Presence and release of immunoreactive atrial natriuretic peptide in granulosa cells of the pig ovarian follicle
Atrial natriuretic peptide (ANP) has been reported to be locally synthesized in the ovary although its physiological roles are still unknown. To define the origin of ovarian ANP, we demonstrated the presence and release of immunoreactive (ir) ANP in pig granulosa cells and characterized its biochemi...
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| Veröffentlicht in: | Regulatory peptides 1992-12, Vol.42 (3), p.153-162 |
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| creator | Kim, Suhn Hee Cho, Kyung Woo Lim, Seong Hee Hwang, Yun Ha Ryu, Hoon Oh, Seon Hee Seul, Kyung Hwan Jeong, Goo Bo Yoon, Sik |
| description | Atrial natriuretic peptide (ANP) has been reported to be locally synthesized in the ovary although its physiological roles are still unknown. To define the origin of ovarian ANP, we demonstrated the presence and release of immunoreactive (ir) ANP in pig granulosa cells and characterized its biochemical properties. Serial dilution curves made with the extracts of pig granulosa cells, their perfusates and follicular fluid were paralleled to the standard curve of ANP. The amount of irANP in the granulosa cell was 2 fg/cell. The total amount of irANP in granulosa cells significantly correlated with the levels of irANP in follicular fluid. Additionally, the total content of irANP in the follicle negatively correlated with the follicular size. On reverse phase HPLC, the major form of irANP in granulosa cells and follicular fluid was high molecular weight but that in perfusate was low molecular weight. In Northern blot analysis, ANP mRNA was detected in the pig granulosa cells. Immunohistochemistry showed ANP prohormone location in granulosa cells of rat ovary. These data strongly suggest that the granulosa cells synthesize and secrete ANP. |
| doi_str_mv | 10.1016/0167-0115(92)90095-C |
| format | Article |
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To define the origin of ovarian ANP, we demonstrated the presence and release of immunoreactive (ir) ANP in pig granulosa cells and characterized its biochemical properties. Serial dilution curves made with the extracts of pig granulosa cells, their perfusates and follicular fluid were paralleled to the standard curve of ANP. The amount of irANP in the granulosa cell was 2 fg/cell. The total amount of irANP in granulosa cells significantly correlated with the levels of irANP in follicular fluid. Additionally, the total content of irANP in the follicle negatively correlated with the follicular size. On reverse phase HPLC, the major form of irANP in granulosa cells and follicular fluid was high molecular weight but that in perfusate was low molecular weight. In Northern blot analysis, ANP mRNA was detected in the pig granulosa cells. Immunohistochemistry showed ANP prohormone location in granulosa cells of rat ovary. These data strongly suggest that the granulosa cells synthesize and secrete ANP.</description><identifier>ISSN: 0167-0115</identifier><identifier>EISSN: 1873-1686</identifier><identifier>DOI: 10.1016/0167-0115(92)90095-C</identifier><identifier>PMID: 1289975</identifier><identifier>CODEN: REPPDY</identifier><language>eng</language><publisher>Shannon: Elsevier B.V</publisher><subject>Animals ; Atrial Natriuretic Factor - analysis ; Atrial Natriuretic Factor - metabolism ; Atrial natriuretic peptide ; Biological and medical sciences ; Blotting, Northern ; Chromatography, High Pressure Liquid ; Estradiol - analysis ; Female ; Follicular Fluid - chemistry ; Fundamental and applied biological sciences. Psychology ; Granulosa cell ; Granulosa Cells - chemistry ; Hormone metabolism and regulation ; Immunohistochemistry ; Mammalian female genital system ; Perfusion ; RNA ; RNA, Messenger - analysis ; Swine ; Vertebrates: reproduction</subject><ispartof>Regulatory peptides, 1992-12, Vol.42 (3), p.153-162</ispartof><rights>1992</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-fe00616e350ba822436598c2fcc570c3a5d2f9d51ca3c6aa70289733724838f73</citedby><cites>FETCH-LOGICAL-c417t-fe00616e350ba822436598c2fcc570c3a5d2f9d51ca3c6aa70289733724838f73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0167-0115(92)90095-C$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4458153$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1289975$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Suhn Hee</creatorcontrib><creatorcontrib>Cho, Kyung Woo</creatorcontrib><creatorcontrib>Lim, Seong Hee</creatorcontrib><creatorcontrib>Hwang, Yun Ha</creatorcontrib><creatorcontrib>Ryu, Hoon</creatorcontrib><creatorcontrib>Oh, Seon Hee</creatorcontrib><creatorcontrib>Seul, Kyung Hwan</creatorcontrib><creatorcontrib>Jeong, Goo Bo</creatorcontrib><creatorcontrib>Yoon, Sik</creatorcontrib><title>Presence and release of immunoreactive atrial natriuretic peptide in granulosa cells of the pig ovarian follicle</title><title>Regulatory peptides</title><addtitle>Regul Pept</addtitle><description>Atrial natriuretic peptide (ANP) has been reported to be locally synthesized in the ovary although its physiological roles are still unknown. To define the origin of ovarian ANP, we demonstrated the presence and release of immunoreactive (ir) ANP in pig granulosa cells and characterized its biochemical properties. Serial dilution curves made with the extracts of pig granulosa cells, their perfusates and follicular fluid were paralleled to the standard curve of ANP. The amount of irANP in the granulosa cell was 2 fg/cell. The total amount of irANP in granulosa cells significantly correlated with the levels of irANP in follicular fluid. Additionally, the total content of irANP in the follicle negatively correlated with the follicular size. On reverse phase HPLC, the major form of irANP in granulosa cells and follicular fluid was high molecular weight but that in perfusate was low molecular weight. In Northern blot analysis, ANP mRNA was detected in the pig granulosa cells. Immunohistochemistry showed ANP prohormone location in granulosa cells of rat ovary. These data strongly suggest that the granulosa cells synthesize and secrete ANP.</description><subject>Animals</subject><subject>Atrial Natriuretic Factor - analysis</subject><subject>Atrial Natriuretic Factor - metabolism</subject><subject>Atrial natriuretic peptide</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Estradiol - analysis</subject><subject>Female</subject><subject>Follicular Fluid - chemistry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Granulosa cell</subject><subject>Granulosa Cells - chemistry</subject><subject>Hormone metabolism and regulation</subject><subject>Immunohistochemistry</subject><subject>Mammalian female genital system</subject><subject>Perfusion</subject><subject>RNA</subject><subject>RNA, Messenger - analysis</subject><subject>Swine</subject><subject>Vertebrates: reproduction</subject><issn>0167-0115</issn><issn>1873-1686</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v1DAQxS1EVZbCNyiSDwjBIWDHcRxfKqFV_0mV6KGcLXcybo2cONjJSv32ddhVucHBmsP83tP4PUJOOfvKGW-_lacqxrn8rOsvmjEtq-0rsuGdEhVvu_Y12bwgb8jbnH8xxqVS4pgc87rTWskNmW4TZhwBqR17mjCgzUijo34YljEmtDD7XdnOydtAx3UuCWcPdMJp9j1SP9KHZMclxGwpYAh51c-PSCf_QOPOFuVIXQzBQ8B35MjZkPH9YZ6Qnxfnd9ur6ubH5fX2-00FDVdz5ZCxlrcoJLu3XV03opW6g9oBSMVAWNnXTveSgxXQWqtY-ZESQtVNJzqnxAn5tPedUvy9YJ7N4PN6nR0xLtko0RRbzf8L8laWBNnq2OxBSDHnhM5MyQ82PRnOzNqIWeM2a9xG1-ZPI2ZbZB8O_sv9gP1f0b6Csv942NsMNrgSJfj8gjWN7LgUBTvbY1hC23lMJoNfi-t9QphNH_2_73gGp7WnsA</recordid><startdate>19921211</startdate><enddate>19921211</enddate><creator>Kim, Suhn Hee</creator><creator>Cho, Kyung Woo</creator><creator>Lim, Seong Hee</creator><creator>Hwang, Yun Ha</creator><creator>Ryu, Hoon</creator><creator>Oh, Seon Hee</creator><creator>Seul, Kyung Hwan</creator><creator>Jeong, Goo Bo</creator><creator>Yoon, Sik</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19921211</creationdate><title>Presence and release of immunoreactive atrial natriuretic peptide in granulosa cells of the pig ovarian follicle</title><author>Kim, Suhn Hee ; Cho, Kyung Woo ; Lim, Seong Hee ; Hwang, Yun Ha ; Ryu, Hoon ; Oh, Seon Hee ; Seul, Kyung Hwan ; Jeong, Goo Bo ; Yoon, Sik</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-fe00616e350ba822436598c2fcc570c3a5d2f9d51ca3c6aa70289733724838f73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Animals</topic><topic>Atrial Natriuretic Factor - analysis</topic><topic>Atrial Natriuretic Factor - metabolism</topic><topic>Atrial natriuretic peptide</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Estradiol - analysis</topic><topic>Female</topic><topic>Follicular Fluid - chemistry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Granulosa cell</topic><topic>Granulosa Cells - chemistry</topic><topic>Hormone metabolism and regulation</topic><topic>Immunohistochemistry</topic><topic>Mammalian female genital system</topic><topic>Perfusion</topic><topic>RNA</topic><topic>RNA, Messenger - analysis</topic><topic>Swine</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Suhn Hee</creatorcontrib><creatorcontrib>Cho, Kyung Woo</creatorcontrib><creatorcontrib>Lim, Seong Hee</creatorcontrib><creatorcontrib>Hwang, Yun Ha</creatorcontrib><creatorcontrib>Ryu, Hoon</creatorcontrib><creatorcontrib>Oh, Seon Hee</creatorcontrib><creatorcontrib>Seul, Kyung Hwan</creatorcontrib><creatorcontrib>Jeong, Goo Bo</creatorcontrib><creatorcontrib>Yoon, Sik</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Regulatory peptides</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Suhn Hee</au><au>Cho, Kyung Woo</au><au>Lim, Seong Hee</au><au>Hwang, Yun Ha</au><au>Ryu, Hoon</au><au>Oh, Seon Hee</au><au>Seul, Kyung Hwan</au><au>Jeong, Goo Bo</au><au>Yoon, Sik</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Presence and release of immunoreactive atrial natriuretic peptide in granulosa cells of the pig ovarian follicle</atitle><jtitle>Regulatory peptides</jtitle><addtitle>Regul Pept</addtitle><date>1992-12-11</date><risdate>1992</risdate><volume>42</volume><issue>3</issue><spage>153</spage><epage>162</epage><pages>153-162</pages><issn>0167-0115</issn><eissn>1873-1686</eissn><coden>REPPDY</coden><abstract>Atrial natriuretic peptide (ANP) has been reported to be locally synthesized in the ovary although its physiological roles are still unknown. To define the origin of ovarian ANP, we demonstrated the presence and release of immunoreactive (ir) ANP in pig granulosa cells and characterized its biochemical properties. Serial dilution curves made with the extracts of pig granulosa cells, their perfusates and follicular fluid were paralleled to the standard curve of ANP. The amount of irANP in the granulosa cell was 2 fg/cell. The total amount of irANP in granulosa cells significantly correlated with the levels of irANP in follicular fluid. Additionally, the total content of irANP in the follicle negatively correlated with the follicular size. On reverse phase HPLC, the major form of irANP in granulosa cells and follicular fluid was high molecular weight but that in perfusate was low molecular weight. In Northern blot analysis, ANP mRNA was detected in the pig granulosa cells. Immunohistochemistry showed ANP prohormone location in granulosa cells of rat ovary. These data strongly suggest that the granulosa cells synthesize and secrete ANP.</abstract><cop>Shannon</cop><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>1289975</pmid><doi>10.1016/0167-0115(92)90095-C</doi><tpages>10</tpages></addata></record> |
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| subjects | Animals Atrial Natriuretic Factor - analysis Atrial Natriuretic Factor - metabolism Atrial natriuretic peptide Biological and medical sciences Blotting, Northern Chromatography, High Pressure Liquid Estradiol - analysis Female Follicular Fluid - chemistry Fundamental and applied biological sciences. Psychology Granulosa cell Granulosa Cells - chemistry Hormone metabolism and regulation Immunohistochemistry Mammalian female genital system Perfusion RNA RNA, Messenger - analysis Swine Vertebrates: reproduction |
| title | Presence and release of immunoreactive atrial natriuretic peptide in granulosa cells of the pig ovarian follicle |
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