Intimal proliferation in an organ culture of human internal mammary artery
Objective: Intimal smooth muscle cell proliferation is an early feature of atherosclerosis. Its progression is difficult to monitor in humans and previous studies have mostly relied on necropsy material. The aim of this study was therefore to establish whether intimal proliferation occurred in an or...
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| Veröffentlicht in: | Cardiovascular research 1992-12, Vol.26 (12), p.1189-1194 |
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| container_title | Cardiovascular research |
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| creator | Holt, Cathy M Francis, Sheila E Rogers, Suzanne Gadsdon, Patricia A Taylor, Trevor Clelland, Colin Soyombo, Abigail Newby, Andrew C Angelini, Gianni D |
| description | Objective: Intimal smooth muscle cell proliferation is an early feature of atherosclerosis. Its progression is difficult to monitor in humans and previous studies have mostly relied on necropsy material. The aim of this study was therefore to establish whether intimal proliferation occurred in an organ culture of human internal mammary artery. Methods: Segments of freshly isolated internal mammary artery were maintained in standard tissue culture medium containing 30% calf serum for 14 d. Tissue viability (measured by ATP concentration) was maintained during processing and throughout the culture period [211(SEM 28) nmol ATP·g−1 wet weight on d 1 v 208(27) on d 14]. Results: Histological transverse sections of cultured internal mammary artery showed the development of α neointima containing smooth muscle cells identified by immunocytochemistry for a actin. Pulse labelling of cultures with [3H]-thymidine showed proliferating cells predominantly in a neointimal layer with few dividing cells in the media. Cultured de-endothelialised vessels showed less neointimal thickening than cultured freshly isolated vessels [16(3) v 36(5) μm, p |
| doi_str_mv | 10.1093/cvr/26.12.1189 |
| format | Article |
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Its progression is difficult to monitor in humans and previous studies have mostly relied on necropsy material. The aim of this study was therefore to establish whether intimal proliferation occurred in an organ culture of human internal mammary artery. Methods: Segments of freshly isolated internal mammary artery were maintained in standard tissue culture medium containing 30% calf serum for 14 d. Tissue viability (measured by ATP concentration) was maintained during processing and throughout the culture period [211(SEM 28) nmol ATP·g−1 wet weight on d 1 v 208(27) on d 14]. Results: Histological transverse sections of cultured internal mammary artery showed the development of α neointima containing smooth muscle cells identified by immunocytochemistry for a actin. Pulse labelling of cultures with [3H]-thymidine showed proliferating cells predominantly in a neointimal layer with few dividing cells in the media. Cultured de-endothelialised vessels showed less neointimal thickening than cultured freshly isolated vessels [16(3) v 36(5) μm, p<0.0025] as well as a reduced number of dividing cells per mm of neointimal length [3.1(0.6) v 5.5(1.1), p<0.05]. Conclusions: Intimal proliferation occurred in organ culture of internal mammary artery. There is evidence for a factor derived from the endothelium, which may be important in the development of intimal proliferation. Cardiovascular Research 1992;26:1189-1194</description><identifier>ISSN: 0008-6363</identifier><identifier>EISSN: 1755-3245</identifier><identifier>DOI: 10.1093/cvr/26.12.1189</identifier><identifier>PMID: 1288865</identifier><identifier>CODEN: CVREAU</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Aged ; Arteriosclerosis - pathology ; Atherosclerosis (general aspects, experimental research) ; Biological and medical sciences ; Blood and lymphatic vessels ; Cardiology. Vascular system ; Cell Division - physiology ; Culture Techniques ; Endothelium - cytology ; Endothelium - ultrastructure ; Female ; Humans ; Immunohistochemistry ; internal mammary artery ; intimal proliferation ; Male ; Mammary Arteries - cytology ; Mammary Arteries - ultrastructure ; Medical sciences ; Microscopy, Electron ; Middle Aged ; Muscle, Smooth - cytology ; organ culture ; Tunica Intima - cytology ; Tunica Intima - ultrastructure</subject><ispartof>Cardiovascular research, 1992-12, Vol.26 (12), p.1189-1194</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c427t-195f1333fb31ea4b3f51a81aba67a785f6d061eb2b44b27b9d393113a952e16f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4507620$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1288865$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Holt, Cathy M</creatorcontrib><creatorcontrib>Francis, Sheila E</creatorcontrib><creatorcontrib>Rogers, Suzanne</creatorcontrib><creatorcontrib>Gadsdon, Patricia A</creatorcontrib><creatorcontrib>Taylor, Trevor</creatorcontrib><creatorcontrib>Clelland, Colin</creatorcontrib><creatorcontrib>Soyombo, Abigail</creatorcontrib><creatorcontrib>Newby, Andrew C</creatorcontrib><creatorcontrib>Angelini, Gianni D</creatorcontrib><title>Intimal proliferation in an organ culture of human internal mammary artery</title><title>Cardiovascular research</title><addtitle>Cardiovasc Res</addtitle><description>Objective: Intimal smooth muscle cell proliferation is an early feature of atherosclerosis. Its progression is difficult to monitor in humans and previous studies have mostly relied on necropsy material. The aim of this study was therefore to establish whether intimal proliferation occurred in an organ culture of human internal mammary artery. Methods: Segments of freshly isolated internal mammary artery were maintained in standard tissue culture medium containing 30% calf serum for 14 d. Tissue viability (measured by ATP concentration) was maintained during processing and throughout the culture period [211(SEM 28) nmol ATP·g−1 wet weight on d 1 v 208(27) on d 14]. Results: Histological transverse sections of cultured internal mammary artery showed the development of α neointima containing smooth muscle cells identified by immunocytochemistry for a actin. Pulse labelling of cultures with [3H]-thymidine showed proliferating cells predominantly in a neointimal layer with few dividing cells in the media. Cultured de-endothelialised vessels showed less neointimal thickening than cultured freshly isolated vessels [16(3) v 36(5) μm, p<0.0025] as well as a reduced number of dividing cells per mm of neointimal length [3.1(0.6) v 5.5(1.1), p<0.05]. Conclusions: Intimal proliferation occurred in organ culture of internal mammary artery. There is evidence for a factor derived from the endothelium, which may be important in the development of intimal proliferation. Cardiovascular Research 1992;26:1189-1194</description><subject>Aged</subject><subject>Arteriosclerosis - pathology</subject><subject>Atherosclerosis (general aspects, experimental research)</subject><subject>Biological and medical sciences</subject><subject>Blood and lymphatic vessels</subject><subject>Cardiology. Vascular system</subject><subject>Cell Division - physiology</subject><subject>Culture Techniques</subject><subject>Endothelium - cytology</subject><subject>Endothelium - ultrastructure</subject><subject>Female</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>internal mammary artery</subject><subject>intimal proliferation</subject><subject>Male</subject><subject>Mammary Arteries - cytology</subject><subject>Mammary Arteries - ultrastructure</subject><subject>Medical sciences</subject><subject>Microscopy, Electron</subject><subject>Middle Aged</subject><subject>Muscle, Smooth - cytology</subject><subject>organ culture</subject><subject>Tunica Intima - cytology</subject><subject>Tunica Intima - ultrastructure</subject><issn>0008-6363</issn><issn>1755-3245</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkElPxDAMhSMEgmG5ckPqAXHrEMdN0h7RiB2JC5u4RG4ngUIXSFoE_56MZgQXW_b7_GQ9xvaBT4EXeFx9-WOhpiCmAHmxxiagpUxRZHKdTTjneapQ4RbbDuEtjlLqbJNtgsjzXMkJu7rshrqlJvnwfVM762mo-y6pu4S6pPcvsVZjM4zeJr1LXseWFuJgfRdvWmpb8j8J-bj42WUbjppg91Z9h92fnd7NLtKb2_PL2clNWmVCDykU0gEiuhLBUlaik0A5UElKk86lU3OuwJaizLJS6LKYY4EASIUUFpTDHXa09I0vf442DKatQ2Wbhjrbj8FozAqBCBGcLsHK9yF468yHrxcPG-BmEZ6J4RmhDAizCC8eHKycx7K18398mVbUD1c6hYoa56mr6vCHZZJrJXjE0iVWh8F-_8nk343SqKW5eHo2evaY84fHa3ONv7Iohq8</recordid><startdate>19921201</startdate><enddate>19921201</enddate><creator>Holt, Cathy M</creator><creator>Francis, Sheila E</creator><creator>Rogers, Suzanne</creator><creator>Gadsdon, Patricia A</creator><creator>Taylor, Trevor</creator><creator>Clelland, Colin</creator><creator>Soyombo, Abigail</creator><creator>Newby, Andrew C</creator><creator>Angelini, Gianni D</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19921201</creationdate><title>Intimal proliferation in an organ culture of human internal mammary artery</title><author>Holt, Cathy M ; Francis, Sheila E ; Rogers, Suzanne ; Gadsdon, Patricia A ; Taylor, Trevor ; Clelland, Colin ; Soyombo, Abigail ; Newby, Andrew C ; Angelini, Gianni