Selective Degradation of Cytosolic Proteins by Lysosomes
Lysosomes are able to internalize cellular proteins in a variety of ways. One pathway is selective for cytosolic proteins containing peptide sequences biochemically related to Lys-Phe-Glu-Arg-Gln (KFERQ). This pathway is activated in confluent monolayers of cultured cells in response to deprivation...
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| Veröffentlicht in: | Annals of the New York Academy of Sciences 1992-12, Vol.674 (1), p.58-64 |
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| container_title | Annals of the New York Academy of Sciences |
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| creator | DICE, J. FRED |
| description | Lysosomes are able to internalize cellular proteins in a variety of ways. One pathway is selective for cytosolic proteins containing peptide sequences biochemically related to Lys-Phe-Glu-Arg-Gln (KFERQ). This pathway is activated in confluent monolayers of cultured cells in response to deprivation of serum growth factors and applies to approximately 30% of cytosolic proteins. We have reconstituted this lysosomal degradation pathway in vitro. Uptake and/or degradation is stimulated by ATP and a member of the heat shock 70-kilodalton protein family, the 73-kilodalton constitutive heat shock protein. Several possible mechanisms of selective protein transport into lysosomes and the possible relevance of this proteolytic pathway to the processing of the amyloid precursor protein are discussed. |
| doi_str_mv | 10.1111/j.1749-6632.1992.tb27477.x |
| format | Article |
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FRED</creator><creatorcontrib>DICE, J. FRED</creatorcontrib><description>Lysosomes are able to internalize cellular proteins in a variety of ways. One pathway is selective for cytosolic proteins containing peptide sequences biochemically related to Lys-Phe-Glu-Arg-Gln (KFERQ). This pathway is activated in confluent monolayers of cultured cells in response to deprivation of serum growth factors and applies to approximately 30% of cytosolic proteins. We have reconstituted this lysosomal degradation pathway in vitro. Uptake and/or degradation is stimulated by ATP and a member of the heat shock 70-kilodalton protein family, the 73-kilodalton constitutive heat shock protein. Several possible mechanisms of selective protein transport into lysosomes and the possible relevance of this proteolytic pathway to the processing of the amyloid precursor protein are discussed.</description><identifier>ISSN: 0077-8923</identifier><identifier>EISSN: 1749-6632</identifier><identifier>DOI: 10.1111/j.1749-6632.1992.tb27477.x</identifier><identifier>PMID: 1288371</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Alzheimer Disease - metabolism ; Amino Acid Sequence ; Animals ; Cytosol - metabolism ; Heat-Shock Proteins - metabolism ; Humans ; Lysosomes - metabolism ; Molecular Sequence Data ; Proteins - metabolism ; Ribonuclease, Pancreatic</subject><ispartof>Annals of the New York Academy of Sciences, 1992-12, Vol.674 (1), p.58-64</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c352t-89435f2f22fa36783693f3f9818a9ba50c95a76584590db5a703ecf54f7cabdc3</citedby><cites>FETCH-LOGICAL-c352t-89435f2f22fa36783693f3f9818a9ba50c95a76584590db5a703ecf54f7cabdc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1288371$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>DICE, J. FRED</creatorcontrib><title>Selective Degradation of Cytosolic Proteins by Lysosomes</title><title>Annals of the New York Academy of Sciences</title><addtitle>Ann N Y Acad Sci</addtitle><description>Lysosomes are able to internalize cellular proteins in a variety of ways. One pathway is selective for cytosolic proteins containing peptide sequences biochemically related to Lys-Phe-Glu-Arg-Gln (KFERQ). This pathway is activated in confluent monolayers of cultured cells in response to deprivation of serum growth factors and applies to approximately 30% of cytosolic proteins. We have reconstituted this lysosomal degradation pathway in vitro. Uptake and/or degradation is stimulated by ATP and a member of the heat shock 70-kilodalton protein family, the 73-kilodalton constitutive heat shock protein. Several possible mechanisms of selective protein transport into lysosomes and the possible relevance of this proteolytic pathway to the processing of the amyloid precursor protein are discussed.</description><subject>Alzheimer Disease - metabolism</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Cytosol - metabolism</subject><subject>Heat-Shock Proteins - metabolism</subject><subject>Humans</subject><subject>Lysosomes - metabolism</subject><subject>Molecular Sequence Data</subject><subject>Proteins - metabolism</subject><subject>Ribonuclease, Pancreatic</subject><issn>0077-8923</issn><issn>1749-6632</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkMtOwzAQRS0EKqXwCUgRC3YJfsY2O1SgIJWHeKhLy3FslJI0xU5R8_ckSlVmM6O5985IB4ALBBPU1dUyQZzKOE0JTpCUOGkyzCnnyfYAjPfSIRhDyHksJCbH4CSEJYQIC8pHYNR1QTgaA_FuS2ua4tdGt_bL61w3Rb2KahdN26YOdVmY6NXXjS1WIcraaN6GblvZcAqOnC6DPdv1Cfi8v_uYPsTzl9nj9GYeG8Jw0z2nhDnsMHaapFyQVBJHnBRIaJlpBo1kmqdMUCZhnnUzJNY4Rh03OssNmYDL4e7a1z8bGxpVFcHYstQrW2-C4oQKljLeGa8Ho_F1CN46tfZFpX2rEFQ9NrVUPRvVs1E9NrXDprZd-Hz3ZZNVNv-PDpw6PR70IjR2u5e1_1YpJ5ypxfNMycUbhHTxpBj5A8S9eRg</recordid><startdate>19921231</startdate><enddate>19921231</enddate><creator>DICE, J. 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FRED</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c352t-89435f2f22fa36783693f3f9818a9ba50c95a76584590db5a703ecf54f7cabdc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Alzheimer Disease - metabolism</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Cytosol - metabolism</topic><topic>Heat-Shock Proteins - metabolism</topic><topic>Humans</topic><topic>Lysosomes - metabolism</topic><topic>Molecular Sequence Data</topic><topic>Proteins - metabolism</topic><topic>Ribonuclease, Pancreatic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>DICE, J. FRED</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Annals of the New York Academy of Sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>DICE, J. 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Uptake and/or degradation is stimulated by ATP and a member of the heat shock 70-kilodalton protein family, the 73-kilodalton constitutive heat shock protein. Several possible mechanisms of selective protein transport into lysosomes and the possible relevance of this proteolytic pathway to the processing of the amyloid precursor protein are discussed.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>1288371</pmid><doi>10.1111/j.1749-6632.1992.tb27477.x</doi><tpages>7</tpages></addata></record> |
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| ispartof | Annals of the New York Academy of Sciences, 1992-12, Vol.674 (1), p.58-64 |
| issn | 0077-8923 1749-6632 |
| language | eng |
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| source | MEDLINE; Access via Wiley Online Library |
| subjects | Alzheimer Disease - metabolism Amino Acid Sequence Animals Cytosol - metabolism Heat-Shock Proteins - metabolism Humans Lysosomes - metabolism Molecular Sequence Data Proteins - metabolism Ribonuclease, Pancreatic |
| title | Selective Degradation of Cytosolic Proteins by Lysosomes |
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