Up-Regulation of Urokinase-Type Plasminogen Activator in Corneal Epithelial Cells Induced by Wounding
To investigate the possible role of urokinase-type plasminogen activator (uPA) in corneal epithelial wound healing by examining its expression both in the rabbit corneal epithelium in situ and in rabbit corneal epithelial (RCE) cells in vitro. The rabbit cornea was subjected to mechanical wounding,...
Gespeichert in:
| Veröffentlicht in: | Investigative ophthalmology & visual science 2003-08, Vol.44 (8), p.3332-3338 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 3338 |
|---|---|
| container_issue | 8 |
| container_start_page | 3332 |
| container_title | Investigative ophthalmology & visual science |
| container_volume | 44 |
| creator | Watanabe, Masanao Yano, Wataru Kondo, Shoichi Hattori, Yukio Yamada, Naoyuki Yanai, Ryoji Nishida, Teruo |
| description | To investigate the possible role of urokinase-type plasminogen activator (uPA) in corneal epithelial wound healing by examining its expression both in the rabbit corneal epithelium in situ and in rabbit corneal epithelial (RCE) cells in vitro.
The rabbit cornea was subjected to mechanical wounding, and frozen sections of the tissue were subsequently prepared and subjected both to immunostaining with antibodies to uPA and to in situ zymography for the detection of PA activity. RCE cell monolayers were also subjected to scrape wounding, after which they were immunostained for uPA. The amounts of uPA protein in the culture medium and of uPA mRNA in cell lysates were also determined by enzyme-linked immunosorbent assay and by reverse transcription and real-time quantitative polymerase chain reaction analysis, respectively.
Immunostaining and in situ zymography of the wounded cornea revealed that uPA was restricted to the leading edge of the migrating corneal epithelium. In contrast, tissue-type PA was expressed throughout the corneal epithelium. Scraping of RCE cell monolayers induced the expression of uPA in the migrating cells at the wound edge. The amount of uPA in the culture medium of RCE cells increased with the number of scrape wounds applied. Wounding also induced a time-dependent increase in the abundance of uPA mRNA in the cell monolayers. The migration of RCE cells was inhibited by antibodies to uPA.
Mechanical wounding induces up-regulation of uPA at both the protein and mRNA levels in corneal epithelial cells. uPA may thus contribute to epithelial cell migration during corneal epithelial wound healing. |
| doi_str_mv | 10.1167/iovs.02-1280 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73510327</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>73510327</sourcerecordid><originalsourceid>FETCH-LOGICAL-c452t-6483697ec452fa040449a908094cbfd0c1bac926201f2f47806990745db097693</originalsourceid><addsrcrecordid>eNpFkM1r2zAYh0XZWLN2t56HLutpbl992LKOJbRbobAxGnYUsiwnWmXJleyG_PezaSAnvYKHHw8PQlcEbgipxK2Lb_kGaEFoDWdoRcqSFqWo2Qe0AsKrAjjwc_Q5538AlBAKn9D5zNZUiHqF7GYo_tjt5PXoYsCxw5sUX1zQ2RbPh8Hi317n3oW4tQHfmdG96TEm7AJexxSs9vh-cOPOejefa-t9xo-hnYxtcXPAf-MUWhe2l-hjp322X47vBdo83D-vfxZPv348ru-eCsNLOhYVr1klhV1-nV7EudQSapDcNF0LhjTaSFpRIB3tuKihkhIEL9sGpKgku0DX77tDiq-TzaPqXTazlQ42TlkJVhJgVMzg93fQpJhzsp0akut1OigCasmqlqwKqFqyzvjX4-7U9LY9wceOM_DtCOhstO-SDsblE1fC7Cyrk-DObXd7l6zKvfZ-niVqv99zrmrFGKPsPxaNjQg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>73510327</pqid></control><display><type>article</type><title>Up-Regulation of Urokinase-Type Plasminogen Activator in Corneal Epithelial Cells Induced by Wounding</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Watanabe, Masanao ; Yano, Wataru ; Kondo, Shoichi ; Hattori, Yukio ; Yamada, Naoyuki ; Yanai, Ryoji ; Nishida, Teruo</creator><creatorcontrib>Watanabe, Masanao ; Yano, Wataru ; Kondo, Shoichi ; Hattori, Yukio ; Yamada, Naoyuki ; Yanai, Ryoji ; Nishida, Teruo</creatorcontrib><description>To investigate the possible role of urokinase-type plasminogen activator (uPA) in corneal epithelial wound healing by examining its expression both in the rabbit corneal epithelium in situ and in rabbit corneal epithelial (RCE) cells in vitro.
