Apoptosis and In Vitro Development of Preimplantation Porcine Embryos Derived In Vitro or by Nuclear Transfer
Apoptosis occurs during preimplantation development in both in vivo- and in vitro-produced embryos, and it may contribute to embryonic loss. The present study investigated the development of porcine nuclear transfer (NT) embryos reconstructed by using fetal fibroblasts as compared to embryos produce...
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| Veröffentlicht in: | Biology of reproduction 2003-08, Vol.69 (2), p.501-507 |
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| creator | YANHONG HAO LIANGXUE LAI JIUDE MAO IM, Gi-Sun BONK, Aaron PRATHER, Randall S |
| description | Apoptosis occurs during preimplantation development in both in vivo- and in vitro-produced embryos, and it may contribute
to embryonic loss. The present study investigated the development of porcine nuclear transfer (NT) embryos reconstructed by
using fetal fibroblasts as compared to embryos produced by in vitro fertilization (IVF). The onset and the frequency of apoptosis
in NT and IVF embryos were examined via morphological and nuclear changes and TUNEL assay. The NT blastocysts had a similar
number of nuclei as compared to IVF blastocysts and appeared to be morphologically similar. Relative to IVF embryos, the NT
embryos had a lower cleavage rate (42.7% vs. 71.0%) and a lower developmental rate (11.1% vs. 28.6%) to the blastocyst stage.
The earliest positive TUNEL signals were detected in the NT embryos on Day 5 of culture. The percentage of cells undergoing
apoptosis in the NT embryos was higher than that of the IVF embryos and increased with time in vitro. Some of the abnormal
morphological changes observed during early development related to apoptosis. Cytoplasmic fragmentation, developmental arrest,
and nuclear condensation were typical characteristics of embryos undergoing apoptosis. Some mechanisms of the apoptotic pathway
were triggered by changes in the NT embryos. The developmental rates of NT embryos might be improved by identifying specific
apoptotic pathways and then intervening in these pathways to improve development. |
| doi_str_mv | 10.1095/biolreprod.103.016170 |
| format | Article |
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to embryonic loss. The present study investigated the development of porcine nuclear transfer (NT) embryos reconstructed by
using fetal fibroblasts as compared to embryos produced by in vitro fertilization (IVF). The onset and the frequency of apoptosis
in NT and IVF embryos were examined via morphological and nuclear changes and TUNEL assay. The NT blastocysts had a similar
number of nuclei as compared to IVF blastocysts and appeared to be morphologically similar. Relative to IVF embryos, the NT
embryos had a lower cleavage rate (42.7% vs. 71.0%) and a lower developmental rate (11.1% vs. 28.6%) to the blastocyst stage.
The earliest positive TUNEL signals were detected in the NT embryos on Day 5 of culture. The percentage of cells undergoing
apoptosis in the NT embryos was higher than that of the IVF embryos and increased with time in vitro. Some of the abnormal
morphological changes observed during early development related to apoptosis. Cytoplasmic fragmentation, developmental arrest,
and nuclear condensation were typical characteristics of embryos undergoing apoptosis. Some mechanisms of the apoptotic pathway
were triggered by changes in the NT embryos. The developmental rates of NT embryos might be improved by identifying specific
