The Ret proto-oncogene in the WAG/Rij rat strain: an animal model for inherited C-cell carcinoma?
WAG/Rij rat strain has been suggested as an animal model for the study of inherited human medullary thyroid carcinoma (MTC), due to its high incidence of spontaneous C-cell thyroid tumours. Although the role of the Ret proto-oncogene mutations, as responsible for human MTC, is well established, noth...
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| Veröffentlicht in: | Laboratory animals (London) 2003-07, Vol.37 (3), p.215-221 |
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| creator | De Miguel, M Fernández-Santos, J M Trigo-Sánchez, I Matera, I Ceccherini, I Martín, I Romeo, G Galera-Davidson, H |
| description | WAG/Rij rat strain has been suggested as an animal model for the
study of inherited human medullary thyroid carcinoma (MTC), due to its high incidence
of spontaneous C-cell thyroid tumours. Although the role of the Ret
proto-oncogene mutations, as responsible for human MTC, is well established, nothing
has been published concerning this putative animal model. Based upon the previously
reported rat Ret sequence, exons 10, 11, 13, 14, 15, and 16, known
to carry activating mutations in humans, have been analysed in the
WAG/Rij rat by PCR, single strand conformational polymorphism
(SSCP) and direct sequencing. Neither the germline nor MTC samples showed any
Ret sequence difference in the exons when analysed in comparison
to a non-MTC-susceptible rat strain. Our results indicate that Ret
exons relevant in humans are not involved in WAG/Rij rat MTC, as
expected, and this questions the validity of this strain as a model for the human
disease, and suggests there must be additional mechanisms for the genesis and
progression of rat MTC. |
| doi_str_mv | 10.1258/002367703766453065 |
| format | Article |
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study of inherited human medullary thyroid carcinoma (MTC), due to its high incidence
of spontaneous C-cell thyroid tumours. Although the role of the Ret
proto-oncogene mutations, as responsible for human MTC, is well established, nothing
has been published concerning this putative animal model. Based upon the previously
reported rat Ret sequence, exons 10, 11, 13, 14, 15, and 16, known
to carry activating mutations in humans, have been analysed in the
WAG/Rij rat by PCR, single strand conformational polymorphism
(SSCP) and direct sequencing. Neither the germline nor MTC samples showed any
Ret sequence difference in the exons when analysed in comparison
to a non-MTC-susceptible rat strain. Our results indicate that Ret
exons relevant in humans are not involved in WAG/Rij rat MTC, as
expected, and this questions the validity of this strain as a model for the human
disease, and suggests there must be additional mechanisms for the genesis and
progression of rat MTC.</description><identifier>ISSN: 0023-6772</identifier><identifier>EISSN: 1758-1117</identifier><identifier>DOI: 10.1258/002367703766453065</identifier><identifier>PMID: 12869284</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Animals ; Carcinoma, Medullary - genetics ; Disease Models, Animal ; Exons ; Female ; Germ-Line Mutation ; Humans ; Male ; Mutation ; Polymerase Chain Reaction ; Polymorphism, Single-Stranded Conformational ; Proto-Oncogene Proteins - genetics ; Proto-Oncogene Proteins c-ret ; Rats ; Rats, Mutant Strains ; Rats, Sprague-Dawley ; Receptor Protein-Tyrosine Kinases - genetics ; Sequence Alignment ; Thyroid Neoplasms - genetics</subject><ispartof>Laboratory animals (London), 2003-07, Vol.37 (3), p.215-221</ispartof><rights>Laboratory Animals Ltd. 2003</rights><rights>Copyright Royal Society of Medicine Press Ltd. Jul 