The rabbit cornea was subjected to mechanical wounding, and frozen sections of the tissue were subsequently prepared and subjected both to immunostaining with antibodies to uPA and to in situ zymography for the detection of PA activity. RCE cell monolayers were also subjected to scrape wounding, after which they were immunostained for uPA. The amounts of uPA protein in the culture medium and of uPA mRNA in cell lysates were also determined by enzyme-linked immunosorbent assay and by reverse transcription and real-time quantitative polymerase chain reaction analysis, respectively.
Immunostaining and in situ zymography of the wounded cornea revealed that uPA was restricted to the leading edge of the migrating corneal epithelium. In contrast, tissue-type PA was expressed throughout the corneal epithelium. Scraping of RCE cell monolayers induced the expression of uPA in the migrating cells at the wound edge. The amount of uPA in the culture medium of RCE cells increased with the number of scrape wounds applied. Wounding also induced a time-dependent increase in the abundance of uPA mRNA in the cell monolayers. The migration of RCE cells was inhibited by antibodies to uPA.
Mechanical wounding induces up-regulation of uPA at both the protein and mRNA levels in corneal epithelial cells. uPA may thus contribute to epithelial cell migration during corneal epithelial wound healing.</description><identifier>ISSN: 0146-0404</identifier><identifier>ISSN: 1552-5783</identifier><identifier>EISSN: 1552-5783</identifier><identifier>DOI: 10.1167/iovs.02-1280</identifier><identifier>PMID: 12882778</identifier><identifier>CODEN: IOVSDA</identifier><language>eng</language><publisher>Rockville, MD: ARVO</publisher><subject>Animals ; Antibodies, Monoclonal ; Biological and medical sciences ; Cell Movement ; Cells, Cultured ; Enzyme-Linked Immunosorbent Assay ; Epithelial Cells - enzymology ; Epithelium, Corneal - enzymology ; Epithelium, Corneal - injuries ; Epithelium, Corneal - pathology ; Eye ; Eye Injuries, Penetrating - enzymology ; Eye Injuries, Penetrating - pathology ; Fluorescent Antibody Technique, Indirect ; Gene Expression Regulation, Enzymologic ; Male ; Medical sciences ; Microscopy, Fluorescence ; Pharmacology. Drug treatments ; Rabbits ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - metabolism ; Up-Regulation ; Urokinase-Type Plasminogen Activator - genetics ; Urokinase-Type Plasminogen Activator - metabolism ; Wound Healing</subject><ispartof>Investigative ophthalmology & visual science, 2003-08, Vol.44 (8), p.3332-3338</ispartof><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-6483697ec452fa040449a908094cbfd0c1bac926201f2f47806990745db097693</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15020196$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12882778$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Watanabe, Masanao</creatorcontrib><creatorcontrib>Yano, Wataru</creatorcontrib><creatorcontrib>Kondo, Shoichi</creatorcontrib><creatorcontrib>Hattori, Yukio</creatorcontrib><creatorcontrib>Yamada, Naoyuki</creatorcontrib><creatorcontrib>Yanai, Ryoji</creatorcontrib><creatorcontrib>Nishida, Teruo</creatorcontrib><title>Up-Regulation of Urokinase-Type Plasminogen Activator in Corneal Epithelial Cells Induced by Wounding</title><title>Investigative ophthalmology & visual science</title><addtitle>Invest Ophthalmol Vis Sci</addtitle><description>To investigate the possible role of urokinase-type plasminogen activator (uPA) in corneal epithelial wound healing by examining its expression both in the rabbit corneal epithelium in situ and in rabbit corneal epithelial (RCE) cells in vitro.
The rabbit cornea was subjected to mechanical wounding, and frozen sections of the tissue were subsequently prepared and subjected both to immunostaining with antibodies to uPA and to in situ zymography for the detection of PA activity. RCE cell monolayers were also subjected to scrape wounding, after which they were immunostained for uPA. The amounts of uPA protein in the culture medium and of uPA mRNA in cell lysates were also determined by enzyme-linked immunosorbent assay and by reverse transcription and real-time quantitative polymerase chain reaction analysis, respectively.
Immunostaining and in situ zymography of the wounded cornea revealed that uPA was restricted to the leading edge of the migrating corneal epithelium. In contrast, tissue-type PA was expressed throughout the corneal epithelium. Scraping of RCE cell monolayers induced the expression of uPA in the migrating cells at the wound edge. The amount of uPA in the culture medium of RCE cells increased with the number of scrape wounds applied. Wounding also induced a time-dependent increase in the abundance of uPA mRNA in the cell monolayers. The migration of RCE cells was inhibited by antibodies to uPA.
