Transient reporter gene (GUS) expression in creeping bentgrass (Agrostis palustris) is affected by in vivo nucleolytic activity
Leaf and callus tissues of a creeping bentgrass cultivar (Penn A4) had high nuclease activities that degraded exogenously added plasmid DNA. When callus tissue was incubated for 24 h with heparin, spermidine, aurintricarboxylic acid or polyethylene glycol, only heparin and spermidine were effective...
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| Veröffentlicht in: | Biotechnology letters 2003-06, Vol.25 (12), p.939-944 |
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| creator | CHHANDAK BASU HONG LUO KAUSCH, Albert P CHANDLEE, Joel M |
| description | Leaf and callus tissues of a creeping bentgrass cultivar (Penn A4) had high nuclease activities that degraded exogenously added plasmid DNA. When callus tissue was incubated for 24 h with heparin, spermidine, aurintricarboxylic acid or polyethylene glycol, only heparin and spermidine were effective as in vitro nuclease inhibitors, protecting exogenously added plasmid DNA from degradation. When beta-glucuronidase (GUS) reporter gene activity was evaluated in heparin-treated (0.6%), 14-month old callus following microprojectile bombardment, GUS activity increased 1000-fold compared to equivalent aged untreated Penn A4 callus. Similar enhancement from heparin pretreatment (0.6% or 1.2%) was not observed in 6-month old callus. This is likely due to much higher activities of nuclease in the younger callus. |
| doi_str_mv | 10.1023/A:1024050720199 |
| format | Article |
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When callus tissue was incubated for 24 h with heparin, spermidine, aurintricarboxylic acid or polyethylene glycol, only heparin and spermidine were effective as in vitro nuclease inhibitors, protecting exogenously added plasmid DNA from degradation. When beta-glucuronidase (GUS) reporter gene activity was evaluated in heparin-treated (0.6%), 14-month old callus following microprojectile bombardment, GUS activity increased 1000-fold compared to equivalent aged untreated Penn A4 callus. Similar enhancement from heparin pretreatment (0.6% or 1.2%) was not observed in 6-month old callus. This is likely due to much higher activities of nuclease in the younger callus.</description><identifier>ISSN: 0141-5492</identifier><identifier>EISSN: 1573-6776</identifier><identifier>DOI: 10.1023/A:1024050720199</identifier><identifier>PMID: 12889827</identifier><identifier>CODEN: BILED3</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>Aging - physiology ; Agrostis - classification ; Agrostis - genetics ; Agrostis - metabolism ; Aurintricarboxylic Acid - pharmacology ; Biological and medical sciences ; Cultivars ; Culture Techniques ; Deoxyribonucleases - antagonists & inhibitors ; Deoxyribonucleases - genetics ; Deoxyribonucleases - metabolism ; Deoxyribonucleic acid ; DNA ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Enzymologic ; Gene Expression Regulation, Plant ; Genes ; Genes, Reporter - genetics ; Glucuronidase - biosynthesis ; Glucuronidase - genetics ; Heparin - pharmacology ; Plant Leaves - genetics ; Plant Leaves - metabolism ; Plants, Genetically Modified - genetics ; Plants, Genetically Modified - metabolism ; Plasmids - administration & dosage ; Polyethylene Glycols - pharmacology ; Species Specificity ; Spermidine - pharmacology ; Transformation, Genetic - genetics ; Transgenes - genetics</subject><ispartof>Biotechnology letters, 2003-06, Vol.25 (12), p.939-944</ispartof><rights>2003 INIST-CNRS</rights><rights>Kluwer Academic Publishers 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14904218$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12889827$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>CHHANDAK BASU</creatorcontrib><creatorcontrib>HONG LUO</creatorcontrib><creatorcontrib>KAUSCH, Albert P</creatorcontrib><creatorcontrib>CHANDLEE, Joel M</creatorcontrib><title>Transient reporter gene (GUS) expression in creeping bentgrass (Agrostis palustris) is affected by in vivo nucleolytic activity</title><title>Biotechnology letters</title><addtitle>Biotechnol Lett</addtitle><description>Leaf and callus tissues of a creeping bentgrass cultivar (Penn A4) had high nuclease activities that degraded exogenously added plasmid DNA. When callus tissue was incubated for 24 h with heparin, spermidine, aurintricarboxylic acid or polyethylene glycol, only heparin and spermidine were effective as in vitro nuclease inhibitors, protecting exogenously added plasmid DNA from degradation. When beta-glucuronidase (GUS) reporter gene activity was evaluated in heparin-treated (0.6%), 14-month old callus following microprojectile bombardment, GUS activity increased 1000-fold compared to equivalent aged untreated Penn A4 callus. Similar enhancement from heparin pretreatment (0.6% or 1.2%) was not observed in 6-month old callus. This is likely due to much higher activities of nuclease in the younger callus.