Identification and Characterization of a Novel Regulatory Factor: IgA-Inducing Protein
IgA is the predominant Ig isotype in mucosal secretions and thus plays a pivotal role in host defense. The mechanisms by which IgA expression is regulated may differ among species and involve multiple pathways. Various cytokines and costimulators have been identified which regulate expression of thi...
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| Veröffentlicht in: | The Journal of immunology (1950) 2003-08, Vol.171 (3), p.1336-1342 |
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| container_title | The Journal of immunology (1950) |
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| creator | Austin, Amy S Haas, Karen M Naugler, Sasha M Bajer, Anna A Garcia-Tapia, David Estes, D. Mark |
| description | IgA is the predominant Ig isotype in mucosal secretions and thus plays a pivotal role in host defense. The mechanisms by which IgA expression is regulated may differ among species and involve multiple pathways. Various cytokines and costimulators have been identified which regulate expression of this isotype, including IL-10, IL-2, vasoactive intestinal peptide, and TGF-beta. We have tested a wide array of known factors, but only under very limited conditions do these factors mediate substantial IgA production in vitro from bovine B cells. In response to these findings, we generated a cDNA library in a mammalian expression vector from activated cells derived from bovine gut-associated lymphoid tissues (Peyer's patch and mesenteric lymph node cells) as a source of soluble factor(s) that may regulate IgA production. We have identified a novel factor, IgA-inducing protein, which stimulates relatively high levels of IgA production in vitro following CD40 stimulation in coculture with IL-2. Our data suggest that IgA-inducing protein regulates IgA by acting as a switch or differentiation factor and is expressed in a variety of lymphoid and nonlymphoid tissues. |
| doi_str_mv | 10.4049/jimmunol.171.3.1336 |
| format | Article |
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Mark</creator><creatorcontrib>Austin, Amy S ; Haas, Karen M ; Naugler, Sasha M ; Bajer, Anna A ; Garcia-Tapia, David ; Estes, D. Mark</creatorcontrib><description>IgA is the predominant Ig isotype in mucosal secretions and thus plays a pivotal role in host defense. The mechanisms by which IgA expression is regulated may differ among species and involve multiple pathways. Various cytokines and costimulators have been identified which regulate expression of this isotype, including IL-10, IL-2, vasoactive intestinal peptide, and TGF-beta. We have tested a wide array of known factors, but only under very limited conditions do these factors mediate substantial IgA production in vitro from bovine B cells. In response to these findings, we generated a cDNA library in a mammalian expression vector from activated cells derived from bovine gut-associated lymphoid tissues (Peyer's patch and mesenteric lymph node cells) as a source of soluble factor(s) that may regulate IgA production. We have identified a novel factor, IgA-inducing protein, which stimulates relatively high levels of IgA production in vitro following CD40 stimulation in coculture with IL-2. 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Mark</creatorcontrib><title>Identification and Characterization of a Novel Regulatory Factor: IgA-Inducing Protein</title><title>The Journal of immunology (1950)</title><addtitle>J Immunol</addtitle><description>IgA is the predominant Ig isotype in mucosal secretions and thus plays a pivotal role in host defense. The mechanisms by which IgA expression is regulated may differ among species and involve multiple pathways. Various cytokines and costimulators have been identified which regulate expression of this isotype, including IL-10, IL-2, vasoactive intestinal peptide, and TGF-beta. We have tested a wide array of known factors, but only under very limited conditions do these factors mediate substantial IgA production in vitro from bovine B cells. In response to these findings, we generated a cDNA library in a mammalian expression vector from activated cells derived from bovine gut-associated lymphoid tissues (Peyer's patch and mesenteric lymph node cells) as a source of soluble factor(s) that may regulate IgA production. We have identified a novel factor, IgA-inducing protein, which stimulates relatively high levels of IgA production in vitro following CD40 stimulation in coculture with IL-2. Our data suggest that IgA-inducing protein regulates IgA by acting as a switch or differentiation factor and is expressed in a variety of lymphoid and nonlymphoid tissues.</description><subject>Adjuvants, Immunologic - chemistry</subject><subject>Adjuvants, Immunologic - genetics</subject><subject>Adjuvants, Immunologic - isolation & purification</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antibodies, Anti-Idiotypic - pharmacology</subject><subject>B-Lymphocytes - immunology</subject><subject>B-Lymphocytes - metabolism</subject><subject>Base Sequence</subject><subject>Cattle</subject><subject>CD40 Antigens - physiology</subject><subject>Cells, Cultured</subject><subject>Clone Cells</subject><subject>COS Cells</subject><subject>DNA, Complementary - isolation & purification</subject><subject>Gene Library</subject><subject>GTP Phosphohydrolases - biosynthesis</subject><subject>GTP Phosphohydrolases - genetics</subject><subject>GTP Phosphohydrolases - isolation & purification</subject><subject>GTP Phosphohydrolases - metabolism</subject><subject>Humans</subject><subject>Immunoglobulin A - biosynthesis</subject><subject>Immunoglobulin M - immunology</subject><subject>Intestinal Mucosa - chemistry</subject><subject>Intestinal Mucosa - immunology</subject><subject>Intestinal Mucosa - metabolism</subject><subject>Leukocytes - immunology</subject><subject>Leukocytes - metabolism</subject><subject>Lymphoid Tissue - chemistry</subject><subject>Lymphoid Tissue - immunology</subject><subject>Lymphoid Tissue - metabolism</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Nuclear Proteins - biosynthesis</subject><subject>Nuclear Proteins - genetics</subject><subject>Nuclear Proteins - isolation & purification</subject><subject>Nuclear Proteins - metabolism</subject><subject>Organ Specificity</subject><subject>Proteins - chemistry</subject><subject>Proteins - genetics</subject><subject>Proteins - isolation & purification</subject><subject>Recombinant Proteins - pharmacology</subject><subject>Transcription, Genetic</subject><subject>Transforming Growth Factor beta - pharmacology</subject><issn>0022-1767</issn><issn>1550-6606</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkF1LwzAUhoMobn78AkF6pVedJ0mbNN6N4XQwVES9DWmbbhltM5PWMn-9HZvonVcHXp73hfMgdIFhFEEkblamqtraliPM8YiOMKXsAA1xHEPIGLBDNAQgJMSc8QE68X4FAAxIdIwGmCQ8IoQO0fss13VjCpOpxtg6UHUeTJbKqazRznztQlsEKni0n7oMXvSiLVVj3SaY9ox1t8FsMQ5ndd5mpl4Ez8422tRn6KhQpdfn-3uK3qZ3r5OHcP50P5uM52EW8bgJFSlynqqC8JhpECkruOApYJzHrBACEixyyFnGtWIsSRSmROD-ecpSrLSg9BRd7XbXzn602jeyMj7TZalqbVsvOY0E8CT-F8RJAomIeA_SHZg5673ThVw7Uym3kRjk1rv88S5775LKrfe-dbmfb9NK57-dvegeuN4BS7NYdsZp6StVlj2OZdd1f6a-AcgTjbs</recordid><startdate>20030801</startdate><enddate>20030801</enddate><creator>Austin, Amy S</creator><creator>Haas, Karen M</creator><creator>Naugler, Sasha M</creator><creator>Bajer, Anna A</creator><creator>Garcia-Tapia, David</creator><creator>Estes, D. 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Mark</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-a2fd7baf2756e09b6f797b011d56f990819d0d6c7ea6688a1329140436b1ae933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Adjuvants, Immunologic - chemistry</topic><topic>Adjuvants, Immunologic - genetics</topic><topic>Adjuvants, Immunologic - isolation & purification</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antibodies, Anti-Idiotypic - pharmacology</topic><topic>B-Lymphocytes - immunology</topic><topic>B-Lymphocytes - metabolism</topic><topic>Base Sequence</topic><topic>Cattle</topic><topic>CD40 Antigens - physiology</topic><topic>Cells, Cultured</topic><topic>Clone Cells</topic><topic>COS Cells</topic><topic>DNA, Complementary - isolation & purification</topic><topic>Gene Library</topic><topic>GTP Phosphohydrolases - biosynthesis</topic><topic>GTP Phosphohydrolases - genetics</topic><topic>GTP Phosphohydrolases - isolation & purification</topic><topic>GTP Phosphohydrolases - metabolism</topic><topic>Humans</topic><topic>Immunoglobulin A - biosynthesis</topic><topic>Immunoglobulin M - immunology</topic><topic>Intestinal Mucosa - chemistry</topic><topic>Intestinal Mucosa - immunology</topic><topic>Intestinal Mucosa - metabolism</topic><topic>Leukocytes - immunology</topic><topic>Leukocytes - metabolism</topic><topic>Lymphoid Tissue - chemistry</topic><topic>Lymphoid Tissue - immunology</topic><topic>Lymphoid Tissue - metabolism</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Nuclear Proteins - biosynthesis</topic><topic>Nuclear Proteins - genetics</topic><topic>Nuclear Proteins - isolation & purification</topic><topic>Nuclear Proteins - metabolism</topic><topic>Organ Specificity</topic><topic>Proteins - chemistry</topic><topic>Proteins - genetics</topic><topic>Proteins - isolation & purification</topic><topic>Recombinant Proteins - pharmacology</topic><topic>Transcription, Genetic</topic><topic>Transforming Growth Factor beta - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Austin, Amy S</creatorcontrib><creatorcontrib>Haas, Karen M</creatorcontrib><creatorcontrib>Naugler, Sasha M</creatorcontrib><creatorcontrib>Bajer, Anna A</creatorcontrib><creatorcontrib>Garcia-Tapia, David</creatorcontrib><creatorcontrib>Estes, D. 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| subjects | Adjuvants, Immunologic - chemistry Adjuvants, Immunologic - genetics Adjuvants, Immunologic - isolation & purification Amino Acid Sequence Animals Antibodies, Anti-Idiotypic - pharmacology B-Lymphocytes - immunology B-Lymphocytes - metabolism Base Sequence Cattle CD40 Antigens - physiology Cells, Cultured Clone Cells COS Cells DNA, Complementary - isolation & purification Gene Library GTP Phosphohydrolases - biosynthesis GTP Phosphohydrolases - genetics GTP Phosphohydrolases - isolation & purification GTP Phosphohydrolases - metabolism Humans Immunoglobulin A - biosynthesis Immunoglobulin M - immunology Intestinal Mucosa - chemistry Intestinal Mucosa - immunology Intestinal Mucosa - metabolism Leukocytes - immunology Leukocytes - metabolism Lymphoid Tissue - chemistry Lymphoid Tissue - immunology Lymphoid Tissue - metabolism Mice Molecular Sequence Data Nuclear Proteins - biosynthesis Nuclear Proteins - genetics Nuclear Proteins - isolation & purification Nuclear Proteins - metabolism Organ Specificity Proteins - chemistry Proteins - genetics Proteins - isolation & purification Recombinant Proteins - pharmacology Transcription, Genetic Transforming Growth Factor beta - pharmacology |
| title | Identification and Characterization of a Novel Regulatory Factor: IgA-Inducing Protein |
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