Mechanical wounding induces up-regulation of uPA at both the protein and mRNA levels in corneal epithelial cells. uPA may thus contribute to epithelial cell migration during corneal epithelial wound healing.</description><subject>Animals</subject><subject>Antibodies, Monoclonal</subject><subject>Biological and medical sciences</subject><subject>Cell Movement</subject><subject>Cells, Cultured</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Epithelial Cells - enzymology</subject><subject>Epithelium, Corneal - enzymology</subject><subject>Epithelium, Corneal - injuries</subject><subject>Epithelium, Corneal - pathology</subject><subject>Eye</subject><subject>Eye Injuries, Penetrating - enzymology</subject><subject>Eye Injuries, Penetrating - pathology</subject><subject>Fluorescent Antibody Technique, Indirect</subject><subject>Gene Expression Regulation, Enzymologic</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Microscopy, Fluorescence</subject><subject>Pharmacology. Drug treatments</subject><subject>Rabbits</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - metabolism</subject><subject>Up-Regulation</subject><subject>Urokinase-Type Plasminogen Activator - genetics</subject><subject>Urokinase-Type Plasminogen Activator - metabolism</subject><subject>Wound Healing</subject><issn>0146-0404</issn><issn>1552-5783</issn><issn>1552-5783</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkM1r2zAYh0XZWLN2t56HLutpbl992LKOJbRbobAxGnYUsiwnWmXJleyG_PezaSAnvYKHHw8PQlcEbgipxK2Lb_kGaEFoDWdoRcqSFqWo2Qe0AsKrAjjwc_Q5538AlBAKn9D5zNZUiHqF7GYo_tjt5PXoYsCxw5sUX1zQ2RbPh8Hi317n3oW4tQHfmdG96TEm7AJexxSs9vh-cOPOejefa-t9xo-hnYxtcXPAf-MUWhe2l-hjp322X47vBdo83D-vfxZPv348ru-eCsNLOhYVr1klhV1-nV7EudQSapDcNF0LhjTaSFpRIB3tuKihkhIEL9sGpKgku0DX77tDiq-TzaPqXTazlQ42TlkJVhJgVMzg93fQpJhzsp0akut1OigCasmqlqwKqFqyzvjX4-7U9LY9wceOM_DtCOhstO-SDsblE1fC7Cyrk-DObXd7l6zKvfZ-niVqv99zrmrFGKPsPxaNjQg</recordid><startdate>20030801</startdate><enddate>20030801</enddate><creator>Watanabe, Masanao</creator><creator>Yano, Wataru</creator><creator>Kondo, Shoichi</creator><creator>Hattori, Yukio</creator><creator>Yamada, Naoyuki</creator><creator>Yanai, Ryoji</creator><creator>Nishida, Teruo</creator><general>ARVO</general><general>Association for Research in Vision and Ophtalmology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20030801</creationdate><title>Up-Regulation of Urokinase-Type Plasminogen Activator in Corneal Epithelial Cells Induced by Wounding</title><author>Watanabe, Masanao ; Yano, Wataru ; Kondo, Shoichi ; Hattori, Yukio ; Yamada, Naoyuki ; Yanai, Ryoji ; Nishida, Teruo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-6483697ec452fa040449a908094cbfd0c1bac926201f2f47806990745db097693</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal</topic><topic>Biological and medical sciences</topic><topic>Cell Movement</topic><topic>Cells, Cultured</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Epithelial Cells - enzymology</topic><topic>Epithelium, Corneal - enzymology</topic><topic>Epithelium, Corneal - injuries</topic><topic>Epithelium, Corneal - pathology</topic><topic>Eye</topic><topic>Eye Injuries, Penetrating - enzymology</topic><topic>Eye Injuries, Penetrating - pathology</topic><topic>Fluorescent Antibody Technique, Indirect</topic><topic>Gene Expression Regulation, Enzymologic</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Microscopy, Fluorescence</topic><topic>Pharmacology. Drug treatments</topic><topic>Rabbits</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - metabolism</topic><topic>Up-Regulation</topic><topic>Urokinase-Type Plasminogen Activator - genetics</topic><topic>Urokinase-Type Plasminogen Activator - metabolism</topic><topic>Wound Healing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Watanabe, Masanao</creatorcontrib><creatorcontrib>Yano, Wataru</creatorcontrib><creatorcontrib>Kondo, Shoichi</creatorcontrib><creatorcontrib>Hattori, Yukio</creatorcontrib><creatorcontrib>Yamada, Naoyuki</creatorcontrib><creatorcontrib>Yanai, Ryoji</creatorcontrib><creatorcontrib>Nishida, Teruo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Investigative ophthalmology & visual