</description><subject>Aging - physiology</subject><subject>Agrostis - classification</subject><subject>Agrostis - genetics</subject><subject>Agrostis - metabolism</subject><subject>Aurintricarboxylic Acid - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Cultivars</subject><subject>Culture Techniques</subject><subject>Deoxyribonucleases - antagonists & inhibitors</subject><subject>Deoxyribonucleases - genetics</subject><subject>Deoxyribonucleases - metabolism</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Fundamental and applied biological sciences. 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Psychology</topic><topic>Gene Expression Regulation, Enzymologic</topic><topic>Gene Expression Regulation, Plant</topic><topic>Genes</topic><topic>Genes, Reporter - genetics</topic><topic>Glucuronidase - biosynthesis</topic><topic>Glucuronidase - genetics</topic><topic>Heparin - pharmacology</topic><topic>Plant Leaves - genetics</topic><topic>Plant Leaves - metabolism</topic><topic>Plants, Genetically Modified - genetics</topic><topic>Plants, Genetically Modified - metabolism</topic><topic>Plasmids - administration & dosage</topic><topic>Polyethylene Glycols - pharmacology</topic><topic>Species Specificity</topic><topic>Spermidine - pharmacology</topic><topic>Transformation, Genetic - genetics</topic><topic>Transgenes - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>CHHANDAK BASU</creatorcontrib><creatorcontrib>HONG LUO</creatorcontrib><creatorcontrib>KAUSCH, Albert P</creatorcontrib><creatorcontrib>CHANDLEE, Joel M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Engineering Collection</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Biotechnology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>CHHANDAK BASU</au><au>HONG LUO</au><au>KAUSCH, Albert P</au><au>CHANDLEE, Joel M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transient reporter gene (GUS) expression in creeping bentgrass (Agrostis palustris) is affected by in vivo nucleolytic activity</atitle><jtitle>Biotechnology letters</jtitle><addtitle>Biotechnol Lett</addtitle><date>2003-06-01</date><risdate>2003</risdate><volume>25</volume><issue>12</issue><spage>939</spage><epage>944</epage><pages>939-944</pages><issn>0141-5492</issn><eissn>1573-6776</eissn><coden>BILED3</coden><abstract>Leaf and callus tissues of a creeping bentgrass cultivar (Penn A4) had high nuclease activities that degraded exogenously added plasmid DNA. When callus tissue was incubated for 24 h with heparin, spermidine, aurintricarboxylic acid or polyethylene glycol, only heparin and spermidine were effective as in vitro nuclease inhibitors, protecting exogenously added plasmid DNA from degradation. When beta-glucuronidase (GUS) reporter gene activity was evaluated in heparin-treated (0.6%), 14-month old callus following microprojectile bombardment, GUS activity increased 1000-fold compared to equivalent aged untreated Penn A4 callus. Similar enhancement from heparin pretreatment (0.6% or 1.2%) was not observed in 6-month old callus. This is likely due to much higher activities of nuclease in the younger callus.</abstract><cop>Dordrecht</cop><pub>Springer</pub><pmid>12889827</pmid><doi>10.1023/A:1024050720199</doi><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 0141-5492 |
| ispartof | Biotechnology letters, 2003-06, Vol.25 (12), p.939-944 |
| issn | 0141-5492 1573-6776 |
| language | eng |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Aging - physiology Agrostis - classification Agrostis - genetics Agrostis - metabolism Aurintricarboxylic Acid - pharmacology Biological and medical sciences Cultivars Culture Techniques Deoxyribonucleases - antagonists & inhibitors Deoxyribonucleases - genetics Deoxyribonucleases - metabolism Deoxyribonucleic acid DNA Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Genes Genes, Reporter - genetics Glucuronidase - biosynthesis Glucuronidase - genetics Heparin - pharmacology Plant Leaves - genetics Plant Leaves - metabolism Plants, Genetically Modified - genetics Plants, Genetically Modified - metabolism Plasmids - administration & dosage Polyethylene Glycols - pharmacology Species Specificity Spermidine - pharmacology Transformation, Genetic - genetics Transgenes - genetics |
| title | Transient reporter gene (GUS) expression in creeping bentgrass (Agrostis palustris) is affected by in vivo nucleolytic activity |
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