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c366t-dd5e4fe5fb713ca41f9d5ce4fdf3a92c558b055f2f8dd5530d50c3ae7225329f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12869284$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>De Miguel, M</creatorcontrib><creatorcontrib>Fernández-Santos, J M</creatorcontrib><creatorcontrib>Trigo-Sánchez, I</creatorcontrib><creatorcontrib>Matera, I</creatorcontrib><creatorcontrib>Ceccherini, I</creatorcontrib><creatorcontrib>Martín, I</creatorcontrib><creatorcontrib>Romeo, G</creatorcontrib><creatorcontrib>Galera-Davidson, H</creatorcontrib><title>The Ret proto-oncogene in the WAG/Rij rat strain: an animal model for inherited C-cell carcinoma?</title><title>Laboratory animals (London)</title><addtitle>Lab Anim</addtitle><description>WAG/Rij rat strain has been suggested as an animal model for the
study of inherited human medullary thyroid carcinoma (MTC), due to its high incidence
of spontaneous C-cell thyroid tumours. Although the role of the Ret
proto-oncogene mutations, as responsible for human MTC, is well established, nothing
has been published concerning this putative animal model. Based upon the previously
reported rat Ret sequence, exons 10, 11, 13, 14, 15, and 16, known
to carry activating mutations in humans, have been analysed in the
WAG/Rij rat by PCR, single strand conformational polymorphism
(SSCP) and direct sequencing. Neither the germline nor MTC samples showed any
Ret sequence difference in the exons when analysed in comparison
to a non-MTC-susceptible rat strain. Our results indicate that Ret
exons relevant in humans are not involved in WAG/Rij rat MTC, as
expected, and this questions the validity of this strain as a model for the human
disease, and suggests there must be additional mechanisms for the genesis and
progression of rat MTC.</description><subject>Animals</subject><subject>Carcinoma, Medullary - genetics</subject><subject>Disease Models, Animal</subject><subject>Exons</subject><subject>Female</subject><subject>Germ-Line Mutation</subject><subject>Humans</subject><subject>Male</subject><subject>Mutation</subject><subject>Polymerase Chain Reaction</subject><subject>Polymorphism, Single-Stranded Conformational</subject><subject>Proto-Oncogene Proteins - genetics</subject><subject>Proto-Oncogene Proteins c-ret</subject><subject>Rats</subject><subject>Rats, Mutant Strains</subject><subject>Rats, Sprague-Dawley</subject><subject>Receptor Protein-Tyrosine Kinases - genetics</subject><subject>Sequence Alignment</subject><subject>Thyroid Neoplasms - genetics</subject><issn>0023-6772</issn><issn>1758-1117</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kF1LwzAUhoMobk7_gBcSvPCuLh9Nk3ojY_gFA2FMvCxZerJ1tM1M2gv_vRkbDBSEwCE5z3lz3heha0ruKRNqTAjjmZSEyyxLBSeZOEFDKoVKKKXyFA13QBIJNkAXIWzilaaKnKMBZSrLmUqHSC_WgOfQ4a13nUtca9wKWsBVi7vY-Zy8jOfVBnvd4dB5XbUPWLfxVI2uceNKqLF1PuJr8FUHJZ4mBuoaG-1N1bpGP16iM6vrAFeHOkIfz0-L6Wsye395m05mieFZ1iVlKSC1IOxSUm50Sm1eChOfSst1zowQakmEsMyqiEa3pSCGa5CMCc5yy0fobq8bnXz1ELqiqcJuF92C60MheaooYzKCt7_Ajet9G3crGCVKZUTwCLE9ZLwLwYMttj569t8FJcUu_eJv-nHo5qDcLxsojyOHuCMw3gNBr-D47T-SPwPUi4s</recordid><startdate>20030701</startdate><enddate>20030701</enddate><creator>De Miguel, M</creator><creator>Fernández-Santos, J M</creator><creator>Trigo-Sánchez, I</creator><creator>Matera, I</creator><creator>Ceccherini, I</creator><creator>Martín, I</creator><creator>Romeo, G</creator><creator>Galera-Davidson, H</creator><general>SAGE Publications</general><general>Sage Publications Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20030701</creationdate><title>The Ret proto-oncogene in the WAG/Rij rat strain: an animal model for inherited C-cell carcinoma?