science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Watanabe, Masanao</au><au>Yano, Wataru</au><au>Kondo, Shoichi</au><au>Hattori, Yukio</au><au>Yamada, Naoyuki</au><au>Yanai, Ryoji</au><au>Nishida, Teruo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Up-Regulation of Urokinase-Type Plasminogen Activator in Corneal Epithelial Cells Induced by Wounding</atitle><jtitle>Investigative ophthalmology & visual science</jtitle><addtitle>Invest Ophthalmol Vis Sci</addtitle><date>2003-08-01</date><risdate>2003</risdate><volume>44</volume><issue>8</issue><spage>3332</spage><epage>3338</epage><pages>3332-3338</pages><issn>0146-0404</issn><issn>1552-5783</issn><eissn>1552-5783</eissn><coden>IOVSDA</coden><abstract>To investigate the possible role of urokinase-type plasminogen activator (uPA) in corneal epithelial wound healing by examining its expression both in the rabbit corneal epithelium in situ and in rabbit corneal epithelial (RCE) cells in vitro.
The rabbit cornea was subjected to mechanical wounding, and frozen sections of the tissue were subsequently prepared and subjected both to immunostaining with antibodies to uPA and to in situ zymography for the detection of PA activity. RCE cell monolayers were also subjected to scrape wounding, after which they were immunostained for uPA. The amounts of uPA protein in the culture medium and of uPA mRNA in cell lysates were also determined by enzyme-linked immunosorbent assay and by reverse transcription and real-time quantitative polymerase chain reaction analysis, respectively.
Immunostaining and in situ zymography of the wounded cornea revealed that uPA was restricted to the leading edge of the migrating corneal epithelium. In contrast, tissue-type PA was expressed throughout the corneal epithelium. Scraping of RCE cell monolayers induced the expression of uPA in the migrating cells at the wound edge. The amount of uPA in the culture medium of RCE cells increased with the number of scrape wounds applied. Wounding also induced a time-dependent increase in the abundance of uPA mRNA in the cell monolayers. The migration of RCE cells was inhibited by antibodies to uPA.
Mechanical wounding induces up-regulation of uPA at both the protein and mRNA levels in corneal epithelial cells. uPA may thus contribute to epithelial cell migration during corneal epithelial wound healing.</abstract><cop>Rockville, MD</cop><pub>ARVO</pub><pmid>12882778</pmid><doi>10.1167/iovs.02-1280</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0146-0404 |
| ispartof | Investigative ophthalmology & visual science, 2003-08, Vol.44 (8), p.3332-3338 |
| issn | 0146-0404 1552-5783 1552-5783 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73510327 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals Antibodies, Monoclonal Biological and medical sciences Cell Movement Cells, Cultured Enzyme-Linked Immunosorbent Assay Epithelial Cells - enzymology Epithelium, Corneal - enzymology Epithelium, Corneal - injuries Epithelium, Corneal - pathology Eye Eye Injuries, Penetrating - enzymology Eye Injuries, Penetrating - pathology Fluorescent Antibody Technique, Indirect Gene Expression Regulation, Enzymologic Male Medical sciences Microscopy, Fluorescence Pharmacology. Drug treatments Rabbits Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism Up-Regulation Urokinase-Type Plasminogen Activator - genetics Urokinase-Type Plasminogen Activator - metabolism Wound Healing |
| title | Up-Regulation of Urokinase-Type Plasminogen Activator in Corneal Epithelial Cells Induced by Wounding |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T03%3A53%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Up-Regulation%20of%20Urokinase-Type%20Plasminogen%20Activator%20in%20Corneal%20Epithelial%20Cells%20Induced%20by%20Wounding&rft.jtitle=Investigative%20ophthalmology%20&%20visual%20science&rft.au=Watanabe,%20Masanao&rft.date=2003-08-01&rft.volume=44&rft.issue=8&rft.spage=3332&rft.epage=3338&rft.pages=3332-3338&rft.issn=0146-0404&rft.eissn=1552-5783&rft.coden=IOVSDA&rft_id=info:doi/10.1167/iovs.02-1280&rft_dat=%3Cproquest_cross%3E73510327%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=73510327&rft_id=info:pmid/12882778&rfr_iscdi=true |