D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c427t-195f1333fb31ea4b3f51a81aba67a785f6d061eb2b44b27b9d393113a952e16f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Aged</topic><topic>Arteriosclerosis - pathology</topic><topic>Atherosclerosis (general aspects, experimental research)</topic><topic>Biological and medical sciences</topic><topic>Blood and lymphatic vessels</topic><topic>Cardiology. Vascular system</topic><topic>Cell Division - physiology</topic><topic>Culture Techniques</topic><topic>Endothelium - cytology</topic><topic>Endothelium - ultrastructure</topic><topic>Female</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>internal mammary artery</topic><topic>intimal proliferation</topic><topic>Male</topic><topic>Mammary Arteries - cytology</topic><topic>Mammary Arteries - ultrastructure</topic><topic>Medical sciences</topic><topic>Microscopy, Electron</topic><topic>Middle Aged</topic><topic>Muscle, Smooth - cytology</topic><topic>organ culture</topic><topic>Tunica Intima - cytology</topic><topic>Tunica Intima - ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Holt, Cathy M</creatorcontrib><creatorcontrib>Francis, Sheila E</creatorcontrib><creatorcontrib>Rogers, Suzanne</creatorcontrib><creatorcontrib>Gadsdon, Patricia A</creatorcontrib><creatorcontrib>Taylor, Trevor</creatorcontrib><creatorcontrib>Clelland, Colin</creatorcontrib><creatorcontrib>Soyombo, Abigail</creatorcontrib><creatorcontrib>Newby, Andrew C</creatorcontrib><creatorcontrib>Angelini, Gianni D</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cardiovascular research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Holt, Cathy M</au><au>Francis, Sheila E</au><au>Rogers, Suzanne</au><au>Gadsdon, Patricia A</au><au>Taylor, Trevor</au><au>Clelland, Colin</au><au>Soyombo, Abigail</au><au>Newby, Andrew C</au><au>Angelini, Gianni D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Intimal proliferation in an organ culture of human internal mammary artery</atitle><jtitle>Cardiovascular research</jtitle><addtitle>Cardiovasc Res</addtitle><date>1992-12-01</date><risdate>1992</risdate><volume>26</volume><issue>12</issue><spage>1189</spage><epage>1194</epage><pages>1189-1194</pages><issn>0008-6363</issn><eissn>1755-3245</eissn><coden>CVREAU</coden><abstract>Objective: Intimal smooth muscle cell proliferation is an early feature of atherosclerosis. Its progression is difficult to monitor in humans and previous studies have mostly relied on necropsy material. The aim of this study was therefore to establish whether intimal proliferation occurred in an organ culture of human internal mammary artery. Methods: Segments of freshly isolated internal mammary artery were maintained in standard tissue culture medium containing 30% calf serum for 14 d. Tissue viability (measured by ATP concentration) was maintained during processing and throughout the culture period [211(SEM 28) nmol ATP·g−1 wet weight on d 1 v 208(27) on d 14]. Results: Histological transverse sections of cultured internal mammary artery showed the development of α neointima containing smooth muscle cells identified by immunocytochemistry for a actin. Pulse labelling of cultures with [3H]-thymidine showed proliferating cells predominantly in a neointimal layer with few dividing cells in the media. Cultured de-endothelialised vessels showed less neointimal thickening than cultured freshly isolated vessels [16(3) v 36(5) μm, p<0.0025] as well as a reduced number of dividing cells per mm of neointimal length [3.1(0.6) v 5.5(1.1), p<0.05]. Conclusions: Intimal proliferation occurred in organ culture of internal mammary artery. There is evidence for a factor derived from the endothelium, which may be important in the development of intimal proliferation. Cardiovascular Research 1992;26:1189-1194</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>1288865</pmid><doi>10.1093/cvr/26.12.1189</doi><tpages>6</tpages></addata></record> |
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| subjects | Aged Arteriosclerosis - pathology Atherosclerosis (general aspects, experimental research) Biological and medical sciences Blood and lymphatic vessels Cardiology. Vascular system Cell Division - physiology Culture Techniques Endothelium - cytology Endothelium - ultrastructure Female Humans Immunohistochemistry internal mammary artery intimal proliferation Male Mammary Arteries - cytology Mammary Arteries - ultrastructure Medical sciences Microscopy, Electron Middle Aged Muscle, Smooth - cytology organ culture Tunica Intima - cytology Tunica Intima - ultrastructure |
| title | Intimal proliferation in an organ culture of human internal mammary artery |
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