</title><author>De Miguel, M ; Fernández-Santos, J M ; Trigo-Sánchez, I ; Matera, I ; Ceccherini, I ; Martín, I ; Romeo, G ; Galera-Davidson, H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c366t-dd5e4fe5fb713ca41f9d5ce4fdf3a92c558b055f2f8dd5530d50c3ae7225329f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Carcinoma, Medullary - genetics</topic><topic>Disease Models, Animal</topic><topic>Exons</topic><topic>Female</topic><topic>Germ-Line Mutation</topic><topic>Humans</topic><topic>Male</topic><topic>Mutation</topic><topic>Polymerase Chain Reaction</topic><topic>Polymorphism, Single-Stranded Conformational</topic><topic>Proto-Oncogene Proteins - genetics</topic><topic>Proto-Oncogene Proteins c-ret</topic><topic>Rats</topic><topic>Rats, Mutant Strains</topic><topic>Rats, Sprague-Dawley</topic><topic>Receptor Protein-Tyrosine Kinases - genetics</topic><topic>Sequence Alignment</topic><topic>Thyroid Neoplasms - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>De Miguel, M</creatorcontrib><creatorcontrib>Fernández-Santos, J M</creatorcontrib><creatorcontrib>Trigo-Sánchez, I</creatorcontrib><creatorcontrib>Matera, I</creatorcontrib><creatorcontrib>Ceccherini, I</creatorcontrib><creatorcontrib>Martín, I</creatorcontrib><creatorcontrib>Romeo, G</creatorcontrib><creatorcontrib>Galera-Davidson, H</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Laboratory animals (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>De Miguel, M</au><au>Fernández-Santos, J M</au><au>Trigo-Sánchez, I</au><au>Matera, I</au><au>Ceccherini, I</au><au>Martín, I</au><au>Romeo, G</au><au>Galera-Davidson, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Ret proto-oncogene in the WAG/Rij rat strain: an animal model for inherited C-cell carcinoma?</atitle><jtitle>Laboratory animals (London)</jtitle><addtitle>Lab Anim</addtitle><date>2003-07-01</date><risdate>2003</risdate><volume>37</volume><issue>3</issue><spage>215</spage><epage>221</epage><pages>215-221</pages><issn>0023-6772</issn><eissn>1758-1117</eissn><abstract>WAG/Rij rat strain has been suggested as an animal model for the
study of inherited human medullary thyroid carcinoma (MTC), due to its high incidence
of spontaneous C-cell thyroid tumours. Although the role of the Ret
proto-oncogene mutations, as responsible for human MTC, is well established, nothing
has been published concerning this putative animal model. Based upon the previously
reported rat Ret sequence, exons 10, 11, 13, 14, 15, and 16, known
to carry activating mutations in humans, have been analysed in the
WAG/Rij rat by PCR, single strand conformational polymorphism
(SSCP) and direct sequencing. Neither the germline nor MTC samples showed any
Ret sequence difference in the exons when analysed in comparison
to a non-MTC-susceptible rat strain. Our results indicate that Ret
exons relevant in humans are not involved in WAG/Rij rat MTC, as
expected, and this questions the validity of this strain as a model for the human
disease, and suggests there must be additional mechanisms for the genesis and
progression of rat MTC.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>12869284</pmid><doi>10.1258/002367703766453065</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0023-6772 |
| ispartof | Laboratory animals (London), 2003-07, Vol.37 (3), p.215-221 |
| issn | 0023-6772 1758-1117 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73481227 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Carcinoma, Medullary - genetics Disease Models, Animal Exons Female Germ-Line Mutation Humans Male Mutation Polymerase Chain Reaction Polymorphism, Single-Stranded Conformational Proto-Oncogene Proteins - genetics Proto-Oncogene Proteins c-ret Rats Rats, Mutant Strains Rats, Sprague-Dawley Receptor Protein-Tyrosine Kinases - genetics Sequence Alignment Thyroid Neoplasms - genetics |
| title | The Ret proto-oncogene in the WAG/Rij rat strain: an animal model for inherited C-cell carcinoma? |
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