Complementary DNA Cloning and Characterization of Rat spergen-2, a Spermatogenic Cell-Specific Gene 2 Encoding a 56-Kilodalton Nuclear Protein Bearing Ankyrin Repeat Motifs

Differential display in combination with a cDNA cloning approach were used to isolate a novel gene, spergen-2, which has an open reading frame of 1500 nucleotides and encodes a protein of 500 amino acids that contains ankyrin repeat motifs and a putative nuclear localization signal. Expression of sp...

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Veröffentlicht in:	Biology of reproduction 2003-08, Vol.69 (2), p.421-429
Hauptverfasser:	Iida, Hiroshi, Urasoko, Aiko, Doiguchi, Masamichi, Mōri, Takayuki, Toshimori, Kiyotaka, Shibata, Yosaburo
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Ankyrins - biosynthesis Ankyrins - genetics Biological and medical sciences Blotting, Northern Cloning, Molecular DNA, Complementary - biosynthesis DNA, Complementary - genetics Fundamental and applied biological sciences. Psychology Glutathione Transferase - metabolism Hormone metabolism and regulation Immunohistochemistry In Situ Hybridization Male Mammalian male genital system Molecular Sequence Data Nuclear Proteins - biosynthesis Nuclear Proteins - physiology Rats Repetitive Sequences, Nucleic Acid - genetics Repetitive Sequences, Nucleic Acid - physiology Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - biosynthesis RNA, Messenger - genetics Seminiferous Tubules - metabolism Spermatogenesis - physiology Testis - cytology Up-Regulation Vertebrates: reproduction
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