Correlation of Axenic Linkage Groups with the Position of the Microtubule-Organizing Center in Aggregating Dictyostelium

Positioning of the microtubule-organizing center (MTOC)in Dictyostelium discoideum was found to be genetically regulated. We examined the wild-type strain NC-4 cells independently maintained in different laboratories, freshly recovered cells from spores stocked for over 20 years, the temperature-sen...

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Veröffentlicht in:Cell Structure and Function 1992, Vol.17(6), pp.417-425
Hauptverfasser: Sameshima, Masazumi, Imai, Yoshihisa, Fujimoto, Hirotaka, Hashimoto, Yohichi
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container_issue 6
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container_title Cell Structure and Function
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creator Sameshima, Masazumi
Imai, Yoshihisa
Fujimoto, Hirotaka
Hashimoto, Yohichi
description Positioning of the microtubule-organizing center (MTOC)in Dictyostelium discoideum was found to be genetically regulated. We examined the wild-type strain NC-4 cells independently maintained in different laboratories, freshly recovered cells from spores stocked for over 20 years, the temperature-sensitive growth mutant HU49 isolated from NC-4, as well as strain V-12 which is the opposite mating-type to NC-4. During aggregation on nonnutrient agar plates, all these strains showed similar cell polarity, as denned by the alignment of the nucleus ahead of the MTOC. By contrast, in Ax2 and Ax3, axenic strains carrying axenic mutations on linkage groups II and III, the MTOC was usually positioned ahead of the nucleus. Cells containing axenic linkage group II but not III positioned the MTOC ahead of the nucleus. Conversely cell polarity of strains including axenic linkage group III but not II was similar to that of wild-type cells. Thus axenic linkage group II, probably axeC or other linked gene(s) not yet identified, is responsible for the location of the MTOC anterior to the nucleus during aggregation. The anterior positioning of the MTOCs was prevented by growth on bacteria in cells carrying both axenic linkage groups, but not in those carrying only axenic linkage group II.
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subjects Animals
axenic mutation
Biological and medical sciences
Cell Aggregation
Cell Nucleus - ultrastructure
Cell Polarity
Cell structures and functions
Cytoskeleton, cytoplasm. Intracellular movements
Dictyostelium
Dictyostelium - genetics
Dictyostelium - growth & development
Dictyostelium - ultrastructure
Fundamental and applied biological sciences. Psychology
Genes, Fungal
Genes, Mating Type, Fungal
Genes, Recessive
Genetic Linkage
microtubule-organizing center
Microtubules - ultrastructure
Molecular and cellular biology
nucleus
Phagocytosis
rapid-freeze substitution
title Correlation of Axenic Linkage Groups with the Position of the Microtubule-Organizing Center in Aggregating Dictyostelium
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