Targeted breakage of a human chromosome mediated by cloned human telomeric DNA
Novel approaches to the structural and functional analysis of mammalian chromosomes would be possible if the gross structure of the chromosomes in living cells could be engineered. Controlled modifications can be engineered by conventional targeting techniques based on homologous recombination. Larg...
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| Veröffentlicht in: | Nature genetics 1992-12, Vol.2 (4), p.283-287 |
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| container_title | Nature genetics |
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| creator | Itzhaki, J.E Barnett, M.A MacCarthy, A.B Buckle, V.J Brown, W.R.A Porter, A.C.G |
| description | Novel approaches to the structural and functional analysis of mammalian chromosomes would be possible if the gross structure of the chromosomes in living cells could be engineered. Controlled modifications can be engineered by conventional targeting techniques based on homologous recombination. Large but uncontrolled modifications can be made by the integration of cloned human telomeric DNA. We describe here the combined use of gene targeting and telomere–mediated chromosome breakage to generate a defined truncation of a human chromosome. Telomeric DNA was targeted to the 6–16 gene on the short arm of chromosome 1 in a human cell line. Molecular and cytogenetic analyses showed that, of eight targeted clones that were isolated, one clone had the predicted truncation of chromosome 1. |
| doi_str_mv | 10.1038/ng1292-283 |
| format | Article |
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Controlled modifications can be engineered by conventional targeting techniques based on homologous recombination. Large but uncontrolled modifications can be made by the integration of cloned human telomeric DNA. We describe here the combined use of gene targeting and telomere–mediated chromosome breakage to generate a defined truncation of a human chromosome. Telomeric DNA was targeted to the 6–16 gene on the short arm of chromosome 1 in a human cell line. 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Controlled modifications can be engineered by conventional targeting techniques based on homologous recombination. Large but uncontrolled modifications can be made by the integration of cloned human telomeric DNA. We describe here the combined use of gene targeting and telomere–mediated chromosome breakage to generate a defined truncation of a human chromosome. Telomeric DNA was targeted to the 6–16 gene on the short arm of chromosome 1 in a human cell line. Molecular and cytogenetic analyses showed that, of eight targeted clones that were isolated, one clone had the predicted truncation of chromosome 1.</description><subject>Agriculture</subject><subject>Animal Genetics and Genomics</subject><subject>biochemical techniques</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>breaks</subject><subject>Cancer Research</subject><subject>Cell Line</subject><subject>chromosome 1</subject><subject>chromosome breakage</subject><subject>Chromosome Deletion</subject><subject>chromosomes</subject><subject>Chromosomes, Human, Pair 1 - ultrastructure</subject><subject>cloning</subject><subject>Cloning, Molecular</subject><subject>cytogenetic analysis</subject><subject>cytogenetics</subject><subject>DNA</subject><subject>DNA - genetics</subject><subject>Gene Function</subject><subject>gene targeting</subject><subject>Genetic Engineering</subject><subject>Genetic Techniques</subject><subject>homologous recombination</subject><subject>Human Genetics</subject><subject>Humans</subject><subject>man</subject><subject>news-and-views</subject><subject>Recombination, Genetic</subject><subject>targeted chromosome breakage</subject><subject>Telomere - ultrastructure</subject><subject>telomeres</subject><subject>truncation</subject><issn>1061-4036</issn><issn>1546-1718</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkb9v1zAQxS1U1F-wsCM8dQAF7vwjccavWgpIVRloZ8txLmlKErd2MvS_x5CKLpVgupPeR-9O7zH2BuEjgjSf5h5FLQph5At2iFqVBVZo9vIOJRYKZHnAjlK6BUClwOyzfZQghYFDdnnlYk8LtbyJ5H66nnjouOM36-Rm7m9imEIKE_GJ2sH94R64H8Oct41ZaMx6HDw_u9y9Yi87NyZ6_TiP2fX556vTr8XF9y_fTncXhVe1XoquFUgtyKpV6CrTyIYMOKS61obyi6hBevKCuko3WYW6I1TgTCe1UMrLY3ay-d7FcL9SWuw0JE_j6GYKa7KV1CjKWv0TxLKUxhj4D1ChEVJn8P0G-hhSitTZuzhMLj5YBPu7DrvVYXMdGX776Lo2OcEndMs_6x82PWVl7ina27DGOUf3vBvf6Nkta6S_bk_9Z-TdhnQuWNfHIdnrHwLyPazKnJ2WvwAeIKW1</recordid><startdate>19921201</startdate><enddate>19921201</enddate><creator>Itzhaki, J.E</creator><creator>Barnett, M.A</creator><creator>MacCarthy, A.B</creator><creator>Buckle, V.J</creator><creator>Brown, W.R.A</creator><creator>Porter, A.C.G</creator><general>Nature