Correlation of Axenic Linkage Groups with the Position of the Microtubule-Organizing Center in Aggregating Dictyostelium
Positioning of the microtubule-organizing center (MTOC)in Dictyostelium discoideum was found to be genetically regulated. We examined the wild-type strain NC-4 cells independently maintained in different laboratories, freshly recovered cells from spores stocked for over 20 years, the temperature-sen...
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Veröffentlicht in: | Cell Structure and Function 1992, Vol.17(6), pp.417-425 |
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description | Positioning of the microtubule-organizing center (MTOC)in Dictyostelium discoideum was found to be genetically regulated. We examined the wild-type strain NC-4 cells independently maintained in different laboratories, freshly recovered cells from spores stocked for over 20 years, the temperature-sensitive growth mutant HU49 isolated from NC-4, as well as strain V-12 which is the opposite mating-type to NC-4. During aggregation on nonnutrient agar plates, all these strains showed similar cell polarity, as denned by the alignment of the nucleus ahead of the MTOC. By contrast, in Ax2 and Ax3, axenic strains carrying axenic mutations on linkage groups II and III, the MTOC was usually positioned ahead of the nucleus. Cells containing axenic linkage group II but not III positioned the MTOC ahead of the nucleus. Conversely cell polarity of strains including axenic linkage group III but not II was similar to that of wild-type cells. Thus axenic linkage group II, probably axeC or other linked gene(s) not yet identified, is responsible for the location of the MTOC anterior to the nucleus during aggregation. The anterior positioning of the MTOCs was prevented by growth on bacteria in cells carrying both axenic linkage groups, but not in those carrying only axenic linkage group II. |
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We examined the wild-type strain NC-4 cells independently maintained in different laboratories, freshly recovered cells from spores stocked for over 20 years, the temperature-sensitive growth mutant HU49 isolated from NC-4, as well as strain V-12 which is the opposite mating-type to NC-4. During aggregation on nonnutrient agar plates, all these strains showed similar cell polarity, as denned by the alignment of the nucleus ahead of the MTOC. By contrast, in Ax2 and Ax3, axenic strains carrying axenic mutations on linkage groups II and III, the MTOC was usually positioned ahead of the nucleus. Cells containing axenic linkage group II but not III positioned the MTOC ahead of the nucleus. Conversely cell polarity of strains including axenic linkage group III but not II was similar to that of wild-type cells. Thus axenic linkage group II, probably axeC or other linked gene(s) not yet identified, is responsible for the location of the MTOC anterior to the nucleus during aggregation. The anterior positioning of the MTOCs was prevented by growth on bacteria in cells carrying both axenic linkage groups, but not in those carrying only axenic linkage group II.</description><identifier>ISSN: 0386-7196</identifier><identifier>EISSN: 1347-3700</identifier><identifier>DOI: 10.1247/csf.17.417</identifier><identifier>PMID: 1295696</identifier><identifier>CODEN: CSFUDY</identifier><language>eng</language><publisher>Kyoto: Japan Society for Cell Biology</publisher><subject>Animals ; axenic mutation ; Biological and medical sciences ; Cell Aggregation ; Cell Nucleus - ultrastructure ; Cell Polarity ; Cell structures and functions ; Cytoskeleton, cytoplasm. Intracellular movements ; Dictyostelium ; Dictyostelium - genetics ; Dictyostelium - growth & development ; Dictyostelium - ultrastructure ; Fundamental and applied biological sciences. Psychology ; Genes, Fungal ; Genes, Mating Type, Fungal ; Genes, Recessive ; Genetic Linkage ; microtubule-organizing center ; Microtubules - ultrastructure ; Molecular and cellular biology ; nucleus ; Phagocytosis ; rapid-freeze substitution</subject><ispartof>Cell Structure and Function, 1992, Vol.17(6), pp.417-425</ispartof><rights>Japan Society for Cell Biology</rights><rights>1993 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 