apoptotic pathways and then intervening in these pathways to improve development.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod.103.016170</identifier><identifier>PMID: 12700186</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>Animals ; Apoptosis - physiology ; Biological and medical sciences ; Birth control ; Blastocyst - physiology ; Cell Nucleus - genetics ; Embryonic and Fetal Development - physiology ; Female ; Fertilization in Vitro ; Gynecology. Andrology. Obstetrics ; In Situ Nick-End Labeling ; Medical sciences ; Oocytes - growth & development ; Oocytes - physiology ; Pregnancy ; Sterility. Assisted procreation ; Swine</subject><ispartof>Biology of reproduction, 2003-08, Vol.69 (2), p.501-507</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27929,27930</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15329310$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12700186$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>YANHONG HAO</creatorcontrib><creatorcontrib>LIANGXUE LAI</creatorcontrib><creatorcontrib>JIUDE MAO</creatorcontrib><creatorcontrib>IM, Gi-Sun</creatorcontrib><creatorcontrib>BONK, Aaron</creatorcontrib><creatorcontrib>PRATHER, Randall S</creatorcontrib><title>Apoptosis and In Vitro Development of Preimplantation Porcine Embryos Derived In Vitro or by Nuclear Transfer</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>Apoptosis occurs during preimplantation development in both in vivo- and in vitro-produced embryos, and it may contribute
to embryonic loss. The present study investigated the development of porcine nuclear transfer (NT) embryos reconstructed by
using fetal fibroblasts as compared to embryos produced by in vitro fertilization (IVF). The onset and the frequency of apoptosis
in NT and IVF embryos were examined via morphological and nuclear changes and TUNEL assay. The NT blastocysts had a similar
number of nuclei as compared to IVF blastocysts and appeared to be morphologically similar. Relative to IVF embryos, the NT
embryos had a lower cleavage rate (42.7% vs. 71.0%) and a lower developmental rate (11.1% vs. 28.6%) to the blastocyst stage.
The earliest positive TUNEL signals were detected in the NT embryos on Day 5 of culture. The percentage of cells undergoing
apoptosis in the NT embryos was higher than that of the IVF embryos and increased with time in vitro. Some of the abnormal
morphological changes observed during early development related to apoptosis. Cytoplasmic fragmentation, developmental arrest,
and nuclear condensation were typical characteristics of embryos undergoing apoptosis. Some mechanisms of the apoptotic pathway
were triggered by changes in the NT embryos. The developmental rates of NT embryos might be improved by identifying specific
apoptotic pathways and then intervening in these pathways to improve development.</description><subject>Animals</subject><subject>Apoptosis - physiology</subject><subject>Biological and medical sciences</subject><subject>Birth control</subject><subject>Blastocyst - physiology</subject><subject>Cell Nucleus - genetics</subject><subject>Embryonic and Fetal Development - physiology</subject><subject>Female</subject><subject>Fertilization in Vitro</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>In Situ Nick-End Labeling</subject><subject>Medical sciences</subject><subject>Oocytes - growth & development</subject><subject>Oocytes - physiology</subject><subject>Pregnancy</subject><subject>Sterility. Assisted procreation</subject><subject>Swine</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpN0clKBDEQBuAgio7LIyi56K01yyQxR9FxAVEP6rVJpytOJN1pkx6HeXsDjsup-OGrgqpC6JCSU0q0OGt8DAmGFNuS-SmhkiqygSZUMF0pJs830YQQIivOJd9Buzm_E0KnnPFttEOZKuFcTlB3McRhjNlnbPoW3_X41Y8p4iv4hBCHDvoRR4efEvhuCKYfzehjj59isr4HPOuatIq58OQ_4V9_TLhZ4YeFDWASfk6mzw7SPtpyJmQ4WNc99HI9e768re4fb-4uL-6rOZNqrBSZsqmwRiqjjNWNk8ppwhrpJGm5VcCVcC3VU0Za47RsWAsatHRGWSKo43vo5Htuuc_HAvJYdz5bCGUBiItcKy6KY6LAozVcNB209ZB8Z9Kq_jlQAcdrYLI1wZVFrM9_TnCmeXnAr5v7t_nSJ6hzZ0IoY3m9XC6lrlktCOVfBmiFqw</recordid><startdate>20030801</startdate><enddate>20030801</enddate><creator>YANHONG HAO</creator><creator>LIANGXUE LAI</creator><creator>JIUDE MAO</creator><creator>IM, Gi-Sun</creator><creator>BONK, Aaron</creator><creator>PRATHER, Randall S</creator><general>Society for the Study of Reproduction</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20030801</creationdate><title>Apoptosis and In Vitro Development of Preimplantation Porcine Embryos Derived In Vitro or by Nuclear Transfer</title><author>YANHONG HAO ; LIANGXUE LAI ; JIUDE MAO ; IM, Gi-Sun ; BONK, Aaron ; PRATHER, Randall