to the product of the gene CG9358 (Phk‐3), which is induced by septic injury. Both Phk‐2 and Phk‐3 are strongly expressed during metamorphosis, suggesting that they may participate in tissue‐remodeling. |
| doi_str_mv | 10.1046/j.1432-1033.2003.03725.x |
| format | Article |
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To identify molecules induced in the course of viral infection in this insect, we have developed a model based on intrathoracic injection of the picorna‐like Drosophila C virus (DCV). We have used MALDI‐TOF mass spectrometry to compare the hemolymph of DCV infected flies and control flies. By contrast with the strong humoral response triggered by injection of bacteria or fungal spores, we have identified only one molecule induced in the hemolymph of virus infected flies. This molecule, pherokine‐2 (Phk‐2), is related to OS‐D/A10 (Phk‐1), which was previously characterized as a putative odor/pheromone binding protein specifically expressed in antennae. The virus‐induced molecule is also similar to the product of the gene CG9358 (Phk‐3), which is induced by septic injury. 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To identify molecules induced in the course of viral infection in this insect, we have developed a model based on intrathoracic injection of the picorna‐like Drosophila C virus (DCV). We have used MALDI‐TOF mass spectrometry to compare the hemolymph of DCV infected flies and control flies. By contrast with the strong humoral response triggered by injection of bacteria or fungal spores, we have identified only one molecule induced in the hemolymph of virus infected flies. This molecule, pherokine‐2 (Phk‐2), is related to OS‐D/A10 (Phk‐1), which was previously characterized as a putative odor/pheromone binding protein specifically expressed in antennae. The virus‐induced molecule is also similar to the product of the gene CG9358 (Phk‐3), which is induced by septic injury. Both Phk‐2 and Phk‐3 are strongly expressed during metamorphosis, suggesting that they may participate in tissue‐remodeling.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antibody Formation - immunology</subject><subject>antiviral</subject><subject>Base Sequence</subject><subject>Drosophila - chemistry</subject><subject>Drosophila - immunology</subject><subject>Drosophila - virology</subject><subject>Drosophila C virus</subject><subject>Drosophila Proteins - chemistry</subject><subject>Drosophila Proteins - genetics</subject><subject>Hemolymph - chemistry</subject><subject>Hemolymph - immunology</subject><subject>Hemolymph - virology</subject><subject>host‐defense</subject><subject>Molecular Sequence Data</subject><subject>odor‐binding protein</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><subject>tissue remodelling</subject><issn>0014-2956</issn><issn>1432-1033</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkE1Lw0AQhhdRbKxePUpO3hJn9rN7EbS0KhQU1POySTaYmI-aGNre_An-Rn-Jia16mhfehxnmIcRHCBG4vMhD5IwGCIyFFICFwBQV4XqPeH_FPvEAkAdUCzkiR22bA4DUUh2SEdKJ1lIrj5w-vLimfs0q9_XxSX1bJX4f2DE5SG3RupPdHJPn-expehss7m_upleLIKbIRRBpVFJyPQFMrObohIwRBGVWgbNotbSUaSu0U2mCLE0iLpNU8TimqcWYszE53-5dNvVb59p3U2Zt7IrCVq7uWqOYQN1f6sGzHdhFpUvMsslK22zM7yc9cLkFVlnhNv89mMGYyc0gxgxizGDM_BgzazOfXT8OkX0Dny1dYQ</recordid><startdate>200308</startdate><enddate>200308</enddate><creator>Sabatier, Laurence</creator><creator>Jouanguy, Emmanuelle</creator><creator>Dostert, Catherine</creator><creator>Zachary, Daniel</creator><creator>Dimarcq, Jean‐Luc</creator><creator>Bulet, Philippe</creator><creator>Imler, Jean‐Luc</creator><general>Blackwell Science Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>200308</creationdate><title>Pherokine‐2 and ‐3</title><author>Sabatier, Laurence ; Jouanguy, Emmanuelle ; Dostert, Catherine ; Zachary, Daniel ; Dimarcq, Jean‐Luc ; Bulet, Philippe ; Imler, Jean‐Luc</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2145-b9176649801da941e56c10523a70ea1a96a239a59e7fd13fdb46df74cc2fa1c43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antibody Formation - immunology</topic><topic>antiviral</topic><topic>Base Sequence</topic><topic>Drosophila - chemistry</topic><topic>Drosophila - immunology</topic><topic>Drosophila - virology</topic><topic>Drosophila C virus</topic><topic>Drosophila Proteins - chemistry</topic><topic>Drosophila Proteins - genetics</topic><topic>Hemolymph - chemistry</topic><topic>Hemolymph - immunology</topic><topic>Hemolymph - virology</topic><topic>host‐defense</topic><topic>Molecular Sequence Data</topic><topic>odor‐binding protein</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</topic><topic>tissue remodelling</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sabatier, Laurence</creatorcontrib><creatorcontrib>Jouanguy, Emmanuelle</creatorcontrib><creatorcontrib>Dostert, Catherine</creatorcontrib><creatorcontrib>Zachary, Daniel</creatorcontrib><creatorcontrib>Dimarcq, Jean‐Luc</creatorcontrib><creatorcontrib>Bulet, Philippe</creatorcontrib><creatorcontrib>Imler, Jean‐Luc</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sabatier, Laurence</au><au>Jouanguy, Emmanuelle</au><au>Dostert, Catherine</au><au>Zachary, Daniel</au><au>Dimarcq, Jean‐Luc</au><au>Bulet, Philippe</au><au>Imler, Jean‐Luc</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pherokine‐2 and ‐3</atitle><jtitle>European journal of biochemistry</jtitle><addtitle>Eur J Biochem</addtitle><date>2003-08</date><risdate>2003</risdate><volume>270</volume><issue>16</issue><spage>3398</spage><epage>3407</epage><pages>3398-3407</pages><issn>0014-2956</issn><eissn>1432-1033</eissn><abstract>Drosophila is a powerful model system to study the regulatory and effector mechanisms of innate immunity. To identify molecules induced in the course of viral infection in this insect, we have developed a model based on intrathoracic injection of the picorna‐like Drosophila C virus (DCV). We have used MALDI‐TOF mass spectrometry to compare the hemolymph of DCV infected flies and control flies. By contrast with the strong humoral response triggered by injection of bacteria or fungal spores, we have identified only one molecule induced in the hemolymph of virus infected flies. This molecule, pherokine‐2 (Phk‐2), is related to OS‐D/A10 (Phk‐1), which was previously characterized as a putative odor/pheromone binding protein specifically expressed in antennae. The virus‐induced molecule is also similar to the product of the gene CG9358 (Phk‐3), which is induced by septic injury. 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| subjects | Amino Acid Sequence Animals Antibody Formation - immunology antiviral Base Sequence Drosophila - chemistry Drosophila - immunology Drosophila - virology Drosophila C virus Drosophila Proteins - chemistry Drosophila Proteins - genetics Hemolymph - chemistry Hemolymph - immunology Hemolymph - virology host‐defense Molecular Sequence Data odor‐binding protein Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization tissue remodelling |
| title | Pherokine‐2 and ‐3 |
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