1992</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c530t-f8dedae52a0ed04cada7c111e368ba70ad1d7e6c34f0d14b3a7c7454037880273</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>309,310,314,776,780,785,786,1877,23909,23910,25118,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4658586$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1295696$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sameshima, Masazumi</creatorcontrib><creatorcontrib>Imai, Yoshihisa</creatorcontrib><creatorcontrib>Fujimoto, Hirotaka</creatorcontrib><creatorcontrib>Hashimoto, Yohichi</creatorcontrib><title>Correlation of Axenic Linkage Groups with the Position of the Microtubule-Organizing Center in Aggregating Dictyostelium</title><title>Cell Structure and Function</title><addtitle>Cell Struct. Funct.</addtitle><description>Positioning of the microtubule-organizing center (MTOC)in Dictyostelium discoideum was found to be genetically regulated. We examined the wild-type strain NC-4 cells independently maintained in different laboratories, freshly recovered cells from spores stocked for over 20 years, the temperature-sensitive growth mutant HU49 isolated from NC-4, as well as strain V-12 which is the opposite mating-type to NC-4. During aggregation on nonnutrient agar plates, all these strains showed similar cell polarity, as denned by the alignment of the nucleus ahead of the MTOC. By contrast, in Ax2 and Ax3, axenic strains carrying axenic mutations on linkage groups II and III, the MTOC was usually positioned ahead of the nucleus. Cells containing axenic linkage group II but not III positioned the MTOC ahead of the nucleus. Conversely cell polarity of strains including axenic linkage group III but not II was similar to that of wild-type cells. Thus axenic linkage group II, probably axeC or other linked gene(s) not yet identified, is responsible for the location of the MTOC anterior to the nucleus during aggregation. The anterior positioning of the MTOCs was prevented by growth on bacteria in cells carrying both axenic linkage groups, but not in those carrying only axenic linkage group II.</description><subject>Animals</subject><subject>axenic mutation</subject><subject>Biological and medical sciences</subject><subject>Cell Aggregation</subject><subject>Cell Nucleus - ultrastructure</subject><subject>Cell Polarity</subject><subject>Cell structures and functions</subject><subject>Cytoskeleton, cytoplasm. Intracellular movements</subject><subject>Dictyostelium</subject><subject>Dictyostelium - genetics</subject><subject>Dictyostelium - growth & development</subject><subject>Dictyostelium - ultrastructure</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Fungal</subject><subject>Genes, Mating Type, Fungal</subject><subject>Genes, Recessive</subject><subject>Genetic Linkage</subject><subject>microtubule-organizing center</subject><subject>Microtubules - ultrastructure</subject><subject>Molecular and cellular biology</subject><subject>nucleus</subject><subject>Phagocytosis</subject><subject>rapid-freeze substitution</subject><issn>0386-7196</issn><issn>1347-3700</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkc-L1DAcxYMo6-zqxbsQUPYgdEyaX-1xqLoKI-tBzyWTftvJ2EnGJMVd_3pTOu6Cl4TkfXjvmzyEXlGypiVX703s11StOVVP0IoyrgqmCHmKVoRVslC0ls_RZYwHQkpBpLpAF7SshazlCt01PgQYdbLeYd_jzR04a_DWup96AHwT_HSK-LdNe5z2gL_5aP-h8_mrNcGnaTeNUNyGQTv7x7oBN-ASBGwd3gxDgCHb59sP1qR7HxOMdjq-QM96PUZ4ed6v0I9PH783n4vt7c2XZrMtjGAkFX3VQadBlJpAR7jRnVaGUgpMVjutiO5op0AaxnvSUb5jWVZccMJUVZFSsSt0vfiegv81QUzt0UYD46gd-Cm2ivFaUVJl8M1_4MFPweXZWspFLbiQoszUu4XK744xQN-egj3qcN9S0s5ltLmMlqo2l5Hh12fLaXeE7hFdfj_rb8-6jkaPfdDO2PiAcSkqUc1Ys2CHmHIpD7oOyZoR5kRaKzGnymXJ4Y_qXocWHPsLwk2q6A</recordid><startdate>19921201</startdate><enddate>19921201</enddate><creator>Sameshima, Masazumi</creator><creator>Imai, Yoshihisa</creator><creator>Fujimoto, Hirotaka</creator><creator>Hashimoto, Yohichi</creator><general>Japan Society for Cell Biology</general><general>Japan Science and Technology Agency</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19921201</creationdate><title>Correlation of Axenic Linkage Groups with the Position of the Microtubule-Organizing Center in Aggregating Dictyostelium</title><author>Sameshima, Masazumi ; Imai, Yoshihisa ; Fujimoto, Hirotaka ; Hashimoto, Yohichi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c530t-f8dedae52a0ed04cada7c111e368ba70ad1d7e6c34f0d14b3a7c7454037880273</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Animals</topic><topic>axenic mutation</topic><topic>Biological and medical sciences</topic><topic>Cell Aggregation</topic><topic>Cell Nucleus - ultrastructure</topic><topic>Cell Polarity</topic><topic>Cell structures and functions</topic><topic>Cytoskeleton, cytoplasm. Intracellular movements</topic><topic>Dictyostelium</topic><topic>Dictyostelium - genetics</topic><topic>Dictyostelium - growth & development</topic><topic>Dictyostelium - ultrastructure</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Fungal</topic><topic>Genes, Mating Type, Fungal</topic><topic>Genes, Recessive</topic><topic>Genetic Linkage</topic><topic>microtubule-organizing center</topic><topic>Microtubules - ultrastructure</topic><topic>Molecular and cellular biology</topic><topic>nucleus</topic><topic>Phagocytosis</topic><topic>rapid-freeze substitution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sameshima, Masazumi</creatorcontrib><creatorcontrib>Imai, Yoshihisa</creatorcontrib><creatorcontrib>Fujimoto, Hirotaka</creatorcontrib><creatorcontrib>Hashimoto, Yohichi</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cell Structure and Function</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sameshima, Masazumi</au><au>Imai, Yoshihisa</au><au>Fujimoto, Hirotaka</au><au>Hashimoto, Yohichi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Correlation of Axenic Linkage Groups with the Position of the Microtubule-Organizing Center in Aggregating Dictyostelium</atitle><jtitle>Cell Structure and Function</jtitle><addtitle>Cell Struct. Funct.</addtitle><date>1992-12-01</date><risdate>1992</risdate><volume>17</volume><issue>6</issue><spage>417</spage><epage>425</epage><pages>417-425</pages><issn>0386-7196</issn><eissn>1347-3700</eissn><coden>CSFUDY</coden><abstract>Positioning of the microtubule-organizing center (MTOC)in Dictyostelium discoideum was found to be genetically regulated. We examined the wild-type strain NC-4 cells independently maintained in different laboratories, freshly recovered cells from spores stocked for over 20 years, the temperature-sensitive growth mutant HU49 isolated from NC-4, as well as strain V-12 which is the opposite mating-type to NC-4. During aggregation on nonnutrient agar plates, all these strains showed similar cell polarity, as denned by the alignment of the nucleus ahead of the MTOC. By contrast, in Ax2 and Ax3, axenic strains carrying axenic mutations on linkage groups II and III, the MTOC was usually positioned ahead of the nucleus. Cells containing axenic linkage group II but not III positioned the MTOC ahead of the nucleus. Conversely cell polarity of strains including axenic linkage group III but not II was similar to that of wild-type cells. Thus axenic linkage group II, probably axeC or other linked gene(s) not yet identified, is responsible for the location of the MTOC anterior to the nucleus during aggregation. The anterior positioning of the MTOCs was prevented by growth on bacteria in cells carrying both axenic linkage groups, but not in those carrying only axenic linkage group II.</abstract><cop>Kyoto</cop><pub>Japan Society for Cell Biology</pub><pmid>1295696</pmid><doi>10.1247/csf.17.417</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Animals axenic mutation Biological and medical sciences Cell Aggregation Cell Nucleus - ultrastructure Cell Polarity Cell structures and functions Cytoskeleton, cytoplasm. Intracellular movements Dictyostelium Dictyostelium - genetics Dictyostelium - growth & development Dictyostelium - ultrastructure Fundamental and applied biological sciences. Psychology Genes, Fungal Genes, Mating Type, Fungal Genes, Recessive Genetic Linkage microtubule-organizing center Microtubules - ultrastructure Molecular and cellular biology nucleus Phagocytosis rapid-freeze substitution |
title | Correlation of Axenic Linkage Groups with the Position of the Microtubule-Organizing Center in Aggregating Dictyostelium |
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