Publishing Group US</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T3</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19921201</creationdate><title>Targeted breakage of a human chromosome mediated by cloned human telomeric DNA</title><author>Itzhaki, J.E ; Barnett, M.A ; MacCarthy, A.B ; Buckle, V.J ; Brown, W.R.A ; Porter, A.C.G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c495t-fd21ed037d41a78b3be80a1e9958e4401503cec2ef75bb3b09fe140a8f35244c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Agriculture</topic><topic>Animal Genetics and Genomics</topic><topic>biochemical techniques</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>breaks</topic><topic>Cancer Research</topic><topic>Cell Line</topic><topic>chromosome 1</topic><topic>chromosome breakage</topic><topic>Chromosome Deletion</topic><topic>chromosomes</topic><topic>Chromosomes, Human, Pair 1 - ultrastructure</topic><topic>cloning</topic><topic>Cloning, Molecular</topic><topic>cytogenetic analysis</topic><topic>cytogenetics</topic><topic>DNA</topic><topic>DNA - genetics</topic><topic>Gene Function</topic><topic>gene targeting</topic><topic>Genetic Engineering</topic><topic>Genetic Techniques</topic><topic>homologous recombination</topic><topic>Human Genetics</topic><topic>Humans</topic><topic>man</topic><topic>news-and-views</topic><topic>Recombination, Genetic</topic><topic>targeted chromosome breakage</topic><topic>Telomere - ultrastructure</topic><topic>telomeres</topic><topic>truncation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Itzhaki, J.E</creatorcontrib><creatorcontrib>Barnett, M.A</creatorcontrib><creatorcontrib>MacCarthy, A.B</creatorcontrib><creatorcontrib>Buckle, V.J</creatorcontrib><creatorcontrib>Brown, W.R.A</creatorcontrib><creatorcontrib>Porter, A.C.G</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Human Genome Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Nature genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Itzhaki, J.E</au><au>Barnett, M.A</au><au>MacCarthy, A.B</au><au>Buckle, V.J</au><au>Brown, W.R.A</au><au>Porter, A.C.G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Targeted breakage of a human chromosome mediated by cloned human telomeric DNA</atitle><jtitle>Nature genetics</jtitle><stitle>Nat Genet</stitle><addtitle>Nat Genet</addtitle><date>1992-12-01</date><risdate>1992</risdate><volume>2</volume><issue>4</issue><spage>283</spage><epage>287</epage><pages>283-287</pages><issn>1061-4036</issn><eissn>1546-1718</eissn><abstract>Novel approaches to the structural and functional analysis of mammalian chromosomes would be possible if the gross structure of the chromosomes in living cells could be engineered. Controlled modifications can be engineered by conventional targeting techniques based on homologous recombination. Large but uncontrolled modifications can be made by the integration of cloned human telomeric DNA. We describe here the combined use of gene targeting and telomere–mediated chromosome breakage to generate a defined truncation of a human chromosome. Telomeric DNA was targeted to the 6–16 gene on the short arm of chromosome 1 in a human cell line. Molecular and cytogenetic analyses showed that, of eight targeted clones that were isolated, one clone had the predicted truncation of chromosome 1.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><pmid>1303280</pmid><doi>10.1038/ng1292-283</doi><tpages>5</tpages></addata></record> |
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| source | MEDLINE; Nature Journals Online; SpringerLink Journals - AutoHoldings |
| subjects | Agriculture Animal Genetics and Genomics biochemical techniques Biomedical and Life Sciences Biomedicine breaks Cancer Research Cell Line chromosome 1 chromosome breakage Chromosome Deletion chromosomes Chromosomes, Human, Pair 1 - ultrastructure cloning Cloning, Molecular cytogenetic analysis cytogenetics DNA DNA - genetics Gene Function gene targeting Genetic Engineering Genetic Techniques homologous recombination Human Genetics Humans man news-and-views Recombination, Genetic targeted chromosome breakage Telomere - ultrastructure telomeres truncation |
| title | Targeted breakage of a human chromosome mediated by cloned human telomeric DNA |
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