Reconstruction of the 2.4 Mb human DMD-gene by homologous YAC recombination

The human dystrophin gene, mutations of which cause Duchenne and Becker muscular dystrophy, measures 2.4 Mb. This size seriously limits its cloning as a single DNA fragment and subsequent in-vitro expression studies. We have used stepwise in-vivo recombination between overlapping yeast artificial ch...

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Veröffentlicht in:	Human molecular genetics 1992-04, Vol.1 (1), p.19-28
Hauptverfasser:	DEN DUNNEN, J. T, GROOTSCHOLTEN, P. M, VAN OMMEN, G. J. B, DAUWERSE, J. G, WALKER, A. P, MONACO, A. P, BUTLER, R, ANAND, R, COFFEY, A. J, BENTLEY, D. R, STEENSMA, H. Y
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Chromosomes, Fungal Cloning, Molecular - methods Crosses, Genetic Diseases of striated muscles. Neuromuscular diseases Duchenne's muscular dystrophy dystrophin Dystrophin - genetics genes Humans In Situ Hybridization man Medical sciences Muscular Dystrophies - genetics Mutation Neurology recombination Recombination, Genetic reconstruction Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics X Chromosome
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