Complementary DNA Cloning and Characterization of Rat spergen-2, a Spermatogenic Cell-Specific Gene 2 Encoding a 56-Kilodalton Nuclear Protein Bearing Ankyrin Repeat Motifs
Differential display in combination with a cDNA cloning approach were used to isolate a novel gene, spergen-2, which has an open reading frame of 1500 nucleotides and encodes a protein of 500 amino acids that contains ankyrin repeat motifs and a putative nuclear localization signal. Expression of sp...
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| Veröffentlicht in: | Biology of reproduction 2003-08, Vol.69 (2), p.421-429 |
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| container_title | Biology of reproduction |
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| creator | Iida, Hiroshi Urasoko, Aiko Doiguchi, Masamichi Mōri, Takayuki Toshimori, Kiyotaka Shibata, Yosaburo |
| description | Differential display in combination with a cDNA cloning approach were used to isolate a novel gene, spergen-2, which has an open reading frame of 1500 nucleotides and encodes a protein of 500 amino acids that contains ankyrin repeat
motifs and a putative nuclear localization signal. Expression of spergen-2 is developmentally upregulated in testis. In situ hybridization revealed that spergen-2 mRNA is expressed in spermatocytes and round spermatids (steps 1â6). Immunohistochemical analysis with confocal laser-scanning
microscopy demonstrated that spergen-2 protein is predominantly expressed in nuclei of late spermatocytes (stages IXâXIV)
and spermatids (steps 1â11), indicating the restricted expression of spergen-2 during spermatogenesis. In nucleoplasm of spermatogenic
cell nuclei, spergen-2 tends to localize in the interchromosome space with relatively low DNA density. These findings indicate
a potential role of spergen-2 in spermatogenesis, especially in cell differentiation from late pachytene spermatocytes to
spermatids or in early spermatid differentiation. |
| doi_str_mv | 10.1095/biolreprod.102.013987 |
| format | Article |
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motifs and a putative nuclear localization signal. Expression of spergen-2 is developmentally upregulated in testis. In situ hybridization revealed that spergen-2 mRNA is expressed in spermatocytes and round spermatids (steps 1â6). Immunohistochemical analysis with confocal laser-scanning
microscopy demonstrated that spergen-2 protein is predominantly expressed in nuclei of late spermatocytes (stages IXâXIV)
and spermatids (steps 1â11), indicating the restricted expression of spergen-2 during spermatogenesis. In nucleoplasm of spermatogenic
cell nuclei, spergen-2 tends to localize in the interchromosome space with relatively low DNA density. These findings indicate
a potential role of spergen-2 in spermatogenesis, especially in cell differentiation from late pachytene spermatocytes to
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motifs and a putative nuclear localization signal. Expression of spergen-2 is developmentally upregulated in testis. In situ hybridization revealed that spergen-2 mRNA is expressed in spermatocytes and round spermatids (steps 1â6). Immunohistochemical analysis with confocal laser-scanning
microscopy demonstrated that spergen-2 protein is predominantly expressed in nuclei of late spermatocytes (stages IXâXIV)
and spermatids (steps 1â11), indicating the restricted expression of spergen-2 during spermatogenesis. In nucleoplasm of spermatogenic
cell nuclei, spergen-2 tends to localize in the interchromosome space with relatively low DNA density. These findings indicate
a potential role of spergen-2 in spermatogenesis, especially in cell differentiation from late pachytene spermatocytes to
spermatids or in early spermatid differentiation.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Ankyrins - biosynthesis</subject><subject>Ankyrins - genetics</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Cloning, Molecular</subject><subject>DNA, Complementary - biosynthesis</subject><subject>DNA, Complementary - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glutathione Transferase - metabolism</subject><subject>Hormone metabolism and regulation</subject><subject>Immunohistochemistry</subject><subject>In Situ Hybridization</subject><subject>Male</subject><subject>Mammalian male genital system</subject><subject>Molecular Sequence Data</subject><subject>Nuclear Proteins - biosynthesis</subject><subject>Nuclear Proteins - physiology</subject><subject>Rats</subject><subject>Repetitive Sequences, Nucleic Acid - genetics</subject><subject>Repetitive Sequences, Nucleic Acid - physiology</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - biosynthesis</subject><subject>RNA, Messenger - genetics</subject><subject>Seminiferous Tubules - metabolism</subject><subject>Spermatogenesis - physiology</subject><subject>Testis - cytology</subject><subject>Up-Regulation</subject><subject>Vertebrates: reproduction</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkN1u1DAQRi0EokvhEUC-gStS_BN7k8ttKAVRCipwHU2cya7BsYPt1ao8Ew-JgUW9Gn-jozPyR8hTzs44a9WrwQYXcYlhLFmcMS7bZn2PrLgSbbUWurlPVowxXUmp5Ql5lNI3xngthXxITrjQta7bekV-dWFeHM7oM8Rb-vp6QzsXvPVbCn6k3Q4imIzR_oRsg6dhojeQaVowbtFX4iUF-rmEGXIoC2toh85VZWXsVNIleqSCXngTxr9SqnT13rowgsvFd703DiHSTzFktJ6el_CH2_jvt-VBb3DBcu9DyHZKj8mDCVzCJ8d5Sr6-ufjSva2uPl6-6zZX1U7oda5wQNHUYlQwTBxBMWCKqRYZG5thQtUCX3PBoNFCgzF6HAc5yHbgA6hGKiVPyYt_3lLvjz2m3M82mfIv8Bj2qV_L4mO1KOCzI7gfZhz7Jdq51Nj_77cAz48AJANuiuCNTXeckqIVWt9xO7vdHWzEPs3gXNHK_nA46LYXfS24_A0Tm5sq</recordid><startdate>20030801</startdate><enddate>20030801</enddate><creator>Iida, Hiroshi</creator><creator>Urasoko, Aiko</creator><creator>Doiguchi, Masamichi</creator><creator>Mōri, Takayuki</creator><creator>Toshimori, Kiyotaka</creator><creator>Shibata, Yosaburo</creator><general>Society for the Study of Reproduction</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20030801</creationdate><title>Complementary DNA Cloning and Characterization of Rat spergen-2, a Spermatogenic Cell-Specific Gene 2 Encoding a 56-Kilodalton Nuclear Protein Bearing Ankyrin Repeat Motifs</title><author>Iida, Hiroshi ; Urasoko, Aiko ; Doiguchi, Masamichi ; Mōri, Takayuki ; Toshimori, Kiyotaka ; Shibata, Yosaburo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h267t-ebe2842d5abf1ea50a05059e00d8bfe59a17120a8626acc6ddb3b39b1ba583553</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Ankyrins - biosynthesis</topic><topic>Ankyrins - genetics</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>Cloning, Molecular</topic><topic>DNA, Complementary - biosynthesis</topic><topic>DNA, Complementary - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glutathione Transferase - metabolism</topic><topic>Hormone metabolism and regulation</topic><topic>Immunohistochemistry</topic><topic>In Situ Hybridization</topic><topic>Male</topic><topic>Mammalian male genital system</topic><topic>Molecular Sequence Data</topic><topic>Nuclear Proteins - biosynthesis</topic><topic>Nuclear Proteins - physiology</topic><topic>Rats</topic><topic>Repetitive Sequences, Nucleic Acid - genetics</topic><topic>Repetitive Sequences, Nucleic Acid - physiology</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - biosynthesis</topic><topic>RNA, Messenger - genetics</topic><topic>Seminiferous Tubules - metabolism</topic><topic>Spermatogenesis - physiology</topic><topic>Testis - cytology</topic><topic>Up-Regulation</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Iida, Hiroshi</creatorcontrib><creatorcontrib>Urasoko, Aiko</creatorcontrib><creatorcontrib>Doiguchi, Masamichi</creatorcontrib><creatorcontrib>Mōri, Takayuki</creatorcontrib><creatorcontrib>Toshimori, Kiyotaka</creatorcontrib><creatorcontrib>Shibata, Yosaburo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Iida, Hiroshi</au><au>Urasoko, Aiko</au><au>Doiguchi, Masamichi</au><au>Mōri, Takayuki</au><au>Toshimori, Kiyotaka</au><au>Shibata, Yosaburo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Complementary DNA Cloning and Characterization of Rat spergen-2, a Spermatogenic Cell-Specific Gene 2 Encoding a 56-Kilodalton Nuclear Protein Bearing Ankyrin Repeat Motifs</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>2003-08-01</date><risdate>2003</risdate><volume>69</volume><issue>2</issue><spage>421</spage><epage>429</epage><pages>421-429</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>Differential display in combination with a cDNA cloning approach were used to isolate a novel gene, spergen-2, which has an open reading frame of 1500 nucleotides and encodes a protein of 500 amino acids that contains ankyrin repeat
motifs and a putative nuclear localization signal. Expression of spergen-2 is developmentally upregulated in testis. In situ hybridization revealed that spergen-2 mRNA is expressed in spermatocytes and round spermatids (steps 1â6). Immunohistochemical analysis with confocal laser-scanning
microscopy demonstrated that spergen-2 protein is predominantly expressed in nuclei of late spermatocytes (stages IXâXIV)
and spermatids (steps 1â11), indicating the restricted expression of spergen-2 during spermatogenesis. In nucleoplasm of spermatogenic
cell nuclei, spergen-2 tends to localize in the interchromosome space with relatively low DNA density. These findings indicate
a potential role of spergen-2 in spermatogenesis, especially in cell differentiation from late pachytene spermatocytes to
spermatids or in early spermatid differentiation.</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>12646494</pmid><doi>10.1095/biolreprod.102.013987</doi><tpages>9</tpages></addata></record> |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; BioOne Complete |
| subjects | Amino Acid Sequence Animals Ankyrins - biosynthesis Ankyrins - genetics Biological and medical sciences Blotting, Northern Cloning, Molecular DNA, Complementary - biosynthesis DNA, Complementary - genetics Fundamental and applied biological sciences. Psychology Glutathione Transferase - metabolism Hormone metabolism and regulation Immunohistochemistry In Situ Hybridization Male Mammalian male genital system Molecular Sequence Data Nuclear Proteins - biosynthesis Nuclear Proteins - physiology Rats Repetitive Sequences, Nucleic Acid - genetics Repetitive Sequences, Nucleic Acid - physiology Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - biosynthesis RNA, Messenger - genetics Seminiferous Tubules - metabolism Spermatogenesis - physiology Testis - cytology Up-Regulation Vertebrates: reproduction |
| title | Complementary DNA Cloning and Characterization of Rat spergen-2, a Spermatogenic Cell-Specific Gene 2 Encoding a 56-Kilodalton Nuclear Protein Bearing Ankyrin Repeat Motifs |
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