S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h267t-704245ca67a7ac9bf67f902b6f60d3c7e375fd19420daf96b2de9e96fa7c051f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Apoptosis - physiology</topic><topic>Biological and medical sciences</topic><topic>Birth control</topic><topic>Blastocyst - physiology</topic><topic>Cell Nucleus - genetics</topic><topic>Embryonic and Fetal Development - physiology</topic><topic>Female</topic><topic>Fertilization in Vitro</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>In Situ Nick-End Labeling</topic><topic>Medical sciences</topic><topic>Oocytes - growth & development</topic><topic>Oocytes - physiology</topic><topic>Pregnancy</topic><topic>Sterility. Assisted procreation</topic><topic>Swine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>YANHONG HAO</creatorcontrib><creatorcontrib>LIANGXUE LAI</creatorcontrib><creatorcontrib>JIUDE MAO</creatorcontrib><creatorcontrib>IM, Gi-Sun</creatorcontrib><creatorcontrib>BONK, Aaron</creatorcontrib><creatorcontrib>PRATHER, Randall S</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>YANHONG HAO</au><au>LIANGXUE LAI</au><au>JIUDE MAO</au><au>IM, Gi-Sun</au><au>BONK, Aaron</au><au>PRATHER, Randall S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Apoptosis and In Vitro Development of Preimplantation Porcine Embryos Derived In Vitro or by Nuclear Transfer</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>2003-08-01</date><risdate>2003</risdate><volume>69</volume><issue>2</issue><spage>501</spage><epage>507</epage><pages>501-507</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>Apoptosis occurs during preimplantation development in both in vivo- and in vitro-produced embryos, and it may contribute
to embryonic loss. The present study investigated the development of porcine nuclear transfer (NT) embryos reconstructed by
using fetal fibroblasts as compared to embryos produced by in vitro fertilization (IVF). The onset and the frequency of apoptosis
in NT and IVF embryos were examined via morphological and nuclear changes and TUNEL assay. The NT blastocysts had a similar
number of nuclei as compared to IVF blastocysts and appeared to be morphologically similar. Relative to IVF embryos, the NT
embryos had a lower cleavage rate (42.7% vs. 71.0%) and a lower developmental rate (11.1% vs. 28.6%) to the blastocyst stage.
The earliest positive TUNEL signals were detected in the NT embryos on Day 5 of culture. The percentage of cells undergoing
apoptosis in the NT embryos was higher than that of the IVF embryos and increased with time in vitro. Some of the abnormal
morphological changes observed during early development related to apoptosis. Cytoplasmic fragmentation, developmental arrest,
and nuclear condensation were typical characteristics of embryos undergoing apoptosis. Some mechanisms of the apoptotic pathway
were triggered by changes in the NT embryos. The developmental rates of NT embryos might be improved by identifying specific
apoptotic pathways and then intervening in these pathways to improve development.</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>12700186</pmid><doi>10.1095/biolreprod.103.016170</doi><tpages>7</tpages></addata></record> |
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| ispartof | Biology of reproduction, 2003-08, Vol.69 (2), p.501-507 |
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| language | eng |
| recordid | cdi_proquest_miscellaneous_73505125 |
| source | MEDLINE; BioOne Complete; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Apoptosis - physiology Biological and medical sciences Birth control Blastocyst - physiology Cell Nucleus - genetics Embryonic and Fetal Development - physiology Female Fertilization in Vitro Gynecology. Andrology. Obstetrics In Situ Nick-End Labeling Medical sciences Oocytes - growth & development Oocytes - physiology Pregnancy Sterility. Assisted procreation Swine |
| title | Apoptosis and In Vitro Development of Preimplantation Porcine Embryos Derived In Vitro or by Nuclear Transfer |
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