Locking and Unlocking of Ribosomal Motions
During the ribosomal translocation, the binding of elongation factor G (EF-G) to the pretranslocational ribosome leads to a ratchet-like rotation of the 30S subunit relative to the 50S subunit in the direction of the mRNA movement. By means of cryo-electron microscopy we observe that this rotation i...
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| Veröffentlicht in: | Cell 2003-07, Vol.114 (1), p.123-134 |
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| container_title | Cell |
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| creator | Valle, Mikel Zavialov, Andrey Sengupta, Jayati Rawat, Urmila Ehrenberg, Måns Frank, Joachim |
| description | During the ribosomal translocation, the binding of elongation factor G (EF-G) to the pretranslocational ribosome leads to a ratchet-like rotation of the 30S subunit relative to the 50S subunit in the direction of the mRNA movement. By means of cryo-electron microscopy we observe that this rotation is accompanied by a 20 Å movement of the L1 stalk of the 50S subunit, implying that this region is involved in the translocation of deacylated tRNAs from the P to the E site. These ribosomal motions can occur only when the P-site tRNA is deacylated. Prior to peptidyl-transfer to the A-site tRNA or peptide removal, the presence of the charged P-site tRNA locks the ribosome and prohibits both of these motions. |
| doi_str_mv | 10.1016/S0092-8674(03)00476-8 |
| format | Article |
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| source | MEDLINE; Cell Press Free Archives; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; ScienceDirect Journals (5 years ago - present) |
| subjects | Binding Sites - genetics Cryoelectron Microscopy Eukaryotic Cells - metabolism Eukaryotic Cells - ultrastructure Models, Molecular Molecular Conformation Peptide Elongation Factor G - genetics Peptide Elongation Factor G - metabolism Peptides - genetics Peptides - metabolism Protein Biosynthesis - genetics Protein Structure, Tertiary - genetics Protein Subunits - genetics Protein Subunits - metabolism Ribosomes - genetics Ribosomes - metabolism Ribosomes - ultrastructure RNA, Messenger - genetics RNA, Messenger - metabolism RNA, Messenger - ultrastructure RNA, Transfer - genetics RNA, Transfer - metabolism RNA, Transfer - ultrastructure Rotation |
| title | Locking and Unlocking of Ribosomal Motions |
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